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PubMed1977

[Fundamentals of anti-NADase reaction in A-streptococcus infections and its determination in microtiter test (author's transl)].

A-streptococci of known serotypes were investigated for their ability to produce NADase. For this, a microtiter-dilution technique was employed. The results were compared with those of Lazarides and Bernheimer who had determined the NADase-activity in an other way. It seems, that there is no fixed relation of NADase-production and growth curve for all of the strains. At the moment, anti-NADase can be determined only with a macrotechnique, which is, with respect to costs, time work and others, difficult to handle. A microtiter-technique is described, which is adapted to the macrotechnique. With 426 young men in the age of 18-20 years titers were found between less than 50 and 800 with a 95% level between 200 and 300. The reproducibility of the micromethod is like that of the macromethod. It is of great importance to redetermine the titer in the upper limit of normal and those which are expected to rise. The possibility to seize also infections with G- and C-streptococci is mentioned.

Adolescent↗
PubMed1978

[Group B streptococcus infections during the neonatal period (author's transl)].

Nowadays, in severe infections during the neonatal period new bacteria--group B streptococci--have to be taken into account, since in some clinics they already predominate over gramnegative rods. Septicemia and meningitis may be caused by group B streptococci. The septicemia which especially threatents prematures starts with apnoeic spells in the very first hours after birth and may be easily misdiagnosed as an idiopathic respiratory distress syndrome. The mortality is very high (about 60%). Meningitis starts later, normally during the 3rd to 4th week. Seizures are typical at the onset. Group B streptococci may be identified in the CSF by counterimmunoelectrophoresis within one hour. The prognosis is more favourable in meningitis than in septicemia (mortality about 20%). Survivors have little neurological sequelae. Penicillin G or ampicillin combination with an aminoglycoside is recommended as chemotherapy. Exchange transfusion should be considered early. Group B streptococci causing the septic form may be transfered during labour since up to 25% of pregnant women are colonized. Nosocomial transmission of group B streptococci may be the reason for meningitis. Prophylactic penicillin does not seem to help in preventing the disease, but it is possible, that meningitis of the newborn may be prevented by immunizing the mother during pregnancy.

Age Factors↗