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At least 55 records · Page 3Linked to original sources
PubMed1978

[Restriction of washing and its effect to the normal human skin flora. Quantitative and qualitative investigations of the aerobic skin flora (author's transl)].

The behaviour of the aerobic skin flora of the flexor sides of the forearms, under a three-week restriction of washing, was investigated in twenty-four patients for its quantitative and qualitative aspects. The combined scrabbing-washing method was used as described by Burtenshaw with Ringer's solution. After a three-week restriction of washing, using the Wilcoxon Test, statistically no significant changes in the total number of microorganisms were found in areas investigated in the intermittent sampling of the skin flora. A three-week restriction of washing in the region of the flexor sides of the forearms does not induce significant changes in the composition of the normal aerobic skin flora. The percentage of differences in the total number of bacteria fluctuates up to the mark in wide limits; statements about standard deviations are not permitted because of the technical and experimental conditions. There was no shifting of the Resident flora to other groups of bacteria in the patients under study. Pathogenic microorganisms could not be found in higher numbers after the second sampling.

Actinomycetales↗
PubMed1975

[Skin transplantation in the study of chemical carcinogenesis. III. Reduced papilloma-formation after initiation during epidermal hyperplasia induced by skin grafting or by a single application of the cocarcinogen TPA].

Skin autografting or a single painting with the cocarcinogen TPA was used to induce epidermal hyperplasia in the back skin of C3H mice. Initiation by intragastric application of the carcinogen DMBA during this state of hyperplasia and subsequent promotion by repeated application of the cocarcinogen TPA led to decreased papilloma-formation, as compared to mice of a control group which had not been pretreated before initiation. Reports by others referring to increased susceptibility of replicating epidermal cells to the effect of initiation thus cannot be confirmed. The reduction of papilloma-formation can most probably be ascribed to effects of local inflammation, either preferentially but unspecifically damaging initiated cells, or facilitating a specific immune response against tumor-associated transplantation antigens of prospective tumor cells.

9,10-Dimethyl-1,2-benzanthracene↗
PubMed1979

Reflex control of skin blood flow by skin temperature: role of core temperature.

Two protocols were used to discover whether the reflex response in skin blood flow (SkBF) to rising skin temperature (Tsk) was dependent on the level of internal temperature. Part I. In five subjects, Tsk (controlled with water-perfused suits) was raised to 37 degrees C prior to, between 2 and 5 min, or between 10 and 17 min of exercise. The associated SkBF elevation per degree rise in Tsk averaged 0.20, 1.28, and 1.75 ml/100 ml . min, respectively. When Tsk was raised during the first 5 min of exercise, esophageal temperature (Tes) rose markedly (0.39 degrees C), but transiently fell if Tsk was raised after 10 min of exercise. Part II. In six subjects, different work loads were used to develop different levels of internal temperature. Tsk was elevated to 37 degrees C after 10--15 min at light (50--75 W) or moderate (100--150 W) work loads. At the heavier work load (and higher Tes), the rise in forearm SkBF per degree rise in Tsk averaged 2.33 +/- 0.38 (SE) times that observed at the light work load. These data strongly suggest that the reflex response of SkBF to rising Tsk is dependent on the level of internal temperature.

Adult↗
PubMed1977

Immunofluorescence skin test for lupus erythematosus. A correlative study between frequency and patterns of skin test results, glomerular pathologic abnormalities, and immunologic findings.

We undertook a correlative study between the frequency and patterns of immunoglobulin deposits at the dermoepidermal junction of clinically normal skin and the morphologic features (histopathologic and immunopathologic) of the glomerular lesions and various immunologic measurements in 38 patients with systemic lupus erythematosus. Thirty-five (92.1%) showed a positive immunoglobulin band at the skin. A statistically significant (P less than .001) correlation was observed between the pattern of deposit and the duration of disease. The stippled pattern was found to be more common in those cases of less than one year's duration, while the thready pattern was more common in those cases lasting longer than one year. There seemed to be no significant relationship between either the frequency (P greater than .100) and pattern (P greater than 0.25) of the immunoglobulin band and the glomerular pathologic or other immunologic measurements.

Complement C3↗
PubMed1977

Pemphigus antibody action on skin explants: kinetics of acantholytic changes and stability of antigens in tissue cultures of normal monkey skin explants.

The pathogenic effect of the intercellular antibodies of pemphigus was studied by using organ culture of monkey skin. Skin explants that were grown on sera with intercellular antibody titers of 320 or greater fixed these antibodies within one day as demonstrated by direct immunofluorescence for IgG. None of the control sera gave such staining patterns. Following the binding of intercellular antibodies, characteristic histologic changes appeared, notably separation of individual epidermal cells and acantholysis. These histologic changes became more marked in two to five days. During this period, the bound antibodies and the intercellular antigens decreased and disappeared. These temporal relationships of immunofluorescence and histologic findings suggest that pemphigus antibodies play a role in the induction of acantholysis.

Acantholysis↗
PubMed1979

Nitrosomethylurea (MNU): a powerful carcinogen for mouse skin. A study utilising the tetrazolium test (TZT) and topical skin applications.

Nitrosomethylurea (MNU) was tested with the tetrazolium test for skin carcinogens with positive results. 39 hairless mice were painted once a week with a 1% solution of MNU in acetone. After 18 weeks all the animals bore tumours and 90% had skin carcinomas. Hence, the strong carcinogenic potency of MNU was confirmed, and the validity of the tetrazolium test was given further support.

Animals↗
PubMed1979

Uptake of 3H-testosterone in the skin of healthy women and in the skin of patients with acne vulgaris.

Skin samples of interscapular region were taken from healthy women and patients with Acne vulgaris. The material was incubated with 3H-testosterone. The incubation of a part of the material was preceeded by an incubation with the unlabelled testosterone. An autoradiography was employed to detect and localize an uptake of 3H-testosterone in the skin. A significantly greater 3H-testosterone uptake was noted in the stratum basale and stratum spinosum of epidermis, sebaceous glands and hair sheaths and, in patients with Acne vulgaris, also within hair sacs and comedons.

Acne Vulgaris↗
PubMed1978

Comparison between the early use of skin flap and skin graft for the correction of large tissue loss at the elbow.

Six patients with soft and hard tissue injuries of the elbow region during the 1973 Yom-Kippur war are discussed. These six patients were treated with two types of procedures. One group was initially treated with application of a split-thickness skin graft followed by a thoracoabdominal, abdominal, or chest skin flap. The second group was initially treated with the application of a thoracoabdominal flap. There are few descriptions in the literature or in textbooks of the plan design method, nor are there comparisons of two types of procedures. In both groups the end results were the same. The differences were the time of hospitalization and the cooperation of the patients during treatment. In both groups the flap was transferred before further surgical reconstruction of bones and joints.

Adult↗
PubMed1975

alpha-N-benzoylarginine-2-naphthylamide hydrolase (cathepsin B1 ?) from rat skin. II. Purification of the enzyme and demonstration of two inhibitors in the skin.

A thiol-activated, alpha-N-benzoylarginine-2-naph-thylamide (BANA) hydrolyzing enzyme was purified from rat skin by ammonium sulfate precipitation, gel filtration, and DEAE cellulose chromatography. In DEAE cellulose chromatography the enzyme was fractionated into two multiple forms (preparations I and II). The activity of undiluted preparation II, but not that of preparation I, was increased, when the enzyme was preincubated at pH 4, at 55 degrees C. Simultaneously, the isoelectric point of preparation II was shifted to that of preparation I, i.e., from 6.2 to 7.5. Activated preparation II behaved in DEAE cellulose chromatography as preparation I. Molecular weights of the enzymes of both preparations were 27 000, and pH optima were at pH 5.8 and 7.0, for BANA and leucine-2-napthylamide (Leu-NA), respectively. The BANA and Leu-NA hydrolyzing enzymes could not be separated by gel filtration, DEAE, CM, or Amberlite IRC-50 chromatography, isoelectric focusing, or analytical polyacrylamide gel electrophoresis. Two inhibitors of BANA hydrolase were demonstrated by gel filtration in the salt precipitated skin extract. The activities of the BANA hydrolase preparations did not increase linearly with increasing enzyme concentration, with the exceptions of activities of preparation I and acid-activated preparation II. The role of the inhibitors in the nonlinearity of the activity/enzyme concentration curves is discussed.

Aminopeptidases↗
PubMedDate not supplied

[Interactions of Shope papilloma virus with SV40 in adult domestic rabbit cell lines derived from normal skin or from Shope papilloma virus infected skin (author's transl)].

Three cell lines from adult domestic rabbit are infected with SV40: LP 17 and LP 45 derived from normal skin and LP 42 PS derived from skin infected for 24 h with Shope papilloma virus. The fibroblastic morphology of the cultures is not changed. SV40 is not recovered and T antigen is only detected in LP 42 PS cell line after 29 passages. To know if Shope papilloma virus facilitates penetration of SV40, cultures of LP 45 are first infected with Shope papilloma virus for 2 h and for 24 h, then superinfected with SV40. There is no cellular alteration, and T antigen induced by SV40 is only detected in cells pretreated for 24 h with Shope papilloma virus, after 20 passages. When cultures LP 45 are infected with Shope papilloma virus neutralized with a high titer antiserum and superinfected with SV40, T antigen is not detected. Superinfected cells containing specific SV40 T antigen do not induce tumors either in new born hamsters or in rabbits but they are able to grow in colonies in soft agar. LP 42 PS cell line and LP 45 cells infected with Shope papilloma virus for 24 h containe Shope papilloma virus genome which is able to modify the permissivity of rabbit cells to SV40.

Animals↗
PubMed1979

[Utilization of a synthetic skin substitute instead of split skin in cross-leg-flap and other pedicle flap grafting].

The method using synthetic skin substitute Epigard as a long-term coverage for donor sites in cross-leg-flap- and other plastic surgery procedures is described. The double-layered polyurethane/Teflon-material can be left on the wound for several weeks to avoid bacterial contamination during holding periods and to prepare regrafting of skin flaps. Experiences based on 32 cases are described.

Bandages↗
PubMed1978

The chemical mediation of delayed hypersensitivity skin reaction. II. Characterization of a macrophage-chemotactic factor from bovine gamma-globulin-induced skin reaction in guinea pigs.

Macrophage-chemotactic factors were extracted from delayed hypersensitivity skin lesions induced by bovine gamma-globulin in guinea pigs. The most active factor, MCFS--1, was highly purified and found to be a heat-labile protein with a molecular weight of 150,000 and to possess in vivo as well as in vitro activity. This factor was homogeneous during polyacrylamide gel electrophoresis, and the chemotactic activity was associated exclusively with this band. Further characterization revealed that its isoelectric point was 6.7 to 6.9 and made a single arc in the beta-globulin region with rabbit antiserums against guinea pig serum on immunoelectrophoresis. This factor seemed to be antigenically different from immunoglobulin G (IgG) by immunodiffusion and immunoadsorption. On the other hand, the chemotactic activity of MCFS-2 was adsorbed by neither anti-IgG nor anti MCFS-1 and that of euglobulin fraction was partially adsorbed by anti-IgG. These indicate the presence of at least three types of antigenically different chemotactic factors for macrophages in the extracts of delayed hypersensitivity skin lesions.

Animals↗
PubMed1978

[Improvement of healing of pedicled skin flaps and free skin grafts (animal experiments)].

A combined therapy in order to improve the healing of dermatoplasties is presented. Epicutaneous application of oxygen is combined with injection of a vasodilating drug. The results from experiments on 25 rabbits showed accelerated healing, smaller necroting areas, and improved circulation in pedicled skin flaps of well as in free cutaneous grafts. All results are statistically secured and are confirmed by histologic examinations. The method is uncomplicated and very effective.

Animals↗
PubMed1979

The main neutral protease of rat skin is a mast cell enzyme. Immunohistochemical localization of the enzyme in rat skin with the peroxidase-antiperoxidase (PAP) complex method.

The highly sensitive PAP immunoperoxidase method was used to localize the main neutral protease of rat skin. The use of the neutral detergent, Triton X-100, in the reagent and washing solutions was observed to effectively decrease the nonspecific staining. The specific staining was localized to the mast cell granules.

Animals↗