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PubMed1998

A scanning electron microscopic study of tooth surface changes induced by tannic acid.

STATEMENT OF PROBLEM: Exposing the tooth structure to chemicals used in displacing the gingival tissue is inevitable. PURPOSE: This study was undertaken to microscopically examine the effect of tannic acid on the prepared dentin surface. MATERIAL AND METHODS: Forty recently extracted intact human teeth were mounted for buccal surface preparation. Teeth were equally divided into 10 groups. One group was kept as a control and the other teeth received a topical application of 15%, 20%, and 25% aqueous tannic acid solutions each for 5, 10, and 15 minutes at room temperature. All samples were washed, air-dried, then prepared for scanning electron microscopic examination. Two different areas in the cervical region were randomly selected and examined under magnifications of x1000 and x2000, then photographed. The assessment of the changes was performed qualitatively. RESULTS: The results showed an incomplete removal of the smear layer in all experimental samples. The astringent action of the tannic acid solution on both the smear layer and the surface peritubular dentin around the orifices of dentinal tubules had contributed to their constriction. The degree of constriction of the orifices of dentinal tubules increased as higher concentrations of tannic acid solutions were used and as the application time was further increased at each concentration. CONCLUSION: The tannic acid had an incomplete action on the removal of the smear layer. It also seemed to have an astringent action on both the smear layer and the peritubular dentin.

Astringents↗

PubMed1998

A study of the classification and treatment of noncarious cervical lesions by general practitioners.

PURPOSE: This study presented a survey to determine what general practitioners in Nova Scotia perceived to be the cause of noncarious cervical lesions, and to ascertain their most frequent method of treatment. METHODS: The survey was completed by 63% of the 343 dentists polled. RESULTS: Ninety-four percent classified the lesion as abrasion, and 66% rated toothbrushing as the most likely cause. Treatment methods were varied, with no clear preference. CONCLUSION: The most frequently used restorative materials were GI/composites (29%) and composites (27%). The results of the survey suggest that treatment provided for noncarious cervical lesions may not be based on the correct diagnosis.

Clinical Competence↗

PubMed1996

pH changes at the surface of root dentin when using root canal sealers containing calcium hydroxide.

The purpose of this study was to investigate long-term pH changes in cavities prepared in root surface dentin of extracted teeth after obturation of the root canal with gutta-percha and a variety of sealers containing calcium hydroxide. After cleaning and shaping, root canals in 50 recently extracted, human single-rooted teeth were divided into five groups. Each of four groups was obturated with gutta-percha and either Sealapex, Sealer 26, Apexit, or CRCS, all of which contain calcium hydroxide. The remaining group served as the control and was not obturated with gutta-percha or sealer. Cavities were prepared in the facial surface of the roots in the cervical and middle regions. The pH was measured in these dentinal cavities at the initiation of the experiment, and 3, 7, 14, 21, 28, 45, 60, 90, and 120 days after obturation. Results indicate that the pH at the surface of the root does not become alkaline when calcium hydroxide cements are used as root canal sealers. Regardless of the sealer used, the observed pattern of pH change was not different from that seen in the control group of roots that were not treated with sealer. It is concluded that calcium hydroxide-containing cements, although suitable for use as root canal sealants, do not produce an alkaline pH at the root surface. If such a pH change is related to treatment of root resorption, these sealants do not contribute to this treatment.

Bismuth↗

PubMed1996

Changes in pH at the dentin surface in roots obturated with calcium hydroxide pastes.

The purpose of this study was to determine the pH, after defined periods of time, in cavities prepared in the facial surface of the cervical, middle, and apical regions of roots obturated with calcium hydroxide pastes. Root canal instrumentation was performed on 40 recently extracted, single-rooted human teeth. Cavities 1.5 mm in diameter and 0.75 mm in depth were prepared in the cervical, middle, and apical regions of the facial surface of each root. Teeth were randomly divided into four groups. One group was left unobturated and served as a control. The three remaining groups were obturated with either aqueous calcium hydroxide, calcium hydroxide mixed with camphorated monochlorophenol. or Pulpdent pastes. Access cavities and apical foramina were closed with Cavit. Each tooth was stored individually in a vial containing unbuffered isotonic saline. pH at the surface was measured in the cervical, middle, and apical cavities at 0 and 3, 7, 14, 21, 28, 45, 60, 90, and 120 days. Results indicate that hydroxyl ions derived from calcium hydroxide pastes diffused through root dentin at all regions over the experimental period of 120 days. The pattern of pH change at the tooth surface was similar in all regions of the root, regardless of the type of calcium hydroxide paste used. This was a rapid rise in pH from a control value of pH 7.6, to greater than pH 9.5 by 3 days, followed by a small decline to pH 9.0 over the next 18 days, before finally rising and remaining at, or above pH 10.0 for the remainder of the experimental period. Pulpdent paste in the apical region was the only exception in this pattern, producing a pH rise nearly one full unit below the other pastes, pH 9.3. These results indicate that, for all pastes tested, a high pH is maintained at the root surface for at least 120 days.

Analysis of Variance↗

PubMed1998

Strengthening immature teeth during and after apexification.

The purpose of this study was to evaluate the strengthening effect of an internal resin bonding technique. One hundred central incisors were endodontically treated and divided equally into five groups. Group 1, without any cervical preparation and with access repaired with bonded resin, served as the positive control. Teeth in groups 2 to 5 were cervically prepared to simulate the thin dentinal wall of immature teeth, and obturation material was removed to 3 mm below the cementoenamel junction (CEJ). In group 2, the negative control, the access was restored with bonded composite to the level of the CEJ. In group 3, access was restored with composite resin 3 mm apical to the CEJ using a clear post system. Group 4 was treated similarly to group 3, but an opaque post replaced the clear post. Group 5 was treated like group 3, after which a metal post was cemented into the channel left after removal of the clear post. All teeth were subjected to compression testing and the force required to fracture the teeth cervically was recorded for each group and the Kruskal-Wallis analysis performed to detect intergroup differences. All of the bonded resin techniques significantly strengthened the teeth against fracture, compared with the negative control group (p < 0.05). None of the experimental groups (3-5) were significantly different from the positive control group.

Composite Resins↗

PubMed1999

Surgical extrusion of a cervically root-fractured tooth after apexification treatment.

A case is reported in which an incisor fractured below the alveolar crest 6 months after completion of apexification treatment was surgically extruded for prosthetic coronal restoration. After the surgical procedure, a dowel post was placed in the root canal, a core was built using glass-ionomer cement, and a porcelain veneer crown restoration was completed. The 24-month follow-up examination after surgical, endodontic, and prosthetic treatments showed that the tooth was clinically and radiographically healthy and functioned well.

Child↗

PubMed2000

Clinical evaluation of three adhesive systems in class V non-carious lesions.

OBJECTIVES: The purpose of this study was to evaluate the clinical retention of three new adhesive systems in non-carious cervical lesions during a 3-year period. METHODS: The adhesive systems, a three-step (EBS/Pertac Hybrid), a one-bottle resin bonding agent (One-Step/Pertac Hybrid) and a resin-modified glass ionomer cement (Fuji II LC) were placed in 148 non-carious cervical lesions, 87 with sclerotic dentin and 61 non-sclerotic. Of the sclerotic lesions treated with the two resin bonding systems, 37 were slightly roughened with a diamond bur before conditioning. The restorations were evaluated every 6 months during a 3-year period. RESULTS: All except six restorations were evaluated during the 3 years. The cumulative loss rates for EBS, One-Step and Fuji II LC were, at 1 year: 2, 24 and 2% and at 3 years: 10, 49, 7%, respectively. The one-bottle adhesive showed significantly more failures. The five lost EBS restorations were found in non-sclerotic lesions, while the three lost Fuji II LC restorations had been placed in sclerotic lesions. For the One-Step material the loss frequency for non-sclerotic versus sclerotic lesions was 31.8 and 65.2%, respectively. Slight roughening of the sclerotic dentin surfaces with a diamond bur did not increase retention of the restorations. SIGNIFICANCE: The three-step resin adhesive and the RMGIC showed clinically acceptable retention rates, while a high failure rate was registered for the one-bottle adhesive.

Adult↗

PubMed2001

Bonding of all-porcelain crowns: structural characteristics of the substrate.

OBJECTIVES: The aim of this in vitro study was to evaluate the morphology of dentin in teeth prepared for single-unit all-porcelain crowns (SUAPC) in terms of tubule orientation, density and increase in surface area after etching. METHODS: Twenty anterior and 20 posterior teeth from adults were prepared 1mm below the cemento-enamel junction (CEJ) for SUAPC. The samples were divided into groups based on type of tooth (anterior or posterior) and bonding system employed. The teeth were processed for evaluation of morphology of the substrate, hybrid layer thickness and resin tag formation. The observations were subdivided according to location in the preparation and tubule density was calculated. RESULTS: The observations revealed the variability in tubule density and orientation in different areas within any one preparation. The morphology of the cervical margin was less predictable with the presence of cementum and an ill-defined cemento-dentinal junction. Statistically significant differences in the density of tubules were found depending on location. Groups 2, 3, 5 and 6 samples showed that the hybrid layer thickness and resin tag morphology depended on the density and direction of tubules. In those sectors with parallel and oblique tubule orientation and with a low density of tubules, the hybrid layer was significantly thinner than in areas with cross-sectioned tubules. SIGNIFICANCE: The cementum and the peripheral intertubular dentin surface area are likely to be responsible for the bond strength after acid etching of crown preparations, but not all areas exhibited equal responses to etching. In particular, the bonding substrate at the gingival margins may contribute little in terms of micromechanical retention.

Acid Etching, Dental↗

PubMed1996

Bioengineering seeds of contemplation: a private practitioner's perspective.

In a precise semantic sense, the most significant biomaterial in dentistry is the human tooth. How it reacts, changes and is affected during the dynamics of occlusal loading should be reexamined in light of modern technology. Much is known about the embryology, histology, biochemistry, anatomy, and the microbiology affecting teeth; however, there are areas of biomechanics, biochemistry, and bioelectricity which also affect dental hard tissues that remain largely unexplored. Technological advances enable us to use more sophisticated instrumentation to measure and quantify changes that occur during the dynamics of occlusal activity. Armed with this information, dentistry can better understand how teeth interact with restorative materials in order to increase the longevity of both.

Biomechanical Phenomena↗

PubMed1998

Marginal adaptation and retention of a glass-ionomer, resin-modified glass-ionomers and a polyacid-modified resin composite in cervical Class-V lesions.

OBJECTIVES: An 18-month follow-up clinical trial of one conventional glass-ionomer (HIFI Master Palette), three resin-modified glass-ionomers (Fuji II LC, Vitremer, 3M Exp. 155) and one polyacid-modified resin composite (Dyract) was conducted to evaluate their clinical effectiveness in Class-V cervical lesions. In addition, the interface between dentin and two resin-modified glass-ionomers and one polyacid-modified resin composite was examined by scanning electron microscopy (SEM). METHODS: After evaluation of the restorations immediately following placement (baseline), all patients were subjected to a strict recall schedule with controls at 6, 12 and 18 months. The clinical effectiveness was recorded in terms of retention and marginal integrity, clinical microleakage, caries recurrence, and tooth vitality. A chi 2-test (p < 0.05) was used to test for significant differences between materials. In case of restoration loss or special defects, a replica was made to examine the surface texture and restoration margins by SEM. In vitro, the interface was examined by SEM after an argon-ion-beam etching technique was used to enhance surface relief and disclose interfacial substructures. RESULTS: Retention appeared to be good for all the materials tested. Marginal discrepancies were localized at the incisal enamel and/or the cervical dentin margin, except for the polyacid-modified resin composite that showed most of the defects at the incisal enamel margin. None of the systems could guarantee margins free of microleakage for a long time. In vitro, the type of dentin pre-treatment defines to a great extent the morphology of the resultant interface between dentin and the restorative material tested. SIGNIFICANCE: In this clinical study, the retention rate of the tested materials was good and even excellent for some products. Perfect marginal adaptation deteriorated too fast. The marginal adaptation of the polyacid-modified resin composite at the enamel site would probably have been better by the use of selective enamel or total acid etching. Marginal sealing remains a problem. Future research should concentrate on improving the marginal adaptation and sealing capacities before a broader clinical use can be advocated.

Acrylic Resins↗

PubMed2000

Enamel demineralisation assessed by computerised image analysis of clinical photographs.

OBJECTIVES: To investigate the use of computerised image analysis to measure the area of demineralisation on the buccal surface of a tooth and to analyse the effect on that measurement of varying the angle at which a photographic image of the buccal surface of a tooth is taken. METHODS: Twenty-two teeth with areas of incremental demineralisation on the buccal surface were photographed perpendicular to the buccal surface and at angles of 20 and 40 degrees, above (Cuspal) and below (Gingival) the perpendicular. The images were captured and analysed using computerised image analysis. RESULTS: The Gingival views showed lower limits of agreement than the Cuspal views, probably due to the positioning of the masking on the lower part of the flash, to reduce reflection. There was a significant difference between the areas of demineralisation measured for the three angles from the Gingival views (p<0.001). The mean differences in areas measured were not linear, but there was a significantly greater reduction in area measured for the 40 degrees views. CONCLUSIONS: Computerised image analysis to measure areas of demineralisation from a captured 35mm slide is reproducible. The position of the masking on the camera flash and the angle of the camera makes a difference to the area measured and the grey scales recorded. Images taken at a larger angle than 20 degrees to the perpendicular will record a significantly reduced area of demineralisation. Standardisation of photographs with the use of calibration markers and manipulation of images is discussed.

Analysis of Variance↗

PubMed2000

Penetration of radiocalcium at the margins of resin and glass ionomer dentine bonding agents in primary and permanent teeth.

OBJECTIVES: The purpose of this study was to compare the leakage of three resin dentine bonding agents (Prime and Bond, Scotchbond Multi-Purpose, Probond) and a glass ionomer dentine bonding agent (GC Fujibond LC), in cervical cavities prepared in primary and permanent molar teeth restored with a hybrid composite resin (Tetric). METHODS: Cervical cavities without a bevel at the cavo-surface margins were prepared in the buccal and lingual surfaces of extracted primary and permanent molar teeth. After being restored, the teeth were stored for 1 week in a saline solution at 37 degrees C and then thermally cycled between 5 and 55 degrees C. Marginal leakage was determined subsequently using a radioactive isotope containing 45Ca and an autoradiographic technique. RESULTS: The results revealed that there were no statistically significant differences in microleakage of the bond between permanent and primary teeth dentine and Fuji Bond LC and Probond dentine bonding agents. The difference between permanent and primary teeth groups for gingival values of the Prime and Bond 2.1 group U=22.5, p=0.0355 and the Scotchbond Multipurpose group U=24.0, p=0.0406 were statistically significant. There were no significant differences between the occlusal and gingival microleakage values in either primary or permanent teeth with Prime and Bond 2.1, Fuji Bond LC and Probond except the difference at Scotchbond Multipurpose in primary teeth. For primary teeth gingival margins, none of the bonding systems were significantly different from the control group. CONCLUSIONS: These results indicate that although no statistically significant differences were found between test and control group values, the use of Fuji II LC in cervical cavities with cementum margins in primary teeth would provide the best resistance to microleakage among the test materials while the use of Scotchbond Multi-Purpose would provide the best resistance to microleakage in cervical cavities with cementum margins in permanent teeth.

Analysis of Variance↗

PubMed2001

Eighteen-month clinical evaluation of a filled and unfilled dentin adhesive.

OBJECTIVE: The purpose of this study was to evaluate the performance of a filled (OptiBond Solo) and an unfilled (Prime & Bond 2.1) "one-bottle" adhesive in Class V restorations after 18 months of clinical service. METHODS: Thirty-three patients with non-carious cervical lesions were enrolled in the study. A total of 101 lesions were restored using one of the adhesives and a hybrid composite resin. Enamel was not beveled, nor was any mechanical retention placed. The restorations were evaluated at baseline, and at 6 and 18 months after placement using modified USPHS criteria. RESULTS: Cumulative 18-month retention rates were 93.6% for OptiBond Solo and 98.0% for Prime & Bond 2.1. The difference in retention rates was not statistically significant. For OptiBond Solo, the only notable problems were interfacial staining and marginal adaptation, both of which were less than ideal in 9% of restorations. Marginal problems were slightly less frequent for Prime & Bond 2.1 restorations, but the difference was not significant. CONCLUSIONS: Both adhesives provided Class V retention rates exceeding the 18-month, full acceptance guidelines set by the American Dental Association. Any additional benefit provided by the use of a filled adhesive was not detected in this 18-month clinical trial.

Adhesives↗

PubMedDate not supplied

Comparison of the in vitro permeability of human dentine according to the dentinal region and the composition of the simulated dentinal fluid.

OBJECTIVE: To compare the permeability of the occlusal and the cervical dentinal regions within the same tooth, which represent the bottom of Class I and V cavities, respectively, with two different compositions of perfusion fluid, in vitro. METHODS: An occlusal and a buccal disc were cut from each extracted third molar at a level close to the pulp chamber. The convective permeability of the discs was measured in a fluid transport model using de-ionised water or 1:3 diluted bovine serum and the hydraulic conductance was determined. RESULTS: The mean hydraulic conductance values of the occlusal and the buccal cervical human dentine to water were 0.069 and 0.047 microl min(-1) cm(-2) cm H(2)O(-1), respectively. When diluted bovine serum was used, the corresponding values were 0.036 and 0.012 microl min(-1) cm(-2) cm H(2)O(-1), respectively. Statistical analysis revealed no significant difference between the permeability of these two regions. The composition of the perfusion fluid significantly influenced the permeability of dentine. CONCLUSIONS: The occlusal and the buccal cervical regions of human dentine have similar permeability characteristics in vitro. There is a strong reverse correlation between the viscosity of the perfusion fluid and the perfusion through the dentine.

Animals↗

PubMedDate not supplied

Stresses at the cervical lesion of maxillary premolar--a finite element investigation.

OBJECTIVES: The objective of this study was to use a three-dimensional (3-D) finite element model to investigate normal stress distribution to substantiate the tooth flexure mechanism. The study also compared the changes in the stresses by different occlusal loading sites and directions. METHODS: The 3-D finite element analysis was used. A maxillary premolar was selected to construct the simulation model. The model was constructed step-by-step for convergence and validity. Seven load conditions for various load sites and different directions were simulated to the model. RESULTS: The maximal principal stress and minimal principal stress distributions developed within the structures of seven load conditions were output and their stress distributions on z-plane at the vertical midline were shown. The peak tensile stress of the cervical area for various load conditions were compared and listed. CONCLUSIONS: This study has shown that the presence of tensile stresses in the cervical region of a maxillary premolar by various loading sites and different directions. The results coincided with the stress-induced theory, hence sustaining it. The relationship of the affected factors of leverage to the development of cervical abfraction lesions, was explored.

Bicuspid↗

PubMedDate not supplied

Pulpal space pressure and temperature changes from Nd:YAG laser irradiation of dentin.

OBJECTIVES: To investigate pulpal space pressure and temperature after application of Nd:YAG laser, and high-speed diamond bur on dentin surface. METHODS: One and 3 W Nd:YAG laser and high-speed diamond bur were used to remove dentine from twenty extracted premolars. The pulp chambers were monitored for pressure and temperature changes with a pressure transducer and thermocouple, respectively. RESULTS: Regardless of the remaining dentin thickness (RDT), laser irradiation and high-speed diamond bur use generated an increase in pulpal space pressure and temperature (ANOVA and Fisher's LSD tests, P < 0.001). Pressure and temperature increased with an increase in laser power. Three-Watt laser irradiation caused greater changes than 1 W (1.75 kPa and 1.31 degrees C, 0.53 kPa and 0.34 degrees C, respectively). Both pulpal space pressure (P < 0.001) and temperature (P < 0.005) increased as the RDT decreased. CONCLUSIONS: Laser irradiation and the use of a high-speed diamond bur generated an increase in pulpal space pressure and temperature. Pulpal space pressure and temperature increased with an increase in energy density of laser and a decrease in RDT.

Adolescent↗

PubMed2003

The effect of magnification on the iatrogenic damage to adjacent tooth surfaces during class II preparation.

OBJECTIVES: To determine the amount of damage to adjacent surfaces during cavity preparation of approximal box-cavities by using either no magnification or an individually adapted surgical telescope. MATERIALS AND METHODS: Nine dentists prepared in a mannequin head 4 approximal (class II) box-cavities without magnification using a high speed handpiece with lighting from a dental unit. At least 2 months later the test was repeated with a surgical telescope system with integrated light. The degree of damage the 72 adjacent surfaces suffered was determined. RESULTS: The average time needed to prepare one cavity was 14.9min without and 18.3min with magnification (p=0.01). Altogether, 29.7% of adjacent area were damaged when no surgical telescopes were used and 34.5% when surgical telescopes were applied (p>0.05). When the mesial-facing and distal-facing surfaces were analysed independently a statistically significant increase of damage was found on distal surfaces (p=0.03) when using surgical telescopes. Seventy surfaces (=97%) had a preparation trauma. CONCLUSIONS: Surgical telescopes do not decrease damage of adjacent tooth surfaces.

Adult↗

PubMedDate not supplied

Effect of restored and unrestored non-carious cervical lesions on the fracture resistance of previously restored maxillary premolar teeth.

OBJECTIVES: The effect of non-carious cervical lesions (NCCL) on tooth fracture resistance has not previously been investigated. The aims of this in vitro study were to examine the fracture resistance of a group of extracted maxillary premolar teeth with mesio-occlusal-distal (MOD) restorations of amalgam, and restored or unrestored simulated NCCL. METHOD: Forty sound maxillary, premolar teeth were divided at random into four groups, each of 10 teeth, which were fixed crown uppermost and long axis vertical in stainless steel moulds. Groups 1,2,3 and 4 were prepared with standardized parallel-sided MOD cavities, then restored with amalgam. Groups 1, 2 and 3 were further prepared with standardized NCCL. The NCCL in Group 1 were restored using a resin-modified polyalkenoate (glass-ionomer) cement, and the NCCL in Group 2 were restored with an adhesive composite resin system. The NCCL in Group 3 were left unrestored. The specimens were loaded compressively at 1 mm min-1 using a universal testing machine. RESULTS: Mean fracture loads (KN) of 1.08, 1.03, 0.98 and 1.14, respectively, were recorded for Groups 1, 2, 3 and 4. Two-way ANOVA and Scheffe's Multiple Range Test showed no statistically significant difference between the groups. CONCLUSIONS: It is concluded that the presence of a standardized NCCL in an extracted maxillary premolar tooth does not reduce the fracture resistance of the tooth when loaded compressively at 1 mm min-1. The restoration of NCCL with the materials tested did not result in an increase in the fracture resistance of the previously restored premolar teeth, when loaded compressively at 1 mm min-1.

Analysis of Variance↗