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Analysing parent-child interactions in clinical practice: the development of a procedure and method of reporting.

Research in child developmental psychology is concerning itself increasingly with the fine-grain study of infant-caregiver interactions and as a result awareness of the importance of early interaction patterns in the development of children has grown very rapidly. The research has been based on an observational approach made possible by the use of video-recording and the development of sophisticated methods of interaction analysis. The methods employed and the research findings have important implications for those working clinically with young handicapped children and their parents, but as yet the research methods have been too complex and time-consuming to be used directly in practice. This paper describes the development of a procedure which can be used to observe and analyse the interactions of pre-school handicapped children with their caregivers in a clinical setting, using modifications of existing research methods and based on current findings. Examples of the coding procedure, transcript sheet and reporting methods are given in the context of a pilot application. It is suggested that the approach is valuable as a conceptual framework which the observer can use to improve his observational skills, provides a sound base for assessment and remediation work and can incorporate new research findings as they become available.

Adult

Bacterial R-bodies with common morphologies and unrolling dynamics are phylogenetically scattered, indicating extensive lateral gene transfer and wide application potential.

Refractile bodies (R-bodies) of gram-negative bacteria are large proteinaceous assemblies, rolled up in the form of an Archimedean spiral. They exhibit rapid rod-like reversible extension in the micrometer range when cued by chemical environmental triggers and have potential for synthetic biology and biochip applications. Initially described for the Paramecium endosymbionts Caedibacter taeniospiralis and Caedimonas varicaedens, R-bodies have since been discovered in many classes of Pseudomonadota, both in endosymbionts and in non-endosymbionts. However, despite the fact that the genetics and morphologies, as well as the unrolling kinetics of R-bodies from different species, show considerable diversity, no recent study has integrated these aspects into a single framework. The latter would be advantageous for the creation of an R-body biotechnology toolbox, where different properties determine the application area. Here, we have examined the R-bodies from six different Pseudomonadota, comprising both phylogenetically diverse endosymbionts and non-endosymbionts. Comparison of the morphologies of the rolled-up and unrolled forms, obtained using electron microscopy and high-quality images, to their corresponding genetic data indicates that extensive lateral gene transfer has occurred, which confounds a common framework based on these data. However, we have also studied the R-body extension and retraction kinetics using high frame-rate light microscopic video recordings, where we show for the first time that R-bodies can be classified into two classes, showing "fast burst" or "slow" acid-induced extension kinetics, respectively. We propose that this criterion may, in fact, be the most useful for the choice of an R-body tool for biotechnological purposes.IMPORTANCER-bodies are unique proteinaceous macromolecular structures capable of massive reversible extension in response to external environmental triggers without the input of chemical energy. They comprise only a few small polypeptides, which makes them potentially highly amenable to tuning via genetic engineering, as well as being exceptionally stable. These properties would be highly desirable in biotechnology and synthetic biology, as well as in biochip applications, where a controlled mechanical extensor might play an integral part in a nanoscale molecular machine. So far, only R-bodies from a single species, Caedibacter taeniospiralis, have been characterized extensively. However, in recent years, genomic information has revealed that a panoply of R-bodies are widely distributed among gram-negative phyla, although studies have generally not included morphological data. This study brings these two areas together to provide a holistic overview of the field and also reveals new insights into key dynamic aspects of R-body extension.

R-bodies

Differences in the velocity-displacement relationship of systolic contraction of normal and ischemic canine myocardium.

In the canine model there exist major differences in the velocity-displacement relationships of systolic contraction between normal and ischemic myocardium. These differences are noninvasively measurable in vivo from video recordings of the fluoroscopic image of the heart, and can be detected within the time period of one cardiac cycle. Velocity data is plotted against the corresponding wall displacement on orthogonal axes (phase-space), producing characteristic loops which give an immediate visual indication of myocardial dysfunction.

Animals

Nonuniform contraction in the isolated cat papillary muscle.

Microspheres infused into the coronary microcirculation were used as markers to define segments within isolated cat papillary muscles. Video recording and analysis provided measurements of the variations of segment lengths as the muscles contracted at lengths of 76-100% Lmax. In all muscles, segments in the center region were found to shorten during muscle isometric contraction while those in the end regions lengthened. Central shortening was typically 10-15%. In the passive state, segment lengths varied directly with muscle length over a broad range characterized by low force. Segments in the center region, however, displayed an abrupt transition to high stiffness at a certain length while end regions continued to stretch. Force-length relationships obtained for the presumably healthy center segment are significantly different from those obtained for the whole muscle. These results suggest that there may be major difficulties with the interpretation of mechanical measurements on papillary muscles unless contractile inhomogeneity is eliminated or taken into account.

Animals

Diagnostic and therapeutic reevaluation of patients with intractable epilepsy.

Intensive monitoring techniques were used to improve seizure diagnosis and control in 23 patients with intractable epilepsy: (1) Video recording of clinical seizures and the ictal EEG, (2) long-term telemetered EEG recordings, and (3) frequent determination of plasma antiepileptic drug concentrations. Patients were monitored in the hospital for an average of 8 weeks and after discharge were followed for an average of 8 months. At follow-up, 70 percent of the patients continued to have improved seizure control as compared with baseline studies, 83 percent had decreased toxicity, and about half had made gains in social adjustment. Use of the techniques described can make a significant contribution to patients previously considered intractable to therapy.

Adolescent

Evaluation of the significance of scintillation angiocardiography for determination of the left ventricular volume.

A method for the determination of the left ventricular volume by scintillation angiocardiography following a peripheral venous injection of a radioactive isotope was described. Salient points in its methodology were as follows: 1. This volume study was composed of non-gated scintillation angiocardiography and cumulative gated scintiphotography. 2. The non-gated scintillation data were stored into video recording system. 3. The gated scintiphotography was performed during a play-back of non-gated scintillation data, opening the gate at desired points of time during a cardiac cycle. 4. The left ventricular scintillation image was photographied in life-size on x-ray film by superimposing, 10-20 times, the gated image of the several consecutive heart beats during the "left ventricular phase" of the dilution of intravenously injected radionuclide. For validation of this method, the stroke volume obtained with this method was compared with that obtained by precordial radionuclide dilution curve recordable during the non-gated scintillation angiocardiography, and also with that obtained by non-simultaneous radiocardiography. It was concluded that the present method may be sufficiently accurate for clinical use.

Adolescent

Infants' reactions to an approaching stranger: description, validation, and functional significance of wariness.

Reliable descriptions of infants' behavioral responses to an approaching stranger were made from video records. Subtle negative responses (wariness) were validated against heart rate acceleration and responses to mother approach and showed significant age changes. Behavioral aspects of wariness appear to serve a "cutoff" (coping) function for the infant, preventing all-or-none responses (crying) and facilitating subsequent reengagement of the stranger. Attention to both positive and negative responses, especially in the milder forms, helps clarify inconsistencies in reported age of onset and frequency of "stranger fear." The role of data on infants' responses to strangers in formulating an integrated picture of development in the second half-year of life is discussed.

Age Factors

[Further developments of the AMP-system. A report on the 2nd AMP-symposium and training seminar (author's transl)].

This is a report of a symposium and training seminar of the "Arbeitsgemeinschaft für Methodik und Dokumentation in der Psychiatrie" (AMP). Video-recordings of two psychiatric interviews were shown during the seminar and rated by the participants using the AMP-3-Scale (Psychopathology). Aim of the seminar was a rater training and the preparation of an expert standard rating. These videotapes with the corresponding standard ratings will enable the different clinics using the AMP-System to compare their rating behaviour. The process of achieving an expert standard rating is described. The training seminar was followed by a symposium during which a series of lectures was presented on "Standardized Assessment in Psychiatry", The different working groups of the Association reported about their work and future prospects of the Association were discussed.

Congresses as Topic

Electronic radiography in neurosurgery.

The authors describe the use of electronic radiography or short-exposure fluoroscopy with concurrent video recording of images in neurosurgery. The technique is applicable to all neurosurgical procedures in which fluoroscopy is used, such as transphenoidal surgery, trigeminal RF rhizolysis, percutaneous cordotomy, etc. With the use of subtraction techniques and intra-operative angiography, vascular maps of the cerebral vascular system can be made that allow the surgeon to puncture or to avoid vascular structures during stereotaxic procedures. Besides a great reduction in radiation exposure, this technique offers new advantages and potential for neurosurgical and neuroradiological interventional procedures.

Cerebral Angiography

[Repeated sampling of rat polymorphonuclear leucocytes from the pleural cavity. Application for the study of leucocyte chemotaxis (author's transl)].

A method is described for allowing the sampling of a homogeneous population of polymorphonuclear cells (PMN) from the rat pleural cavity a well as a technique involving the use of a phase-contrast microscope and video-recording system to study the necrotaxis of rat PMN towards a single erythrocyte lysed by ruby-laser irradiation. This allows both the speed of the leucocytes and the degree of their chemotactic response to be determined. The advantages and disadvantages of the experimental technique are discussed along with its application to the study of in vitro and in vivo actions of various drugs on rat PMN chemotaxis.

Animals

[Repeated sampling of rat polymorphonuclear leucocytes from the pleural cavity. Application for the study of leucocyte chemotaxis (author's transl)].

A method is described for allowing the sampling of a homogeneous population of polymorphonuclear cells (PMN) from the rat pleural cavity a well as a technique involving the use of a phase-contrast microscope and video-recording system to study the necrotaxis of rat PMN towards a single erythrocyte lysed by ruby-laser irradiation. This allows both the speed of the leucocytes and the degree of their chemotactic response to be determined. The advantages and disadvantages of the experimental technique are discussed along with its application to the study of in vitro and in vivo actions of various drugs on rat PMN chemotaxis.

Animals

Gaze patterns of mentally retarded adults in two contrasting environments.

Two matched samples of mentally retarded adults were video recorded on two occasions, before and after one group was transferred to a different type of hospital, and their gaze patterns analyzed. Overall, only slight changes after transfer were found, but analysis according to amount of looking at the interviewer on the first occasion showed that those transferred subjects who initially looked little of the time significantly increased their amount of looking after transfer. Some correlations with the Adaptive Behavior Scale were also reported.

Adult

[Scientific fundamentals of hippo- and riding therapy - a compilation of study results (author's transl)].

The article surveys some of the scientific fundamentals of hippo- and riding therapy, and its methods using the characteristic horseback three dimensional rhythmic movement impulses for its therapeutic values, particularly in cerebral palsy. The influence of riding on posture and balance reactions, position of the pelvic, postures of head and trunk as well as rotatory movements was demonstrated in film studies using the cerebral palsied with different syndromes as an example. Electromyographic examinations of antagonistic muscle groups, kinesiologic analyses with the help of a video-recorder, as well as motor tests to evaluate coordination and balance control confirm the practical experience, i.e., that the hippo- and riding therapy has a positive influence on the neuromuscular dysfunction in cerebral movement disorders. A lasting improvement of motor and intellectual abilities was proven by a long-term study which also revealed that motivation was the driving force.

Animals

[Video technic in the hospital--play or progress?].

Many surgical departments indlcuding the ENT field have installed video recording systems or simple b/w or color TV camera and monitors. Using a TV color system has been quite successful for ENT purposes in the operating room. The camera is used in microsurgical work (tympanoplasty etc.) and is connected to the microscope so that the picture can be controlled, corrected, analysed and explained by the teacher. Recording systems are presented and the "utopic" question of compatibility is discussed.

Documentation

Microvascular plasma velocity and indicator dispersion with hemorrhage.

The effects of stepwise hemorrhages on plasma flow velocity and indicator (FITC-Dextran) dispersion in series and parallel coupled microvascular vessels has been studied. The mesentery of cats (0.6 kg) anesthetized with Dial-Urethane was exposed and studied with a microscope equipped for fluorescense microscopy. Indicator was injected as a bolus (0.1 ml in 0.5 seconds) in a small branch of the mesenteric artery. Indicator curves were recorded from a video tape recording of the passage of the indicator by a video sampler with intensity sensitive windows. The mean transit time (t), appearance time (t(a)), peak time (t(p)), curve duration (t(E)), plasma velocity and vessel diameter changes were determined. Arterioles 68 +/- 8 micron and 35 +/- 3 micron and venules 55 +/- 6 micron constricted gradually with hemorrhage. Arterioles 20 +/- 2 micron constricted with mild hemorrhage and remained constricted with further hemorrhage. Arteriolar and venular plasma velocities decreased abruptly with mild hemorrhage and then continued to decrease gradually with further hemorrhage. Capillary flow ceased after moderate hemorrhage and was not reestablished until at least an hour after reinfusion. Arteriole t increased with hemorrhage but t increased much more in capillaries and venules. t(E) changes paralled t changes. The ratio t(a)/t was lower for venules than arterioles and both decreased with hemorrhage but the venular ratio decreased significantly more. Venular t(p)-t(a) and t(E)-t(p) increased more than arteriolar values. Hemorrhage increased indicator dispersion with the greatest effect occurring between the arteriolar and venular vessels.

Animals

Identity of emotional triggers in epilepsy.

This communication describes a technique of stimulated recall and video replay which has reduced the frequency of seizures in five epileptic patients. Each of the patients had long standing partial epilepsy with complex symptomatology of the psychomotor type. It was generally acknowledged that emotional factors played an important role in their poor seizure control. Previous psychotherapeutic efforts had been without benefit because ictal amnesia had erased the memory of the stressful antecedent message-input which had triggered the seizures. Creation of empathetically stressful responses to presentation of audio and video tape recordings of specific problematic social interactions was sufficient to induce seizures in these patients. Video tape recording of the seizure and the antecedant events provided by means by which the patients could acquire otherwise unrecognized or forgotten information. Once equipped with the identity of the specific emotional trigger, the patient could avoid the kinds of events which might be expected to induce a seizure and be better able to cope with threatening environmental cues when encountered in the future.

Adult