SEARCH · PubMed Health
Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.
Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.
Omiderm, a synthetic temporary skin substitute, was used for the treatment of skin defects in a newborn who suffered from epidermolysis bullosa. Omiderm served as a biological barrier, and its transparency allowed close observation of the wound healing process and early detection of local infections when they occurred.
Explore the source record for details and available documents.
Biological skin is effective in restoring the missing water vapor barrier and promoting healing in burn wounds. Its effectiveness in wound management has been limited, however, by its inherently limited antibacterial properties and the fact that it is sometimes rejected before healing is complete, even reversing previous beneficial effects. Limited availability and storage difficulties have posed further problems. Impregnation of biological skin with silver ions has been proven to provide a potent bactericidal effect directly at the wound surface. We hypothesized that aldehyde cross-linking of silver-impregnated skin would mask the histocompatibility sites from the recipient's immune system. This has been demonstrated previously with aldehyde cross-linking of allografts and xenografts, prolonging retention sufficiently to permit complete wound healing. Commercially available skin was treated with an aldehyde compound and impregnated with silver. Initial studies of this cross-linked skin for treatment of burn wounds showed average retention to be between 117 and 161 days, far exceeding that of any untreated skin. It was subsequently found that the aldehyde cross-linking permitted impregnation with higher concentrations of silver than had previously been possible--2,600 to 2,830 ppm as compared to an average of 1,020 to 1,350 ppm in previously available silver-impregnated skin. This results in a more potent, immediate antibacterial effect at the wound surface and an extended period of time-release antibacterial action before the silver is exhausted. The antibacterial properties of this aldehyde cross-linked silver-impregnated skin are effective in decontaminating even grossly infected wounds and in protecting against contamination of clean wounds from adjacent infected areas or external sources.(ABSTRACT TRUNCATED AT 250 WORDS)
Hypodense liver lesions with attenuation coefficients indicative of fat density were seen on CT of three patients followed up for suspected metastatic spread of gastrointestinal malignancy. Knowledge of previous hepatic surgery using omental packing for hemostasis in the setting of liver trauma or lobectomy was useful in preventing a false-positive interpretation of postoperative complications.
Neonatal Fischer 344 rat keratinocytes were cultured on a collagen sheet to create a 'quasi-skin.' The cells were grown in either control medium or a low-calcium supplemented medium to enhance proliferation. The rate of 3H-thymidine incorporation into DNA was assessed at days 3, 7, 10, and 20 to determine the effectiveness of the medium supplements. The cells grown in supplemented medium did have an elevated rate of incorporation. On days 7 and 14 post culture, the quasi-skin was used to cover full-thickness defects on the dorsum of adult male rats. The covered defects were biopsied 45 and 60 days postoperatively. Light microscopy showed the wounds to be completely re-epithelialized. Barr bodies were present at the wound site, confirming the presence of quasi-skin keratinocytes up to 60 days postoperatively.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
A chemically treated bovine skin, Dermodress, is presented as a long-term skin substitute. Dermodress was applied over denuded surfaces of debrided deep burns and was kept in place for up to 56 days. Dermodress adheres to all contours of the debrided surfaces, has hemostatic effect and does not present any signs of infection or rejection. Dermodress can be removed in stages according to the availability of autografts, which may be applied immediately, since the exposed areas are clean and suitable for immediate grafting. Our clinical experience shows that Dermodress has most of the required properties of a long-term temporary skin substitute.
Explore the source record for details and available documents.
Compression is still the cornerstone of treatment for these common wounds. Learn how to choose the right product for your patient.
Cultured keratinocytes were used as allografts to treat 51 patients with chronic venous ulceration or rheumatoid ulcers unresponsive to all previous conventional treatments including split skin grafts. Although early epithelialization could be seen in the centre of some ulcers, a major effect appeared to be healing from the previously indolent edge. This treatment appears to provide some clinical benefit in healing of chronic ulceration.
Palliative treatment of an enterovaginal fistula secondary to adenocarcinoma of the rectum in a 65-year-old woman with intraperitoneal corium porcine graft is reported. The patient had previously had a total colectomy for ulcerative colitis followed by excision of the rectal stump, mucosal proctectomy and ileostomy.
Collagen membrane preparations have been manufactured with the aim of enhancing wound healing following periodontal surgery. After cross-linking by various processing methods (with ultraviolet radiation or hexamethylenediisocyanate) and to various extents, atelocollagen membranes were applied into dissection sites within palatal gingival tissue. Applied atelocollagen was histopathologically compared with applied lyophilized porcine dermis (LPD) and controls in rats, with regard to the time course of healing. The atelocollagen-applied group showed more satisfactory regeneration of the epithelium and connective tissue in an artificially created gingival defect than did the control group or the LPD-applied group. Epithelial downgrowth along the root surface was significantly suppressed by the use of atelocollagen. In addition, the post-operative inflammatory reaction and foreign body giant cell reaction subsided rapidly after surgery in the atelocollgen-applied group. Our results show that the use of atelocollagen membrane in periodontal wounds should be the method of choice.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
To determine the survival of cultured allogeneic keratinocytes transplanted to a deep dermal bed 24 tattoos that had been removed by deep shave excision in 19 patients were grafted with sheets of cultured allogeneic keratinocytes from donors of the opposite sex. Cells carrying the Y chromosome were identified in biopsy specimens taken from the graft site by in situ DNA hybridisation with a biotinylated Y probe (pHY 2.1) and visualised with a technique using immunoperoxidase. The cultured allograft sites were biopsied one, two, and three weeks after transplantation. No male cells were identified in any biopsy specimen from female patients who were given transplants of male cultured keratinocytes, and all biopsy specimens from male patients, who received female cultured keratinocytes, showed percentages of male cells within the normal range for male skin. The beneficial effects of cultivated allogeneic keratinocytes result from effects on wound healing other than forming a successful graft that "takes."
Explore the source record for details and available documents.