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At least 541 records · Page 30Linked to original sources
PubMed1994

Thoracoscopic sympathicotomy for arterial insufficiency.

Arterial insufficiency of the hands due to vascular spasm-morbus Raynaud is an extremely unpleasant condition. Elicited by cold temperatures, the disease can make it impossible for the patient to go out in cold environments. The condition seldom leads to skin necrosis, which is the case in arterial insufficiency caused by vascular occlusion. In both cases arterial insufficiency may be diminished by thoracic sympathectomy. Our test series of operations included 14 patients with m. Raynaud or vascular occlusion. All experienced improvement, with warm, dry hands and satisfactory healing after thoracoscopic sympathicotomy. However, after six months, the original symptoms recurred in all the patients with m. Raynaud, while the improvement continued in the patients with vascular occlusion during the two to four year period of observation.

Arterial Occlusive Diseases↗
PubMed1992

Temperature sensitivity of vinblastine-induced tubulin polymerization in the presence of microtubule-associated proteins.

The antitumor drug vinblastine has been a useful probe for examining the interaction of tubulin with the microtubule-associated proteins (MAPs), specifically with tau and MAP 2. Although tau and MAP 2 can stimulate microtubule assembly in vitro, their specific interactions with tubulin are known to differ. For example, in the presence of vinblastine, both tau and MAP 2 cause tubulin to form spirals, but tau causes formation of clustered spirals of high turbidity, while MAP 2 causes formation of loose spirals of low turbidity [Ludueña et al., J. Biol. Chem. 259, 12890-12898 (1984)]. Although cold temperatures can inhibit microtubule assembly, cold has no effect on vinblastine-induced tubulin spiral formation. Consequently, we used the vinblastine-tubulin system to examine the interactions of tau and MAP 2 with tubulin at low temperatures. We found that tau-tubulin-vinblastine complexes form about as well at 0 degree C as at 37 degrees C. In contrast, MAP 2-tubulin-vinblastine complexes form much less well at 0 degrees C than at 37 degrees C. We find, however, that MAP 2, at 0 degrees C, will strongly inhibit, and even reverse, formation of the tau-tubulin-vinblastine complex. This suggests that the temperature-sensitive factor is the MAP 2-stimulated tubulin-tubulin interaction rather than the MAP 2-tubulin interaction per se; this raises the possibility that the tubulin-tubulin interactions stimulated by tau differ in their temperature sensitivity from those stimulated by MAP 2.

Animals↗
PubMed1998

Post exercise changes in compartmental body temperature accompanying intermittent cold water cooling in the hyperthermic horse.

Whereas the efficacy of cold water cooling of horses has been demonstrated by several studies, the dynamics of temperature changes within and between compartments (primarily muscle, blood [core], skin and deep core [rectal]) have not been investigated. Changes in body temperature associated with cold water cooling were investigated in the hyperthermic horse. Muscle (TMU), pulmonary artery (TPA), rectal (TREC), tail-skin (TTSK) and coat surface (TCOAT) temperatures, were monitored continuously in 5 Thoroughbred horses during and after exercise in hot humid (30 degrees C and 80% RH) conditions on a treadmill. Horses were cooled in the hot humid environment with cold water (approximately 6 degrees C) for 6 30 s periods. Between each 30 s cooling period the horses stood for 30 s. A total of 180 l of cold water was applied. Horses were monitored for a further 4 min following the final cooling period. From the end of exercise to the end of the final cooling (6.5 min), mean (+/- s.e.) rates of decrease for TTSK and TPA were similar (0.8 +/- 0.1 and 0.8 +/- 0.1 degrees C/min, respectively). The effects on TMU and TREC were less marked, with average rates of 0.2 +/- 0.1 and 0.0 +/- 0.1 degrees C/min, respectively. During the first 4 min of cooling, TPA fell during the 30 s period of water application and rose during each 30 s period of standing. When TPA fell below approximately 36.5 degrees C, these variations were suppressed and TPA rose steadily, despite continued applications; TREC and TMU continued to fall, although less rapidly than before. These observations are consistent with the onset of skin vasoconstriction at low TPA. The mechanism is mediated through a cooling of circulating blood volume providing a greater capacity for heat transfer between muscle and circulation. Intermittent application of cold water (approximately 6 degrees C) improves heat removal without apparent deleterious effects and is well tolerated. Even when hypothermia develops (based on TPA), muscle and rectal temperatures continue to fall.

Animals↗
PubMed1998

Daily mortality in Madrid community 1986-1992: relationship with meteorological variables.

Daily mortality displays a seasonal pattern linked to weather, air pollution, photoperiod length, influenza incidence and diet, among which temperature ranks as a leading cause. This study thus sought to assess the relationship between temperature, relative humidity, wind speed and mortality in the Madrid Autonomous Region (Spain) for the period January 1986-December 1992, controlling for the effects of air pollution and influenza incidence. Daily data on maximum, minimum and 24-hour mean temperature, relative humidity and wind speed were matched against daily mortality. Transfer function was identified using the Box-Jenkins pre-whitening method. Multivariate time series regression models were used to control for the confounding effects of air pollution and influenza incidence. Separate seasonal analyses were carried out for winter and summer periods. A J-shaped relationship between outdoor temperature, relative humidity and daily mortality was found. Mortality proved to be inversely related to cold temperature (4- to 11-day lag) and directly related to warm temperature (1-day lag). High relative humidity during summer periods was negatively related to mortality. Thermal variation ascribable to Madrid's mesothermal Mediterranean climate was strongly related to daily mortality, even where air pollution and influenza incidence were controlled for.

Age Distribution↗
PubMed1978

Relation of hormonal variations to nutritional studies and metabolism of ruminants.

Variations in concentrations of growth hormone, insulin, glucagon, prolactin, adrenal corticoids, and thyroid hormones in blood plasma of ruminants as related to circadian rhythms, environmental factors and nutrition are reviewed. Rhythms in prolactin concentrations are related to light and temperature. Concentrations of prolactin in plasma are higher in warm environments and during longer photoperiods. There is episodic secretion of growth hormone in cattle with frequent peaks in plasma concentrations during 24 h. Marked increases in environmental temperature result in greater secretion of growth hormone for short times followed by a decline in plasma concentration with prolonged exposure. Extreme cold temperature also increases growth hormone concentrations in plasma. Secretion of thyroid hormones increases in cold and decreases in warm environments. Circadian rhythms are definite for plasma concentrations of adrenal corticoids in ruminants adjusted to their surroundings. Peaks occur late in dark periods, and lows occur during the end of light periods. Circadian patterns have not been consistent for secretion of insulin. Prolonged subjection to stresses such as noise, handling, and restraint can modify concentrations of prolactin, growth hormone, and adrenal corticoids. Changes in concentrations of insulin may occur if blood glucose is increased. After feeding, concentrations of growth hormone in plasma decrease and concentrations of insulin and glucagon increase. Relationships of these changes with metabolism are discussed.

Adrenal Cortex Hormones↗
PubMed1988

Influence of sarcoptic mange and cold and ambient temperature on blastogenic responses of lymphocytes and serum cortisol concentrations of pigs.

Blood samples from sarcoptic mite-infested pigs were evaluated for effects of mite infestation and cold and ambient temperatures on lymphocyte blastogenic responses and for effects of mite infestation on serum cortisol concentrations. In experiment 1, sarcoptic mite-infested and noninfested pigs were housed in cold (5 to 15 C fluctuating) and thermoneural (25 C) environmental chambers for 5 weeks. Differences were not observed (P greater than 0.10) in blastogenic responses to phytohemagglutin or pokeweed mitogen between lymphocytes from infested and noninfested pigs on postinfestation days (PID) 7, 21, 28, and 35 in either environmental chamber. When lymphocytes from noninfested pigs were cultured with sera from infested pigs, alterations of blastogenic responses were not detected. Cortisol values were higher (P less than 0.05) in sera from sarcoptic mite-infested pigs, compared with those from noninfested pigs, at 4 PM on PID 14 and 4 AM and 10 AM on PID 15. Cortisol values were higher (P less than 0.05) in sera obtained at 10 AM on PID 14 and at 10 AM on PID 15 from pigs housed in cold chambers, compared with those from pigs housed in thermoneutral chambers. Interactive effects between sarcoptic mite infestation and cold ambient temperatures were not observed. At 4 AM on PID 15 (experiment 2), cortisol values were higher (P less than 0.05) in sera of infested pigs, compared with those in noninfested pigs. Seemingly, sarcoptic mange in pigs did not alter mitogen-induced lymphocyte blastogenic responses, but did increase serum cortisol concentrations, indicating that sarcoptic mange may be a stressor in pigs.

Animals↗
PubMed1985

Human brain tubulin purification: decrease in soluble tubulin with age.

The soluble tubulin of human cerebral cortex, as assessed by [3H]colchicine binding of the 100,000 g supernatant fraction, decreases drastically with age, 75 percent from age 0 to age 90. There is also a considerably lower concentration of high molecular weight proteins in the soluble fraction of postmortem human cerebral cortex than in that of nonhuman species. Human brain tubulin can be polymerized into microtubules with DEAE-dextran. The DEAE-dextran induced microtubules are stable to cold temperature (4 degrees) and calcium. However, in the presence of 1 M glutamate, the microtubules become cold labile and depolymerize at 4 degrees. Thus we have developed a novel method for purifying polymerization competent tubulin from fresh or frozen human cerebral cortex. Human brain tubulin purified by our novel method is very similar to tubulin from the brains of other mammals in molecular weight, amino acid composition, polymerization-depolymerization parameters, and structural dimensions of the microtubules formed.

Adolescent↗
PubMed1989

Environmental temperature and mortality from acute myocardial infarction.

Mortality from acute myocardial infarction (MI) over the 5 year period 1982-1987 in Brown County, Wisconsin, was analyzed to assess the relationship with environmental temperature. Deaths occurring on the day of and the day following a significant snowfall as well as deaths occurring in health care facilities were eliminated from consideration because the focus was upon temperature, not snowfall or events within a hospital. These criteria resulted in the inclusion of 1,802 days and 926 cases of acute MI. The mean temperature on the day of death was obtained from climatological data and were grouped into six categories covering a range of temperatures from less than -17.8 degrees C (0 degrees F) to 16.1 degrees C (61 degrees F). The number of deaths in each category was tabulated. The effect of temperature, sex, and age were analyzed by regression analysis. The results indicated a linear increase in mortality as mean daily temperature decreased over the temperature range. The inverse temperature effect was most pronounced in males over the age of 60. These results indicate that cold temperatures appear to be associated with an increased mortality from myocardial infarction.

Aged↗
PubMed1992

Effects of Temperature on Two Psychrophilic Ecotypes of a Heterotrophic Nanoflagellate, Paraphysomonas imperforata.

Two different psychrophilic types of the heterotrophic nanoflagellate Paraphysomonas imperforata were isolated from Newfoundland coastal waters and the Arctic Ocean. When fed bacteria without food limitation, both isolates were able to grow at temperatures from -1.8 to 20 degrees C, with maximum growth rates of 3.28 day at 15 degrees C and 2.28 day at 12.3 degrees C for the Newfoundland and the Arctic isolates, respectively. Ingestion rates increased with temperature from 14 to 62 bacteria flagellate h for the Newfoundland isolate and from 30 to 99 bacteria flagellate h for the Arctic isolate. While temperature did not affect cell yields (number of protozoa produced divided by number of bacteria consumed), it affected flagellate sizes. This differential effect of temperature on cell yield and cell size resulted in a changing gross growth efficiency (GGE) in terms of biovolume; colder temperatures favored higher GGEs. The comparison of Q(10) values for growth rates and ingestion rates between the isolates shows that the Arctic isolate is better adapted to extremely cold temperature than the Newfoundland isolate. At seawater-freezing temperature (-1.8 degrees C), the estimated maximum growth rates and maximum ingestion rates are 0.81 day and 30 bacteria flagellate h for the Arctic isolate and 0.54 day and 12 bacteria flagellate h for the Newfoundland isolate. Our findings about psychrophilic nanoflagellates fit the general characteristics of cold-water-dwelling organisms: reduced physiological rates and higher GGEs at lower temperatures. Because of the large and persistent differences between the isolates, we conclude that they are ecotypes adapted to specific environmental conditions.

Journal Article↗
PubMed2001

Serum concentrations of collagen degrading enzymes and their inhibitors after downhill running.

In the present study the release of proteins degrading extracellular matrix compounds to circulation was measured after damaging exercise in humans. Muscle damage was induced by downhill running; furthermore, the exercise was performed at both cold temperature (5 degrees C) and room temperature (22 degrees C) to study also the possible effect of environmental temperature on serum concentrations of matrix metalloproteinases MMP-2 and MMP-9, tissue inhibitors of metalloproteinases TIMP-1 and TIMP-2, and MMP-2/TIMP-2 complex, and muscle damage monitored by serum creatine kinase measurements. Results were compared with those obtained from patients having rhabdomyolysis, myositis and Becker muscular dystrophy. The present study demonstrates an acute increase in serum concentrations of MMP-9, TIMP-1, and MMP-2/TIMP-2 complex, but no changes in serum MMP-2 concentrations in response to eccentric exercise. Serum creatine kinase activity data suggest greater muscle damage after downhill running in a cold environment than at room temperature. The present observations about at most slight changes in serum MMP and TIMP concentrations and lack of their correlation to increased serum creatine kinase after exercise indicate that serum measurements of MMPs and TIMPs do not sensitively respond to exercise induced skeletal muscle damage and extracellular matrix regeneration. On the other hand, severe skeletal muscle damage, such as rhabdomyolysis, myositis and Becker muscular dystrophy, seemed to have an effect on serum MMP and TIMP concentrations.

Adult↗
PubMed1991

[Temperature profile in the enamel during cold tests].

The temperatures on the surface of human teeth during pulp vitality tests with frigen and carbon dioxide snow were recorded in an in vitro study. Frigen spray caused particularly rapid changes in temperature. The lowest temperature (-52 degrees C) was measured with CO2-snow (with a pressure of 9.81 N against the tooth surface). To obtain higher temperatures at lower rates of change, the use of a thin latex fingerstall proved to be effective.

Body Temperature↗
PubMed1991

Folding intermediates of hyperthermophilic D-glyceraldehyde-3-phosphate dehydrogenase from Thermotoga maritima are trapped at low temperature.

D-Glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic eubacterium, Thermotoga maritima, is extremely thermostable showing a thermal transition beyond 105 degrees C. At low temperature, 'cold denaturation' becomes detectable only in the presence of destabilizing agents. Reconstitution after preceding denaturation depends on temperature. At 0 degree C, no significant recovery of activity is detectable, whereas between 30 and 100 degrees C reactivation reaches up to 85%. Shifting the temperature from low values to the range of optimum reconstitution releases the trapped intermediate in a fast reaction. Evidence from ultra-centrifugal analysis and far-UV circular dichroism proves the intermediate to be partially assembled to the tetramer, with most of its native secondary structure restored in a fast reaction. Fluorescence emission exhibits at least biphasic kinetics with the rate-limiting step(s) reflecting local adjustments of aromatic residues involved in tertiary contacts in the native state of the enzyme.

Glyceraldehyde-3-Phosphate Dehydrogenases↗
PubMed2003

Effect of ambient temperatures ranging from cold to heat on thermoregulation in conscious MK801-treated rats.

The glutamate NMDA receptor has been suggested to be involved in thermoregulation. To further analyse its role, the thermoregulatory responses of rats treated with 0.5 mg.kg-1 of dizocilpine (MK801) were compared with those of control rats treated only with the same volume of saline during a 180-min exposure at one of the six different ambient temperatures, ranging from cold to heat. Colonic temperature (Tco) and tail skin temperature (Ttail) were measured throughout using Cu-Ct thermocouples. In the cold (2.4 and 12.3 degrees C), Tco decreased either sharply (MK801) or progressively (saline), reaching the same final value (2.4 degrees C) or a lower value in the MK801-treated rats (12.3 degrees C). At the same time, Ttail decreased in both groups. In the cool environment (20.7 degrees C), Tco and Ttail decreased in both groups, with lower final values in MK801-treated rats. At thermoneutrality (28.8 degrees C), the MK801-induced hyperthermia remained steady, while Ttail increased in both groups. In the heat (34.6 and 36.2 degrees C), Tco and Ttail increased in both groups, with higher final values in MK801-treated rats. Moreover, at 36.2 degrees C, only MK801-treated rats exhibited heatstroke. It is thus suggested that MK801-induced inhibition of NMDA receptors impairs thermoregulation, especially in the heat.

Animals↗
PubMed1977

A comparison of the uptake of [1-14C]palmitate into cell fractions of the perfused toad, chick and guinea-pig heart.

1. The incorporation and washout of [1-14C]palmitate in the subcellular fractions of toad, chick and guinea-pig hearts has been studied at warm and cold temperatures. 2. Maximal FFA radioactivity generally occurred in the microsomal fraction. Washout of radioactivity in chick and guinea-pig hearts virtually ceased in the cold, while in the toad it was only moderately affected. 3. The microsomal esterification process was temperature dependent. It was most active in the guinea-pig and least active in the toad.

Animals↗