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Simulated high altitude diving experiment for the underwater construction operation.

The simulated dive experiments were conducted at the high altitude of 4500 meters and 5000 meters, for the requirement of diving operation in the lakes at the altitude of 4442 meters for the construction of large-scale hydroelectric power station. The high & low pressure chamber-complex was used, and 15 professional divers participated in the experiment. The divers were stayed at the altitude of 4500 and 5000 meters for 7-9 days. Totally 85 persons-times of dives to the depths of 30-50 meters were operated; they stayed under the water for 30-90 minutes while processing physical activities. During the experiment, we studied the pressurization procedure, decompression table, and physiological functions of the divers. The results indicate that, although the relative pressure differences between the surface and underwater was larger at high altitude than at sea level, the appropriate prolongation of the compression time was able to prevent the difficulty in pressure regulation for the divers to avoid the injury of middle ear. Four tables of the decompression A, B, C and D was calculated with Haldane's theory, and the speed of decompression increased in the order from A to D. The safest procedure was C, and there was no decompression sickness and bubbles in body of the divers. The methods of decompression included underwater stage decompression, surface decompression, oxygen-breathing decompression, and repetitive diving decompression. The surface decompression was the most suitable method for the high altitude, as it could greatly decrease the time in the cold water for the divers. The power spectrum analysis of EEG (electroencephalogram) indicated that, when the divers were exposed to the altitude of 5000 meters, the delta activity in EEG increased, alpha and beta activity decreased. And the delta activity decreased, the alpha and beta activity increased while diving during a dry condition. According to the diving and decompression procedure studied under simulated conditions, 272 person-times of diving training and underwater operations were processed in a high altitude hydroelectric power station at the altitude of 4442 meters, including photographing, video-recording, measuring, and drilling. There were no signs and symptoms of decompression sickness and bubbles.

Adaptation, Physiological↗

Aggregation of killer whale platelets.

The aggregation of blood platelets is a crucial step in normal hemostasis for all mammals. Circulating platelets are sensitive to a large variety of physiologic and non-physiologic stimulants, some of which are formed or exposed in conjunction with vascular damage or endothelial cell denudation. In addition, drastic pressure changes activate human platelets. Killer whale platelet function, on the other hand, is very intriguing since these animals do not seem to experience untoward platelet reactions during or after diving to great depths, nor do they experience abnormal bleeding associated with sub optimal platelet function. We examined this concept and determined that killer whale platelets, in response to ADP, PAF, and arachidonic acid, appeared to aggregate normally during the first 2-5 minutes after addition of the agonist, but had completely disaggregated at 10 minutes. Collagen- and A23187-induced aggregation appeared normal and complete within 10 minutes, while there was no response to epinephrine or ristocetin. Thromboxane production by killer whale platelets appears to be quantitatively similar to that produced by human platelets in response to ADP and PAF and exceeded that produced by human platelets when collagen was used as the agonist. In summary, this study reports a reduced platelet aggregation reaction in killer whales in response to several platelet agonists which does not appear to be related to the generation of thromboxane. This phenomenon may serve a protective role in these mammals by preventing thrombosis during diving and resurfacing.

Adaptation, Physiological↗

The myoglobin of Emperor penguin (Aptenodytes forsteri): amino acid sequence and functional adaptation to extreme conditions.

In the framework of a study on molecular adaptations of the oxygen-transport and storage systems to extreme conditions in Antarctic marine organisms, we have investigated the structure/function relationship in Emperor penguin (Aptenodytes forsteri) myoglobin, in search of correlation with the bird life style. In contrast with previous reports, the revised amino acid sequence contains one additional residue and 15 differences. The oxygen-binding parameters seem well adapted to the diving behaviour of the penguin and to the environmental conditions of the Antarctic habitat. Addition of lactate has no major effect on myoglobin oxygenation over a large temperature range. Therefore, metabolic acidosis does not impair myoglobin function under conditions of prolonged physical effort, such as diving.

Adaptation, Physiological↗

Technique for electrocardiographic monitoring of working divers.

An improved technique to record high-equality electrocardiographic (ECG) signals on the surface, from immersed humans during rest and exercise, in both normothermic and hypothermic exposures, has been devised. Recorded tracings were adequate for research purposes. Waveform signals obtained allow accurate continuous monitoring as well. The best recordings resulted from proper selection of electrode placement sites, careful preparation of the skin, and diligent protection of the skin-electrode interface. The resulting signals recorded from male divers working in 3.05 m (10 ft) of water contained very little artifact or shift in baseline of the ECG tracing. Post-dive examination of the placement sites showed neither separation of ECG electrodes from the skin nor intrusion of water between the electrode and skin surface. The relationship of the new ECG recording technique to a previously described system for acquiring physiologic data is described.

Diving↗

Immune responses in humans after 60 days of confinement.

A confinement experiment in a normobaric diving chamber was undertaken to better understand the effect of confinement and isolation on human psychology and physiology. Pre- and postconfinement blood samples were obtained from four test subjects and control donors to analyze immune responses. No modification in the levels of CD2+, CD3+, CD4+, CD8+, CD19+, and CD56+ cells was observed after confinement. Mitogen-induced T-lymphocyte proliferation and interleukin-2 receptor expression were not altered significantly. Whole blood interferon-alpha and gamma-induction and plasma cortisol levels were also unchanged, as was natural killer cell activity. These data suggest that in humans, no specific components of the immune response are affected by a 2-month isolation and confinement of a small group.

Adult↗

Immunohistochemical fiber typing of harbor seal skeletal muscle.

There is strong evidence that pinnipeds maintain a lipid-based, aerobic metabolism during diving. However, the few fiber-typing studies performed on pinniped skeletal muscles are not consistent with an aerobic physiological profile. The objective of this study was to reexamine the fiber type distribution throughout the primary locomotory muscles of the harbor seal Phoca vitulina. Results from immunohistochemical (IHC) fiber typing indicated that harbor seal swimming muscles (the epaxial muscles) are composed of 47.4% type I (slow twitch, oxidative) fibers and 52.8% IIa (fast twitch, oxidative) fibers, which are homogeneously distributed throughout the muscle. Harbor seal pectoralis, a secondary swimming muscle, was composed of 16.2% type I and 84.3% type IIa fibers. No fast twitch, glycolytic (type IIb) fibers were detected in either muscle, in contrast to published data on fiber typing of harbor seal epaxial muscles using traditional histochemical techniques. The extreme specificity inherent in the IHC fiber typing procedure leads us to conclude that harbor seal swimming muscle is entirely composed of oxidative fibers. Our results are consistent with the enzymatic analyses of pinniped skeletal muscle that support the use of lipid-derived aerobic catabolism to fuel working muscle during diving in these marine mammals.

Analysis of Variance↗

Unconventional ventral attachment of time-depth recorders as a new method for investigating time budget and diving behaviour of seabirds.

We tested the use of commercially available electronic time-depth recorders (TDRs) to quantify activities and thus total time budgets of seabirds. This new method involved first fitting TDRs onto the birds' bellies (not on their backs), and, secondly, analysing continuous recordings of temperature, light and pressure to differentiate activities on land and at sea. The birds studied were 12 common guillemots (Uria aalge) rearing chicks at Hornøya, in northern Norway. The method successfully recorded five different activities: at the colony, flying, diving, and resting or active at the sea surface. Overall, common guillemots spent 68% of their time at the colony and 32% at sea. While at sea, the birds spent the majority (77%) of their time at the surface, during which they were active 64% of the time, and rested only 13%. Birds engaged in the costly behaviours of flying and diving for shorter times (11% and 12% of their time at sea, respectively). The method allowed us to differentiate between two types of trips to sea based on the presence (foraging trips: 77% of the total number of trips) or absence (non-foraging trips: 23%) of dives. On average, foraging trips lasted 3.2 h, but most trips were shorter (<1 h), during which the mean estimated travel distance from the colony was 11 km. Diving occurred in bouts of 7.7+/-6.6 dives (mean +/- S.D.). The mean maximum dive depth was 10.2+/-7.6 m (deepest dive: 37 m), and the mean dive duration and post-dive intervals were 38.7+/-21.3 s (longest dive: 119 s) and 20+/-12 s, respectively. Direct and indirect evidence suggests that common guillemots had no difficulty in finding food during the study period, and that the TDRs had minimal effects on the birds' behaviour and physiology. The method is easy to use in the field and is applicable to many other flying seabird species; it is therefore an efficient way of collecting information on time budgets and diving behaviour in the context of various ecological and monitoring studies.

Animals↗

[Aviation physiology].

Aviation physiology should be known at least in parts by the physicians advising air travellers. Due to reducing atmospheric pressure at altitude gas volume in body cavities expands (Boyle's law). This might not be a problem during ascend since air can disappear easily through natural ways. However, air must return to body cavities during descend and a person with a cold may suffer from painful barotitis. Hypoxia is mostly due to a reduced pO2 in high altitude (Daltons's Law). This may be prevented by an aircraft cabin or supplemented oxygen. Decompression sickness is very rare in aviation but divers should comply to a dive free interval before flying.

Aerospace Medicine↗

Daily diving pattern of Korean and Japanese breath-hold divers (ama).

Daily diving patterns and thoracic skin and sea-water temperatures were recorded during the entire work shift of Korean female unassisted (cachido) and Japanese male unassisted and partly assisted (funado) divers using Underwater Physiological Data Loggers developed in Buffalo and Japan. All 3 groups of divers were studied during the summer of 1989 and 1990. Additional studies were conducted during the winter of 1991 on Korean female divers who, unlike Japanese divers, dive all year round. The water temperature of the diving grounds in summer was 24 degrees C in both Korea and Japan, and 10 degrees C during winter in Korea. Both Korean female and Japanese male cachido divers made 113-138 dives a day and stayed in the water a total of 170-200 min.day-1, of which only 52-63 min were spent diving submerged, and the remaining time at the water surface. These diving patterns were not different between female and male cachido divers. Compared with Japanese male divers, Korean female divers dived to a shallower depth (3.7 vs. 6.9 m) with shorter dive time (29 vs. 37 s) and shorter bottom time (14 vs. 18 s). Velocities of descent (0.72 vs. 0.47 m.s-1) and ascent (0.77 vs. 0.56 m.s-1) were also slower in female divers than in male divers. The diving pattern of Korean female divers was similar in both summer and winter. Although all cachido divers wore wet suits and thus were protected from severe cold stress, thoracic skin temperature decreased during a work shift by 7 degrees C in winter (vs. 1 degree C in summer) in Korean divers. Compared with Japanese male cachido divers, Japanese male funado divers stayed in the diving ground (including time in the boat) longer (201 vs. 305 min.day-1) but performed only 23 dives per day. The average diving depth (9.7 m), duration (69 s), and bottom time for each dive (45 s), however, were significantly greater in funados. The velocity of vertical descent (1.0 m.s-1) was also significantly greater in funados because they descend with a weight (8-12 kg). The rate of ascent was not different.

Body Temperature↗

Distraction effect of cold water on performance of higher-order tasks.

Eight U.S. Navy-qualified scuba divers performed peripheral target detection and navigation problem-solving tasks continuously during 3-h exposures to moderate (15.5 degrees C) and cold (4.5 degrees C) water. Upon exiting the water, the divers did a series of arithmetic computations. Measures of physiological cold stress were periodically recorded, and estimates of changes in body heat content were calculated. Results suggest a significant distraction effect of cold water exposure on performance of high-order tasks. Hour-to-hour comparisons of task performance between the two exposures showed no significant differences except for the in-water tasks during the first hour of exposure. Furthermore, individual performance levels achieved during second and third hours of cold water exposure were significantly correlated with levels achieved in moderate water and not with individual differences in body cooling. It is recommended that the psychologically mediated effects of cold exposure be given greater attention in both research and operations.

Adult↗

Redistribution of air within the lungs may potentiate "fright" bradycardia in submerged crocodiles (Crocodylus porosus).

1. Voluntary undisturbed dives by Crocodylus porosus were short in duration (3.08 +/- 1.87 min, mean +/- SD) and accompanied by a small but significant bradycardia (14.3 +/- 5.9% drop). 2. When crocodiles were disturbed underwater there was a rapid onset of "fright" bradycardia, to 65 +/- 6.0% of surface heart rates and dive durations were prolonged to 19.6 +/- 1.8 min. 3. The development of "fright" bradycardia was not accompanied by any increase in intratracheal pressure or expulsion of lung gas. However, sustained contraction of the abdomen and expansion of the thorax revealed a redistribution of air anteriorly within the lungs. 4. We propose that the redistribution of air within the lungs may generate an afferent signal which potentiates the initiation of a severe, dive-prolonging bradycardia.

Afferent Pathways↗

Potent inhibition of endopeptidase 24.16 and endopeptidase 24.15 by the phosphonamide peptide N-(phenylethylphosphonyl)-Gly-L-Pro-L-aminohexanoic acid.

A phosphonamide peptide, N-(phenylethylphosphonyl)-Gly-L-Pro-L-aminohexanoic acid, previously shown to block Clostridium histolyticum collagenases, was examined as a putative inhibitor of endopeptidase 24.16 and endopeptidase 24.15. Hydrolysis of two endopeptidase 24.16 substrates, i.e. 3-carboxy-7-methoxycoumarin (Mcc)-Pro-Leu-Gly-Pro-D-Lys-dinitrophenyl (Dnp) and neurotensin, were completely and dose-dependently inhibited by the phosphonamide inhibitor with KI values of 0.3 and 0.9 nM respectively. In addition, the phosphonamide peptide inhibited the hydrolysis of benzoyl (Bz)-Gly-Ala-Ala-Phe-(pAB) p-aminobenzoate and neurotensin by endopeptidase 24.15 with about a 10-fold lower potency (KI values of 5 and 7.5 nM respectively). The selectivity of this inhibitor towards several exo- and endo-peptidases belonging to the zinc-containing metallopeptidase family established that a 1 microM concentration of this inhibitor was unable to affect leucine aminopeptidase, carboxypeptidase A, angiotensin-converting enzyme and endopeptidase 24.11. The present paper therefore reports on the first hydrophilic highly potent endopeptidase 24.16 inhibitor and describes the most potent inhibitory agent directed towards endopeptidase 24.15 developed to date. These tools should allow one to assess the contribution of endopeptidase 24.16 and endopeptidase 24.15 to the physiological inactivation of neurotensin as well as other neuropeptides.

Amino Acid Sequence↗

Heart rate and body temperature during free diving of Weddell seals.

We have developed and successfully used the first microprocessor-controlled monitors for collection of data on depth, heart rate, and body temperature of one fetal and five adult male freely swimming Weddell seals. Adult seals almost invariably experienced a prompt bradycardia at the start of each dive, and the mean heart rate during diving was significantly lower for dives greater than 20 min (P greater than 0.999). The heart rate was also significantly greater during the ascent portion of dives when compared with the descent portion (P greater than 0.95). The fetal seal experienced a slow onset of bradycardia when its mother dived; during diving the fetal heart rate decreased by an average of 1.1 beats/min for each minute of the dive. The fetal heart rate generally took approximately 10 min to recover to predive levels after its mother resurfaced to breathe. The body temperature of one adult male Weddell seal showed a decrease of greater than 1.5 degrees C from resting levels before dives of greater than 15 min were initiated and a drop of over 2 degrees C before dives of greater than 30 min duration.

Animals↗