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[The diagnosis of fat embolism in lungs altered by putrefaction].

The results of biochemical analysis of rabbit lungs in case of death due to fat embolism and mechanical asphyxia (control) are presented. Reliable difference in lipid quantities was evident both immediately after death and in different putrefaction periods (this difference was 9-10 times greater in case of pulmonary fat embolism than in controls). The significant reduction in water content of the lungs in case of fat embolism as compared to controls was detected. Histological analysis of the putrefactive lungs can't detect fat embolism. Biochemical analysis makes it possible to diagnose fat embolism of the lungs in case of their marked putrefactive changes.

Animals↗

Forensic taphonomy in fluvial water: A systematic review.

Human remains are frequently recovered from fluvial (lotic) water environments. Rivers, streams, canals and other flowing water bodies pose distinct challenges in forensic practice, particularly due to their current. Compared to other aquatic contexts, fluviatile taphonomy is underrepresented in the forensic literature and the last comprehensive summary was written over a decade ago. Therefore, a systematic review was performed to collect and summarise the current knowledge in forensics about fluvial taphonomy, including decomposition of remains, transport characteristics, search, postmortem submersion interval (PMSI) estimation, taphonomic alterations, and differences to other water bodies. 92 reports met the inclusion criteria for this review, most of them showed a moderate risk of bias. Their main findings were summarised and two major challenges identified. First, predictions about fluvial transport of human bodies pose a tremendous issue in forensic practice, leading to later detection and lower recovery rate of remains, complicating various subsequent analyses. Second, PMSI estimations are difficult particularly due to variable environmental conditions in fluviatile systems. Recommendations for future research in the area of fluvial forensic taphonomy include that case-related data such as the detailed decomposition state and river characteristics need to be made available. More standardised research protocols are required for a facilitated comparison between studies, environments and regions. This review provides forensic practitioners with a comprehensive, updated resource for the investigation of fluvial remains. It also guides future research towards a more efficient practice to build the basis for a broader, more robust and comprehensive synthesis, subsequently supporting analyses in forensic casework.

Humans↗

Changes of activity and isozyme pattern of phosphofructokinase in the brains of patients with Alzheimer's disease.

A severe reduction of the in vivo cerebral glucose consumption rate is generally found in patients with Alzheimer's disease. In postmortem studies changes in the activities of key regulatory glycolytic enzymes, including 6-phosphofructokinase (PFK), have been reported in Alzheimer's disease brains, but the results obtained so far are inconsistent and controversial. We reevaluated the activity of PFK in brain tissue from clinically and neuropathologically confirmed cases of Alzheimer's disease using optimized tissue disintegration and assay methods and determined the PFK isozyme pattern. PFK activity in brains from patients with Alzheimer's disease was significantly increased in frontal and temporal cortex and unchanged in the other brain areas studied when compared with control brains. All three PFK isozymes were detected in each of the brain areas studied. In brains of Alzheimer's disease patients the level of the C-type PFK was slightly reduced at the expense of the M- and L-type subunits. The data presented do not support the results of other groups, which reported up to a 90% reduction of PFK activity in Alzheimer's disease. In contrast, the data presented clearly rule out the suggestion that changes of PFK activity might be one of the causes for the reduced glucose consumption in Alzheimer's disease brains.

Aged↗

Delimitation of the time of death by immunohistochemical detection of calcitonin.

To improve the possibilities of delimitating the time of death after longer laytime it was examined if this is possible by immunohistochemical detection of calcitonin. The results show that in our examination material the c-cells of the thyroid glands of up to 4-day-old corpses produce a positive immunoreaction towards calcitonin in all cases whereas none of the corpses older than 13 days show such a reaction. This means that in the case of a negative immunoreaction the time of death can be assumed to lie >4 days before the autopsy. The fact that a negative immunoreaction occurred consistently after 13 days leads to the conclusion that when calcitonin has been stained in a specimen, the death of the respective person must lie a maximum of 12 days earlier, whereby these time-limits may change in considerably different surrounding conditions.

Calcitonin↗

Diffusion MRI in multiple sclerosis.

Diffusion imaging is a quantitative, MR-based technique potentially useful for the study of multiple sclerosis (MS), due to its increased pathologic specificity over conventional MRI and its ability to assess in vivo the presence of tissue damage occurring outside T2-visible lesions, i.e., in the so-called normal-appearing white and gray matter. The present review aims at critically summarizing the state-of-the-art and providing a background for the planning of future diffusion studies of MS. Several pieces of evidence suggest that diffusion-weighted and diffusion tensor MRI are sensitive to MS damage and able to detect its evolution over relatively short periods of time. Although a significant relationship between diffusion-weighted MRI findings and MS clinical disability was not found in the earliest studies, with improved diffusion imaging technology correlations between diffusion abnormalities and MS clinical aspects are now emerging. However, the best acquisition and postprocessing strategies for MS studies remain a matter of debate and the contribution of newer and more sophisticated techniques to diffusion tensor MRI investigations in MS needs to be further evaluated. Although changes in diffusion MRI indices reflect a net loss of structural organization, at present we can only speculate on their possible pathologic substrates in the MS brain. Postmortem studies correlating diffusion findings with histopathology of patients with MS are, therefore, also warranted.

Autopsy↗

Analysis of carbon monoxide.

The degree of exposure to carbon monoxide is most often assessed by measuring the blood carboxyhaemoglobin saturation. This measurement is relevant to investigations of acute accidental or deliberate poisoning and of chronic exposure in a domestic or work place environment. Simple spectrophotometric methods based on differential protein precipitation or dithionite reduction are prone to interference from other haemoglobin pigments and are imprecise for low-level estimations. Automated spectrophotometric devices (CO-oximeters) that estimate simultaneously total haemoglobin, percentage oxyhaemoglobin and percentage carboxyhaemoglobin have acceptable accuracy for carboxyhaemoglobin saturation levels of > 5% and are recommended for most clinical purposes. For the investigation of low-level exposure and the detection of increased haemolysis in neonates, more sensitive methods involving the release of carbon monoxide and its measurement by gas chromatography are required. Gas chromatographic methods are also appropriate when examining post-mortem blood samples where putrefaction or heat stress has resulted in a significant change in haemoglobin composition.

Breath Tests↗

Color matching and the Stiles-Crawford effect in observers with early age-related macular changes.

We studied the color-match-area effect and the Stiles-Crawford effect in 10 observers with age-related macular changes. Observers were graded on a scale of I to IV according to the Sarks classification, which correlates fundus appearance and visual acuity with the severity of postmortem histological changes in Bruch's membrane. Observers in group II showed subtle abnormalities of color matching; those in groups III and IV showed more-severe abnormalities. The Stiles-Crawford effect was abnormal in 9 of 10 eyes tested. Only one observer showed a Stiles-Crawford effect that had a well-defined peak near the center of the pupil and a near-normal bandpass. The results suggest that early age-related changes visible in the fundus can be revealed by psychophysical tests of photoreceptor architecture.

Aged↗

Delimitation of the time of death by immunohistochemical detection of thyroglobulin.

To improve the possibilities to delimit the time of death after longer laytime it was examined if this is possible by immunohistochemical detection of thyroglobulin. The results show that in our examination material the colloid and the follicular cells of the thyroid glands of up to 5-day-old corpses produce a positive immunoreaction towards thyroglobulin in all cases whereas none of the corpses older than 13 days show such a reaction. This means that in case of a negative immunoreaction the time of death can be assumed to lie more than 6 days before the autopsy. The fact that a negative immunoreaction occurs consistently after 13 days leads to the conclusion that when thyroglobulin has been stained in a specimen, the death of the respective person must lie a maximum of 12 days earlier, whereby these time-limits may change in considerably different surrounding conditions.

Death↗