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Results for “Retinal Horizontal Cells”

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PubMed1993

In vitro phosphorylation in isolated horizontal cells of the fish retina: effects of the state of light adaptation.

Horizontal cells, which are second-order neurons of the vertebrate retina, exhibit synaptic plasticity governed by light and dark adaptation. We have investigated the alterations in the protein phosphorylation patterns of isolated carp (Cyprinus carpio) horizontal cells in relation to their state of light adaptation by using an in vitro phosphorylation assay and compared the resulting data with protein synthesis patterns of the whole retina. Phosphoproteins and [35S]methionine-labelled proteins were analysed by one- and two-dimensional gel electrophoresis followed by autoradiography. The state of light adaptation significantly affected the in vitro phosphorylation of horizontal cell proteins with molecular weights of 68, 56/58, 47, 28 and 15 kDa, but had no effect on the protein synthesis of retinal proteins. In the light the most prominent increase of 32P incorporation was observed in the 47 kDa protein. The biochemical properties of this protein closely resembled those of the growth-associated GAP-48, found in the fish retina. In addition, the phosphorylation of horizontal cell homogenates in the presence of protein kinase activators such as cyclic AMP, calcium, calmodulin and phospholipids revealed that horizontal cells of the fish retina contain cyclic AMP-, calcium/calmodulin- and calcium/phospholipid-dependent protein kinase activity resulting in the phosphorylation of several horizontal cell proteins, including the phosphoproteins which were affected by the state of light adaptation.

Adaptation, Physiological↗

PubMed1979

Demonstration of a somatostatin-like activity in retinal cells of the rat.

A somatostatin-like substance is demonstrated by light microscopic immunohistochemistry (PAP-method) in perikarya and cell processes of the retina of adult and infant rats. These perikarya are identified according to their size, arrangement and distribution. Each of the first two neuronal orders (receptors, bipolar cells, ganglionic cells) of the visual pathway can be associated with retinal cells reacting positively with anti-somatostatin. In the adult rat, perikarya and processes of (i) horizontal cells, (ii) amacrine cells and (iii) large neurons in the ganglionic layer are specifically labeled. The staining of middle-sized and small ganglion cells is probably caused by the close attachment of labeled fibers to non-reacting cells. Postnatally, the immunoreactive elements develop in parallel to the differentiation of the corresponding retinal layers. It is discussed whether the three types of retinal cells containing a somatostatin-like substance provide an inhibitory system to each of the two orders of retinal neurons.

Age Factors↗

PubMed1997

Light exposure induces ubiquitin conjugation and degradation activities in the rat retina.

PURPOSE: To evaluate the consequences of light exposure on retinal ubiquitin (Ub) conjugation and degradation. METHODS: Two-month-old Long Evans pigmented rats were exposed to constant light (180 foot-candles) or were left in complete darkness for 18 hours. Rats used for cyclic light and diurnal rhythm experiments were removed from their light cycles at different times (24-hour clock): 0700 (before the light was turned on), 1000 (3 hours into the light cycle), 1000D (continued in the dark cycle), 1900 (before the light was turned off), 2200 (3 hours into the dark cycle), and 2200L (continued in the light cycle). The retinas were examined for Ub conjugation, adenosine triphosphate-Ub-dependent degradation, levels of Ub messenger RNA, and localization of Ub immunocytochemistry. RESULTS: There was a statistically significant increase in Ub conjugation and degradation in retinas isolated from light-exposed animals compared with degradation in retinas of dark-adapted animals. However, no significant differences were observed in the levels of Ub messenger RNA from cyclic light, or light-exposed or dark-adapted retinas, suggesting that light-stress-induced changes do not reflect increased transcriptional activity. The daily variations observed in Ub conjugation and degradation suggest that these processes are probably the result of a circadian rhythm. Results of immunohistochemical studies revealed that Ub and its conjugates were uniformly distributed throughout the retinal cell layers in light- and dark-adapted retinas. However, in light-exposed retinas, a strong positive immunoreactivity was observed in the inner retina, specifically in horizontal and ganglion cells. CONCLUSIONS: These results suggest that light exposure may play a role in inducing Ub-conjugating activity in certain retinal cells. Furthermore, the results support the hypothesis that Ub is a stress protein that plays an important role in protecting cells under stress conditions.

Adenosine Triphosphate↗

PubMedDate not supplied

Development of the rabbit retina, III: Differential retinal growth, and density of projection neurons and interneurons.

To provide a quantitative description of postnatal retinal expansion in rabbits, a new procedure was developed to map the retinae, which cover the inner surface of hemispheres or parts of rotation ellipsoids, in situ, onto a single plane. This method, as well as the known distribution of Müller cells per unit retinal surface area, were used to estimate the redistribution of specific subpopulations of Müller cells within different topographic regions of the retinae. Müller cells are known to exist as a stable population of cells 1 week after birth and can therefore be used as "markers" for determining tissue expansion. Our results show that differential retinal expansion occurs during development. Peripheral retinal regions expand at least twice as much as the central ones. Furthermore, there is a greater vertical than horizontal expansion. This differential retinal expansion leads to a corresponding redistribution of 5-hydroxytryptamine (5-HT) accumulating amacrine cells. Differential retinal expansion, however, does not account for all of the changes in the centro-peripheral density gradient of cells in the ganglion cell layer (GCL)--mostly retinal ganglion cells--during postnatal development. The changes in the ganglion cell layer were evaluated in Nissl-stained wholemount retinal preparations. Additionally, the difference between expansion-related redistribution of cells in the GCL and Müller cells was confirmed in wholemount preparations where Müller cells (identified as vimentin positive) and cells in the GCL (identified by fluorescent supravital dyes) were simultaneously labeled. It is assumed that many of the ganglion cells within the retinal center are not translocated during retinal expansion, possibly because their axons are fixed. In contrast, 5-HT accumulating amacrine cells--which are interneurons without a retinofugal axon--display a passive redistribution together with the surrounding retinal tissue.

Animals↗

PubMed2003

Mosaic regularity of horizontal cells in the mouse retina is independent of cone photoreceptor innervation.

PURPOSE: To determine whether the density and the mosaic regularity of the population of horizontal cells is dependent on innervation from the cone photoreceptors by comparing these features in wild-type and transgenic mice expressing an attenuated diphtheria toxin in the cones. METHODS: Retinal wholemounts from coneless transgenic mice and their wild-type littermates were immunostained for calbindin or for cone opsins, and labeled cells and outer segments were counted to determine horizontal cell and photoreceptor density. The x-y positional coordinates of each horizontal cell were also determined, from which the geometrical properties of the horizontal cell mosaic were examined using nearest-neighbor and Voronoi domain analyses. Autocorrelation and density-recovery profile analyses were also conducted to identify the presence of exclusion zones within the population of horizontal cells. For each sampled field, random simulations of matched density, constrained by the physical size of the horizontal cells, were generated in parallel and analyzed with the real data. RESULTS: Coneless mice were confirmed to contain only 3% of the normal cone photoreceptor population. Despite the loss of these afferents, horizontal cell density did not differ between the wild-type and coneless retinas. Mosaic regularity in wild-type and coneless retinas did not differ, but each differed significantly from random simulations of identical density. Horizontal cells in both the wild-type and coneless mouse retina exhibited exclusion zones extending beyond the physical size of the soma, suggested to reflect intercellular interactions during early development that drive tangential dispersion; these were slightly larger in the wild-type retina. CONCLUSIONS: Cone innervation is not a necessary condition for horizontal cell survival during postnatal development. The resiliency of the regularity in the horizontal cell mosaic is consistent with the hypothesis that such global patterning is an emergent property of these cells as they engage in local interactions that are largely independent of their afferent innervation.

Animals↗

PubMed1991

Substance P-immunoreactive neurons in the retina of two lizards.

The dendritic morphology and retinal distribution of substance P(SP)-immunoreactive neurons was determined in two Australian lizard species Pogona vitticeps and Varanus gouldii, by using immunohistochemistry on retinal wholemounts and sectioned materials. In both species, two classes of SP-immunoreactive neurons were described in the inner nuclear layer (INL) and classified as amacrine cells (types A and B). Type A amacrine cells had large somata and wide-field, bistratified dendrites branching in sublaminas 1 and 5 of the inner plexiform layer (IPL). Their morphology and retinal distribution differed between the two species. Type B amacrine cells in both species had small somata and small-field dendritic branching. A population of SP-immunoreactive neurons with classical ganglion cell morphology were identified in the ganglion cell layer (GCL). Immunostained ganglion cells occurred in larger numbers of Varanus gouldii than in Pogona vitticeps. In both species type B SP cells were the most numerous and were estimated to be about 60,000-70,000. They were distributed non-uniformly with a high density band across the horizontal meridian of the retina, from where the density decreased towards the dorsal and ventral retinal margins. In both species type A amacrine cells occurred in small numbers distributed sparsely in the peripheral retina. The faint immunostaining of SP-immunoreactive neurons in the GCL, did not allow us to reliably determine their numbers and retinal distribution. The functional significance of SP-immunoreactive amacrine and ganglion cells in the lizard retina remains to be determined.

Animals↗

PubMed2006

Expression of pannexin family of proteins in the retina.

Expression of the Panx1 and Panx2 members of the pannexin family of gap junction proteins was studied in the retina by in situ hybridization and qRT-PCR. Both pannexins showed robust expression across the retina with predominant accumulation in the retinal ganglion cells (RGCs). In concordance, immunohistochemical analysis showed accumulation of the Panx1 protein in RGCs, amacrine, horizontal cells and their processes. Two Panx1 isoforms were detected: a ubiquitously expressed 58 kDa protein, and a 43 kDa isoform that specifically accumulated in the retina and brain. Our results indicated that Panx1 and Panx2 are abundantly expressed in the retina, and may therefore contribute to the electrical and metabolic coupling, or to signaling between retinal neurons via the secondary messengers.

Amacrine Cells↗

PubMed1983

Responses of units in the rat cerebellar flocculus during optokinetic and vestibular stimulation.

The simple (SS) and complex spike (CS) responses of Purkinje (P-cells) and non-Purkinje (non P-cells) in the cerebellar flocculus were studied in alert pigmented rats (DA-HAN) during binocular and monocular optokinetic stimulation (OKS), vestibular stimulation and a combination of the two. Of a total of 98 P-cells whose SS discharges were activated by rotary stimulation of the horizontal canal in the dark (type I and type II P-cells), the vast majority (72%) responded to constant velocity binocular OKS that was produced by means of a horizontal shadow projector system. The remaining P-cells responded only to vestibular stimulation (19%), to OKS or to the presumed fast components of optokinetic and vestibular nystagmus (9%). The optokinetic responses of P-cells were generally bidirectional but asymmetrical, i.e., the increases in rate in one direction were larger in magnitude than decreases on opposite OKS and were synergistic with the semicircular canal input. During constant velocity OKS, the discharge of a few P-cells rose approximately exponentially, outlasted the stimulus by as much as 10-13.5s and, thus, resembled OKS responses of vestibular nucleus neurons. However, the majority exhibited a phasic-tonic response governed by a short "time constant" of from 0.5-3s. The velocity tuning curves of vestibular/OKS responding P-cells showed peak sensitivities with retinal slip velocities of 1.5-2 degrees/s. This is higher than the ca. 1 degree/s determined for other relay nuclei of the horizontal optokinetic pathway. The responses of non P-cells suggest that they originate from mossy fiber projections from vestibular, visual (optokinetic) and saccadic eye movement-related areas of the brainstem. Most of the units carried a combined vestibular and optokinetic signal. The majority showed a bidirection-selective response to OKS, and a small percentage showed unidirectional responses only. Monocular testing of P-cells revealed that most received a bidirection-selective, but asymmetrical, OKS input. Slightly more than half of these had a strongest OKS drive from the contralateral eye; the remaining units were driven most strongly by the ipsilateral eye. Unidirection-selective P-cells, driven by OKS to the ipsi- or contralateral eye, were uncommon; yet this class is common among other portions of the horizontal optokinetic system (e.g., vestibular nuclei, praepositus hypoglossi nucleus, nucleus reticularis tegmenti pontis).(ABSTRACT TRUNCATED AT 400 WORDS)

Acoustic Stimulation↗

PubMed2002

The cyclin-dependent kinase inhibitors p19(Ink4d) and p27(Kip1) are coexpressed in select retinal cells and act cooperatively to control cell cycle exit.

Cyclin-dependent kinase inhibitors (cdki's), including p19(Ink4d) and p27(Kip1), mediate exit from the cell cycle. To determine the function of these cdki's in regulating neurogenesis, we examined retina from wild-type, Ink4d-null, and Ink4d/Kip1-double null animals. Ink4d was expressed in progenitors and select neurons in the mature retina. Ink4d-null retina showed an extended period of proliferation, followed by apoptosis. Colabeling for p19(Ink4d) and p27(Kip1) revealed that a subpopulation of cells expressed both inhibitors. Deletion of Ink4d and Kip1 resulted in continued proliferation that was synergistic. This hyperproliferation led to an increase in number of horizontal cells and differentiated neurons reentering the cell cycle. Deletion of Ink4d and Kip1 also exacerbated the retinal dysplasia observed in Kip1-null mice, which was shown to be partly dependent on p53. These data indicate that select retinal cells express both p19(Ink4d) and p27(Kip1) and that they act cooperatively to ensure cell cycle exit.

Amacrine Cells↗

PubMed1991

Relation of an array of early-differentiating cones to the photoreceptor mosaic in the primate retina.

The retina of diurnal primates, including humans, contains a reiterative mosaic of red-, green- and blue-sensitive cones whose visual pigments are maximally sensitive to long, middle or short wavelengths, respectively. Although the distribution of the cone subtypes in the adult rhesus monkey has been quantified using opsin-specific antisera, the mechanism for the phenotypic specification of the cone subtypes and the establishment of their ratios in the retinal mosaic remain unknown. Here we present immunocytochemical evidence that a subset of cones (about 10%) express their cell-specific opsin two to three weeks before the surrounding cones. Remarkably, these precocious cones are evenly stationed throughout undifferentiated regions of the retinal surface from several weeks after their last mitotic division, and at least one month before the formation of their synapses with bipolar and horizontal cells. Use of confocal laser microscopy reveals that the inner segments of immunolabelled and surrounding unlabelled cones are transiently in apposition with one another, enabling surface mediated interactions to occur during this period. We suggest that the early maturing cones induce neighbouring undifferentiated cones to express an appropriate opsin phenotype, and therefore constitute a 'protomap' for the emergence of the species-specific retinal mosaic.

Animals↗

PubMed2006

Silencing acid-sensing ion channel 1a alters cone-mediated retinal function.

The action of extracellular protons on retinal activity and phototransduction occurs through pH-sensitive elements, mainly membrane conductances present on the different cell types of the outer and inner nuclear layers and of the ganglion cell layer. Acid-sensing ion channels (ASICs) are depolarizing conductances that are directly activated by protons. We investigated the participation of ASIC1a, a particular isoform of ASICs, in retinal physiology in vivo using electroretinogram measurements. In situ hybridization and immunohistochemistry localized ASIC1a in the outer and inner nuclear layers (cone photoreceptors, horizontal cells, some amacrine and bipolar cells) and in the ganglion cell layer. Both the in vivo knockdown of ASIC1a by antisense oligonucleotides and the in vivo blocking of its activity by PcTx1, a specific venom peptide, were able to decrease significantly and reversibly the photopic a- and b-waves and oscillatory potentials. Our study indicates that ASIC1a is an important channel in normal retinal activity. Being present in the inner segments of cones and inner nuclear layer cells, and mainly at synaptic cleft levels, it could participate in gain adaptation to ambient light of the cone pathway, facilitating cone hyperpolarization in brightness and modulating synaptic transmission of the light-induced visual signal.

Acid Sensing Ion Channels↗

PubMed1981

Time-dependent decrease in the extent of visual deafferentation in the lateral geniculate nucleus of adult cats with small retinal lesions.

Small, round photocoagulator lesions of 3-6 degrees (0.6-1.2 mm) diameter were placed nasally on the retina of adult cats. Histological controls proved the complete destruction of all retinal layers within the lesions. Changes in lesion size by shrinkage of the retinal scar did not exceed 0.1 mm or 0.5 degrees. At different times after photocoagulation, single cells were recorded in layers A and A1 of the contralateral lateral geniculate nucleus (LGN) with tungsten microelectrodes. Acute lesions of this size completely deafferented single LGN cells in layer A whose receptive field (RF) area was within the lesion. Thirty days and more after coagulation, light-excitable cells were detected in the originally deafferented LGN region, with RFs in the immediate surround of the retinal lesion. The spontaneous activity and light excitability of these neurons were altered. The representation of 3-4 degrees retinal lesions at 20 degrees horizontal eccentricity was found to be completely filled in by excitation in the LGN. Excitation had spread from the unsevered parts of the retina into the region of deafferentation. Single cells with signs of multiple activation from more than one border region of the retinal lesion were occasionally detected.

Animals↗

PubMed2001

Developmental expression of the small-conductance Ca(2+)-activated potassium channel SK2 in the rat retina.

Small-conductance Ca(2+)-activated potassium (SK) channels are present in most central neurons, where they mediate the afterhyperpolarizations (AHPs) following action potentials. SK channels integrate changes in intracellular Ca(2+) concentration with membrane potential and thus play an important role in controlling firing pattern and excitability. Here, we characterize the expression pattern of the apamin-sensitive SK subunits, SK2 and SK3, in the developing and adult rat retina using in situ hybridization and immunohistochemistry. The SK2 subunit showed a distinct and developmentally regulated pattern of expression. It appeared during the first postnatal week and located to retinal ganglion cells and to subpopulations of neurons in the inner nuclear layer. These neurons were identified as horizontal cells and dopaminergic amacrine cells by specific markers. In contrast to SK2, the SK3 subunit was detected neither in the developing nor in the adult retina. These results show cell-specific expression of the SK2 subunit in the retina and suggest that this channel underlies the apamin-sensitive AHP currents described in retinal ganglion cells.

Amino Acid Sequence↗

PubMed1987

Suppressive rod-cone interaction in distal vertebrate retina: intracellular records from Xenopus and Necturus.

The influence of dim diffuse adapting fields upon the sensitivity to focal photic stimulation was studied by means of intracellular recording in retinal neurons of the south african clawed frog, Xenopus and the mudpuppy, Necturus. In cones and in most horizontal and bipolar cells lacking color opponency, dim diffuse backgrounds have little influence upon the response to diffuse flicker of low (less than 2 Hz) temporal frequencies; however, with small diameter test probes of higher temporal frequencies, presentation of dim backgrounds enhance the peak-to-peak amplitude of responses to sinusoidal flicker by as much as 800%. This background enhancement effect adheres to the spectral sensitivity of the green-absorbing rod photopigment, and appears to be largely independent of the influence of the adapting field upon cone photopigment or ambient membrane potential in the recorded neuron. This effect cannot be obtained with rod-driven flicker responses. We designate this background influence on flicker, suppressive rod-cone interaction (SRCI) and attribute it to a tonic suppressive (probably inhibitory) influence of rods upon cone pathways that is removed by rod light adaptation. SRCI is also observed in the response of most sustained ON and OFF ganglion cells. However, no corresponding effect occurs in rods, color-opponent second-order neurons, ON-OFF amacrine cells, or most ON-OFF ganglion cells. The spatial and temporal limitations of SRCI observed by means of intracellular recording in amphibians are very similar to those documented by means of psychophysical or electroretinogram (ERG) procedures in a wide variety of species including humans (2, 4, 11, 22, 23, 29). SRCI most probably reflects a process that is mediated by horizontal cells. The specifics of the underlying mechanism remain unclear.

Adaptation, Ocular↗

PubMed1998

Protection against glutamate neurotoxicity in retinal cultures by acidic conditions.

We evaluated the effects of extracellular acidic conditions on glutamate-induced death in cultured retinal neurons. Primary retinal cultures, obtained from 3- to 5-day-old Wistar rats, were estimated to be consisted of mainly amacrine cells (90%) together with a small population of horizontal (8%) and ganglion cells (2%). We examined the effects of acidic pH (pH 6.0 to 7.0) on glutamate neurotoxicity by monitoring the delayed death of retinal neurons induced by brief (10 min) exposure to 1 mM glutamate followed by a 24-hr incubation. The glutamate-induced delayed death of cultured retinal neurons was attenuated with an acidic pH between 6.0 and 7.0. Furthermore, whole-cell patch-clamp recordings were taken from retinal neurons to examine the effects of acidic pH on N-methyl-D-aspartate (NMDA) or kainate receptor-mediated currents. NMDA- and kainate-induced currents were suppressed at pH 6.0 to 7.0 and pH 6.0 to 6.5, respectively. The acidity of the medium protected the retinal neurons from glutamate-induced delayed death, probably by inhibiting NMDA and/or kainate receptor activation.

6-Cyano-7-nitroquinoxaline-2,3-dione↗

PubMed1977

The response of cat horizontal cells to flicker stimuli of different area, intensity and frequency.

In the cat's retina we analyzed the sinewave light flicker response nonlinearity of Hm-type horizontal cells. (For a description of the three types of dynamic responses of cat retinal H-units see Foerster et al., 1977). For equal decreases in the Hm-response amplitude an increase in stimulus frequency had a much stronger linearizing effect than a decrease in stimulus area. Thus the distortion is not simply proportional to response amplitude. Both Hm- and Hn-units had frequency dependent nonlinear area-response functions. The receptive field of Hm-units increased dramatically with stimulus frequency, e.g. from 1 degrees at 1 Hz to 8 degrees or more at 44 Hz. Intensity transfer data could be described by the function Lb-(sigmab + Lb)-1 with b approximately 1 for Hm-units and b approximately 0.5 for Hn-units. Distortion values predicted from intensity transfer data were too high. It was also found that higher harmonics were attenuated more than the first harmonic at increasing frequencies. Therefore it appeared that some filtering preceded the amplitude compression stage.

Animals↗

PubMed1999

Centrifugal Phe-Met-Arg-Phe-NH2-like immunoreactive innervation of the retina in a non-teleost bony fish, Lepisosteus osseus.

In all teleosts studied, Phe-Met-Arg-Phe-NH2- (FMRFamide-) like immunoreactive fibers originating from structures related to the olfactory system project to the retina. A complete report on this olfacto-retinalis projection in fish that are phylogenetically older than teleosts is still missing. We have visualized FMRFamide-like immunoreactive fibers in the optic nerve, the optic chiasm and in the retina of the longnose gar, Lepisosteus osseus. They terminate on amacrine or horizontal cells in the internal nuclear layer. Additionally, some of them appear to contact retinal ganglion cells.

Animals↗

PubMed1992

Abnormal electroretinography in schizophrenic patients with a history of sun gazing.

Electroretinographic (ERG) measurements were performed in 9 schizophrenic patients and in 13 control subjects. The measurements of schizophrenic patients as a group did not differ from those of normals. However, 6 schizophrenic patients who had a past history of sun gazing showed a decrease in retinal responsiveness under conditions of light adaptation. These results suggest that a subgroup of schizophrenic patients, who show deviant light-related behavior, have abnormal ERG. We postulate that an abnormality in retinal dopaminergic neurons, which are known to reduce light responsiveness of horizontal and ganglion cells, is the underlying pathophysiology of this clinical finding.

Adaptation, Ocular↗