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PubMed1990

[Experimental study on a mechanical root canal enlargement].

It is difficult for us to enlarge a root canal by using hand-instruments in a molar tooth, so we experimented by using mechanical equipment. We examined the human extracted premolar which we had preserved in physiological saline solution by using a speculative root canal enlarging machine. Then we observed the time and condition of enlarging root canal by X-ray. After slicing off, we examined with our eyes and with an electronic microscope. Afterward, we compared hand-instruments with the enlarging machine. As the results of this experiment; 1. Time by hand filing instruments took three or four times to make a root canal enlargement by a speculative enlarging machine. 2. After enlarging a root canal, we made a root canal filling. Observing them by X-ray and ground specimen, we recognized a good result in the root canal enlargement and in the condition of the root canal filling. 3. Observing the figure by electronic microscope, in the case of using a speculative machine, we found that the slicing aspects of dentin which had been enlarged by root canal were thoroughly smoothed in the center part of the root canal, but it was not smoothed in the root apex region. 4. It had a tendency to remain part of the cutting dentin near the apical area both by using hand cutting instruments and by using a speculative machine.

Bicuspid↗
PubMed2001

Aluminium tolerance in plants and the complexing role of organic acids.

The aluminium cation Al(3+) is toxic to many plants at micromolar concentrations. A range of plant species has evolved mechanisms that enable them to grow on acid soils where toxic concentrations of Al(3+) can limit plant growth. Organic acids play a central role in these aluminium tolerance mechanisms. Some plants detoxify aluminium in the rhizosphere by releasing organic acids that chelate aluminium. In at least two species, wheat and maize, the transport of organic acid anions out of the root cells is mediated by aluminium-activated anion channels in the plasma membrane. Other plants, including species that accumulate aluminium in their leaves, detoxify aluminium internally by forming complexes with organic acids.

Acetyl Coenzyme A↗
PubMed2002

Effects of nitrate pulses on BnNRT1 and BnNRT2 genes: mRNA levels and nitrate influx rates in relation to the duration of N deprivation in Brassica napus L.

A de-repression mechanism based on the disappearance of 'signals' down-regulating N transporter activity has been proposed in the literature to explain the transient increase of NO(3)(-) uptake by the roots following N deprivation in higher plants. This hypothesis was investigated at the physiological and molecular levels by measuring NO(3)(-) influx into roots of Brassica napus L. grown under low or high external concentrations of KNO(3) following N deprivation. Parallel measurements were made of endogenous NO(3)(-), amino acid concentrations and abundance of mRNA for BnNRT1 and BnNRT2, genes encoding nitrate-inducible transport proteins. The effect of NO(3)(-) pulsing on NO(3)(-) transport components in N-deprived plants was also investigated by measuring influx of high- and low-affinity transport system (HATS and LATS) and assaying mRNA levels. Influx of NO(3)(-) via HATS and LATS, and transcript levels of BnNRT2 and BnNRT1 decreased with the duration of N deprivation. The results suggested that the absence of de-repression of NO(3)(-) influx and BnNRT2 gene expression following N starvation was related to a high amino acid status. Pulsing with NO(3)(-) induced a large increase in BnNRT2 mRNA level, but a comparatively small increase in NO(3)(-) influx via HATS. The level of BnNRT1 mRNA also increased, but there was no effect on LATS uptake activity. The absence of a strict correlation between the NO(3)(-) transport activity and the mRNA BnNRT1 and BnNRT2 levels is discussed in terms of possible post-transcriptional regulation by the amino acids.

Amino Acids↗
PubMed2004

A novel symbiotic nitrogen-fixing member of the Ochrobactrum clade isolated from root nodules of Acacia mangium.

Ten strains of root nodule bacteria were isolated from the nodules of Acacia mangium grown in the Philippines and Thailand. Partial sequences (approx. 300 bp) of the 16S rRNA gene of each isolate were analyzed. The nucleotide sequences of strain DASA 35030 indicated high homology (>99%) with members of the genus Ochrobactrum in Brucellaceae, although the sequences of other isolates were homologous to those of two distinct genera Bradyrhizobium and Rhizobium. The strain DASA 35030 was strongly suggested to be a strain of Ochrobactrum by full length sequences of the 16S rRNA gene, fatty acids composition, G+C contents of the DNA, and other physiological characteristics. Strain DASA 35030 induced root nodules on A. mangium, A. albida and Paraserianthes falcataria. The nodules formed by strain DASA 35030 fixed nitrogen and the morphology of the nodules is the same as those of nodules formed by the other isolates. This is the first report that the strain of Ochrobactrum possesses complete symbiotic ability with Acacia.

Acacia↗
PubMed2007

Epidermal pattern formation in the root and shoot of Arabidopsis.

Root hair formation, stomata development on hypocotyls and trichome formation on leaves in Arabidopsis represent three model systems for epidermal patterning in plants that involve a common set of genes or corresponding homologues. The resulting pattern and the developmental readout are, however, strikingly different. Trichomes become regularly spaced on the leaf surface. Root hairs and stomata-bearing cells are formed in rows at specific locations with reference to the underlying cortex cells. In this review, we summarize the mechanistic similarities and discuss differences that might account for the different outcome of patterning in each system.

Arabidopsis↗
PubMed1996

The role of lipochitooligosaccharides in root nodule organogenesis and plant cell growth.

Lipochitooligosaccharides (Nod signals) excreted by rhizobia induce the formation of symbiotic root nodules in leguminous plants. This process is host plant specific, depending on the structural modifications of Nod signals. Rapid responses of plant roots in single cell assays have provided powerful tools in dissecting Nod signal transduction pathways and in elucidating the molecular basis of host specificity. Recent findings indicate that lipochitooligosaccharides, as well as symbiosis-related genes, also function in non legumes, pointing to a general role for these elements in plant morphogenesis.

Antigens, Bacterial↗
PubMed2006

Influence of cytokinins, auxins and polyamines on in vitro mass multiplication of cotton (Gossypium hirsutum L. cv. SVPR2).

In the present investigation, the influence of different forms of cytokinins, auxins and polyamines were tested for mass multiplication and regeneration of cotton. Initially, for the identification of effective concentration for multiple shoot induction, various concentrations of BAP, Kin and 2iP along with IAA and NAA were tested. Among tested concentrations, media fortified with MS salts; B5 vitamins; 30 g/l, glucose; 2.0 mg/l, 2iP; 2.0 mg/l, IAA and 0.7 % agar showed best response for multiplication of shoot tip explants (20 shoots per shoot tip explants). In nodal explants, maximum of 18.6 shoots were obtained in the media fortified with MS salts, B5 vitamins, 30 g/l, glucose, 2.0 mg/l, 2iP, 1.0 mg/l, NAA and 0.7 % agar. Effect of different concentrations of polyamines like spermidine and putrescine were also tested along with the above said multiplication media. Among the various treatments, 20 mg/l of putrescine showed best response and the multiple of shoots were increased to 26.5 shoots per shoot tip explants and 24.5 shoots per nodal explants. Elongation of shoots was achieved on multiple shoot induction medium. Significant number of roots were initiated in the medium supplemented with MS salts, vitamin B5 and IBA (2.0 mg/l). The frequency of root induction was increased by addition of, PVP (10 mg/l) along with root induction medium and after 2 weeks, the roots reached the maximum length of 22 cm. Further, these plantlets were hardened by using sand, soil and vermiculate in 1:1:1 ratio. The hardened plants were transferred to the environmental growth chamber for proper acclimatization. The hardened plants were then transferred to field for boll yielding and they exhibited 100% survival.

Aluminum Silicates↗
PubMed2005

In vitro multiple shoot regeneration and plant production in Alysicarpus rugosus DC. var. heyneanus Baker.

A protocol for in vitro multiple shoot regeneration and plant production through seedling (shoot tip) culture was established for Alysicarpus rugosus DC. var. heyneanus Baker. Maximum number of adventitious shoots (14.4) per shoot tip explant were initiated after two subcultures on MS solid medium supplemented with IAA (2.85 microM) plus BAP (2.22 microM) after 4 weeks. Shoot elongation (3.0-3.5 cm) was achieved on MS medium without any hormones. Stunted shoots elongated on half MS medium without growth hormones. Rooting occurred in MS medium containing IAA (1.14 - 2.85 microM) alone or in combination with IBA (0.89 - 2.46 microM) and or NAA (1.07 - 2.69 microM). Maximum rooting was established in MS medium supplemented with IAA (2.85 microM). The plants were acclimatized successfully with 55% survival in pot containing cocoa peat and sand (1:1). After a month, hardened plants were transferred to pots with manure, garden soil and sand (1:2:1) for further growth and finally planted in field.

Culture Techniques↗
PubMed1989

Differential growth and plant tropisms: a study assisted by computer simulation.

Tropisms and other movements of a plant organ result from alterations in local rates of cell elongation and a consequent development of a growth differential between its opposite sides. Relative elemental rates of elongation (RELELs) are useful to characterize the pattern of growth along and round an organ. We assume that the value of the RELEL at a given point is dependent on distance from the tip and that the distribution of values along the organ surface can be characterized in terms of the spread and the position of the maximum value. A computer model is described which accommodates these parameters and simulates tropic curvatures due to differential growth. Additional regulatory functions help to return the simulated organ to its original orientation. Particular attention is given to the simulation of root gravitropism because here not only do each of the various growth and regulatory parameters have a known biological counterpart, but some can also be given an actual quantitative value. The growth characteristics relate to the biophysical properties of cells in the elongation zone of the root, while the regulatory functions relate to aspects of the graviperception and transmission systems. We believe that, given a suitably flexible model, computer simulation is a powerful means of characterizing, in a quantitative way, the contribution of each parameter to the elongation of plant organs in general and their tropisms in particular.

Biophysical Phenomena↗
PubMed2006

Elevated CO2 stimulates net accumulations of carbon and nitrogen in land ecosystems: a meta-analysis.

The capability of terrestrial ecosystems to sequester carbon (C) plays a critical role in regulating future climatic change yet depends on nitrogen (N) availability. To predict long-term ecosystem C storage, it is essential to examine whether soil N becomes progressively limiting as C and N are sequestered in long-lived plant biomass and soil organic matter. A critical parameter to indicate the long-term progressive N limitation (PNL) is net change in ecosystem N content in association with C accumulation in plant and soil pools under elevated CO2. We compiled data from 104 published papers that study C and N dynamics at ambient and elevated CO2. The compiled database contains C contents, N contents, and C:N ratio in various plant and soil pools, and root:shoot ratio. Averaged C and N pool sizes in plant and soil all significantly increase at elevated CO2 in comparison to those at ambient CO2, ranging from a 5% increase in shoot N content to a 32% increase in root C content. The C and N contents in litter pools are consistently higher in elevated than ambient CO2 among all the surveyed studies whereas C and N contents in the other pools increase in some studies and decrease in other studies. The high variability in CO2-induced changes in C and N pool sizes results from diverse responses of various C and N processes to elevated CO2. Averaged C:N ratios are higher by 3% in litter and soil pools and 11% in root and shoot pools at elevated relative to ambient CO2. Elevated CO2 slightly increases root:shoot ratio. The net N accumulation in plant and soil pools at least helps prevent complete down-regulation of, and likely supports, long-term CO2 stimulation of C sequestration. The concomitant C and N accumulations in response to rising atmospheric CO2 may reflect intrinsic nature of ecosystem development as revealed before by studies of succession over hundreds to millions of years.

Carbon↗
PubMed2001

Host-plant recognition by parasitic Scrophulariaceae.

Parasitic plants in the Scrophulariaceae invade the roots of neighboring plants in order to rob them of water and nutrients. A distinctive feature of these parasites is their ability to cue their development to small molecules released by host-plant roots. Evidence is continuing to emerge that parasite perception of host factors occurs via a redox-associated mechanism. Genes predicted to function during the early stages of parasite-host interactions have been cloned from both plant partners, and their characterization is providing a genetic framework on which to model subterranean plant-plant interactions.

Benzoquinones↗
PubMed2001

Recovery of gravitropic response during regeneration of root caps does not require developed columella cells and sedimentation of amyloplasts.

Correlations between regeneration of the root cap and recovery of a gravitropic response were studied using primary roots of Phaseolus vulgaris. After removal of various lengths of the root tip a gravistimulus was continuously given to the root. The statistical analysis of data showed that recovery of the gravitropic response was gradually delayed as the length of the tips removed increased. This suggested that the columella cells of the root cap were involved in gravitropism. When the root cap was completely removed, the roots did not respond to gravistimuli for the first 15 h and began to reorient their growth direction at 20 h. At this time, the columella cells had just begun to regenerate and had immature amyloplasts which did not sufficiently form a sediment. These results suggest that other systems of perception exist in plant cells in addition to the amyloplast-based model of graviperception.

Fabaceae↗
PubMed2005

Plant phenotypic plasticity belowground: a phylogenetic perspective on root foraging trade-offs.

Many plants proliferate roots in nutrient patches, presumably increasing nutrient uptake and plant fitness. Nutrient heterogeneity has been hypothesized to maintain community diversity because of a trade-off between the spatial extent over which plants forage (foraging scale) and their ability to proliferate roots precisely in nutrient patches (foraging precision). Empirical support for this hypothesis has been mixed, and some authors have suggested that interspecific differences in relative growth rate may be confounded with measurements of foraging precision. We collected previously published data from numerous studies of root foraging ability (foraging precision, scale, response to heterogeneity, and relative growth rate) and phylogenetic relationships for >100 plant species to test these hypotheses using comparative methods. Root foraging precision was phylogenetically and taxonomically conserved. Using a historical and phylogenetically independent contrast correlations, we found no evidence of a root foraging scale-precision trade-off, mixed support for a relative growth rate-precision relationship, and no support for the widespread assumption that foraging precision increases the benefit gained from growth in heterogeneous soil. Our understanding of the impacts of plant foraging precision and soil heterogeneity on plants and communities is less advanced than commonly believed, and we suggest several areas in which further research is needed.

Phenotype↗
PubMed2003

Precipitation pulse size effects on Sonoran Desert soil microbial crusts.

Deserts are characterized by low productivity and substantial unvegetated space, which is often covered by soil microbial crust communities. Microbial crusts are important for nitrogen fixation, soil stabilization and water infiltration, but their role in ecosystem production is not well understood. This study addresses the following questions: what are the CO2 exchange responses of crusts to pulses of water, does the contribution of crusts to ecosystem flux differ from the soil respiratory flux, and is this contribution pulse size dependent? Following water application to crusts and soils, CO2 exchange was measured and respiration was partitioned through mixing model analysis of Keeling plots across treatments. Following small precipitation pulse sizes, crusts contributed 80% of soil-level CO2 fluxes to the atmosphere. However, following a large pulse event, roots and soil microbes contributed nearly 100% of the soil-level flux. Rainfall events in southern Arizona are dominated by small pulse sizes, suggesting that crusts may frequently contribute to ecosystem production. Carbon cycle studies of arid land systems should consider crusts as important contributors because of their dynamic responses to different pulse sizes as compared to the remaining ecosystem components.

Analysis of Variance↗
PubMed1997

Gravity, light and plant form.

Plants have evolved highly sensitive and selective mechanisms that detect and respond to various aspects of their environment. As a plant develops, it integrates the environmental information perceived by all of its sensory systems and adapts its growth to the prevailing environmental conditions. Light is of critical importance because plants depend on it for energy and, thus, survival. The quantity, quality and direction of light are perceived by several different photosensory systems that together regulate nearly all stages of plant development, presumably in order to maintain photosynthetic efficiency. Gravity provides an almost constant stimulus that is the source of critical spatial information about its surroundings and provides important cues for orientating plant growth. Gravity plays a particularly important role during the early stages of seedling growth by stimulating a negative gravitropic response in the primary shoot that orientates it towards the source of light, and a positive gravitropic response in the primary root that causes it to grow down into the soil, providing support and nutrient acquisition. Gravity also influences plant form during later stages of development through its effect on lateral organs and supporting structures. Thus, the final form of a plant depends on the cumulative effects of light, gravity and other environmental sensory inputs on endogenous developmental programs. This article is focused on developmental interactions modulated by light and gravity.

Arabidopsis↗
PubMed2001

Allelopathic agents from Cyrtocymura cincta.

The sesquiterpenoid fractions from the chloroform extracts of roots and aerial parts of the weed Cyrtocymura cincta (Griseb.) H. Robinson (Compositae) were tested to observe the effects on seed germination and growth of three dicot and four monocot species. Promotion and inhibition effects were observed according to the examined compound, specific seed or cultivar, and concentration.

Argentina↗
PubMed2003

[Mechanism of gravi-sensing and -transduction in gravitropism of higher plants].

In higher plants, some organs such as roots, hypocotyls, and stems, can sense the direction of gravity to regulate their orientation. Gravitropic response is composed of four steps; 1. gravity sensing and conversion of physical stimuli to biochemical signals, 2. intracellular signal transduction in gravity sensing cells, 3. signal transmitting to responding tissues, 4. differential growth of organs. Here we focus on the former two steps. Recent studies using modern technique have gradually unveiled early events and mechanism of gravitropic response. Genetic approach provided evidences that strongly support the classical theory for gravity sensing (step 1). Computational analysis suggested the existence of another gravity sensing mechanism in roots. Spatial and temporal ion imaging in living organs in real time provided information on step 2. In addition, reverse genetic approach suggested asymmetrical intracellular distribution of auxin transporter [correction of transpoter] is a possible link between step 2 and 3. However, molecular basis of the signaling mechanism remains unknown. We believe extensive molecular genetic approach combined with recent techniques cited here shed the light to this ambiguous area of research.

Gravitation↗