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[Basic hematologic values in gilts of the Large White breed and their crosses with the Landrace breed].

The basic haematological parameters (erythrocytes, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, leucocytes, leucocyte type) were determined in 83 clinically relatively healthy gilts of the Large White breed and 85 gilts--crossbreds with the Landrace breed, at the age of five to seven months. The results were subjected to statistical processing. The erythrocyte counts are significantly higher in the crossbreds than in the gilts of the LW breed. The basic erythrocyte values, dependent on the number of erythrocytes, are significantly higher in the gilts of the Large White breed. The leucocyte counts correlate with those of the gilts of the same age; however, they are higher than in the adult sows. As a result of the evaluation of the leucocyte type, the gilts at the age of five to seven months were found to have a white blood picture of lymphocytic type. Further, a shift towards younger forms of cells of the granulocytic series was observed even in clinically relatively healthy animals.

Animals↗

Immunogenetic and population genetic analyses of Iberian cattle.

Blood samples were collected from more than 100 animals in each of 2 Spanish cattle breeds (Retinto and De Lidia), 2 Portuguese breeds (Alentejana and Mertolenga), and American Longhorn cattle. All samples for the 4 Iberian breeds were tested for 20 polymorphic systems; American Longhorn were tested for 19 of the 20. For each breed an average inbreeding coefficient was estimated by a comparison of the observed and expected heterozygosity at 7 or 8 codominant systems tested. All breeds had positive values but only 3 breeds had estimates of inbreeding that were statistically significantly different from 0: De Lidia with f = 0.17, Retinto with f = 0.08 and Mertolenga with f = 0.05. The De Lidia breed especially may be suffering from inbreeding depression since this high value is greater than expected if all of the animals were progeny of half-sib matings. Genetic distances were calculated from the gene frequency data on these 5 breeds plus 9 other European breeds. Analyses of these distances show a closely related group of the 4 Iberian breeds and American Longhorn, confirming the close relationships among the Iberian breeds and the Iberian, probably Portuguese, origin of American Longhorn cattle.

Alleles↗

Epidemiological study of blood pressure in domestic dogs.

Previous experience has shown that a non-invasive (indirect) technique using an oscillometric monitor in conjunction with a tail cuff makes routine clinical blood pressure measurement practicable in dogs. The relationship between indirect and direct readings has been evaluated in both anaesthetised and conscious dogs (Bodey and others 1994, 1996). In this study, more than 2000 pressure measurements were taken from 1903 dogs. It was found that systolic is the most variable pressure parameter and that it depends on age, breed, sex, temperament, disease state, exercise regime and, to a minor extent, diet. Diet was not a significant determinant of diastolic and mean arterial pressure. Age and breed were the major predictors for all parameters. Heart rate was primarily affected by the temperament of the animal, though other factors also play a part in prediction. The distribution of systolic, diastolic, mean arterial pressure and heart rate across the dog population approximates to a log normal distribution. On the basis of these results it is possible to describe normal ranges for canine blood pressure; definition of hypertension, though, demands attention to age and breed normal values. The existence of statistically defined hypertension in an individual or breed does not imply adverse effects justifying therapy. Among the secondary causes of hypertension, such as diabetes, obesity and hyperadrenocorticism, hepatic disease was a new addition also undocumented in humans. The hypothesis that dogs, though classic model animals for hypertension, are resistant to its development found support from the modest increase in mean pressure values observed among dogs with renal disease, notably those with substantial reduction of glomerular filtration rate. The existence of breeds such as deerhounds with average pressures in the borderline range for hypertension in humans (and many individuals, therefore, well above) suggests that dogs may also be resistant to some of the adverse effects of high blood pressure.

Age Factors↗

Heterosis, additive maternal and additive direct effects of the Red Poll and Boran breeds of cattle.

Data on 202 calves born, 191 calves weaned and 182 calves at 30 mo of age produced in 1969 from the Boran and Red Poll breeds and their reciprocal crosses were analyzed to estimate heterosis, additive maternal effects and additive direct effects on survival and growth traits from birth to 30 mo of age. The calves were produced in the Ankole District of south-western Uganda as a part of a beef cattle research program designed to provide information to support a commercial ranching development scheme in an area that had been cleared of the tsetse fly. Breed of sire effects were significant for birth and weaning weight, and breed of dam effects were significant for birth, weaning and 12-mo weight. Breed of sire and breed of dam effects that were significant showed the Boran breed to have higher values for all traits except breed of dam effects on birth weight; calves with Red Poll dams were 3.6 kg heavier (P less than .01) at birth than calves with Boran dams. Steers were significantly heavier than heifers at all ages except birth. Young cows (3 and 4 yr old) and old cows (9 yr old and older) produced calves that weighed significantly less at weaning, 12 mo and 18 mo than calves with dams that were 5 through 8 yr old. Heterosis effects were significant for weights at weaning, 12, 18, 24 and 30 mo of age; generally, heterosis effects on weight increased with age. Heterosis effects approached significance for survival from birth to 30 mo. Additive maternal effects were significant for birth weight; crossbred calves with Boran dams weighted 6.0 kg less at birth than crossbred calves with Red Poll dams. There was a reversal of the reciprocal effect between prenatal and postnatal gains; crossbred calves with Boran dams weighed 7.1 kg more at weaning than crossbred calves with Red Poll dams. Examination of additive direct effects showed the Boran breed to be significantly heavier than the Red Poll breed for birth weight and weaning weight. Additive direct effects showed the Boran breed to have generally higher (P greater than .05) values than the Red Poll breed for most other traits analyzed. Differences between straightbreds of the Boran and Red Poll breeds, which reflect differences in additive maternal and additive direct effects combined, significantly favored the Boran breed for weaning weight and 18-mo weight and favored (P less than .05) the Boran breed for all other traits except birth weight. It was concluded that the Red Poll breed had higher values for additive maternal effects for birth weight than the Boran breed in the climatic and nutritive environment in which this experiment was conducted; for most other traits evaluated, the Boran breed generally had higher values for additive maternal and higher values for additive direct effects than the Red Poll breed.

Age Factors↗

Potential improvements in rate of genetic gain from marker-assisted selection in dairy cattle breeding schemes.

The value of marker-assisted selection in dairy cattle breeding schemes is predicted by a deterministic model. In these schemes, associations between markers and milk production were assessed from production records of daughters of a grandsire by a multiple regression model with random marker effects. By tracing markers from the grandsire to grandoffspring, deviations of grandoffspring from their full-sib family mean were predicted. Predictions of the within-family variance of the grandoffspring accounted for by markers amounted to up to 13.3%. This figure decreased when the number of daughters of the grandsire analyzed decreased and, less markedly, when the distance between flanking markers increased. Prediction of within-family deviations hardly improved rates of genetic gain in conventional progeny testing schemes; equal numbers of young bulls were born annually. Genetic gain and improvement of genetic gain because of prediction of within-family deviations were much higher in nucleus schemes. In these shemes, with short optimized generation intervals, conventional selection was mainly for pedigree information and did not use the within-family variance. Analysis of highly polymorphic markers in daughters of both grandsires accounted for 4.1 to 13.3% of the within-family variance, which increased rates of gain by 9.5 to 25.8% and 7.7 to 22.4% in open and closed nucleus schemes, respectively. Risk of breeding schemes, measured by the variance of the selection response, was not increased by the use of markers.

Animals↗

[Glutathione peroxidase activity in whole blood and plasma of horses of different ages, sexes and different use].

GSH-Px activity in blood and plasma of 269 horses was determined and interrelated to age, sex, and type of use or breed. Furthermore values in blood were related to hematocrit and hemoglobin contents. Trotters and riding horses had higher GSH-Px activities in plasma as well as in blood (0.83 +/- 0.22 and 0.79 +/- 0.23 U/ml plasma or 27.2 +/- 4.3 and 24.0 +/- 7.0 U/ml blood) than Thoroughbreds in training and yearlings (0.61 +/- 0.,14 and 0.56 +/- 0.16 U/ml plasma or 20.6 2 +/- 6.9 and 24.6 +/- 4.3 U/ml blood). 3 to 6 years old Thoroughbreds had higher GSH-Px-activities than 1 to 2 years old ones. Colts and fillies did not have differing values.

Aging↗

Evaluation of mating systems involving five breeds for integrated beef production systems: II. Feedlot segment.

Computer models were used to simulate the feedlot segment of an integrated beef production system. Five breeds, Angus (A), Charolais (C), Hereford (H), Limousin (L), and Simmental (S), in three mating systems, pure-breeding and two- and three-breed rotational crossbreeding, were evaluated for feedlot and carcass performance. Breed data were taken from the literature. Feeder calves (steers and non-replacement heifers) entered the feedlot at 205 d of age. After a 35-d adjustment period, calves were custom-fed to four slaughter end points: 440 d, 457 d, 288-kg carcass weight, or low Choice. Cattle were fed to requirements (megacalories of ME). Input costs included feed and nonfeed expenses (purchase prices, transportation, yardage, and medicinal fees). Carcass values were $2.65 and $2.54/kg of carcass weight for steer and heifers, respectively, between 272 to 318 kg. Over- or underweight carcasses were discounted $.60/kg. At the 440-d end point, Select grade steer and heifer carcasses were discounted an additional $.22 and $17/kg, respectively. Biological efficiency was measured as megacalories of ME/kg of gain, and economic efficiencies were measured as input costs per kilogram of carcass weight, input costs per kilogram of lean weight, and input costs per carcass value. Continental breed combinations (C and S) were most efficient at age- and weight-constant end points for megacalories of ME per kilogram of gain and for input costs per carcass value and most efficient at all end points for input costs per kilogram of carcass weight and input costs per kilogram of lean weight. British breed combinations (A) were most efficient at a fat-constant end point for megacalories of ME per kilogram of gain and input costs per carcass value. Therefore, choosing breed combinations for feedlots depends on slaughter end point and measures of efficiency.

Adipose Tissue↗

[Values of the indices of acid-base blood homeostasis in sows of various breeds].

The indices of the acid-base homeostasis of the blood were studied in 99 sows of the Large White, English Large White, Belgian Landrace, and Hampshire breeds. The blood pH ranged from 7.32 to 7.45, and no significant differences were found between the breeds. The highest pCO2 value of 6.4 kPa was found in the LW breed; in the remaining breeds it was lower. As to base excess values, positive average values ranging from +0.3 to +1.4 mmol .1-1, were found in all the breeds. The values of the buffering stage were fairly balanced and the means ranged from 48.7 to 50.0 mmol .1-1. Balanced average values were also found in standard bicarbonate, where the values ranged from 24.6 to 25.5 mmol .1-1. Actual bicarbonate showed similar values in the Large White breed (27.3 mmol .1-1), English Large White, and Hampshire (26.2 and 26.3 mmol 1-1, respectively). In the Landrace breed the average was only 25.9 mmol .1-1. The average values of CO2 in the blood plasma ranged from 27.1 to 28.7 mmol .1-1.

Acid-Base Equilibrium↗

Aspartate aminotransferase in ejaculates of Manchego and Merino rams after minimal and maximal sperm damage.

Semen from Manchega and Merina breed rams was subjected to minimal and maximal sperm cell damage treatments. Seminal plasma samples were then analyzed for aspartate aminotransferase (AAT) activity. There were significant (P < 0.01) increases in enzymatic activity after maximal damage treatment in both breeds. Mean values of AAT activity were different between months (P < 0.01) and individual rams (P < 0.01). Breed significantly (P < 0.01) affected AAT activity after minimal and maximal sperm damage. There was an interaction (P < 0.01) between the 2 factors of ram and month in both breeds, but there was no interaction between breed and treatment.

Journal Article↗

On the weight-bearing function of the medial coronoid process in dogs.

The shape of and proportions between the surface areas of the medial coronoid process (MCP) and the fovea of the radial head were determined in 88 juvenile dogs and 146 adult dogs grouped as giant, large, mid-sized, chondrodystrophic, or small dogs. Thereby, the longitudinal (length) and transverse (width) extension of the MCP and fovea of the radial head have been measured. Original values were used to describe changes of the parameters attributed to growth. Normalized values (i.e. values expected in case of a width of the fovea of the radial head of 20 mm) were used to determine potential differences between constitutional types. All original values increased during growth (P < 0.05) except for the width and length of the MCP in chondrodystrophic and small breeds. Normalized values revealed a proportional decrease in width and length of the MCP during growth (P < 0.05) compared with the radial head. In adults, the normalized MCP was widest in giant dogs followed by large, mid-sized, small, and chondrodystrophic breeds. The MCP was also longest in giant dogs but shortest in large and chondrodystrophic dogs with those of large dogs being significantly (P < 0.05) shorter than those in giant, mid-sized and small dogs. Present results suggest that a deficiency in length-growth of the MCP--which has been present especially in large dogs--results in smaller humeral contact areas and decreased weight-bearing capacity of the MCP. Because loading forces acting on the MCP increase with body weight, the condition noted in large dogs might increase the risk of fragmentation of the MCP in these.

Aging↗

Genetic and phenotypic parameter estimates for scrotal circumference and semen traits in young beef bulls.

Estimated in this study were heritabilities and genetic and phenotypic correlations involving scrotal circumference (SC), percent live sperm, sperm number, sperm concentration, sperm motility, and an overall measure of a bull's potential breeding efficiency. Potential breeding efficiency is a composite trait based on a consideration of sperm concentration, sperm motility, sperm morphology and scrotal circumference. Data used were from three sources. Records on 863 Angus, 753 Polled Hereford, and 302 Simmental bulls were made available through the Missouri Performance-Tested Bull Sale and records on 1169 Polled Hereford bulls came from the American Polled Hereford Association. Information from these first two data sets were used to estimate heritability of scrotal circumference. The third data set was provided by Nichols Farms of Bridgewater, Iowa, and included information from the records of 465 yearling Polled Hereford and 264 yearling Simmental bulls. This latter data set was used to estimate all of the above mentioned parameters. Each data set was kept separately for the purpose of statistical analysis. Parameters were estimated using components from paternal half-sib analysis of variance and covariance. Pooled estimates of heritability for SC, sperm concentration, sperm motility, percent live sperm, sperm number and potential breeding efficiency were 0.51 +/- 0.09, 0.20 +/- 0.13, 0.11 +/- 0.12, 0.00, 0.19 +/- 0.14 and 0.13 +/- 0.12, respectively. Phenotypic correlations involving the six traits were very consistent for the two breeds. Combined across breeds their values ranged from 0.47 for SC and percent live sperm to 0.96 for sperm concentration and potential breeding efficiency. Corresponding genetic correlations were generally positive and high and ranged from 0.65 for SC and sperm motility to 1.14 for sperm number and potential breeding efficiency.

Journal Article↗

Environmental and genetic effects on preweaning growth performance of hair sheep in Mexico.

Data on 4,754 lambs born from Pelibuey (PB) and Barbados Blackbelly (BB) ewes collected over 9 years at the Mococha Experimental Centre in Yucatan, Mexico, were used to determine environmental, dam-breed and sire-breed effects on birth weight (BW), weaning weight (WW) and average daily gain (ADG). Effects of year and season of birth, sex of the lamb, type of birth, breed of dam, breed of sire, their interaction and ewe body weight were included. Heritabilities were estimated for each breed. Season of lambing was a significant source of variation on WW and ADG. Male lambs weighed more than female lambs at birth and weaning. Single born lambs were heavier than twins and triplets. Lambs born from PB dams were heavier and grew faster than those born from BB dams. Heritabilities of BW, WW and ADG for the PB breed were 0.16 +/- 0.04, 0.12 +/- 0.03 and 0.11 +/- 0.03, respectively and 0.04 +/- 0.07, 0.17 +/- 0.09 and 0.15 +/- 0.08 for the BB breed. Heterosis values were close to zero.

Animals↗

Osteological features in pure-bred dogs predisposing to thoracic or lumbar spinal cord compression.

Consistent with those vertebral sites most commonly clinically affected by spinal cord compression, body size normalised midsagittal diameters of the caudal vertebral foramen limits T10-T12 were significantly (P<0.05) lower in Dachshunds relative to other breeds. Minimal midsagittal diameters in Yorkshire Terriers and Maltese were noted at T11cd/12cr. However, these diameters were always larger (P<0.05) in small breeds compared to those in Dachshunds and large breeds suggesting that the small breeds investigated are at lower risk of developing clinical signs if a compressive disease occurs at that site. In large breeds, minimal values were present at L1cd/L2cr and in agreement with clinical findings correlate with those spinal sites most susceptible to spinal cord compression in nonchondrodystrophic large breeds. Caudal displacement of the lumbosacral enlargement of the spinal cord relative to the position previously noted in large breeds was confirmed for the Dachshunds and 50% of small breeds. However, caudal displacement was also noticed in the German Shepherd dogs.

Aging↗

[Genetic diversity and differentiation of Russian common carp (Cyprinus carpio L.) breeds inferred from RAPD markers].

Polymorphic components of the common carp Cyprinus carpio L. genome were examined by means of polymerase chain reaction with random primers (RAPD-PCR). Using four primers, genetic diversity estimates were obtained for 12 populations and seven strains of Russian common carp breeds, as well as for European Hungarian common carp and Amur wild common carp (N = 87). The highest number of polymorphic loci was revealed in Angelinskii common carp, as well as in the samples of Altai common carp and Amur wild common carp (P = 23.8-18.7%), while the lowest number of polymorphic loci was in the BB strain of Ropsha common carp. The index of genetic diversity, H, was high (11%) in Amur wild common carp, as well as in Altai and Angelinskii common carps. In the remaining breeds, the value of this index varied from 4 to 8%. Based on summarized RAPD profile (132 bands), a dendrogram of genetic differences was constructed. In this dendrogram, all breeds examined grouped into two clusters. One of the clusters was formed by Hungarian and Angelinskii common carps, and the three samples of Altai common carp. The second cluster was formed by the group consisting of the representatives of Cherepetskskii, Stavropol, and Ropsha common carps, along with the differing from them Amur wild common carp. The observed differentiation was confirmed by the analysis of the polymorphic markers variance by the method of principle components. Evolutionary history and the reasons for genetic differentiation of Russian common carp breeds are discussed.

Animals↗

Milk progesterone in postpartum and pregnant cows as a monitor of reproductive status.

Milk samples were analyzed for progesterone content by a petroleum ehter extraction and competitive protein binding assay validated for milk. In one experiment, 11 cows were sampled twice daily for 24 days beginning with an observed estrus 15 to 45 days postpartum, and again 19, 21, 23, and 25 days after breeding. Progesterone values during the estrous cycle paralleled those for blood plasma but were slightly higher at estrus (1.49 ng/ml milk) and maximum (9 ng/ml) on days 11 to 16 of the estrous cycle. After breeding, cows later diagnosed pregnant averaged 7.12 ng/ml while those later found to be nonpregnant averaged 2.36 ng/ml. All diagnoses of pregnancy were correct. In a separate experiment there was no difference between milk from front and rear quarters, but progesterone was highest in last milk, intermediate in composite milk, and lowest in first milk.

Animals↗

Pleuritis secondary to pneumonia or lung abscessation in 90 horses.

Of 122 horses with pleural effusion, 90 (73.8%) had pleuritis secondary to pneumonia or lung abscessation. Fifty-one horses died or were euthanatized. The highest prevalence was in Thoroughbred and Standardbred racehorses. Eleven (12.2%) horses were postsurgical patients and 22 (24.4%) horses had been transported over 500 miles. There was no relationship between final outcome and the age, sex, breed, hematologic values, or laboratory findings pertaining to pleural fluid except for the bacterial isolation of Escherichia coli from the pleural fluid, as this was more frequently associated with death. Follow-up on 38 of the 39 horses that survived showed that 18 (46.2%) recovered and were able to return to performance equal to that prior to their illness. Ten (25.6%) were returned for breeding or pleasure use, with no attempt made to return them to racing. Follow-up was not available for 5 horses, 4 horses had just recently been discharged from the hospital, and 2 horses are racing poorer than prior to their illness.

Animals↗