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PubMed1992

Hoffmann half-frame external fixation rigidity and its relationship to universal joint slippage.

Hoffmann half-frame external fixation device configurations often fail under minimal loads secondary to joint slippage. In these experiments improved universal joints that were developed in an earlier study were tested on Hoffmann half-frame assemblies. The rigidity of selected half-frame configurations was tested in four modes (axial compression, torsion, medial-lateral and anterior-posterior four-point bending). These results were compared to those of an earlier, similar study looking at the standard Hoffmann half-frame. No changes in overall rigidity were noted, but significant increases in yield loads and loads to frame failure were achieved. Such improvements will increase the reliability and usefulness of the Hoffmann device to the orthopaedic community.

Equipment Design↗

PubMed2025

CompareM2 is a genomes-to-report pipeline for comparing microbial genomes.

SUMMARY: Here, we present CompareM2, a genomes-to-report pipeline for comparative analysis of bacterial and archaeal genomes derived from isolates and metagenomic assemblies. CompareM2 is easy to install and operate, designed in such a way that the user can install the complete software in one step and launch all analyses on a set of microbial genomes (bacterial and archaeal) in a single action. The central results generated via the CompareM2 workflow are emphasized in a portable dynamic report document. AVAILABILITY AND IMPLEMENTATION: CompareM2 is a free software that is scalable to a range of project sizes, and welcomes modifications and pull requests from the community on its Git repository at https://github.com/cmkobel/comparem2.

Software↗

PubMed2025

The genome sequence of the White-barred Clearwing, Synanthedon spheciformis (Denis & Schiffermüller), 1775 (Lepidoptera: Sesiidae).

We present a genome assembly from a female specimen of Synanthedon spheciformis (White-barred Clearwing; Arthropoda; Insecta; Lepidoptera; Sesiidae). The assembly contains two haplotypes with total lengths of 388.57 megabases and 372.40 megabases. Most of haplotype 1 (99.88%) is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 19.11 kilobases.

Keywords: Synanthedon spheciformis↗

PubMed2025

The genome sequence of the Garden Tiger, Arctia caja (Linnaeus, 1758) (Lepidoptera: Erebidae).

We present a genome assembly from a male specimen of Arctia caja (Garden Tiger; Arthropoda; Insecta; Lepidoptera; Erebidae). The assembly contains two haplotypes with total lengths of 700.59 megabases and 699.20 megabases. Most of haplotype 1 (99.63%) is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.41 kilobases.

Arctia caja↗

PubMed2025

The genome sequence of the Dusky Grizzled Skipper, Pyrgus cacaliae (Rambur, 1839) (Lepidoptera: Hesperiidae).

We present a genome assembly from a female specimen of Pyrgus cacaliae (Dusky Grizzled Skipper; Arthropoda; Insecta; Lepidoptera; Hesperiidae). The assembly contains two haplotypes with total lengths of 808.81 megabases and 696.79 megabases. Most of haplotype 1 (94.63%) is scaffolded into 31 chromosomal pseudomolecules, including the W and Z sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.43 kilobases.

Dusky Grizzled Skipper↗

PubMed2025

The genome sequence of the Large Wall Brown, Lasiommata maera (Linnaeus, 1758) (Lepidoptera: Nymphalidae).

We present a genome assembly from a female specimen of Lasiommata maera (Large Wall Brown; Arthropoda; Insecta; Lepidoptera; Nymphalidae). The assembly contains two haplotypes with total lengths of 480.89 megabases and 424.47 megabases. Most of haplotype 1 (99.88%) is scaffolded into 29 chromosomal pseudomolecules, including the W and Z sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.77 kilobases.

Large Wall Brown↗

PubMed2001

Burkholderia cepacia lower respiratory tract infection associated with exposure to a respiratory therapist.

OBJECTIVE: To investigate and control a nosocomial outbreak of Burkholderia cepacia lower respiratory tract infection. DESIGN: Outbreak investigation and case-control study. SETTING: A 260-bed community hospital. PATIENTS: Participants were mechanically ventilated intensive care patients without cystic fibrosis. A case was defined as a hospitalized patient with a sputum culture positive for B. cepacia between January 1 and November 6, 1998. METHODS: Respiratory therapy infection control policies and practices were reviewed; laboratory and environmental studies and a retrospective case-control study were conducted. Case-patients were matched with control-patients on age, gender, diagnosis, and type of intensive care unit. RESULTS: Nine case-patients were identified; B. cepacia likely caused pneumonia in seven and colonization in two. Two respiratory therapy practices probably contributed to the transmission of B. cepacia: multidose albuterol vials were used among several patients, and nebulizer assemblies often were not dried between uses. B. cepacia was grown from cultures of three previously opened multidose vials; pulsed-field gel electrophoresis patterns of B. cepacia from seven case-patients and two multidose vials were indistinguishable. Case-patients had longer durations of heated humidified mechanical ventilation (mean, 9.8 days vs 4.4 days; P=.03) and were more likely to have exposure to one particular respiratory therapist than controls (odds ratio, undefined; 95% confidence interval, 4.7-infinity; P=.001). The association with the respiratory therapist, a temporary employee, persisted after controlling for duration of heated humidified ventilation. No new B. cepacia infections were identified after control measures were implemented. CONCLUSIONS: B. cepacia probably was transmitted among patients through use of extrinsically contaminated multidose albuterol vials. Respiratory therapy departments must pay close attention to infection control practices, particularly among new or temporary staff.

Aged↗

PubMed2003

Centrosome and spindle pole body dynamics. Review and abstracts of the EMBO/EMBL Conference on Centrosomes and Spindle Pole Bodies, Heidelberg, September 13-17, 2002.

Five years after the first meeting held on Centrosomes and Spindle Pole Bodies, a second meeting was organized by Tano Gonzalez, Eric Karsenti, Kip Sluder, and Mark Winey in Heidelberg, Germany. Sponsored by the gracious European community (EMBO/EMBL), the meeting was both spectacular and exhausting. The wealth of information delivered, the plethora of model systems and unique approaches described, and the free exchange of information by a cooperative and excited community of scientists overwhelmed all participants. Even the best prepared scholars could not have anticipated the avalanche of data and insights that poured from the presentations from beginning to end. Daily posters by young and senior scientists added dimension to round out the well-planned series of presentations. The meeting began with opening remarks by Eric Karsenti and Michel Bornens who reminded participants of the historical questions of the field. Where does the centrosome come from? What are the mechanisms that control centrosome assembly and duplication? How is duplication coordinated with the cell cycle? Why do some cells have centrosomes, while others do not? What are the components of the centrosome? Does the centrosome play an important role in disease?

Animals↗

PubMedDate not supplied

The cognitive profile of anorexia nervosa: a comparative study including a community-based sample.

A community-based sample of adolescent-onset anorexia nervosa (AN) cases (n = 51) was contrasted with an age-, sex-, and school-matched comparison group [comp] (n = 51) on the Wechsler Adult Intelligence Scale-Revised (WAIS-R) at a mean age of 21 years. There were no study dropouts. Fewer than 10% of AN cases were underweight at the time of testing. Overall, there were few differences across the two groups, even though the COMP group performed significantly better on the object assembly subtest. A small subgroup of AN cases showed autism-spectrum disorders. This subgroup tended toward test profiles similar to those observed in autism and Asperger syndrome. These findings are discussed in relation to the clinical need for subgrouping of AN cases with a view to improving treatment programs/interviews.

Adolescent↗

PubMed2001

Hospital readmission: predicting the risk.

This approach focused on identifying specific variables that predict the likelihood of readmission. It involved clinical, utilization, and demographic variables that are generally available on hospital computer abstract databases. The approach included a process for identifying and comparing individual variables with the highest risk of readmission. It also contained a procedure for assembling risk populations including combinations of variables. The approach demonstrated the potential for using risk analysis to maximize the focus of clinical management on patient outcomes while reducing the amount of resources required for this process.

Adult↗

PubMed1991

Integrated maps of the Drosophila genome: progress and prospects.

A physical map of the Drosophila melanogaster genome is being assembled, consisting of ordered overlapping cosmid clones. The map is constructed in steps, separately for each chromosomal division. Gaps in this map are to be bridged with yeast artificial chromosome clones. Hybridization to previously cloned genes and extensive use of in situ hybridization to polytene chromosomes ensure that the cosmid map is firmly anchored to the wealth of available genetic and cytogenetic information. The intention is to make the physical map widely available as part of an overall, integrated genetic resource for the Drosophila research community.

Animals↗

PubMed2006

Stability of the EasyOne ultrasonic spirometer for use in general practice.

OBJECTIVE AND BACKGROUND: Spirometry is recommended for the diagnosis and management of chronic respiratory diseases in the community. Spirometer accuracy is critical, but few general practitioners meet the American Thoracic Society and European Respiratory Society (ATS/ERS) recommendation for daily calibration. The aim of this study was to assess the accuracy and stability of a portable ultrasonic spirometer (EasyOne) that the manufacturer claims does not require regular calibration. METHODS: Six EasyOne spirometers were used in a practice-based spirometry study. Inspiratory and expiratory accuracy was checked periodically using a certified 3-L syringe. Paired calibration checks were performed using a dedicated mouthpiece assembly (spirette) and randomly selected spirettes. RESULTS: The six spirometers were used for up to 26 weeks (mean 23.9 weeks) and a total of 1041 spirometry tests and 75 syringe calibrations were performed. All inspiratory and expiratory calibration checks using a dedicated or randomly selected spirette met the ATS/ERS accuracy criterion (3.00+/-0.105 L). The mean (range) expiratory volume deviation from target volume (3.00 L) was 0.011 L (-0.06-0.09 L) using a dedicated spirette and 0.046 L (-0.09-0.10 L) using randomly selected spirettes. The deviation from target was not affected by the mean flow generated during the calibration procedure. There was no change in calibration during the study. CONCLUSIONS: This study supports the manufacturer's claim that the EasyOne spirometer maintains its calibration during routine clinical use in general practice and does not require daily calibration as specified in international spirometry guidelines.

Equipment Design↗

PubMed1997

Effect of acute hyperglycemia on colorectal motor and sensory function in humans.

Increased use of laxatives and constipation are more common among people with diabetes mellitus than matched nondiabetic people in the same community. The mechanism of constipation in diabetes is unclear. Acute hyperglycemia was previously reported to reduce the gastrocolonic response. Our aim was to determine the effects of acute hyperglycemia on the colon compliance and motor response to feeding and on the sensory function of the colon and rectum in healthy human subjects. Eleven healthy individuals were studied under conditions of hyperglycemia (mean blood glucose 280 +/- 13 mg/dl) and euglycemia. We evaluated three parameters: 1) colonic motility and compliance by a multilumen manometry and barostatic balloon assembly in the descending colon (motility was studied during fasting and for 2 h postprandially); 2) perception of isobaric distensions of polyethylene balloons in the rectum and colon; and 3) rectal compliance. Initial tonic response to meal ingestion (0-5 min) was slightly lower during hyperglycemia (P = 0.3). However, colonic tone, motility, compliance, and sensation, as well as rectal compliance and sensation, were not significantly different under the conditions of euglycemia and acute hyperglycemia. In healthy individuals, acute hyperglycemia does not significantly change colonic or rectal motor functions or the perception of mechanosensory stimuli in the colon or rectum compared with euglycemia. These results do not support the hypothesis that hyperglycemia abolishes the colonic response to feeding.

Adult↗

PubMed2025

The genome sequence of the de Prunner's Ringlet, Erebia triaria von Prunner, 1798 (Lepidoptera: Nymphalidae).

We present a genome assembly from a female specimen of Erebia triaria (de Prunner's Ringlet; Arthropoda; Insecta; Lepidoptera; Nymphalidae). The assembly contains two haplotypes with total lengths of 521.30 megabases and 412.03 megabases. Most of haplotype 1 (99.7%) is scaffolded into 17 chromosomal pseudomolecules, including the Z 1, Z 2, and W sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.26 kilobases.

Erebia triaria↗

PubMed2002

Osmotic adaptation in yeast--control of the yeast osmolyte system.

The yeast Saccharomyces cerevisiae (baker's yeast or budding yeast) is an excellent eukaryotic model system for cellular biology with a well-explored, completely sequenced genome. Yeast cells possess robust systems for osmotic adaptation. Central to the response to high osmolarity is the HOG pathway, one of the best-explored MAP kinase pathways. This pathway controls via different transcription factors the expression of more than 150 genes. In addition, osmotic responses are also controlled by protein kinase A via a general stress response pathway and by presently unknown signaling systems. The HOG pathway partially controls expression of genes encoding enzymes in glycerol production. Glycerol is the main yeast osmolyte, and its production is essential for growth in a high osmolarity medium. Upon hypo-osmotic shock, yeast cells transiently stimulate another MAP kinase pathway, the so-called PKC pathway, which appears to orchestrate the assembly of the cell surface and the cell wall. In addition, yeast cells show signs of a regulated volume decrease by rapidly exporting glycerol through Fps1p. This unusual MIP channel is gated by osmotic changes and thereby plays a key role in controlling the intracellular osmolyte content. Yeast cells also possess two aquaporins, Aqy1p and Aqy2p. The production of both proteins is strictly regulated, suggesting that these water channels play very specific roles in yeast physiology. Aqy1p appears to be developmentally regulated. Given the strong yeast research community and the excellent tools of genetics and functional genomics available, we expect yeast to be the best-explored cellular organism for several years ahead, and osmotic responses are a focus of interest for numerous yeast researchers.

Adaptation, Physiological↗

PubMed2025

The genome sequence of the Titania's Fritillary, Boloria titania (Esper, 1793) (Lepidoptera: Nymphalidae).

We present a genome assembly from a female specimen of Boloria titania (Titania's Fritillary; Arthropoda; Insecta; Lepidoptera; Nymphalidae). The assembly contains two haplotypes with total lengths of 496.64 megabases and 454.08 megabases. Most of haplotype 1 (97.13%) is scaffolded into 32 chromosomal pseudomolecules, including the W and Z sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.17 kilobases.

Boloria titania↗

PubMed2026

The genome sequence of the Red-underwing Skipper, Spialia sertorius (Hoffmannsegg, 1804) (Lepidoptera: Hesperiidae).

We present a genome assembly from a female specimen of Spialia sertorius (Red-underwing Skipper; Arthropoda; Insecta; Lepidoptera; Hesperiidae). The assembly contains two haplotypes with total lengths of 364.57 megabases and 323.91 megabases. Most of haplotype 1 (99.92%) is scaffolded into 32 chromosomal pseudomolecules, including the W and Z sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.34 kilobases. Gene annotation of this assembly on Ensembl identified 12,823 protein-coding genes.

Lepidoptera↗

PubMed2026

The genome sequence of the Southern Grizzled Skipper, Pyrgus malvoides (Elwes & Edwards, 1897) (Lepidoptera: Hesperiidae).

We present a genome assembly from a male specimen of Pyrgus malvoides (Southern Grizzled Skipper; Arthropoda; Insecta; Lepidoptera; Hesperiidae). The assembly contains two haplotypes with total lengths of 746.71 megabases and 747.11 megabases. Most of haplotype 1 (99.8%) is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.41 kilobases. Gene annotation of this assembly on Ensembl identified 14,715 protein-coding genes.

Lepidoptera↗