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PubMed1976

Artificial insemination using homologous semen: a review of 158 cases.

One hundred fifty-eight women underwent artificial insemination with homologous semen (AIH) in an attempt to achieve conception. Only 15 (9.5%) were successful. Women with anatomical abnormalities were not excluded from the study and they were less successful than the normal women, but results were disappointing in both groups. The most frequently recorded indication for AIH was decreased density or motility of the husband's sperm, but pregnancy occurred in only 2 of the 48 cases in which sperm count was consistently less than 50 X 10(6)/ml and in only 3 of the 63 cases in which sperm motility was consistently less than 60%. When several semen analyses revealed considerable fluctuation in semen quality, the chances for impregnation by natural means appeared to be greater than the likelihood of success with AIH. The procedure does not seem to compensate for diminished count or motility, does not seem to be of particular value in cases of unexplained infertility, and appears to be indicated only in very special cases, if at all.

Adult↗

PubMed1999

Transmission of classical swine fever virus by artificial insemination.

Classical swine fever (CSF) virus was introduced into an artificial insemination centre during the CSF epizootic of 1997-1998 in the Netherlands. The risk of further spread of CSF virus via contaminated semen was recognised, but could not be assessed because scientific data on this issue were not available. An animal experiment was performed to determine whether CSF virus could be transmitted via artificial insemination with contaminated semen. Three boars were inoculated with a CSF virus field isolate and from Day 5 till Day 18 thereafter, ejaculates were collected and prepared for insemination. Ruttish sows were inseminated with the extended semen from Day 5 till Day 18 after inoculation of the boars. All the inoculated boars remained healthy throughout the experiment and developed CSF neutralising antibodies between 14 and 21 days after inoculation. Virus was isolated from several semen samples collected from 5 till 11 days after inoculation. Two out of six sows inseminated with CSF contaminated semen seroconverted after insemination. All the other sows remained seronegative. In the foetuses of both the seropositive sows, CSF virus was detected at approximately 35 days post insemination. These results demonstrate that adult boars infected with CSF virus can excrete virus with semen and can, subsequently, transmit the virus to sows and their foetuses via artificial insemination.

Animals↗

PubMed1984

[Psychosocial situation of married couples prior to artificial insemination with donor sperm].

57 couples who attended our outpatient department for artificial insemination with donor sperm (AID) had a detailed semistandardized interview prior to treatment. The Giessen Test was used as personality test, as well as a questionnaire concerning the sexual and partner relationship. We found that it was the woman who had to bear the major part of the intrapsychic burden and who primarily suffered because of the problem which was actually caused by male sterility. However, 54 of 57 couples had no psychological difficulties to overcome the sterility problem. These couples developed individual and partnership strategies to deal with the burden of unwanted childlessness. They preferred AID rather than adoption, because for the woman this alternative offers the chance of real parenthood. AID seems to provide a medico-technical solution for couples with sterility problems: the reproductive process at the biological level was regarded as distinctly separated from their future role as parents which they considered to be a social task.

Adult↗

PubMed1990

Karyotype screening of potential sperm donors for artificial insemination.

Cytogenetic studies were carried out in 100 potential semen donors for artificial insemination (AI) before they underwent the routine procedures for acceptance or rejection into the programme, namely medical history, physical examination and blood and semen analyses. Results were only compared at the end of the study. In 80 cases, the karyotype was normal; 12 males showed polymorphic chromosome variants; seven had pericentric inversions of heterochromatic regions; one had a short inversion of chromosome 2; and in one case centromere fragility was observed. Six of the 12 males with normal variants were accepted into the programme and four of them had fathered from one to 13 normal children at the end of the study; the other six had been rejected, four of them because of abnormal seminograms, and another two because the motility control of the frozen semen was negative. Of the seven males with pericentric inversions, one dropped out of the programme; four were accepted and three of them had produced from two to five normal children at the end of the study; two had been rejected due to abnormal seminograms. The individual with centromere fragility was accepted and had produced four normal children at the end of the series. Our conclusion is that although cytogenetic studies of potential donors for AI would be desirable, routine screening for chromosome anomalies is not justified at present.

Genetic Testing↗

PubMedDate not supplied

Factors affecting the success of artificial insemination by frozen donor semen.

The records of 227 couples who received artificial insemination by frozen donor semen in 984 treatment cycles were analysed to find out the factors affecting the pregnancy rates. The cumulative pregnancy rate at 6 months was 46.8% and the pregnancy rate per cycle was 10%. The pregnancy rates were adversely affected by (1) increase in the recipient age above 30 years, (2) irregularity of menstrual cycles, (3) the use of clomiphene citrate for induction of ovulation, and (4) low cervical mucus scores. Among our donor semen samples selected with a set of criteria, the sperm counts and initial and post-thaw motility did not affect the pregnancy rates.

Adult↗

PubMedDate not supplied

[Results of heterologous artificial insemination, ethical- deontological implications].

Fourteen female patients married to infertile men were artificially inseminated with the semen of unknown donors within 1985-1987. Over 150 inseminations (no complications except sporadic pain) produced 7 pregnancies out of which 5 were finished with the delivery of healthy babies, one was terminated in the seventh week with abortion. Fate of the remaining pregnancy is unknown. Ethical-legal implications of AID, the problem of donors and classification of semen, and necessity to store the semen at the temperature of liquid nitrogen due to the risk of AID are emphasized.

Abortion, Spontaneous↗

PubMed1988

[Donor artificial insemination. Psychological survey of 830 couples].

830 couples who had asked for Artificial Insemination by Donor (AID) were questioned using separate questionnaires for the husband and for the wife. The questions were directed to the reactions that followed in succession to discovery of the sterility, the psychological conditions that led to choosing AID, their attitude as far as secrecy was concerned and finally their contribution to finding donors of sperm. Overall the husband-wife replies corresponded to one another. Frequently the reaction to the news that the man is sterile is a depressive one and to a lesser degree followed by troubles in sexual performance. The choice of AID is usually a decision of both members of the couple. Most couples express themselves as hesitant about adoption. Most of them said that secrecy about the procedure was an essential condition. It appears that, above all, male sterility had to be hidden from the circle in which they associated and from the child. It is this position as far as secrecy is concerned that makes it difficult for the couples to help in recruiting sperm donors.

Attitude↗

PubMedDate not supplied

Cervical cap homologous artificial insemination (AIH): the University of Florence experience.

We report our experience with artificial insemination with husband's semen (AIH) using cervical cap. Forty-four couples were admitted to our AIH program over a two-year period. In 4 couples, AIH was performed due to impotence or hypospadia, while in the remaining 40 couples indications for AIH were abnormal semen, poor post-coital test, anti-sperm antibodies, poor cervical mucus. Overall, six pregnancies were obtained (pregnancy rate = 15%) in the latter group; in particular, AIH was able to elicit pregnancy in 5 out of the 25 cases of abnormal semen (20%). Our data emphasize the importance of female factors associated with infertility since significantly higher pregnancy rates (45.4% versus 0%) were observed in subjects in whom these factors were absent.

Adult↗

PubMed1983

Some attitudes to artificial insemination by donor.

A preliminary survey to assess and quantify attitudes to Artificial Insemination by Donor (AID) has been conducted with the aid of an article in a national magazine. The consensus of 375 individuals (mostly with a vested interest in infertility) concerning their attitudes to semen donation, hypothetical receipt of donor semen, legal aspects and the keeping of donor records, etc., have been analysed and their answers correlated to their age, sex, ethnic grouping, educational level, religion, and marital and fertility status as well as their place of residence. Within the limitations of this study, three major conclusions can be drawn. Firstly there are probably many potential semen donors in the general community and recruitment techniques need to be improved to capitalize on this source. Secondly, a centralized non-identifying National Register of donor information may be tolerated, and finally, the couples questioned felt an AID child should not be likened to one received through adoption. These and other findings would suggest that attitudes towards AID are changing and consequently some of the practices within AID programmes may need to be altered. Further statistically conducted surveys are warranted to determine these current attitudes.

Adult↗

PubMed1982

A preliminary psychosocial follow-up of parents and their children conceived by artificial insemination by donor (AID).

Forty-three couples who first conceived by artificial insemination by donor (AID) between 1975-1977 jointly completed an anonymous questionnaire about their attitudes to the AID programme. There were 80 children, and 30 couples had 2 or more children by AID. All children developed normally. The attendance rate of husbands at the births was 94%. All but one couple reported stable marriages and the majority thought that the AID pregnancies had improved their marital relationship, and none thought it had deteriorated. Selection for treatment relied upon the traditional medical interview only and no structured psychosocial interviews were used. The majority of the couples (84%) did not plan to tell their children of their AID origins.

Australia↗

PubMed1992

Artificial insemination in swine.

This article analyses the advantages and disadvantages of artificial insemination with semen purchased from a center as well as from the herd boars on the farm. Intensive swine production could benefit greatly by adapting artificial insemination with herd boars, particularly from savings in labor and boar numbers. The techniques for semen collection, extension, and insemination are described, and sources for equipment given. Expected results of artificial insemination are quoted from experiments and international field experience.

Animal Husbandry↗

PubMed2006

Artificial insemination in domestic cats (Felis catus).

Artificial insemination (AI) in cats represents an important technique for increasing the contribution of genetically valuable individuals in specific populations, whether they be highly pedigreed purebred cats, medically important laboratory cats or endangered non-domestic cats. Semen is collected using electrical stimulation, with an artificial vagina or from intact or excised cauda epididymis. Sperm samples can be used for AI immediately after collection, after temporary storage above 0 degrees C or after cryopreservation. There have been three and five reports on intravaginal and intrauterine insemination, respectively, and one report on tubal insemination with fresh semen. In studies using fresh semen, it was reported that conception rates of 50% or higher were obtained by intravaginal insemination with 10-50x10(6) spermatozoa, while, in another report, the conception rate was 78% after AI with 80x10(6) spermatozoa. After intrauterine insemination, conception rates following deposition of 6.2x10(6) and 8x10(6) spermatozoa were reported to be 50 and 80%, respectively. With tubal insemination, the conception rate was 43% when 4x10(6) spermatozoa were used, showing that the number of spermatozoa required to obtain a satisfactory conception rate was similar to that of cats inseminated directly into the uterus. When frozen semen was used for intravaginal insemination the conception rate was rather low, but intrauterine insemination with 50x10(6) frozen/thawed spermatozoa resulted in a conception rate of 57%. Furthermore, in one report, conception was obtained by intrauterine insemination of frozen epididymal spermatozoa. Overall, there have been few reports on artificial insemination in cats. The results obtained to date show considerable variation, both within and among laboratories depending upon the type and number of spermatozoa used and the site of sperm deposition. Undoubtedly, future studies will identify the major factors required to consistently obtain reliable conception rates, so that AI can become a practical technique for enhancing the production of desirable genotypes, both for laboratory and conservation purposes.

Animals↗

PubMed1994

Assessing the viability of mutant and manipulated sperm by artificial insemination of Caenorhabditis elegans.

We describe a protocol for artificial insemination of Caenorhabditis elegans which we used to evaluate the viability of sperm from different strains and of sperm activated in vitro. Worms can be artificially inseminated with almost 100% success. Both male and hermaphrodite sperm can be used for insemination. Sperm from a sterile hermaphrodite [fem-3(q23ts)] were found to be viable. As with normal mating, male sperm inseminated into hermaphrodites artificially outcompete the hermaphrodite's own sperm, even though they have not been ejaculated with seminal fluid. Spermatozoa that were activated in vitro from spermatids by the weak base triethanolamine were viable. In contrast, spermatozoa activated in vitro by protease treatment were not.

Animals↗

PubMed1990

Human immunodeficiency virus transmission through artificial insemination.

Six human immunodeficiency virus type 1 (HIV-1) seropositive semen donors were identified during an antibody screening program initiated at an artificial insemination clinic in New York City in 1985. A total of 176 women underwent artificial insemination with fresh semen from these six donors between 1978 and mid-1985. Of these 176 women, 134 women were HIV-1 antibody tested and 1 was found to be seropositive; she denied all other AIDS-associated risk behavior and her husband was seronegative. The results of this investigation provide additional evidence that HIV-1 transmission can occur through artificial insemination with semen from an infected donor although the risk of HIV-1 transmission appears to be lower than that reported by other investigators. It is clear that HIV-1 screening of all potential semen donors should be routine practice whenever artificial insemination is performed.

Acquired Immunodeficiency Syndrome↗

PubMed1980

Artificial insemination with husband's semen: prognostic factors.

This retrospective review of 38 couples has considered 148 cycles of artificial insemination with husband's semen (AIH) and 178 cycles of natural insemination with husband's semen (NIH). The indication for AIH in every case was that postcoital testing had revealed less than 15 motile sperm/high-power field of endocervical mucus. Conception through AIH occurred in 12 cases; 1 couple conceived by NIH. The rate of conception per cycle was significantly higher in the AIH cycles than in the NIH cycles (P < 0.005). No man with both low density (less than 25 million spermatozoa/ml) and low motility (less than 50%) on semen analysis produced a pregnancy through AIH. Couples demonstrating more than 15 motile sperm/high-power field (on examination of cervical mucus 2 hours after AIH) were more likely to conceive through AIH than couples demonstrating less than 15 motile sperm. The role of AIH is discussed.

Coitus↗

PubMed1993

Vaginal anatomy of the domestic cat (Felis catus) in relation to copulation and artificial insemination.

In the course of an investigation of artificial insemination (AI) in the domestic cat, the site of deposition of the semen during vulval insemination was questioned. A limited study was carried out to ascertain both the anatomy and distensibility of the vagina and urogenital sinus, and the location of the cervix in relation to the vulva. In live restrained female cats a 4 mm diameter Teflon probe could not be inserted more than 20 mm, and elicited a cry similar to the copulatory vocalization when the resistance was encountered. However, a 1 mm diameter ball-tipped needle was passed to a depth of 40 mm. To confirm these observations, four adult female domestic cats were dissected. The cervix was located approximately 45 mm cranial to the vulva. The urogenital sinus narrowed approximately 17.5 mm from the vulva, preventing the passage of a 4 mm probe, and led to a constricted vagina, which permitted the passage of a 1 mm diameter probe to the depth of the cervix. A radiological investigation of a single anaesthetized cat supported these results. The erect penis of the adult male was measured during sexual arousal in five animals; the mean length and width (+/- SD) was 21.2 +/- 2.2 mm and 5.1 +/- 0.5 mm, respectively. These observations lead us to conclude that the stimulus for vocalization during natural mating is distention of the posterior vagina, where semen is probably deposited. The site of semen deposition is uncertain in several accounts of artificial insemination, as the passage of the insemination device depends on its diameter.

Animals↗

PubMed1981

Tubal patency testing in a programme of artificial insemination with donor semen.

The value of investigating tubal patency by hysterosalpingography, before artificial insemination with donor semen (AID), was studied retrospectively in 167 patients. The results suggest that unless there is a history of pelvic inflammatory disease preliminary hysterosalpinography is unnecessary. Conception rates were similar in women who had a normal hysterosalpingogram (44%), in those who had an abnormal hysterosalpingogram (46%), and in those who had no preliminary tubal investigation (39%) before AID. Life table analysis of expectancy of conception suggests that tubal patency testing should be delayed until after AID has been unsuccessful for four cycles. Laparoscopy is then the most worthwhile investigation.

Fallopian Tube Patency Tests↗

PubMed1984

Estimation of fertility and fecundity in women receiving artificial insemination by donor semen and in normal fertile women.

The pregnancy rates after artificial insemination by donor semen (AID) have been compared with pregnancy rates in normal fertile women to assess the efficiency of AID. To do this, the curve y = a(1-(1-b)x) was fitted to life-tabled cumulative pregnancy rates. The equation describes a model in which the parameter a is the proportion of women who are potentially fertile under the conditions of treatment, and in which the parameter b is the pregnancy rate per cycle (or fecundity) of these fertile women. For 259 AID patients with no previous pregnancy a was 65% while for 57 AID patients with a previous pregnancy after AID 'a' was 99.9%. The values of b were similar for the two groups of patients, being 20% and 22% respectively. Women without fertility problems who had become pregnant after discontinuing oral contraception provided the reference group. Since only pregnant women were selected, a was 100% by definition. The values of b for the reference group were 22% for 100 primigravid women and 20% for 100 multigravid women. Only 65% of the AID patients were potentially fertile with AID, but those that were fertile became pregnant at the same rate as normal women who discontinued oral contraception.

Adult↗