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Results for “LYMPHOSARCOMA”

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At least 559 records · Page 31Linked to original sources

PubMed1993

Phenotype and ontogeny of cells carrying a tumor-associated antigen that is expressed on bovine leukemia virus-induced lymphosarcoma.

The phenotype and ontogeny of cells carrying the tumor-associated antigen (TAA), identified in tumors of cattle with enzootic bovine leukosis (EBL) by use of the monoclonal antibody (MAb) c143, were analyzed by flow cytometry and immunohistochemistry. The TAA recognized by the c143 MAb (c143 TAA) was mainly expressed on B-cells, macrophages, reticular cells, and a minor population of BoCD4-positive T-cells in bovine leukemia virus (BLV)-free normal cattle. When the peripheral blood mononuclear cells from normal cattle were activated in vitro, some populations of BoCD8-positive T- and non-T/non-B-cells also showed expression. Moreover, B-cells expressed the c143 TAA until the antigen was lost from cells at the final stage of B-cell differentiation, namely, the plasma cell stage. The c143 MAb-positive cells in blood of BLV-free normal cattle form heterologous subpopulations, and these cells coexpress other surface markers such as BoCD2, BoCD5, BoCD6, and B-cell-specific molecules B1 low, B1 bright, and B2. In BLV-infected cattle, the proportion of peripheral blood mononuclear cells that express the c143 TAA increased with the progression of EBL, and, in addition, BLV-infected cattle that had no lymphosarcomas showed increased proportions of c143 MAb-positive cells that coexpressed surface IgM (sIgM), BoCD5, B1 low, and B2 but not BoCD2, BoCD4, or BoCD6. Furthermore, most of the c143 MAb-positive tumors from all cattle with EBL appeared to be a monoclonal population derived from a single B-cell and to be divided into two types, c143 TAA+BoCD5+B1 low+ B2+ sIgM+ or sIgM-. Collectively, these results show that the c143 TAA is not only a useful surface marker for identification of EBL but also a marker of differentiation of lymphoid cells.

Animals↗

PubMed1993

Spinal lymphosarcoma and disseminated mastocytoma associated with feline immunodeficiency virus infection in a cat.

The course of naturally acquired infection with feline immunodeficiency virus was monitored in a cat over an 18-month period after diagnosis. The cat was admitted with diarrhea, poor body condition, a bite wound abscess, gingivitis, chronic fever, and splenomegaly. The cat's condition improved after splenectomy and remained stable for approximately 15 months, then began to deteriorate, as gingivitis, polyuria, polydipsia, pyrexia, multiple cutaneous masses, and hind limb paresis developed. The in vitro response of the cat's lymphocytes to mitogens was suppressed, and absolute lymphocyte counts were low. Spinal lymphosarcoma, disseminated mastocytoma, and presumptive diabetes mellitus were diagnosed after euthanasia. Decreased immune surveillance associated with feline immunodeficiency virus-related immunosuppression possibly played a role in the development of neoplastic disease in this cat.

Animals↗

PubMed1993

[Lymphosarcoma in a horse].

A sixteen-year old pregnant Dutch Warmblood mare was referred to the Faculty of Veterinary Medicine because of sluggishness since eight days. The main clinical features were depression, pale mucous membranes with petechiae, ventral oedema and fever. Haematological evaluation revealed severe anaemia (haematocrit 0.15 L.L.-1) and leukopenia (2.3 G.L-1 with 96% lymphocytes). There was no thrombocytopenia. The horse died following a hospitalisation period of six days. At necropsy a (multicentric) lymphosarcoma was found in the bone marrow with neoplastic infiltration of the kidneys.

Anemia↗

PubMed1996

Probable recurrent femoral artery thrombosis in a dog with intestinal lymphosarcoma.

An 11-year-old mixed-breed dog was examined because of chronic diarrhea, anorexia, and weight loss. Clinicopathologic abnormalities included anemia and hypoalbuminemia, and protein-losing enteropathy was identified. Acute, unilateral, femoral artery thrombosis developed before the cause of the protein-losing enteropathy could be identified. The dog was treated with aspirin, and sensation and function of the affected limb returned over the next 5 days, but thrombosis of the opposite femoral artery then developed. The dog was euthanatized, and at necropsy, intestinal lymphosarcoma was the only disease process found. Although disseminated intravascular coagulation is a well-recognized potential complication of neoplasia in dogs, recurrent localized thrombosis, as in this dog, also can develop.

Animals↗

PubMed1996

The response of mouse lymphosarcoma cells to continuous gamma irradiation.

Long-lasting survival of LS/BL tumor cells under continuous exposure in vivo to 60Co irradiation at an exposure rate of 10 cGy/h was observed. While LS/BL tumor cells tolerated the irradiation for more than 150 weeks, the number of hematopoietic stem cells decreased to less than 1% of control values within 7 days of irradiation. The liver colony method was used to develop sublines from nonirradiated and continuously (110 weeks) irradiated LS/BL cells for the analysis of karyotypes of the resulting clones of lymphosarcoma cells. The appearance of new structural abnormalities t(12:9) in one of the sublines tested correlated with its faster host killing when compared to the parent line.

Animals↗

PubMed1996

Immunohistochemical identification of B and T lymphocytes in formalin-fixed, paraffin-embedded feline lymphosarcomas: relation to feline leukemia virus status, tumor site, and patient age.

The lymphocyte phenotype of 70 formalin-fixed, paraffin-embedded feline lymphosarcomas (LSAs) was determined immunohistochemically using a T cell polyclonal antibody, and a B cell monoclonal antibody. Forty-seven of 70 (67%) tumors were T cell, 19/70 (27%) were B cell, and 4/70 (6%) did not stain with either marker. Thirty-eight of 70 (54%) tumors were positive for feline leukemia virus (FeLV) antigen by immunohistochemistry (IHC), and 52/70 (74%) tumors were positive for FeLV DNA using the polymerase chain reaction (PCR). B cell tumors were as frequently FeLV-positive as T cell tumors using either IHC or PCR. Intestinal tumors were more likely to be B cell than T. The incidence of B and T cell tumors was not different among young (< or = 3 y), middle-aged (> 3 y to < or = 8 y), and old (> 8 y) cats. Both B and T cell tumors from old cats were FeLV-positive more often by PCR than by IHC. Feline leukemia virus DNA but not antigen, was detected in B cell tumors and intestinal tumors from cats > 8 y as often as it was detected in B cell tumors and intestinal tumors from cats < or = 8 y. Previously, most B cell and intestinal tumors from old cats were considered to be negative for FeLV. Here, the results suggest involvement of latent or replication-defective forms of the virus in such tumors from old cats. This study supports a role for FeLV in feline B cell as well as T cell tumorigenesis.

Aging↗

PubMedDate not supplied

Lymphosarcoma with plasmacytoid differentiation in a scarlet macaw (Ara macao).

A lymphosarcoma in a scarlet macaw (Ara macao) affecting periocular structures is described. Microscopically and ultrastructurally, many of the lymphoid cells had plasmacytoid features. Polymerase chain reaction amplification failed to detect exogenous avian retrovirus RAV-1 in the neoplastic mass.

Animals↗

PubMed1997

Adoptive immunotherapy of murine lymphosarcoma: comparative study using allo-immune or tumor-immune T cells.

The antitumor effect of allosensitization with lymphocytes and skin graft of DBA/2 mice was evaluated using immunogeneic, transplantable Lymphosarcoma (LS-A) syngeneic to Swiss mice. A dose dependent tumor inhibitory effect in terms of tumor free mice was observed in mice sensitized i.p. with lymphocyte doses between 10-100 million per animal. Sensitization with allogeneic primary skin graft was more effective than lymphocyte immunization. The antitumor immunity could be adoptively transferred in syngeneic Swiss mice using either allo-immune or tumor-immune T cells. Analysis of T cell phenotypes using monoclonal antibodies against cell surface markers CD4 and CD8, indicated absolute dependence on the CD4+ T cells subset in tumor cure in case of allo-immune as well as tumor-immune T cells. CD8+ T cell subset was found essential only in case of allo-immune T cell therapy. Immunosuppression of mice with whole body gamma irradiation (4 Gy), 6 hr before transfer of allo-immune or tumor-immune T cells did not abrogate the therapeutic ability of allo-immune or tumor-immune T cells. Our results suggest that allosensitization could be an effective method of generating effector lymphocyte populations that might be used to treat tumors that exhibit detectable immunogenecity.

Animals↗

PubMed1997

[Diagnostic criteria and prognosis of mediastinal lesions in children with lymphosarcoma].

Treatment for mediastinal lymphosarcoma was given to 71 patients, aged 3-14 years, at the Institute's Clinics during 1982-1991. In that group, there were more males than females (3.4:1), mean duration of the condition of 3 months, T-cell immunity pattern (89.9%), enlarged anterior mediastinal lymph nodes and thymus, pleural lesions including pleuritis, mean mediastinal-thoracic index of 0.5, compression syndrome, elevated concentration of lactate dehydrogenase and presence of tumor cells in the bone marrow and peripheral blood. Among major negative factors of prognosis were inadequate therapy, pleural lesions and stage IV tumor.

Adolescent↗

PubMed1996

Phytohemagglutinin-induced gut hyperplasia and the growth of a mouse lymphosarcoma tumor.

NMRI mice injected subcutaneously with Krebs II lymphosarcoma cells and fed on a diet containing the kidney bean lectin phytohemagglutinin (PHA) within the range 0.45-7.0 mg/g diet, developed tumors during a 10 day period which on average were only 35% of the dry weight of tumors in lactalbumin (La) fed mice (control). The reduction in growth occurred in a dose-dependent manner in the range 0.45-3.5 mg/g diet. The degree of hyperplasia of the small intestine in response to feeding the PHA diets was higher in non-injected compared to injected mice. A lipolytic effect of PHA was observed above 1.75 mg/g diet in control mice and the highest concentration had a major effect on body weight. Since the index of hyperplasia at the lowest PHA concentration tested did not correlate with the reduction in tumor size, it is suggested that other factors in addition to the initial lectin-induced gut hyperplasia are involved in slowing down the progression of tumor growth.

Animals↗

PubMed1976

Cell membrane sialoglycopeptides of corticoid-sensitive and -resistant lymphosarcoma P1798.

Cells of corticoid-sensitive (CS) and corticoid-resistant (CR) lymphosarcoma P1798 were labeled in vivo with either [14C]- or [3H]fucose before and after treatment with 9-alpha-fluoroprednisolone (9-FP). Labeled glycopeptides, derived from isolated, Pronase-digested cell membranes of both tumors were separated by gel filtration on Bio-Gel P-10 by a double-label technique. Elution profiles of CS and CR fractions showed significant differences in early eluting material. Desialylation of glycopeptides by neuraminidase lowered the molecular weight of both CS and CR fractions, and altered 3H:14C ratios indicated that CS and CR sialoglycopeptides are different. 9-FP treatment for 7 hr increased the density of isolated P1798-CS and -CR cell membranes. All CS and CR glycopeptides from treated tumors eluted faster than did those of untreated preparations. Both CS and CR sialoglycopeptides were altered, although differences in CS and CR profiles persisted. Histochemical investigations indicated that negative charge, present on surfaces of untreated CS cells, is lost between 6 and 8 hr after exposure in vivo to 9-FP. CR cells had no or few anionic sites on their surfaces before and after steroid administration. We demonstrated that glycopeptides of both CS and CR tumors contain sialic acid, although only CS cells carry a surface-exposed negative charge that is lost after 9-FP treatment. Glucocorticoids alter both P1798-CS and -CR sialoglycopeptides, but the consistent differences between their chromatographic patterns suggest that steroid-induced changes in cell membranes of the two tumors are not identical. Cell death or survival of glucocorticoid-treated P1798 cells may, therefore, be influenced by specific structural characteristics involving cell surface sialoglycoproteins.

Alcian Blue↗

PubMed1976

Lymphosarcoma in a rabbit.

Generalized lymphosarcoma was observed in a 7-month-old New Zealand White rabbit. Numerous grayish white nodules were found in the kidney and liver. The mucosal surface of the stomach was hemorrhagic and ulcerated. Virus-like particles were observed in neoplastic tissue from the kidney on electron microscopic examination. Significance of the presence of virus-like particles was not determined.

Animals↗

PubMed1976

[Effect of preparations of messenger RNA from the spleens of immunized rats on antibody synthesis in rat lymphosarcoma].

Messenger RNA was isolated from spleen polysomes of rats immunized with sheep red cells, two methods being employed: a sorption of mRNA on on membrane filters, and poly(U)-cellulose affinity chromatography. The rat transplantable lymphosarcoma cells treated with this RNA started hemolysine synthesis which persisted throughout many cell generations in transplantation of the tumor. The probability is discussed of m-RNA not only functioning as a template for antibody synthesis but also deblocating appropriate genes in recipient cells.

Animals↗

PubMed1953