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PubMedDate not supplied

Separation of gametocytes of Leucocytozoon smithi from whole peripheral turkey blood.

A technique that uses a Ficoll density-gradient system was devised for isolating gametocytes of the parasitic blood protozoan Leucocytozoon smithi from whole turkey blood. Turkey blood containing gametocytes was layered onto a 35% solution of Ficoll and centrifuged. A white band, containing gametocytes and leukocytes, was observed just below the Ficoll-plasma interphase, clearly separated from the underlying packed erythrocytes. The gametocyte-leukocyte suspension was then subjected to a second centrifugation on a continuous gradient of Ficoll ranging from 12% at the top of the tube to 35% at the bottom. The second centrifugation separated the white cells containing the gametocytes into 5 distinct layers: 1) thrombocytes; 2) gametocytes of L. smithi; 3) lymphocytes; 4) a band of monocytes; and 5) granulocytes. Recovered gametocytes, stored at 4 C in a sterile balanced salt solution, retained their morphological integrity for a minimum of 4 weeks.

Animals↗
PubMed1999

Administration of diminazene aceturate or imidocarb dipropionate for treatment of cytauxzoonosis in cats.

Bobcats (Lynx rufus) are the reservoir hosts for Cytauxzoon felis, the causative agent of cytauxzoonosis. Cytauxzoonosis is a highly fatal tickborne blood protozoal disease of domestic and exotic cats. Treatment of clinically affected cats has generally been unrewarding. In our report, 6 of 7 cats had signs of illness and laboratory findings indicative of cytauxzoonosis and were successfully treated with 2 i.m. injections of diminazene aceturate or imidocarb dipropionate (2 mg/kg [0.9 mg/lb] of body weight, each). One cat died after the first injection of diminazene. Additional treatment with isotonic fluids i.v. and heparin s.c. were used as supportive care for dehydration and disseminated intravascular coagulation that were detected by laboratory testing between diminazene or imidocarb treatments. Atropine was effective in recovery and preventing adverse reactions associated with imidocarb treatment of 1 cat.

Animals↗
PubMed1975

Dogs and intestinal parasites: a public health problem.

The stools of 239 stray dogs were examined for intestinal parasites. Of the helminths found, Toxocara canis (43.5%), tapeworms (25.5%), Ascaris species (21.3%) and hookworms (12.5%) were the commonest. Of the protozoans found, Isospora species and Entamoeba coli were the most prevalent. An unusual feature of the present study was the finding of Ascaris species. The importance of the high prevalence of intestinal parasites in dogs, the close contact of humans with dogs' excreta and the possible role of this environmental pollution in the spread of human disease are discussed.

Animals↗
PubMed2002

Pfiesteria complex organisms and human illness.

BACKGROUND: Pfiesteria piscicida and look-alike dinoflagellates are collectively known as Pfiesteria complex organisms (PCOs). The purposes of this paper are to evaluate the threat that PCOs pose to human health and to clarify some common misconceptions concerning these organisms. METHODS: References about P piscicida and PCOs were identified and reviewed using MEDLINE, Sea Grant databases, and other sources obtained from various Internet sites. RESULTS: Researchers exposed to aerosols of water containing P piscicida in a laboratory setting exhibited a variety of symptoms, including reversible cognitive impairment and loss of short-term memory. This led to multiple biologic, animal model, and epidemiologic studies that are reviewed and discussed. CONCLUSIONS: Exposure to P piscicida and possibly other PCOs can cause human illness. Until further isolation and purification of toxins from PCOs allows for development of tests to detect these toxins, predicting and documenting toxic PCO fish kills as well as potential PCO-related illness will be difficult.

Animals↗