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At least 577 records · Page 32Linked to original sources
PubMed1985

A chronic technique for high frequency blood sampling/transfusion in the freely behaving rat which does not affect prolactin and corticosterone secretion.

A blood sampling/blood transfusion technique is described for chronically cannulated freely behaving rats. The procedure permits high frequency sampling for several hours at a maximum rate of one blood sample per min. Prolactin and corticosterone were used as indicators of stress. In male rats the prolactin concentration in blood obtained by rapid decapitation was not significantly different from that obtained through a cannula. A blood volume reduction of 1 or 2 ml did not affect prolactin or corticosterone secretion; however, a reduction of 3 ml or more increased corticosterone secretion but did not consistently increase prolactin secretion. When blood volume reduction was compensated for by blood transfusion, frequent blood sampling did not affect prolactin or corticosterone secretion in dioestrous rats. The surges of prolactin secretion during the afternoon of pro-oestrus and pseudopregnancy were also unaffected by high frequency blood sampling. It is therefore concluded that the blood sampling/transfusion procedure described does not stress the animals. Its advantages include not only the possibility of following individual hormone profiles, but also the economic and ethical aspects of reducing the number of animals needed for experimentation.

Animals↗
PubMed2001

Transfusion medicine : support of patients undergoing cardiac surgery.

There is still no alternative that is as effective or as well tolerated as blood; nevertheless, the search for ways to conserve, and even eliminate blood transfusion, continues. Based on hemoglobin levels, practice guidelines for the use of perioperative transfusion of red blood cells in patients undergoing coronary artery bypass grafting have been formulated by the National Institutes of Health and the American Society of Anesthesiologists. However, it has been argued that more physiologic indicators of adequacy of oxygen delivery should be used to assess the need for blood transfusion. Methods used for conserving blood during surgery include autologous blood donation, acute normovolemic hemodilution and intra- and postoperative blood recovery and reinfusion. The guidelines for the use of autologous blood transfusion are controversial and it does not appear to be cost effective compared with allogeneic blood transfusion in patients undergoing cardiac surgery. Similarly, the cost effectiveness of intra- and postoperative blood recovery and reinfusion need further evaluation. Treatment with recombinant human erythropoietin (rhEPO) remains unapproved in the US for patients undergoing cardiac or vascular surgery, but it is a valuable adjunct in Jehovah's Witness patients, for whom blood is unacceptable. The characterization of darbepoetin alfa, a novel erythropoiesis stimulating protein with a 3-fold greater plasma elimination half-life compared with rhEPO, is an important advance in this field. Darbepoetin alfa appears to be effective in treating the anemia in patients with renal failure or cancer and trials in patients with surgical anemia are planned. Desmopressin has been used to effectively reduce intraoperative blood loss. Topical agents to prevent blood loss, such as fibrin glue and fibrin gel, and agents that alter platelet function, such as aspirin (acetylsalicylic acid) or dipyridamole, need further evaluation in patients undergoing cardiac surgery. Aprotinin has been shown to preserve hemostasis and reduce allogeneic blood exposure to a greater extent than the antifibrinolytic agents tranexamic acid and aminocaproic acid. Controlled clinical trials comparing the costs of these agents with clinical outcomes, along with tolerability profiles in patients at risk for substantial perioperative bleeding are needed.

Blood Loss, Surgical↗
PubMed1979

Effect of acetylsalicylic acid, paracetamol and placebo on pain and blood loss in dysmenorrheic women.

The analgesic effect of paracetamol, acetyl-salicylic acid, and placebo on dysmenorrhea were compared in a double-blind cross-over study of 30 women. There was a moderate placebo effect, but no significant difference was found between the three treatments. Blood loss was also measured and it did not vary, with the type of drug ingested. It is concluded that paracetamol and acetylsalicylic acid in the doses used (0.5 g x 4 for 3 days) were not effective against heavy dysmenorrhea, and that none of the drugs influenced the amount of blood lost.

Acetaminophen↗
PubMed1982

Management of pregnancies with rare blood types.

Pregnant women with antibodies to high incidence blood group antigens should be diagnosed as early as possible and red blood cells should be stored frozen for future possible needs. Either autologous, compatible siblings or unrelated individuals' red blood cells should be frozen. The application of such a program is described in three pregnant women with exceedingly rare antibodies to high incidence blood group antigens (anti-Lutheranb, and PP1Pk, and anti c with blood type B CCddee Du negative).

Adult↗
PubMed1978

Investigation of relationship between lecithin:cholesterol acyltransferase activity and coronary atheroma, diagnosed by selective coronary angiography.

140 consecutive patients undergoing selective coronary angiography in the investigation of chest pain had fasting blood specimens collected. 24 specimens were unsuitable due to hemolysis or to incomplete coronary angiography studies. In the 116 remaining patients the serum was analyzed for total cholesterol, free cholesterol, and the initial rate of cholesterol esterification. LCAT activity was determined both as the percentage esterification per minute, and as nmols/ml/min. All the coronary angiograms were examined by a single cardiologist who was unaware of the results of the serum tests. Fixed predetermined criteria were used, resulting in all patients being classified as one of four groups. 36 patients had no detectable atheroma, 23 mild, 21 moderate, and 36 severe. Statistical analysis revealed a significant relationship between the presence and severity of coronary atheroma with the patient's age, and an equally strong relationship was found with the patient's total serum cholesterol. No statistically valid correlation could be found between LCAT activity (as determined by esterification in the patient's native serum) and atheroma. Free cholesterol levels also did not correlate with the presence of antheroma. An interesting finding was the presence of severe antheroma in a small number of patients with low normal level of serum cholesterol and high LCAT activity.

Angiography↗
PubMed1980

Diagnosis, size estimation and prediction of acute myocardial infarction from S-myoglobin observations. A system analysis to assess the influence of various sources of variability.

Three different ways of using S-myoglobin observations for early diagnosis of acute myocardial infarction have been investigated by computer simulation techniques, viz. classification based on (i) single determination in relation to a decision limit, (ii) the peak serum concentration value, (iii) estimated or predicted value of infarct size from serial serum concentration determinations. The results of the in numero experiments indicate that it is possible to define optimal conditions with regard to time, period and frequency of observation, as well as to assess requirements on pre-analytical/analytical variation. The optimal time for a single observation should be about 10-12 h after onset of symptoms. The influence of pre-analytical/analytical variation is not very critical in this connection and the quality requirements are achievable in the clinical chemical laboratory today (total CV about 0.10). The peak serum value has good diagnostic power, but does not provide a good index of infarct size, no matter how good the analytical quality may be. It should be possible to predict infarct size from early serial S-myoglobin observations. A coefficient of pre-analytical/analytical variation below 0.05 is then required in addition to frequent blood specimen collection from admission up to peak time of the serum concentration curve.

Humans↗
PubMed1984

Bacterial shock due to transfusion with Yersinia enterocolitica infected blood.

A fatal case (a 55-yr-old man) of bacterial shock and sepsis following a transfusion with erythrocytes infected with Yersinia enterocolitica serotype 03, is reported. The blood donor had slight diarrhea 6 days before the blood donation. A serum sample from the donor showed high titre of both IgG and IgM antibodies against Y. enterocolitica 03, indicating a recent infection. Y. enterocolitica 03 was isolated from blood cultures from the patient. The remaining portion of the transfused erythrocyte concentrate also yielded abundant growth of the same organism on direct plating of the material on blood agar indicating that profuse multiplication of the organism had occurred within the transfusion bag during storage at 4 degrees C.

Blood Donors↗
PubMed1986

Circadian and diurnal variation of circulating immune complexes, complement-mediated solubilization, and the complement split product C3d in rheumatoid arthritis.

Nine patients with active classical rheumatoid arthritis (ARA criteria) were studied with reference to circadian variation of immunological and clinical parameters. Complement-mediated solubilization (CMS) of immune complexes (IC) and the level of circulating IC were found to be inversely related with low CMS and increased IC levels in the morning, and vice versa in the afternoon. Bed rest and exercise did not influence these fluctuations. The C3d concentration in plasma was increased but showed no diurnal or circadian periodic fluctuations when the levels were corrected for fluctuations in plasma albumin concentration. Clinical assessment by means of pain score exhibited marked variations, with high scores in the morning, and lower in the daytime, whereas measurements of Ritchie's joint index showed no consistent pattern. The circadian variations in CMS, serum IC and clinical parameters indicate the need to collect blood specimens and perform clinical examinations of patients at a fixed time of day.

Adult↗
PubMed1990

Percutaneous umbilical blood sampling.

Percutaneous umbilical blood sampling (PUBS), also called cordocentesis, is a newly introduced technique that enables blood samples to be obtained from the fetus in utero for a variety of conditions. The major applications are for the diagnosis of fetal infections, karyotype analysis, fetal growth retardation, diagnosis of hematologic conditions, and metabolic evaluation. This procedure is gaining in popularity, since it provides direct information on fetal blood status. It can be applied to therapeutic manipulations such as in utero transfusions or drug administration. The procedure is remarkably safe and has few technical problems. The applicability of its use in the assessment of fetal thrombocytopenia is also discussed in detail.

Blood Specimen Collection↗
PubMed1993

Use of finger-prick human blood samples as a more convenient way for in-vitro screening of modified hemoglobin blood substitutes for complement activation: a preliminary report.

Safety test of modified hemoglobin in animals does not always reflect safety in human. We have earlier reported an in-vitro preclinical screening test based on in-vitro complement activation of human plasma In this test, modified hemoglobin is added to human plasma in a test tube. Complement activation is followed by the C3a levels. Since this directly measures the effect of modified hemoglobin on human plasma, this could be an important bridge before clinical use in patients. The use of plasma is suitable for research, development and industrial applications. However, there are many extra steps involved and may not be convenient for population or patient screening on a large scale bases. The present study shows that it is possible to use small sample of whole blood obtained directly from finger pricks and use immediately for analysing complement activation using an ELISA enzyme immunoassay method.

Blood Specimen Collection↗
PubMed1984

Blood constituents in lactating Holstein cows influenced by hematocrit, sampling site, and diet protein and calcium.

Constituents were measured in jugular vein (days 0, 4, 14, 28, 43, 72, and 151 of lactation) and internal iliac artery and mammary vein (days 28, 72, and 151 of lactation) blood of 24 Holsteins. Six diets of grain:corn silage-urea contained percents of protein and calcium: 12, .6; 12, .9; 15, .6; 15, .9; 15, .6; 15, .9. Grain contained urea (diets 3, 4) or soybean meal (diets 5, 6). All cows were fed diet 3 the first 4 wk of lactation; then four cows were assigned to each of six diets. In jugular blood, calcium and phosphorus were lower on day 0. Hydroxyproline and ketone bodies peaked and magnesium was minimal on day 4. Hematocrit, urea nitrogen, and free fatty acids decreased with lactation. Jugular blood from younger cows had less ketone bodies, free fatty acids, and magnesium and more hematocrit, hydroxyproline, and phosphorus. Sampling site had arterial-venous differences for each constituent, with venous differences for hematocrit, free fatty acids, calcium, phosphorus, and magnesium. Hematocrit decreased with diets 1, 2, 3, 4, or .6% calcium. With a common hematocrit (36%) and change from baseline (day 28), urea nitrogen was less with 12% protein or .6% calcium. Phosphorus and magnesium decreased with 15% protein. More constituents were required from diet and tissues to maintain concentrations in blood as hematocrit decreased, indicating the importance of dietary calcium and protein concentration and quality.

Age Factors↗
PubMed1999

Prevalence of enteric hepatitis A and E viruses in the Mekong River delta region of Vietnam.

A study of antibody prevalence for hepatitis A virus (HAV) and hepatitis E virus (HEV) was carried out in southwestern Vietnam in an area adjacent to a known focus of epidemic HEV transmission. The purpose of this investigation was first to provide a prevalence measure of hepatitis infections, and second to determine the outbreak potential of HEV as a function of the susceptible population. Blood specimens collected from 646 persons in randomly selected village hamlets were examined by an ELISA for anti-HEV IgG and anti-HAV IgG. The prevalences of anti-HEV IgG and anti-HAV IgG were 9% and 97%, respectively. There was a significant increase (P < 0.01) in age-specific anti-HEV IgG. A notable increase in anti-HAV IgG prevalence (P < 0.0001) occurred between child populations 0-4 (64%) and 5-9 (95%) years of age. No evidence of familial clustering of anti-HEV IgG-positive individuals was detected, and household crowding was not associated with the spread of HEV. Boiling of water was found to be of protective value against HEV transmission. A relatively low prevalence of anti-HEV indicates considerable HEV outbreak potential, against a background of 1) poor, water-related hygiene/sanitation, 2) dependence on a (likely human/animal waste)-contaminated Mekong riverine system, and 3) periodic river flooding.

Adolescent↗