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Surveillance and control of infectious diseases at local, national and international levels.

New emerging and re-emerging threats, the weight of public opinion and new technology for surveillance and treatment are likely to impact on how, and if, effective surveillance can be performed in the future. If surveillance fails to address the needs of practitioners and policy-makers, it is likely that there will be loss of confidence. Current surveillance systems are reasonably effective at detecting significant events that are localised in time and space. It is more difficult to detect diffuse and progressive events with a slow increase over time or sporadic and widespread events without obvious links to time, place or person. Detection of these events relies on good data collection, comparative background data and sophisticated analytical tools. To improve surveillance systems, we need methods with the appropriate sensitivity and specificity for the outputs desired. Targeted surveillance should enable better ascertainment of those cases which must be considered and those which can be dismissed. New methods, such as mathematical modelling and geographical information systems, support conventional surveillance in moving events into the known and predictable category. It is important to integrate surveillance across local, regional and international levels and to base surveillance on local public health structures. The purpose and value of data aggregation at each level and the amount of detail needed at each level must be carefully evaluated. The key to all these improvements is developing the workforce. Surveillance needs individuals with a broad range of skills: clinical, epidemiological, anthropological, and mathematical; in particular, people who can think laterally. These individuals must be encouraged through effective training courses, good mentorship, networking and clear career structures.

Communicable Disease Control↗

Reliable measures of behaviorally-evoked cardiovascular reactivity from a PC-based test battery: results from student and community samples.

This paper describes efforts to reduce measurement error in the assessment of cardiovascular reactivity by standardizing task requirements and by aggregating data across tasks and testing sessions. Using these methods, reliable measures of reactivity (.80 or greater) were obtained on five different measures of cardiovascular function (heart rate, systolic blood pressure, diastolic blood pressure, stroke volume, pre-ejection period) in samples of college students and community volunteers. Methodological limitations may have hampered previous efforts in this area. Current findings are consistent with a dispositional model of cardiovascular reactivity, and they suggest productive future strategies for obtaining reliable assessments.

Adult↗

Assessment of maternal mortality in Tanzania.

OBJECTIVE: To assess maternal mortality. DESIGN: Prospective community-based survey, a sisterhood method survey and hospital data. SETTING: The study was performed in communities in a rural area in Northwestern Tanzania and the local district hospital. RESULTS: Four hundred and forty-seven pregnant women in the community survey were followed up as far as six weeks after delivery; there were 2865 respondents in the sisterhood survey; the hospital study involved 7526 deliveries. Maternal morality ratios derived from the prospective community-based survey, the sisterhood method survey and hospital data analysis were 241, 297, and 845 per 100,000 live births, respectively. CONCLUSIONS: Hospital data tend to overestimate maternal mortality in these communities. The sisterhood method is easier and more convenient than a prospective community-based study.

Adolescent↗

Social sciences research: ethical and policy implications.

Ethical issues are raised about the conduct of social research in the dental field particularly with respect to the use of survey methodology and aggregated data. Problems associated with respondent identifiers, open-ended and probing questioning, privacy of subject-matter, community contamination and burden, group stereotyping, knowledge of law violations, mis-use of data banks, re-use of data, effects of disclosure, and referral for treatment are discussed. The natural and the contrived social experiments are reviewed as well as the issue of needed research on the effects of regulation on science and on the protection of privacy.

Behavioral Research↗

A statewide school tobacco policy review: process, results, and implications.

The federal Pro-Children's Act of 1994 and other public health laws prompted most schools to create policies that address tobacco issues. To date, however, the literature is devoid of research that assesses the quality of a district's tobacco policy. This article describes the process and results from a large-scale, tobacco policy review. An Interagency Tobacco Task Group requested that tobacco policies of all New York State Schools be reviewed. A policy rubric was developed using the documents Fit, Healthy, and Ready to Learn and the School Health Index. The rubric assessed five policy components. Aggregate data were calculated for each policy component and for the final score. School policies also were evaluated for compliance with state and federal laws. Overall policy review scores were quite low, suggesting greater effort be placed on helping schools to develop more effective tobacco policies. Initial efforts included sending letters and a "tobacco tool kit" to schools containing recommendations and resources to improve their district's tobacco policy.

Adolescent↗

SNOMED representation of explanatory knowledge in veterinary clinical pathology.

BACKGROUND: The Systematized Nomenclature of Medicine (SNOMED) is an established standard nomenclature for the expression of human and veterinary medical concepts. Nomenclature standards ease sharing of medical information, create common points of understanding, and improve data aggregation and analysis. OBJECTIVES: The objective of this study was to determine whether SNOMED adequately represented concepts relevant to veterinary clinical pathology. METHODS: Concepts were isolated from 3 different types of clinical pathology documents: 1) a textbook (Textbook), 2) the Results sections of industry pathology reports (Findings), and Discussion sections from industry pathology reports (Discussion). Concepts were matched (mapped) by 2 reviewers to semantically-equivalent SNOMED concepts. A quality score of 3 (good match), 2 (problem match), or 1 (no match) was recorded along with the SNOMED hierarchical location of each mapped concept. Results were analyzed using Cohen's Kappa statistic to assess reviewer agreement and chi-square tests to evaluate association between document type and quality score. RESULTS: The percentage of good matches was 48.3% for the Textbook, 45.4% for Findings, and 47.5% for Discussion documents, with no significant difference among documents. Of remaining concepts, 40% were partially expressed by SNOMED and 14% did not match. Mean reviewer agreement on quality score assignments was 76.8%. CONCLUSIONS: Although SNOMED representation of veterinary clinical pathology content was limited, missing and problem concepts were confined to a relatively small area of terminology. This limitation should be addressed in revisions of SNOMED to optimize SNOMED for veterinary clinical pathology applications.

Information Storage and Retrieval↗

The relationship between secretory immunity, mood and life-events.

Twelve subjects volunteered to take part in a short trial involving daily life-event and mood recording over a period of up to two weeks. On each day subjects also provided timed saliva samples. Aggregated data across the trial period revealed that unstimulated secretion rate of secretory immunoglobulin A from whole saliva correlated strongly and significantly with net desirable event reporting, defined as a subject's tendency to report relatively frequent desirable events and relatively infrequent undesirable events. Correlations with positive and negative mood were insignificant, although the pattern of results was in line with hypotheses. Within-subject analyses revealed a totally contrary pattern of results. In particular, negative mood was significantly associated with higher sIgA secretion rate. Analyses involving total sIgA concentration paralleled those using secretion rate. Results are discussed in relation to psychoneuroimmunological models of illness vulnerability, particularly upper respiratory infection, and previous findings in regard to secretory immunity.

Adult↗

Mood states and minor illness.

Mood and health reports from 65 administrative and clerical staff were obtained daily over a period of several weeks. Three mood factors emerged from the aggregated data which appear to be most suitably labelled: happiness, tense depression and hostile depression. Subjects high on hostile depression suffered more from colds. Subjects scoring highly on tense depressed mood reported more insomnia, head and neck aches. The results, especially in regard to hostile depression, are discussed in a wider context, including possible overlap with core affective aspects of the well-known Type A coronary risk construct. Finally, psychoimmunological interpretations of the link between hostile depression and vulnerability to colds are considered.

Adult↗

Resources, population, environment: an oversupply of false bad news.

False bad news about population growth, natural resources, and the environment is published widely in the face of contradictory evidence. For example, the world supply of arable land has actually been increasing, the scarcity of natural resources including food and energy has been decreasing, and basic measures of U.S. environmental quality show positive trends. The aggregate data show no long-run negative effect of population growth upon the standard of living. Models that embody forces omitted in the past, especially the influence of population size upon productivity increase, suggest a long-run positive effect of additional people.

Agriculture↗

A diffusible compound can enhance conjugal transfer of the Ti plasmid in Agrobacterium tumefaciens.

Several octopine strains of Agrobacterium tumefaciens were tested for Ti plasmid (pTi) transfer after induction by 400 micrograms of octopine per ml for 24 h. The strains could be divided into two groups, transfer efficient (Trae) and transfer inefficient (Traie); the respective rates of transfer were 0.77 x 10(-2) to 1.14 x 10(-2) and 0.33 x 10(-6) to 9.8 x 10(-6) plasmid transconjugant per donor cell. Transfer efficiencies of Traie strains were greatly increased when the time of induction was 72 h. A diffusible conjugation factor (CF) that can enhance conjugal transfer of pTi in A. tumefaciens was discovered when both Trae and Traie donor strains were induced in the same plate. The evidence indicates that CF is a key factor affecting transfer efficiency of pTi but is not sufficient by itself to induce transfer. Trac mutants can produce CF constitutively, and Trae strains can produce it after induction by low octopine concentrations. The transfer efficiency of Traie strains was greatly increased by adding CF to the induction medium. The thermosensitive strain B6S, which normally cannot conjugate at temperatures above 30 degrees C, could transfer pTi efficiently at 32 and 34 degrees C in the presence of CF. Production of CF is dependent on the presence of pTi but appears to be common for different opine strains; it was first detected in octopine strains, but nopaline strains also produced the same or a similar compound. CF is very biologically active, affecting donor but not recipient bacterial cells, but CF does not promote aggregation. Data suggest that CF might be an activator or derepressor in the conjugation system of A. tumefaciens. CF is a dialyzable small molecule and is resistant to DNase, RNase, protease, and heating to 100 degrees C for 10 min, but autoclaving (121 degrees C for 15 min) and alkaline treatment removed all activity.

Arginine↗

Mechanism of activation of cytochrome C peroxidase activity by cardiolipin.

In this work, the actions of bovine heart cardiolipin, synthetic tetraoleyl cardiolipin, and a nonspecific anionic detergent sodium dodecyl sulfate (SDS) on cytochrome c (Cyt c) peroxidase activity recorded by chemiluminescence in the presence of luminol and on the Fe...S(Met80) bond whose presence was estimated by a weak absorption band amplitude with peak at 695-700 nm (A(695)) were compared. A strict concurrency between Fe...S(Met80) breaking (A(695)) and cytochrome peroxidase activity enhancement was shown to exist at cardiolipin/Cyt c and SDS/Cyt c molar ratios of 0 : 1 to 50 : 1 (by chemiluminescence). Nevertheless, when A(695) completely disappeared, Cyt c peroxidase activity under the action of cardiolipin was 20 times more than that under the action of SDS, and at low ligand/protein molar ratios (=4), SDS failed to activate peroxidase activity while cardiolipin enhanced Cyt c peroxidase activity 16-20-fold. A(695) did not change on Cyt c binding with liposomes consisting of tetraoleyl cardiolipin and phosphatidylcholine (1 : 10 : 10), while peroxidase activity was enhanced by a factor of 8. Breaking of 70% of the Fe...S(Met80) bonds resulted in only threefold enhancement of peroxidase activity. Cardiolipin-activated Cyt c peroxidase activity was reduced by high ionic strength solution (1 M KCl). The aggregated data suggest that cardiolipin activating action is caused, first, by a nonspecific effect of Fe...S(Met80) breaking as the result of conformational changes in the protein globule caused by the protein surface electrostatic recharging by an anionic amphiphilic molecule, and second, by a specific acceleration of the peroxidation reaction which is most likely due to enhanced heme accessibility for H(2)O(2) as a result of the hydrophobic interaction between cardiolipin and cytochrome.

Animals↗

A special conditions register.

A special conditions register (SCR) linked to the child health system's register of all children has been in use in West Sussex since 1977. This paper describes the aims, organisation, and use of the SCR and gives examples of the aggregated data that may be obtained. Of the 155,000 children aged 0-17 resident in West Sussex in 1990, 4.3% were included on the SCR. Altogether 45.7% of children on the SCR had physical conditions with mild or no disability and 17.2% had moderate educational problems. The prevalence of severe hearing loss as defined was 1.7 per 1000 aged 5-17. The prevalence of diabetes mellitus was 1.2 per 1000 children aged 0-17. Validation of the SCR for diabetes mellitus found 35/36 of the eligible children were correctly registered and no child was incorrectly included. The conflicting priorities for maintaining a register for the care of individual children, for service planning, and for epidemiological research are discussed.

Adolescent↗

Accuracy of diagnostic content of hospital activity analysis in infectious diseases.

Hospital activity analysis (HAA) was used to identify 2151 patients in whom the diagnostic code fell within the infectious disease categories of the International Classification of Disease. The clinical notes of these patients were examined by a doctor and in 414 (19.2%) the diagnostic coding was incorrect. Diarrhoea and viral infections were miscoded most often. The possibility of inaccuracy must be considered when HAA aggregated data are used for planning purposes. Accuracy could be improved if clinicians routinely completed the diagnostic sections of the HAA form.

Adult↗

Diabetes registers: a grassroots approach.

OBJECTIVES: To compile a register of diabetic patients within the catchment area of a district general hospital and evaluate the characteristics of the population using aggregated data from a general practice audit. DESIGN: Cross sectional study. Practices identified all known diabetics and completed a questionnaire from information in each patient's medical record. SETTING: Practices affiliated to a district audit group in south east England. MAIN OUTCOME MEASURES: Number of participating practices; prevalence of diabetes and its complications; and sex distribution of patients, age at diagnosis and review, treatment, and metabolic control. Comparisons with similar data from other studies. RESULTS: 41 out of 43 practices participated, and 2574 diabetic patients were identified (prevalence 1.18%). 52.4% of patients were male. The mean age was 61.6 years. 32% of patients were treated with insulin, 51.5% with oral hypoglycaemic agents, 16.5% with diet alone. The mean random blood glucose concentration was 10.4 mmol/l and glycosylated haemoglobin 10.1%. 8% had proteinuria, 7% a history of myocardial infarction, 5% a history of stroke, and 2% a diabetes related amputation. These proportions were not significantly different from those found in studies performed by different methods in Poole, Islington, Powys, Trowbridge, and Southall. CONCLUSION: It is feasible to compile a register of diabetic patients in a district and evaluate their characteristics by using only general practice sources.

Age of Onset↗

Use of vitamin B-12 in Leicestershire practices: a single topic audit led by a medical audit advisory group.

OBJECTIVE: To encourage active participation of Leicestershire general practitioners and their staff in audit; to examine the use of vitamin B-12 injections and to achieve a more appropriate use. DESIGN: Implementation of an agreed audit protocol, with central analysis and feedback of anonymous and aggregated data by a medical audit advisory group. SETTING: All 147 Leicestershire practices. MAIN OUTCOME MEASURES: Participation in the complete audit cycle, comparison of actual use of vitamin B-12 injections with agreed criteria of use, and assessment of improvement in use. RESULTS: 264 general practitioners (55% of all doctors from 49% of all Leicestershire practices) completed both phases of the audit cycle, and 321 (67%) completed phase 1 only. Twenty four (16%) practices failed to participate from the outset, and a further 58 (35%) dropped out at various stages. Only 10 of the 37 singlehanded practices completed the audit, although 34 initially agreed to participate. If singlehanded practices were excluded, 56% (61) of practices completed both phases of the audit cycle. In total 1714 patients received B-12 injections. Appropriate use increased from 62% in phase 1 to 72% in phase 2 of the audit; there was a 32% reduction in the number of patients inappropriately receiving B-12 (521 to 352), and the proportion of patients receiving B-12 at the correct frequency rose from 58% to 72%. The proportion of patients in whom all the diagnostic criteria for pernicious anaemia were established before treatment with B-12 was 27% in phase 1 and 28% in phase 2. CONCLUSION: Our study suggests that single topic audits organised by a medical audit advisory group can encourage large numbers of general practitioners to participate and can bring about changes in behaviour resulting in improvements in standards of care. Nevertheless, advisory groups will need to devise strategies to encourage even higher levels of involvement, most particularly from singlehanded practices.

Anemia, Pernicious↗

Secular trends in heart rate in young adults, 1949 to 2004: analyses of cross sectional studies.

OBJECTIVE: To investigate secular trends in resting heart rate in young adults. DESIGN, SETTING, AND PARTICIPANTS: A series of cross sectional cohorts of first year undergraduates (5562) aged 16-24 years who attended Queen's University Belfast from 1949 to 2004 and underwent health checks at the university health centre. MAIN OUTCOME MEASURE: Resting heart rate. RESULTS: Crude aggregate data for 1949-59 showed a secular decline in heart rate in male and female students. Both unadjusted and fully adjusted analyses of data from 1975 onwards-controlled for age, body mass index, height, and smoking-showed a U shaped association between heart rate and year of entry to university in both sexes (p < 0.001): heart rate initially declined and then increased from the mid 1980s. Trends were similar in smokers and non-smokers and in students with normal body size and those who were overweight. However, heart rate in the 693 (28%) male students reporting the most physical activity remained stable and lower than that of men who participated in less physical activity over the period of the study. Similarly in female students, heart rate was generally lower in the 210 (10%) who participated in the most physical activity than in those who engaged in less physical activity. CONCLUSIONS: The decline in heart rate in young adults occurring at least 50 years ago and continuing until the 1980s is consistent with other favourable findings on cardiovascular health in this age group and with observed long term declines in cardiovascular mortality. The more recent rise in heart rate, not accounted for by increases in overweight, prompts concern that recent favourable trends in cardiovascular disease risk may not be maintained. Among students who took part in the highest levels of physical activity the finding of low and stable heart rates points to the importance of exercise in maintaining cardiovascular health. Measurement of heart rate in population surveys would provide a simple tool to assist monitoring cardiovascular health.

Adolescent↗

Microtome: histopathology day book based on a microcomputer system.

The development of a histopathology day book based on a microcomputer is described. The system has the capacity to search on file records for details of previous specimens from current patients. It also possesses a SNOP/SNOMED input and search system that can aggregate data for analytical and other purposes. The system is relatively inexpensive and is user friendly. It has been developed within and is exclusively devoted to the requirements of a histopathology department and is designed to permit expansion to a multiuser system.

Computers↗

Daily mortality and "winter type" air pollution in Athens, Greece--a time series analysis within the APHEA project.

STUDY OBJECTIVE: There is evidence that air pollution in Athens between 1975 and 1987 had adverse short term health effects. The short term effects of "winter type" air pollution on the daily total number of deaths are investigated for the period 1987-91 as part of the European Community multi centre APHEA project. DESIGN: A temporal study using aggregated data is presented. The associations of the daily time series of three pollutants, sulphur dioxide (SO2), black smoke (BS), and carbon monoxide (CO) and the daily total number of deaths in the Athens area were assessed. DATA AND METHODS: The average measurement from three stations was used for each pollutant. The daily number of deaths was recorded from the Athens Town Registry and the registries of the 18 municipalities contiguous to Athens. Data on the mean daily temperature (degree C) and relative humidity (%) were also used. Poisson autoregressive models that also allowed for overdispersion were used. Seasonality, other long term patterns, temperature, humidity, day of the week, and holidays were adjusted for. Several a priori defined pollutant transformations and lags were investigated. One day measurements as well as cumulative exposure effects were assessed. Effect modification by season as well as among pollutants was tested. MAIN RESULTS: Linear terms were used for all pollutants. The magnitude of the effect was greater at lags 0 for CO and 1 for BS and SO2 gradually declining after lag 1. For an increase of 100 micrograms/m3 in SO2 and BS there were corresponding increases (95% CI) of 12% (7%, 16%) and 5% (3%, 8%) in the daily total numbers of deaths, while for an increase of 10 micrograms/m3 in CO the increase (95% CI) in the daily total number of deaths was 10% (5%, 15%). A significant interaction of the effects of SO2 with season were found. The strongest effect was observed during the winter, when higher levels of SO2 were observed. A stronger effect of SO2 on the daily total number of deaths was observed when the levels of BS were > 100 micrograms/m3. CONCLUSIONS: These results strengthen the evidence of a causal association between ambient particle, SO2, or CO levels in the air and the daily total number of deaths and points to an important public health issue for the Athens population.

Air Pollution↗