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The eradication of Encephalitozoon cuniculi from a specific pathogen-free rabbit colony.

Encephalitozoan cuniculi was discovered in a large specific pathogen-free rabbit colony during routine quality assurance testing. By using a modified India-ink immunoreaction test we were able to test the entire colony for antibodies to Encephalitozoon cuniculi. The prevalence of the disease was approximately 5%. All seropositive animals were culled, and another test of the entire colony, carried out 4 weeks later, revealed one seropositive rabbit which was also culled. Two subsequent screenings of the whole colony have shown no further seropositive animals.

Animals↗

The pathology of cytauxzoonosis in a tsessebe (Damaliscus lunatus).

A natural, fatal case of cytauxzoonosis in a tsessebe is reported. The macroscopic and histologic pathology are described, the most significant lesion of which was severe, focal disseminate necrosis and haemorrhage in the liver. Similar but less severe lesions were present in the heart, skeletal muscles, kidneys and spleen in conjunction with a moderate interstitial pneumonia. Schizonts were found within mononuclear phagocytes and multi-nucleated syncytial cells in the liver, lungs, spleen and kidneys. Parasitised erythrocytes with up to 4 piroplasms per cell were seen. The ultrastructural features of the parasite are consistent with those of Cytauxzoon spp.

Animals↗

A host-parasite catalogue of the haematozoa of the sub-Saharan birds.

The prevalence of avian haematozoa in 826 species of birds representing 73 families of sub-Saharan birds as recorded in the literature or in the files of the International Reference Centre for Avian Haematozoa and the Veterinary Research Institute is presented. The most commonly occurring blood parasites were members of the genus Haemoproteus which were represented by 63 species which occurred in 19.1% of the sample. Twenty-five species of Leucocytozoon were recorded in 8.3% of the birds and represented the second most frequently encountered group of haematozoa. Species of Plasmodium, Trypanosoma, and filarioids (as microfilariae) occurred in 3.5%, 2.5% and 2.8% respectively of the birds sampled; species of Aegyptianella, Atoxoplasma, Babesia and Hepatozoon were infrequently seen. An annotated list of the birds examined for haematozoa is presented and brief descriptions of the species of Haemoproteus and Leucocytozoon in birds of the sub-Saharan zone are included.

Africa↗

Settling rates and density of spores of Henneguya doori (Myxosporea) in water.

Using Henneguya doori from Perca flavescens (yellow perch), the current study provides a method for examining settling rates, attitudes, and densities of myxosporean spores. Fresh and fixed spores settled similarly at 8.34 x 10(-6) m/sec and 8.53 x 10(-6) m/sec, respectively. The spores settled in the anterior end first, did not rotate, and had a specific gravity of 1.062. Comparison of settling rates proved more effective for determining spore density than direct chemical estimation. The slow rate of settling permits effective dispersal in open water.

Animals↗

Neospora caninum (Apicomplexa) in a stillborn goat.

Tissue cysts of Neospora caninum were found in sections of brain from a stillborn pygmy goat. The tissue cysts had 1-2-microns-thick cyst walls and stained with anti-N. caninum serum in an immunohistochemical test. Glial nodules, mononuclear perivascular cuffing, and foci of inflammation were associated with N. caninum tissue cysts throughout the brain. This report indicates that N. caninum can be transmitted transplacentally in goats.

Animals↗

In vitro oxygen consumption and motility of Cryptobia salmositica, Cryptobia bullocki, and Cryptobia catostomi (Sarcomastigophora: Kinetoplastida).

Cryptobia salmositica (pathogenic and vaccine strains), Cryptobia bullocki (pathogenic), and Cryptobia catostomi (nonpathogenic) have similar oxygen consumption rates (0.17 +/- 0.01 nm O2/10(6) parasites). Incubation with sodium azide (5 microliters of a 1-M solution to 1 ml of parasite suspension, i.e., a 5-mM final concentration) reduced the oxygen consumption by approximately 4.5-fold. Motility of the parasites was also greatly reduced in sodium azide. The oxygen consumption and motility of the parasites returned to preazide treatment levels when the azide was removed even after 24 hr of incubation in sodium azide. The activities of hexokinase, pyruvate kinase, and cytochrome C oxidase were not detected in the 3 species of Cryptobia.

Animals↗