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Choice of oviposition site between surface of the medium and paper in four Indian species of Drosophila.

Choice of oviposition site between surface of the medium and paper positioned vertically on the medium by females was studied in four species of Drosophila: D. ananassae, D. bipectinata, D. malerkotliana, and D. biarmipes. Several geographic strains of these species were used. Females of all the four species laid greater proportions of eggs on the surface of medium as compared to surface of the paper. Thus all the four species tested show preference for oviposition on surface of medium. When the comparison is made for oviposition on paper among the four species, D. ananassae lays more eggs on the surface of paper as compared to other three species.

Animals↗

Filter paper collection of plasma for IGF-I test in patients with acromegaly.

The concentration of insulin-like growth factor I (IGF-I) was determined in paired specimens of liquid plasma and of plasma that had been applied to a filter paper disk, dried, and eluted; the measurements were performed by an immunochemiluminometric assay (ICMA). The 129 paired assays showed good correlation: y = 1.04x + 4.99; R2 = 0.934, where y is the result on dried samples and x is that on liquid samples. In 18 patients with acromegaly who lived on an island off the West Pacific Rim, plasma specimens were collected, applied to filter paper, dried, and mailed to a laboratory in the Upper Midwest of the United States. IGF-I was eluted from the filter paper disks and measured by ICMA. IGF-I concentrations ranged from 101 to 254 ng/ml in 11 patients with newly diagnosed but untreated acromegaly, 138 to 503 ng/ml in 4 patients with recurrent acromegaly, and 27 to 88 ng/ml in 3 patients with surgically cured acromegaly. These data demonstrate that plasma specimens of patients with acromegaly can be collected on filter paper disks and mailed to a laboratory on a distant continent for measurement of IGF-I. Such use of laboratory resources in one continent to help another continent, "Laboratories Without Borders," is somewhat analogous to the program "Doctors Without Borders."

Acromegaly↗

Controlling calcium precipitation in an integrated anaerobic-aerobic treatment system of a "zero-discharge" paper mill.

The pulp and paper industry uses significant amounts of water and energy for the paper production process. Closing the water cycles in this industry, therefore, promises large benefits for the environment and has the potential of huge cost savings for the industry. Closing the water cycle on the other hand also introduces problems with process water quality, quality of the end-product and scaling, owing to increased water contamination. An inline treatment system is discussed in which anaerobic-aerobic bioreactors perform a central role for removing both organic and inorganic pollutants from the process water cycle. In the proposed set-up, the organic compounds are converted to methane gas and reused for energy supply, while sulphur compounds are stripped from the process cycle and calcium carbonate is removed by precipitation. Improved control of the treatment system will direct the inorganic precipitates to a location where it does not adversely affect paper production and process water treatment. A simulation program for triggering and controlling CaCO3 precipitation was developed that takes both biological conversions and all relevant chemical equilibria in the system into account. Simulation results are in good agreement with data gathered in a full-scale "zero-emission" paper plant and indicate that control of CaCO3 precipitation can be improved, e.g. in the aerobic post-treatment. Alternatively, a separate precipitation unit could be considered.

Bacteria, Aerobic↗

Automated time-resolved immunofluorometric assay for Toxoplasma gondii-specific IgM and IgA antibodies: study of more than 130,000 filter-paper blood-spot samples from newborns.

BACKGROUND: To screen for congenital toxoplasmosis, we developed a time-resolved immunofluorometric assay for the simultaneous detection of Toxoplasma gondii-specific IgM and IgA in filter-paper samples collected from newborns 4-7 days after birth. METHODS: The assay was performed on the AutoDELFIA, and results were calculated based on the ToxoM WHO Third International Reference Serum. Comparison with an in-house mu-capture immunoassay was carried out retrospectively on filter-paper samples from children with confirmed congenital toxoplasmosis. Prospectively the assay was compared with a mu-capture immunoassay on 68 394 samples and a commercially available assay on another 69,467 samples. Before serum was requested from the newborn, positive samples were tested for IgA and IgM separately and in an IgM-immunosorbent agglutination assay developed for filter-paper samples. RESULTS: Intra- and interassay variations (CVs) were 8% and 16%, respectively. The cutoff of 5 units/mL produced a 0.5% retest rate. The assay detected 13 of 18 (72%) samples from newborns diagnosed with congenital toxoplasmosis in the retrospective study. Prospectively, the assay identified 24 newborns who were later diagnosed with congenital toxoplasmosis. Results for all 24 cases were positive by the respective comparison method. No cases were detected solely by the IgA antibodies in the sample. CONCLUSION: Neonatal screening for congenital toxoplasmosis can be automated by use of purified europium-labeled antigen for detection of T. gondii-specific IgM and IgA eluted from filter-paper samples.

Animals↗

Bioremediation of paper and pulp mill effluents.

Pulp and paper mill effluents pollute water, air and soil, causing a major threat to the environment. Several methods have been attempted by various researchers throughout the world for the removal of colour from pulp and paper mill effluents. The biological colour removal process uses several classes of microorganisms--bacteria, algae and fungi--to degrade the polymeric lignin derived chromophoric material. White rot fungi such as Phanerochaete chrysosporium, Corius versicolor, Trametes versicolor etc., are efficient in decolourizing paper and pulp mill effluents. Gliocladium virens, a saprophytic soil fungus decolourised paper and pulp mill effluents by 42% due to the production of hemicellulase, lignin peroxidase, manganese peroxidase and laccase.

Basidiomycota↗

Nutrient minimisation in the pulp and paper industry: an overview.

This paper reviews nutrient issues within the pulp and paper industry summarising: nitrogen and phosphorus cycles within treatment systems; sources of nutrients within pulping and papermaking processes; minimising nutrient discharge; new approaches to nutrient minimisation; and the impact of nutrients in the environment. Pulp and paper industry wastewaters generally contain insufficient nitrogen and phosphorus to satisfy bacterial growth requirements. Nutrient limitation has been linked to operational problems such as sludge bulking and poor solids separation. Nutrients have been added in conventional wastewater treatment processes to ensure optimum treatment performance. Minimising the discharge of total nitrogen and phosphorus from a nutrient limited wastewater requires both optimised nutrient supplementation and effective removal of suspended solids from the treated wastewater. In an efficiently operated wastewater treatment system, the majority of the discharged nutrients are contained within the biomass. Effective solids separation then becomes the controlling step, and optimisation of secondary clarification is crucial. Conventional practice is being challenged by the regulatory requirement to reduce nitrogen and phosphorus discharge. Two recent developments in pulp and paper wastewater treatment technologies can produce discharges low in nitrogen and phosphorus whilst operating under conventionally nutrient limited conditions: i) the nutrient limited BAS process (Biofilm-Activated Sludge) which combines biofilm and activated sludge technologies under nutrient limited conditions and ii) an activated sludge process based on the use of nitrogen-fixing bacteria. Aerated stabilisation basins often operate without nutrient addition, relying on settled biomass in the benthal zone feeding back soluble nutrients, or the fixation of atmospheric nitrogen. Thus effective nutrient minimisation strategies require a more detailed understanding of nutrient cycling and utilisation. Where it is not possible to meet discharge constraints with biological treatment alone, a tertiary treatment step may be required. In setting nutrient control guidelines, consideration should be given to the nutrient limitations of the receiving environment, including other cumulative nutrient impacts on that environment. Whether an ecosystem is N or P limited should be integrated with wastewater treatment considerations in the further design and development of treatment technology and regulatory guidelines. End-of-pipe legislation alone cannot predict environmental effects related to nutrients and must be supplemented by an effects-based approach.

Biofilms↗

[Utilization of organic resources in paper pulp waste liquid].

In this paper, one hundred percent of condensed sulfate paper pulp waste liquid was used as the raw material of adhesive, and the activation of its lignin as well as the improving effects of phenol formaldehyde resin and polyfunctional aqueous polymer isocyanate (PAPI) were studied. The results showed that adding formaldehyde to the waste liquid could increase the reactivity of contained lignin, and adding 30% phenol formaldehyde resin or 20% PAPI could make the waste liquid in place of pure phenol formaldehyde resin for producing class I plywood. Furthermore, the cost could be reduced by 55.5% and 49.0%, respectively, in comparing with pure phenol formaldehyde resin. This approach fully used the organic resources in paper pulp waste liquid, reduced environment pollution at the same time, and had unexceptionable economic, social and ecological benefits. The feasibility of preparing adhesives from paper pulp waste liquid was also analyzed by infrared spectrum.

Formaldehyde↗

[Study on the process of degrading filter paper cellulose by Sporocytophaga sp. JL-01].

Sporocytophaga is a kind of bacteria that can glide on the solid medium surface. It can decompose the cotton and filter paper after 6 days cultured. The sporocytophaga can only produce a low extracellular carboxymethylcellulase activity and no other cellulase activities. In this work, the process about degradation of filter paper fibre by a strain of Sporocytophaga was studied using scan electronmicroscopy. The morphological changes that Sporocytophaga sp. JI-01 grew on filter paper were observed. The study revealed the JL-01 adhered tightly to the surface of filter paper fibre or penetrated into interior of the fibre through its 2.5 microm - 4.0 microm bacilliform cells in the process of degrading. The bacilliform cells degrade the cellulose strongly and produced a mass of sticky polysaccharides. At the anaphase of cultured, the bacteria existed as a cycloidal dormancy body-sporocyst.

Bacterial Proteins↗

Comparison of behaviour of very low density lipoproteins of type III hyperlipoproteinaemia on electrophoresis on paper and on agarose gel with a note on a late (slow) pre-beta VLDL liproprotein.

The diagnosis of type III hyperlipoproteinaemia is based on the presence of a very low density lipoprotein (VLDL) that on electrophoresis has beta instead of the usual pre-beta mobility. This definition is based on paper electrophoresis. Lipoproteins of type III sera were studied by simultaneous electrophoresis on paper and on agarose gel. On paper the ultracentrifugally isolated VLDL had beta mobility, but on agarose gel their mobility was slightly more rapid than beta. It is thus important to consider the electrophoretic conditions in the diagnosis of type III. The diagnosis of type III is further complicated by the presence of agarose gel electrophoresis - contrary to paper - of a second, a 'late pre-beta VLDL' lipoprotein (LPbeta) in 20-30 per cent of both normal and hyperlipoproteinaemic sera. Quantitative lipoprotein analysis of 609 consecutive sera showed that when LPbeta was present, both in normo- and hyperlipoproteinaemia, certain significant lipoprotein changes occurred. Thus VLDL had a high cholesterol content and a raised cholesterol to triglyceride ratio. Furthermore, the triglyceride content of low density lipoproteins (LDL) was increased. These lipoprotein abnormalities are also seen in type III hyperlipoproteinaemia. With regard both to chemical composition of VLDL and LDL and to electrophoretic mobility on agarose gel of VLDL, type III hyperlipoproteinaemia and LPbeta are similar. The possibility of a metabolic relation is discussed.

Cholesterol↗

Paper tape in the closure of abdominal wounds.

Wounds closed by adhesive tapes have less inflammatory reaction, a lower rate of wound infection, greater tensile strength and better cosmetic results than sutured or stapled wounds. Special backing tapes, such as Steri-Strip, have been reported to have satisfactory results in closing laparotomy wounds. However, they are more expensive than paper tape. Sterile paper tape had adequate adhesive and supporting strength in the closure of abdominal wounds. The wounds closed with paper tape healed well and had satisfactory cosmetic results. Paper tape is a nonexpensive and convenient alternative to close abdominal wounds.

Abdomen↗

Activated paper as an immunoabsorbent.

Some workers when using cyanogen bromide activated paper as a solid immunoabsorbent have reported variable success in radio immuno assays. A quantitative investigation has been made of some of the factors which effect the activation of various papers with CNBr, and also of the subsequent coupling reaction with the sensitizing antigen. Different papers have been found to react in different ways, and some papers take up so little antigen that they are unsuitable for use for this purpose.

Absorption↗

Fluorescence polarization immunoassay of gentamicin or netilmicin in blood spotted on filter paper.

In this improved simple method for determination of aminoglycoside antibiotics in dried-blood spots on filter paper, gentamicin or netilmicin is recovered from the blood spot most effectively by incubation for 60 min in an ultrafiltration tube containing 500 microL of 0.5 mol/L Na2HPO4 buffer. The eluates from the paper are centrifuged, then transferred to an Abbott TDx cartridge for measurement of gentamicin or netilmicin by fluorescence polarization immunoassay. The dried sample on paper is stable for about eight days at ambient temperature. Intra-assay CVs for gentamicin and netilmicin are less than 8.5% and less than 6.1%, respectively. Analytical recovery of gentamicin and netilmicin from the paper exceeded 90%. This method permits simple blood collection and monitoring of the therapeutic concentration of gentamicin or netilmicin in serum, particularly that of newborn infants and small children.

Blood Stains↗

Filter paper blood sampling for glycated haemoglobin determination and its use in the control of diabetes mellitus.

Blood sampling on filter paper was tested for determination of glycated haemoglobin. The method showed coefficients of variation of 3.4% and 4.1%, and linearity coefficients of 0.978 and 0.91 for the microchromatographic and colorimetric methods respectively. A blood sample on filter paper impregnated with 5% ethylene glycol solution remains stable for 8 days at room temperature. In a group of 30 diabetics educated in the filter paper blood sampling technique, no statistically significant differences were registered among the mean values of their blood glucose profiles, glycated haemoglobin levels determined at the hospital and those obtained at home 14 days after discharge. In conclusion, the use of blood spotted on filter paper seems a cheap and convenient method for collecting, storing and transporting samples for analysis of glycated haemoglobins. It is also a useful alternative for home monitoring of diabetics. Moreover, it could also be useful in epidemiological studies of diabetes.

Adult↗

A mortality study of Finnish pulp and paper workers.

Mortality among workers in the Finnish pulp and paper industry was evaluated in a retrospective cohort study of 3520 workers who had been employed continuously for at least one year between 1945 and 1961. Six subcohorts were formed according to six work areas: sulphite, sulphate, paper, and board mills, maintenance department, and power plant. National mortality rates were used for comparison. The mortality of a cohort of 1290 sawmill workers was also studied in order to have a socially, geographically, and occupationally similar group without the exposures typical of the pulp and paper industry for comparison. Smoking habits were surveyed. Mortality was followed up until 31 December 1981. Overall mortality for the entire cohort did not differ from that expected (1044 obs, 1029.4 exp, SMR 101), but there was an excess of deaths from diseases of the circulatory system among the men (489 obs, 404.9 exp, SMR 121). This was due to the excess of deaths from ischaemic heart disease found among the men at the sulphite, sulphate, and paper mills, maintenance department, and power plant, but not at the sawmill. The existing smoking data did not explain this finding and it may therefore be associated with occupational exposures.

Adolescent↗

Use of Whatman chromatography paper for serological studies in leprosy in the field.

Recently W.H.O. recommended further serological studies on leprosy using samples collected on filter paper strips. A study was therefore taken up at C.L.T. and R.I., Chengalpattu, to find out the relative efficiency of the serological tests conducted on sera and filter paper eluates. FLA-ABS and FA-BCG tests were carried out on 100 paired samples. With the former, exactly the same titres were obtained in 42% and with the latter in 52% cases. Most of the others showed positivity at one-dilution-low titres. In 6-8% cases only, the filter paper technique showed negativity. The correlation co-efficient was +0.87 with FLA-ABS and +0.85 with FA-BCG test. The filter paper technique was operationally more convenient and was acceptable without substantially loosing the efficacy.

Antibodies, Bacterial↗

Measurement of glycosylated whole-blood protein for assessing glucose control in diabetes: collection and storage of capillary blood on filter paper.

We present data on the use of filter-paper blood collection for measurement of glycosylated whole-blood proteins (gWB) (hemoglobin and plasma proteins). A capillary blood sample, obtained by fingerprick, is spotted directly onto filter paper (Schleicher & Schuell 903). The blood spot is washed briefly with alcohol (ethanol or isopropanol) to remove free glucose and dried before shipment to the laboratory. In the laboratory, the blood is eluted from the paper and analyzed for gWB by a colorimetric method. The gWB is primarily a measure of glycosylated hemoglobin (gHb) with a small contribution from glycosylated plasma protein. Concentrations of gWB and gHb are highly correlated (r = 0.91). The filter-paper method offers advantages over currently available methods for quantifying gHb and may be particularly useful in screening for diabetes and for assessing glycemic control in patients from remote areas.

Blood Proteins↗

Contact urticaria and airway obstruction from carbonless copy paper.

A 27-year-old woman experienced pruritus, eye and throat irritation, hoarseness, shortness of breath, and fatigue within half and hour of exposure to carbonless copy paper. On two separate occasions, she was purposely challenged in a controlled-blinded fashion with portions of the carbonless copy paper. This resulted on both occasions in contact urticaria of the hand that held the paper and changes in pulmonary function flow-volume loops characteristic of upper airway obstruction. To determine if alterations in prostaglandin (PG) metabolism might explain these findings, plasma PGF2 alpha and thromboxane B2 (both capable of causing these symptoms) were measured before and during the second exposure period. Both PGF2 alpha and thromboxane B2 increased substantially. We conclude that the cutaneous and respiratory symptoms induced by carbonless copy paper were probably related to PG release.

Adult↗

Complaints relating to the handling of carbonless copy paper in Sweden.

No specific relationship could be established between complaints and chemicals used in the brands of carbonless copy paper on the Swedish market. The symptoms were non-specific and very variable. Any office encountering these problems must have them analyzed separately to find the local explanation. Carbonless copy paper has been in widespread use for more than 20 years. Although several reports have been published about suspected health hazards from such papers, none has been able to prove any relationship between carbonless copy papers in general and health hazards (see bibliography). That all chemicals in a product are absolutely safe can never be proved, but if all efforts to prove them dangerous fail, the suspicions of their hazards must diminish. Investigations must concentrate on finding the local cause of the trouble.

Carbon↗