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PubMed1991

Staphylococcus aureus nasal carriage in patients with rhinosinusitis.

Toxic shock syndrome has been associated with rhinologic surgery and medical devices, and it has been linked to a circulating exotoxin of a toxogenic strain of Staphylococcus aureus. One hundred forty patients with rhinosinusitis were studied. Nasal cultures were obtained. The microbiological characteristics are described. The carrier rate for Staphylococcus aureus was 35%. Thirty percent of patients selected for surgery were Staphylococcus aureus carriers. Toxin-capable isolates were identified in 40% of those tested. Users of cocaine, topical decongestants, and steroid sprays had a statistically higher rate of Staphylococcus aureus carriage compared to nonusers. It is hoped that by identifying the population at risk and defining the factors associated with the development of toxic shock syndrome, a cogent policy of prevention can be established.

Adolescent↗

PubMed2005

In vitro activity of 24 antimicrobial agents against Staphylococcus and Streptococcus isolated from diseased animals in Japan.

A total of 88 Staphylococcus and 61 Streptococcus isolates from diseased animals throughout Japan were examined in 2000 for the minimum inhibitory concentrations of 24 different antimicrobials by the agar dilution method standardized by the Japanese Society of Chemotherapy. The resistance rates to aminobenzylpenicillin (36.4%) and benzylpenicillin (35.2%) were high in Staphylococcus isolates, and those to oxytetracycline (45.9%) and kanamycin (21.3%) were high in Streptococcus isolates. Two isolates resistant to oxacillin harbored the mecA gene. One was Staphylococcus epidermidis derived from a pig with arthritis, and the other Staphylococcus cohnii from a head of cattle with mastitis.

Animals↗

PubMed1989

[Simultaneous occurrence of enterotoxigenic Staphylococcus aureus on the hands, mouth and feces of asymptomatic carriers].

One hundred and twelve asymptomatic individuals were analysed with regard to the simultaneous incidence of Staphylococcus aureus on their hands and in their nose, mouth and stools, in the city of S. Paulo (Brazil). A total of 40 (35.7%) individuals were detected as carriers of this microorganism. Among these carriers, 27 (67.5%) were positive in only one of the four niches studied, 8 (20.0%) in two and 5 (12.5%) in three niches. They were identified 113 Staphylococcus aureus strains, and 28 (24.8%) of these strains, isolated from 9 (22.5%) carriers, produced enterotoxin. Of these strains, 7 (25.0%) produced type A enterotoxin, 6 (21.4%) of type B, 11 (39.3%) type C and 4 (14.3%) produced both type A and C. The phage typing of the 113 Staphylococcus aureus strains revealed the predominance of the strains lysed by phages belonging to the Group I/III/NC (16.8%). The results obtained did not demonstrate the simultaneous incidence of Staphylococcus aureus strains in samples collected from mouth, hands and stools of the group studied.

Carrier State↗

PubMed2005

Study of viridans streptococci and Staphylococcus species in cleft lip and palate patients before and after surgery.

OBJECTIVE: To determine the effect of surgery on types and colony count of Streptococcus and Staphylococcus species in cleft lip and palate (CLP) patients. DESIGN: Saliva samples were collected after the morning meal by placing a sterile cotton swab in the vestibule of the oral cavity from cleft lip and palate patients immediately preoperative and 12 weeks postoperative. Normal children were examined as a control group. Samples were cultured; Staphylococcus and Streptococcus isolates were identified and quantified. PATIENTS: Fifteen cleft lip and palate patients and 22 normal children, aged 3 to 39 months were examined. RESULTS: Streptococcus mitis biovar 1, Streptococcus salivarius and Streptococcus oralis of the viridans group of streptococci were the most commonly found in normal children, as well as in cleft lip and palate children. In the cleft lip and palate group, mean streptococcal count was 32.41 (29.80) and 46.46 (42.80) in the pre- and postoperative periods, respectively; in the normal group, the count was 20.93 (27.93) and 49.92 (34.72) at 0 week and 12 weeks, respectively. Staphylococcus aureus was the most common Staphylococcus species found in CLP patients, representing 47.4% postoperatively. In the cleft lip and palate children, mean staphylococcal count was 5.34 (8.13) and 0.56 (0.92) in the pre- and postoperative periods, respectively; in normal children, the count was 0.82 (1.98) and 0.60 (2.55) at 0 and 12 weeks, respectively. The differences were statistically significant only for the staphylococcal count between pre- and postoperative periods in children with cleft lip and palate as tested by analysis of variance (p < .05). CONCLUSIONS: Cleft lip and palate patients had more colonization by S. aureus compared with normal children, and the colony count decreased significantly following surgical repair of the cleft lip and palate.

Analysis of Variance↗

PubMed1994

Rhabdomyolysis associated with bacteremic pneumonia due to Staphylococcus aureus.

A case of rhabdomyolysis associated with bacteremic pneumonia due to Staphylococcus aureus is reported. A 58-year-old man was admitted because of severe left lobar pneumonia, and presented myoglobinuria on admission. The serum creatine phosphokinase level was transiently elevated and myoglobinuria were disclosed. Sputum and venous blood cultures grew Staphylococcus aureus. Rhabdomyolysis has rarely been reported in cases of bacterial infection, especially those due to Staphylococcus aureus. This case might be an extremely rare case of rhabdomyolysis complicating Staphylococcus aureus bacteremia following severe left upper lobar pneumonia.

Bacteremia↗

PubMed1980

Virulence of three strains of Staphylococcus aureus isolated from clinically infected bovine mammary glands.

Factors in the ability of three strains of Staphylococcus aureus to cause bovine mastitis were studied. Each strain was evaluated fro growth rate, clump size, and resistance to phagocytosis and intracellular kill by polymorphonuclear leukocytes isolated from milk of nine cows. Variation among cows in the ability of polymorphonuclear leukocytes to phagocytose and of skim milk to support phagocytosis was assessed. Virulent strains of Staphylococcus aureus had a smaller clump size (1.5 colony forming units [CFU]/clump) than the less virulent strain (2.6 CFU/clump) after 180 min of growth in skim milk samples. When the skim milk samples were sonicated to disperse the staphylococci, population density of strains of Staphylococcus aureus did not differ. Phagocytosis and total number of staphylococci killed by polymorphonuclear leukocytes were depressed for virulent strains. Ability of polymorphonuclear leukocytes isolated from individual cows to phagocytose each strain of Staphylococcus aureus varied. The correlation with treated cases of clinical mastitis per lactation was -.54. The ability of skim milk to support phagocytosis by polymorphonuclear leukocytes also differed among cows. The correlation with treated cases of clinical mastitis per lactation was -.51.

Animals↗

PubMed1991

Isolation of Staphylococcus aureus from sites other than the lactating mammary gland.

The body sites of 163 heifers, bedding and feedstuff samples, flies, and hands and nares of the research personnel were sampled in order to determine the sources of Staphylococcus aureus in a dairy herd other than the lactating mammary gland. Lesions on the udder of lactating animals and the air in the milking parlor were also sampled. Staphylococci isolated from bedding samples were identified as to species. Staphylococcus aureus was isolated from all sources examined except flies. An enrichment procedure was necessary for isolating S. aureus from two bedding samples although other Staphylococcus species were present in high numbers. The designation "environmental staphylococci" is proposed for Staphylococcus species that were apparently free-living in the environment.

Air Microbiology↗

PubMed1998

Sources of intramammary infections from Staphylococcus aureus in dairy heifers at first parturition.

The study objective was to identify probable sources and modes of transmission of 91 Staphylococcus aureus isolates obtained from the colostrum of 76 heifers at parturition. Sources cultured were milk (including colostrum), heifer body sites (teats, muzzle, rectum, vagina, and lacteal secretions), and environmental sites (bedding, insects, housing, water, feedstuffs, humans, nonbovine animals, air, and equipment). Staphylococcus aureus isolates were characterized by 63 phenotypic traits. A similarity coefficient was calculated by herd to identify the S. aureus that most closely resembled the S. aureus obtained from heifer colostrum. Staphylococcus aureus from a heifer's colostrum was compared with all preexisting S. aureus isolates from that heifer's herd. Isolates that were > or = 90% similar were considered to be identical. Because 30 (of the 91) S. aureus isolates from heifer colostrum were collected prior to environmental sampling, only 61 S. aureus isolates from heifer colostrum were available for comparison among all three sources. Possible sources of S. aureus from heifer colostrum at parturition were milk (70%, 43 of 61 isolates), heifer body sites (39%, 24 of 61), environmental sites (28%, 17 of 61), or no identified source (16%, 10 of 61). Three heifers with intramammary infection (IMI) from S. aureus at parturition had the same S. aureus on their teats prior to parturition. Milk was the only source identified for 41% (25 of 61) of isolates from heifer colostrum. Isolates from heifer body sites were the only source identified for 5% (3 of 61) of heifer colostrum isolates. Staphylococcus aureus from the environment was never the sole possible source for S. aureus from heifer colostrum. Data suggest that the major sources of S. aureus IMI in heifers at parturition are milk and heifer body sites. Contact among heifers may be an important mode of transmission of S. aureus leading to IMI in heifers at parturition.

Animals↗

PubMed2000

Antimicrobial resistance patterns and plasmid profiles of Staphylococcus aureus isolated from milk and meat.

OBJECTIVES: To determine the frequency of resistance of Staphylococcus aureus to various antimicrobial agents, and the relationship between antimicrobial resistance of the isolates and carriage of plasmids. DESIGN: A random sampling of milk and meat samples was carried out. SETTING: Milk was collected from various dairy co-operative societies in Nairobi and Kiambu districts. Minced meat samples were purchased from various outlets in the city of Nairobi. SUBJECTS: Ninety six Staphylococcus aureus isolates from milk (seventy five isolates) and minced meat (twenty one isolates) samples. MAIN OUTCOME MEASURES: Plasmid profiles and antimicrobial susceptibility tests to ampicillin, lincomycin, penicillin, erythromycin, methicillin, minocycline, cotrimoxazole and chloramphenicol. RESULTS: Seventy one per cent of the isolates carried between one and six plasmids of molecular sizes ranging from 0.1 to 14.5 kilobases. High frequency of resistance was observed with lincomycin (67.7%), penicillin (66.7%) and cotrimoxazole (51%). A high percentage (76%) of isolates were susceptible to minocycline followed by erythromycin (57.3%). Most (80.2%) of isolates were multiply resistant to between two and six antibiotics. CONCLUSION: Most Staphylococcus aureus isolates were multiply resistant to various antimicrobial agents, but there was no apparent relationship between carriage of plasmids and antimicrobial resistance. Milk and meat may contain resistant Staphylococcus aureus posing a potential risk to consumers.

Animals↗

PubMed2004

PCR detection of Bacillus and Staphylococcus in various foods.

A broad-range PCR assay for the detection of bacteria belonging to Bacillus and Staphylococcus genera was developed. Primers targeting the bacterial 16S rRNA gene were newly designed and used in a PCR assay. To determine the specificity of the assay, 81 different bacterial strains (of 50 genera), 2 fungi, 3 animals, and 4 plants were tested. Results were positive for every tested Bacillus, Staphylococcus, or Aerococcus strain. In addition, the result for Listeria grayi was positive with lower PCR product. For all other bacterial strains and eukaryotes tested, results were negative. Bacterial DNA was prepared with the use of achromopeptidase and Chelex 100 resin from culture after growth in brain heart infusion medium. To test the sensitivity of this PCR assay for Bacillus or Staphylococcus genus, either Bacillus cereus or Staphylococcus aureus was inoculated into various foods with undetectable levels of endogenous microbial contamination as an indicator. Inoculation of bacteria at 10 to 30 CFU/g of food was followed by a 5-h enrichment culture step after which the PCR assay allowed the detection of bacterial cells. When the inoculation (B. cereus or S. aureus) of 10 to 90 CFU/g into noodle foods containing endogenous microflora (10(3) to 10(5) CFU/g) was followed by a 6-h enrichment culture step, the PCR assay detected the bacteria. Including the enrichment culture step, the entire PCR detection process can be completed within 8.5 h.

Bacillus↗

PubMedDate not supplied

[State of methicillin resistant Staphylococcus aureus in the Hospital of Navarre (2000-2002)].

BACKGROUND: The aim of the present paper was to determine the prevalence and characteristics shown by isolations of Methicillin Resistant Staphylococcus Aureus, isolated in our Microbiology Service. METHODS: Retrospective study, covering the years 2000, 2001 and 2002. Analysis was made of the origin of the infection (nosocomial or non-nosocomial), the unit of origin in the event of nosocomial origin, anatomical localisation of the sample, and pattern of antibiotic sensitivity. RESULTS: The isolations of Methicillin Resistant Staphylococcus aureus accounted for 7.88% of the Staphylococcus aureus isolated. Less than half of the strains (44.87%) had a nosocomial origin and were most often isolated in the exudates of wounds. With respect to the pattern of resistance, there was some 50% of resistance to erythromycin, some 43.60% to clindamycine and some 21.79% to mupirocin. CONCLUSIONS: The prevalence and resistance pattern in vitro of the isolations of Methicillin Resistant Staphylococcus Aureus in our hospital are lower than that published in other areas of Spain.

Anti-Bacterial Agents↗

PubMed1999

Nasal carriage of methicillin resistant Staphylococcus aureus in a cardiovascular tertiary care centre and its detection by Lipovitellin Salt Mannitol Agar.

Ecological niches of Staphylococcus aureus are the anterior nares. Carriage of Staphylococcus aureus in the nose appears to play a key role in the epidemiology and pathogenesis of infection. Numerous studier have shown that elimination of nasal carriage using Mupirocin also eliminated hand carriage and the spread of infections in hospitals. Lipovitellin-Salt-Mannitol Agar was used for screening, isolation and presumptive identification of Staphylococcus aureus from nasal carriers. From November; 97 to August'98, 724 nasal swabs were cultured and 18.23% of health care workers were found to be nasal carriers of Staphylococcus aureus. Of these 12.15% were carriers of MRSA. The carrier rate was highest in December' 97 (32.07%). All MRSA carriers were treated with local application of Mupirocin for three days. A study of the antibiogram of the clinical isolates during the corresponding period showed 100% susceptibility of MRSA to Vancomycin. Susceptibility of MRSA to Clindamycin, Netilmycin, Rifampicin & Ofloxacin was 86.6%, 69.5%, 66% & 64.7% respectively.

Bacteriological Techniques↗

PubMed2000

[Evaluation of methicillin-resistance in Staphylococcus aureus by the agar disk diffusion method and PCR].

Staphylococcus aureus is a widespread human pathogen. One the most striking characteritics of this bacterium is resistance to methicillin and all beta-lactam antibiotics. The agar disk diffusion method is the most widely used in vitro susceptibility test, but recently molecular methods, e.g. Polymerase Chain Reaction, have been also introduced. We compared the detection of methicillin resistant coagulase positive Staphylococcus aureus isolated from clinical materials in Silesian microbiological laboratories by diffusion method and PCR through the detection of nuc and mec A genes. Our results show that PCR used for the detection of mec A gene increases the detection of methicillin-resistant Staphylococcus aureus strains by 10% as compared to the agar disk diffusion method. Among Staphylococcus aureus strains, detected as methicillin-resistant, 17% of organisms showed no presence of mec A gene.

Colony Count, Microbial↗

PubMed2001

Phospholipase A(2) in rabbit tears: a host defense against Staphylococcus aureus.

PURPOSE: This study analyzed rabbit tears for anti-staphylococcal activity, the role of phospholipase A(2) (PLA2) in this reaction, and the ability of enzyme inhibitors to promote bacterial survival. METHODS: Contact lenses with Staphylococcus aureus were applied to scarified rabbit eyes. The colony-forming units (CFU) per cornea or lens were determined and pathology was scored by slit-lamp examination (SLE). The bactericidal activity was measured by incubating bacteria with rabbit tears or PLA2 at 33 degrees or 37 degrees C. Radiolabeled S. aureus was incubated with PLA2 or tears to quantify the release of a membrane component that was identified by thin-layer chromatography. Inhibitors of these reactions were also analyzed. RESULTS: Application of Staphylococcus, on contact lenses, to rabbit corneas resulted in bacterial killing and limited inflammation. Incubation of tears and bacteria (1:1; v/v) in tryptic soy broth at 33 degrees C decreased CFU approximately 4 logs. Tears (> or =30 microl) or PLA2 (> or =30 U) incubated with bacteria in phosphate-buffered saline were bactericidal. PLA2 (> or =0.2 U) or tears (> or =2 microl) cleaved bacterial membranes, liberating arachidonic acid. Spermidine or tetracaine inhibited cleavage of bacterial membranes by tears or PLA2 and spermidine promoted bacterial survival and growth in tears. Tears (60 microl) killed >99% of the bacterial inoculum, whereas bacteria incubated in tears plus spermidine approximately doubled in number. CONCLUSIONS: PLA2 in rabbit tears kills Staphylococcus by hydrolyzing bacterial membranes to release arachidonic acid. Spermidine and tetracaine inhibited PLA2 activity and spermidine protected Staphylococcus from PLA2 in rabbit tears.

Animals↗

PubMedDate not supplied

[Correlation between Staphylococcus carriage, specific antibody-production and AB0-blood grouping in plasma donors].

Interaction peculiarities of three components of the immune human homeostasis-antigens of blood groups AB0, staphylococcus antigens and antistaphylococcus antibodies have been investigated. Donors (85) of antistaphylococcus plasma immunized by staphylococcus anatoxin have been investigated. It is found that the nasal staphylococcus carriage in donors depends on the level of specific and natural antibodies and on the coincidence between the staphylococcus antigen structure and the protein substance of the specific blood group factors.

ABO Blood-Group System↗

PubMedDate not supplied

[Staphylococcus aureus sepsis in hospitalized non neutropenic patients: retrospective clinical and microbiological analysis].

Staphylococcus aureus is one of the leading agents of nosocomial infection among adult patients. The aim of this study was to determine the predisposing factors and secondary complications of Staphylococcus aureus septicemia (SAS) in non neutropenic patients, as well as the predictors of the outcome in non neutropenic patients with SAS. We performed a retrospective study of 56 cases of SAS that occurred from January 1997 through June 2001 in patients hospitalized in medical wards at the Policlinico Umberto I, "La Sapienza" University of Rome; we excluded surgical patients and those admitted to the intensive care unit. The median age was 61.9 years (range 24-89 years), 29 (51%) patients were male, and infection was hospital-acquired in 83.5% of cases. Metastatic infections were found in 12 patients (21.4%), with 6 (10.7%) developing infectious endocarditis; the relapse rate was 8.9%; 30.3% of Staphylococcus aureus isolates were methicillin-resistant. The overall mortality was 41% and the attributable mortality 28.5%. Twenty-nine patients who developed metastatic infections or died for sepsis were compared with 27 patients who did not develop complications. At univariate analysis, the following factors were associated with a complicated course: delay to adequate antibiotic therapy (2.46 vs 1.15 days, p < 0.03), persistent Staphylococcus aureus bacteremia during antibiotic therapy (3.56 vs 1.51 days, p = 0.01), septic shock (58.6 vs 3.7%, p < 0.002), bacteremic pneumonia as the source of bacteremia (17.2 vs 0%, p = 0.02), and the increased severity of illness at the onset of SAS as evaluated using an "illness score" (4.2 vs 2.1, p < 0.002). At multivariate analysis, septic shock (p < 0.01) and delay to adequate antibiotic therapy (p = 0.05) were confirmed as associated with a complicated outcome. SAS in non neutropenic patients is associated with significant morbidity consequent to a high rate of metastatic infectious disease and with a considerable related mortality.

Adult↗

PubMedDate not supplied

[Comparison of Staphylococcus spp. cellular and extracellular proteins by SDS-PAGE].

In this study, a total of fifteen staphylococcal strains belonging to different species were characterized by whole-cell and extracellular protein profiles using sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results are presented as dendrograms after quantitative analysis of the band patterns with a computer program. Visual inspection of protein bands and cluster analysis of protein patterns of 15 strains representing 10 Staphylococcus species showed that whole-cell and extracellular protein profiles differed in several protein bands in Staphylococcus aureus, S. epidermidis, S. simulans and other species of Staphylococcus; however, the differences were insufficient for reliable differentiation of Staphylococcus species by the SDS-PAGE method.

Bacterial Proteins↗

PubMed1992

[Nosocomial infections of methicillin-resistant Staphylococcus aureus and their detection].

In 142 strains of Staphylococcus aureus randomly isolated from clinical samples in a year, methicillin-resistant Staphylococcus aureus (MRSA) accounted for 79.6% (113/142). MRSA in the samples from the respiratory, burning and hematological departments came to 92.0% (23/25), 84.6% (11/13) and 83.3% (30/36) in proportion respectively. 79.6% of MRSA strains were isolated from the patients in whom the infections were confirmed to be hospital-acquired. All the strains of Staphylococcus aureus examined were resistant to penicillin G and ampicillin, but sensitive to vancomycin. Twenty hospital-acquired strains of MRSA showed a resistance to all detected antibiotics (multi-drug resistance, MDR), but vancomycin; strains of methicillin-sensitive ones, however, showed on MDR. In 45 strains of Staphylococcus aureus isolated from swabs of fingers of 62 medical workers, 31 (68.9%) were methicillin-resistant. The resistant similarity of shares of strains between medical staff and patients indicated a potential role that medical workers play in spread of nosocomial infections.

Cross Infection↗