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Functional data analysis of prosodic effects on articulatory timing.

An application of functional data analysis (FDA) (Ramsay and Silverman, 2005, Functional Data Analysis, 2nd ed. (Springer-Verlag, New York)) for linguistic experimentation is explored. The functional time-registration method provided by FDA is shown to offer novel advantages in the investigation of articulatory timing. Traditionally, articulatory studies examining the effects of linguistic variables such as prosody on articulatory timing have relied on comparing the durations of speech intervals of interest defined by kinematic landmarks. Such measurements, however, do not preserve information on the detailed, continuous pattern of articulatory timing that unfolds during these intervals. We present an approach that allows the analysis of entire, continuous kinematic trajectories obtained in a movement tracking experiment examining the influence of a phrasal boundary on articulatory patterning. FDA time deformation functions, after alignment of test and reference (control) signals, reveal delaying of articulator movement (i.e., slowing of the internal clock rate) in the presence of a phrase boundary as the speech stream recedes from the boundary. This is a theoretically predicted pattern (Byrd and Saltzman, 2003, The elastic phrase: Modeling the dynamics of boundary-adjacent lengthening, Journal of Phonetics 31, 149-180.), which would be more difficult to validate with a traditional interval-based approach. It is concluded that the FDA time alignment method provides a useful tool for characterizing timing patterns in linguistic experimentation based on continuous kinematic trajectories.

Humans↗

Dynamic specification of coarticulated German vowels: perceptual and acoustical studies.

To examine the generality of Strange's Dynamic Specification Theory of vowel perception, two perceptual experiments investigated whether dynamic (time-varying) acoustic information about vowel gestures was critical for identification of coarticulated vowels in German, a language without diphthongization. The perception by native North German (NG) speakers of electronically modified /dVt/ syllables produced in carrier sentences was assessed using the "silent-center" paradigm. The relative efficacy of static target information, dynamic spectral information (defined over syllable onsets and offsets together), and intrinsic vowel length was investigated in listening conditions in which the centers (silent-center conditions) or the onsets and offsets (vowel-center conditions) of the syllables were silenced. Listeners correctly identified most vowels in silent-center syllables and in vowel-center stimuli when both conditions included information about intrinsic vowel length. When duration information was removed, errors increased significantly, but performance was relatively better for silent-center syllables than for vowel-center stimuli. Acoustical analyses of the effects of coarticulation on target formant frequencies, vocalic duration, and dynamic spectro-temporal patterns in the stimulus materials were performed to elucidate the nature of the dynamic spectral information. In comparison with vowels produced in citation from /hVt/ syllables by the same speaker, the coarticulated /dVt/ utterances showed considerable "target undershoot" of formant frequencies and reduced duration differences between tense and lax vowel pairs. This suggests that both static spectral cues and relative duration information for NG vowels may not remain perceptually distinctive in continuous speech. Analysis of formant movement within syllable nuclei corroborated descriptions of German vowels as monophthongal. However, an analysis of first formant temporal trajectories revealed distinct patterns for tense and lax vowels that could be used by listeners to disambiguate coarticulated NG vowels.

Adult↗

Fractal characterization of bpn weights evolution.

Training methodology of the Back Propagation Network (BPN) is well documented. One aspect of BPN that requires investigation is whether or not the BPN would get trained for a given training data set and architecture. In this paper the behavior of the BPN is analyzed during its training phase considering convergent and divergent training data sets. Evolution of the weights during the training phase was monitored for the purpose of analysis. The evolution of weights was plotted as return map and was characterized by means of fractal dimension. This fractal dimensional analysis of the weight evolution trajectories is used to provide a new insight to understand the behavior of BPN and dynamics in the evolution of weights.

Algorithms↗

Impaired natural killer cell maturation in lung adenocarcinoma driven by FABP4 and SPON2 downregulation through disrupted lipid metabolism.

BACKGROUND: Although natural killer (NK) cells play a crucial role in antitumor immunity, the metabolic changes driving their dysfunction in lung adenocarcinoma remain poorly understood. This study investigates how these metabolic modifications impact NK cell function within the lung adenocarcinoma microenvironment. METHODS: A total of 13 pairs of lung adenocarcinoma samples were obtained from The Cancer Genome Atlas. Differential gene expression, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and single-cell metabolic quantification analyses were used to characterize the transcriptomic, pathway, and metabolic signatures of NK cells. The developmental trajectory was reconstructed via pseudotime analysis. The fatty acid-binding protein 4 (FABP4) and spondin2 (SPON2) expression was examined using immunofluorescence (IF) and immunohistochemistry (IHC) in patients with lung adenocarcinoma. In NK cells with FABP4 downregulation, FABP4 function was analyzed using antibody-independent cell-mediated cytotoxicity assays, flow cytometry (FCM), and liquid chromatography-mass spectrometry. RESULTS: The number of NK cells was significantly decreased in the lung adenocarcinoma microenvironment. FABP4 and SPON2 expression was significantly lower in NK cells within tumor tissues than in the adjacent tissues. FABP4 expression was significantly lower in tumor tissues than in the adjacent tissues, whereas no significant difference in SPON2 expression was observed. The cytotoxic function of NK cells with decreased FABP4 levels was impaired. Non-targeted lipid metabolism analysis indicated that differentially expressed lipids in NK cells with low FABP4 levels were functionally enriched in the glycerophospholipid metabolism pathway compared to those in normal NK cells. CONCLUSIONS: The study findings present new evidence showing that low FABP4 and SPON2 gene expression may impair NK cell maturity by affecting lipid metabolism in lung adenocarcinoma. These results provide a new perspective on restoring immune function in patients with lung cancer.

FABP4↗

Quantitative measures of performance in microvascular anastomoses.

OBJECTIVE: Methods of evaluating surgical performance are mainly subjective. This study introduces a method of evaluating surgical performance using a quantitative analysis of tool tip kinematics. METHODS: One experienced surgeon performed eight rat microvascular anastomoses over a 2-day interval. An optoelectronic motion analysis system acquired tool tip trajectories at frequencies of 30 Hz. On the basis of a hierarchical decomposition, the procedure was segmented into specific surgical subtasks (free space movement, needle placement and knot throws) from which characteristic measures of performance (tool tip trajectory, excursion and velocity) were evaluated. Comparisons of performance measures across each procedure were indexed (D scale) using the Kolmogorov-Smirnov statistic. RESULTS: Despite the marker occlusions, tool tip data were obtained 92 +/- 7% (mean +/- SD) of the time during manipulation tasks. Missing data segments were interpolated across gaps of less than 10 sample points with errors less than 0.4 mm. The anastomoses were completed in 27 +/- 4 min (range 20.5-31.4 min) with 100% patency. Tool tip trajectories and excursions were comparable for each hand, while right and left hand differences were found for velocity. Performance measures comparisons across each procedure established the benchmark for an experienced surgeon. The D-scale range was between 0 and 0.5. CONCLUSION: The study establishes a reproducible method of quantitating surgical performance. This may enhance assessment of surgical trainees at various levels of training.

Anastomosis, Surgical↗

On the convergence of the conformational coordinates basis set obtained by the essential dynamics analysis of proteins' molecular dynamics simulations.

In this article we present a quantitative evaluation of the convergence of the conformational coordinates of proteins, obtained by the Essential Dynamics method. Using a detailed analysis of long molecular dynamics trajectories in combination with a statistical assessment of the significance of the measured convergence, we obtained that simulations of a few hundreds of picoseconds are in general sufficient to provide a stable and statistically reliable definition of the essential and near constraints subspaces, at least within the nanoseconds time range. Proteins 1999;36:419-424.

Algorithms↗

Effects of phlorizin and phloretin on passive and dynamic electrical properties in muscle membrane.

The effects of phlorizin and phloretin on the cable properties were investigated in frog sartorius muscle by conventional cable analysis. Actions of phloretin on voltage-dependent ionic conductances were also studied by analysis of the phase plane trajectories. Both drugs evoked a significant decrease in specific membrane resistance (Rm) in chloride-containing Ringer's solution. The linear membrane capacitance increased by about 30%. On the contrary, in the presence of the non-penetrating anion, glutamate, a slight increase in Rm was induced by phlorizin. It is suggested that these drugs may increase the chloride conductance in the muscle membrane. Under the effect of phloretin the resting membrane potential remained unchanged but the amplitude of the action potential was lowered and the rate of repolarization was significantly reduced. The rate of depolarization during the "foot" of the action potential and the conduction velocity calculated from the rate constant of depolarization decreased. The maximum Na conductance was not altered by phloretin but K conductance was reduced. The time constant (tau K) reflecting the kinetic properties of K conductance was increased about seven-fold. It is suggested that great importance may be attributed to the dipole properties of these drugs in the actions presented above.

Action Potentials↗

An analysis of a prehistoric homicide from northern California.

An analysis was performed on a prehistoric human vertebral column recovered during an archaeological excavation in the Sierra of northern California. Fifteen burials believed to represent the Maidu culture of 400 to 700 years ago were recovered. The vertebral column from one of the individuals is particularly significant because of three embedded projectile points. The analysis attempts to recreate the trajectory of each point; to determine the possible extent of damage done by each with emphasis upon which could have been fatal; and by employing available ethnographic and archaeological data, to suggest the possible circumstances surrounding the violent event. The conclusion suggests that death may have resulted from any of the three wounds and was probably the result of an intratribal dispute.

California↗

Gunshot wounds to the buttocks.

Twenty patients sustaining gunshot wounds (GSW) to the buttocks (including one shotgun wound) were treated at two affiliated institutions during the last 5 years. Male patients predominated nine to one. Fifteen patients with extraperitoneal injuries had a benign course. One underwent negative exploratory laparotomy based upon positive physical findings. The remaining five patients had clinical abdominal findings consistent with severe intra-abdominal injuries (four organs per patient). Although the morbidity was high, there was no mortality in this series. Careful analysis of a bullet's trajectory will allow detection of a possible intraabdominal component of this type of injury, mandating early surgical management.

Abdominal Injuries↗

New measures of sperm motion. I. Adaptive smoothing and harmonic analysis.

Several kinematic measures from computer-aided sperm analysis (CASA) instruments depend critically upon the computation of the spatially averaged path of a sperm's curvilinear swimming trajectory. Presently available instruments compute the average path by smoothing the curvilinear trajectory using a fixed-length running average. We demonstrate that this method significantly distorts the spatially averaged path for irregularly swimming sperm, both within and between trajectories, resulting in inaccurate calculations for the velocity of the average path (VAP), amplitude of lateral head displacement (ALH), beat-cross frequency (BCF), wobble of the curvilinear trajectory (WOB), and straightness of the curvilinear trajectory (STR). The authors introduce an alternative approach, based on engineering signal processing techniques, where the width of the running average is adapted to the changing wavelengths of the major spatial oscillations of each curvilinear trajectory. How this adaptive method results in less distortion of the average path and produces more accurate characterizations of the above measures is shown. This method is implemented in a computer program developed by the authors, called PathTool. It is also demonstrated that the simple methods used to characterize the frequency and amplitude of the major oscillation in a sperm's curvilinear trajectory (ie, BCF and ALH) are only accurate for the most periodic, progressive, and symmetrical trajectories. Three new measures are introduced, based on mathematical harmonic analysis, that are more robust alternatives to the present methods. These new methods and measures are initially evaluated using prototypical sperm trajectories from human semen. Results suggest that adaptive smoothing and harmonic analysis produce more accurate estimates of the frequency and magnitude of oscillations in sperm trajectories than the method based on fixed-length smoothing, BCF, and ALH.

Animals↗

Chaotic dynamics of a behavior-based miniature mobile robot: effects of environment and control structure.

To study the regularity and complexity of autonomous behavior, the flow of sensory information obtained in autonomous mobile robots under various conditions was analyzed as a complex system. Sensory information time series Xn was collected from a miniature mobile robot during free navigation, and plotted on the return map, the graph of X(n+tau) vs. Xn. The plot exhibited a characteristic trajectory, representing the regularity of the time series. Correlation integral and Lyapunov exponent analysis also showed properties of deterministic chaos; the presence of fractal dimension and positive Lyapunov exponent. Analysis of sensory information obtained in the robot with three different neural controllers revealed that the autonomous robot behaves in such a way that the flow of sensory information is governed by a deterministic rule, and this pattern is unique to each controller. Furthermore, the analysis in various environments exhibited that transitions from one trajectory to another on the return map occur during the course of autonomous behavior. The fractal and Lyapunov dimensions calculated in various conditions indicate that these dimension could be utilized to quantify the complexity of autonomous behavior and the relative difficulty of tasks. Analyses at different evolutionary stage revealed that behavioral performance correlates with fractal dimension. These studies using a miniature mobile robot that allowed to idealize the experimental conditions demonstrated firmly that the complex analysis could be utilized in evaluation and optimization of autonomous systems and the behavior.

Behavior↗

Effects of head extension on undisturbed upright stance control in humans.

The purpose of the present experiment was to investigate whether and how the head extended posture, commonly encountered in many routine activities, affects undisturbed upright stance control mechanisms in humans. Sixteen young healthy adults stood feet together, with their eyes closed and were asked to sway as little as possible in two Neutral and Extended head conditions. Centre of pressure (CP) displacements, recorded using a force platform, were used to compute the motions of the vertical projection of the centre of gravity (CG(v)) and those of the difference CP - CG(v). A time-domain analysis shows increased mean velocity and surface covered by the trajectory of both elementary motions in the Extended head condition. A frequency analysis also reveals increased root mean squares on the CP - CG(v) motions, suggesting increased muscular activity in the Extended head condition. Furthermore, similar changes occur on CG(v) motions. Finally, modelling these trajectories as a fractional Brownian motion process demonstrates increased spatial transition point co-ordinates at which the corrective process is initiated and a more deterministic control mechanism in this corrective process involving CG(v) motions in the Extended head position. Together, the present findings suggest that head extension position yields a reorganisation of the control mechanisms for maintaining undisturbed upright stance.

Adult↗

Comparing personal trajectories and drawing causal inferences from longitudinal data.

This review considers statistical analysis of data from studies that obtain repeated measures on each of many participants. Such studies aim to describe the average change in populations and to illuminate individual differences in trajectories of change. A person-specific model for the trajectory of each participant is viewed as the foundation of any analysis having these aims. A second, between-person model describes how persons very in their trajectories. This two-stage modeling framework is common to a variety of popular analytic approaches variously labeled hierarchical models, multilevel models, latent growth models, and random coefficient models. Selected published examples reveal how the approach can be flexibly adapted to represent development in domains as diverse as vocabulary growth in early childhood, academic learning, and antisocial propensity during adolescence. The review then considers the problem of drawing causal inferences from repeated measures data.

Adolescent↗

A comparison of girls' and boys' aggressive-disruptive behavior trajectories across elementary school: prediction to young adult antisocial outcomes.

Multiple group analysis and general growth mixture modeling was used to determine whether aggressive- disruptive behavior trajectories during elementary school, and their association with young adulthood antisocial outcomes, vary by gender. Participants were assessed longitudinally beginning at age 6 as part of an evaluation of 2 school-based preventive programs. Two analogous trajectories were found for girls and boys: chronic high aggression- disruption (CHAD) and stable low aggression- disruption (LAD). A 3rd class of low moderate aggression- disruption (LMAD) for girls and increasing aggression- disruption (IAD) for boys also was found. Girls and boys in analogous CHAD classes did not differ in trajectory level and course, but girls in the CHAD and LAD classes had lower rates of antisocial outcomes than boys. Girls with the LMAD trajectory differed from boys with the IAD trajectory.

Achievement↗

Cumulative microscopy reveals cellular states in fibroblasts from patients with genetic disorders.

Analysis of cellular states and signaling trajectories can provide insights into causes of disease. We developed cumulative microscopy, a method to perform cyclical imaging without elution or quenching steps. Cumulative microscopy computationally extracts individual signals from accumulating fluorescence during sequential imaging. We use cumulative microscopy to quantitatively assess cell cycle and stress markers in individual primary fibroblasts from patients with rare genetic proliferative disorders with increased cancer risk. Neural network-based analysis of cumulative microscopy data suggests that cells from patients with Cartilage-hair hypoplasia (CHH), but not Mulibrey Nanism (MUL), show replication stress. We analyze cell states and cell trajectories and find that a subset of cells from patients with CHH show spontaneous replication stress, followed by cell cycle exit in both G1 and G2 phases. We note that replication stress potentially could underlie both proliferative defects and increased cancer risk in CHH patients and conclude that cumulative microscopy is an efficient, quantitative, and generalizable approach to multiplex microscopy.

Humans↗

Use of the anatomic trajectory for thoracic pedicle screw salvage after failure/violation using the straight-forward technique: a biomechanical analysis.

STUDY DESIGN: A biomechanical study of cadaver vertebrae to determine the feasibility of obtaining adequate thoracic pedicle screw fixation in a salvage situation. OBJECTIVE To investigate the ability to safely place a thoracic pedicle screw with adequate maximal insertional torque (MIT) using the anatomic trajectory (AT) (directed along the true anatomic axis of the pedicle) after purposeful failure/medial violation of the pedicle using the straight-forward trajectory (ST) (paralleling the vertebral endplate). SUMMARY AND BACKGROUND DATA: Failure to place a pedicle screw at an intended segment of the thoracic spine may theoretically render the level uninstrumented, because pedicle hook placement may be unsafe and the transverse process may be fractured. An alternative pedicle screw insertion technique, if biomechanically sound in this situation, may present an excellent alternative for critical instrumentation levels. MATERIALS AND METHODS: Fixed-head 5.0 mm stainless steel pedicle screws were placed using the ST and MIT was recorded after determination of bone mineral density (BMD) with dual-energy radiograph absorptiometry (DEXA) scanning. Purposeful screw malposition and critical pedicle wall failure were performed, followed by salvage placement of the screw using the AT. Insertional torque was recorded for each screw revolution with a digital torque wrench and MIT was again recorded to compare the MIT obtainable in the salvage situation. RESULTS: BMD for the vertebrae averaged 732 g/cm2 (620-884 g/cm2). The MIT for the straight-forward technique without pedicle violation was 2.61 +/- 0.19 (SE) inches per pound, whereas that of the salvage procedure after medial wall violation (AT) averaged 1.62 +/- 0.12 (SE) inches per pound. Therefore, the AT achieved 62% (P = 0.027) of the fixation strength (in terms of MIT) during salvage after failure/medial violation of the pedicle. MIT for both the ST* and AT trajectories correlated with both global BMD of the vertebrae (*P = 0.008; P = 0.004) and regional BMD of the vertebral body (*P = 0.044; P = 0.023). CONCLUSIONS: The AT achieved 62% (P = 0.027) of the MIT during salvage of a failed/violated pedicle. BMD correlated with both the initial and salvage techniques. The AT provides adequate fixation in a salvage situation and may be safely used to provide segmental fixation at critical levels.

Absorptiometry, Photon↗

Premorbid functioning trajectories and the one-year course of cognitive performance in first-episode psychosis: a cluster analysis in PSYSCAN.

BACKGROUND: We examined the course of cognitive performance in first-episode psychosis (FEP) compared to healthy controls (HC), and whether this varied across subgroups of patients defined by premorbid functioning (PMF) trajectories, using a clustering approach. METHODS: Data were collected in 302 FEP and 136 HC subjects participating in PSYSCAN (HEALTH.2013.2.2.1-2-FEP). K-means clustering (Euclidean distance) was used to cluster longitudinal trajectories of different PMF domains simultaneously. Since PMF was assessed retrospectively using the Premorbid Adjustment Scale (PAS), findings should be interpreted with caution, although PAS ratings have shown reasonable validity against prospective data. RESULTS: As expected, FEP showed impaired performance across all cognitive domains compared to HC. We identified four trajectories of PMF: a normal premorbid developmental trajectory (globally-normal, 21 %), stable intermediate PMF across domains (stable-intermediate, 29 %), stable poor or deteriorating PMF in the academic domain (normal-social/poor-academic, 29 %), and a globally impaired group with poor/deteriorating PMF across domains (globally-poor, 21 %). These clusters showed distinct levels of post-onset impairments in sustained visual attention, visual working memory and emotion recognition. CONCLUSIONS: This study confirms a positive association between PMF and cognitive performance in the early years following psychosis onset. It aligns with findings that individuals later diagnosed with schizophrenia already show developmental deficits/lags from childhood to early adolescence compared to normally developing children. As PMF can be considered a proxy for cognitive reserve, our results suggest that higher reserve acts as a buffer against cognitive decline and supports better performance on sustained visual attention, complex visual working memory, and aspects of emotion recognition.

Clustering↗

Examining early-phase symptom trajectories in interpersonal psychotherapy versus antidepressant medication for adults with depression: A dynamic time warp network analysis.

BACKGROUND: Depression is characterized by substantial symptom heterogeneity, which is often concealed when examining total severity scores. Analyzing symptom-level change can improve our understanding of treatment effects and recovery processes. This study, therefore, examined dynamic symptom networks during early-phase interpersonal psychotherapy (IPT) and selective serotonin reuptake inhibitor (SSRI) antidepressant treatment, assessing patterns of symptom change across as well as differences between treatments. METHODS: Using weekly item-level Hamilton Depression Rating Scale (HAM-D) data from a randomized clinical trial comparing IPT and SSRIs for adults with depression, this preregistered study examined symptom trajectories in the first six weeks of treatment with Dynamic Time Warping (DTW). RESULTS: Depressive symptom trajectories and DTW-based symptom networks were largely similar for IPT and SSRI. In both conditions, changes in somatic symptoms of anxiety and middle insomnia tended to precede improvements in depressed mood. CONCLUSIONS: Early symptom change may occur outside the core affective domain, underscoring the importance of monitoring symptoms broadly. Symptom-level patterns may reflect patients' stage of recovery and provide clinically relevant information beyond total severity scores. The absence of differences in improvement patterns between IPT and SSRI suggest few indications for treatment selection based on baseline symptom profiles. Future research should replicate and extend these findings to subsequent treatment phases using more frequent assessments and a broader range of interventions.

Humans↗