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PubMed2000

A Hermaphrodite Molecule: Quantitative Copper(I)-Directed Formation of a Doubly Threaded Assembly from a Ring Attached to a String We are grateful to Jean-Daniel Sauer for high-field NMR experiments and to Raymond Hubert and Hélène Nierengarten for MS measurements. We also gratefully acknowledge the help of Prof. Jean Fischer and Dr. Nathalie Kyritsakas (Laboratoire de Cristallochimie et de Chimie Structurale, Université Louis Pasteur). M.C.J. thanks the European Commission for a Grant (TMR Contract No. ERBFMBICT972547).

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PubMed2000

Dimerization of an Inactive Fragment of Huperzine A Produces a Drug with Twice the Potency of the Natural Product This work was supported by the Research Grants Council of Hong Kong (HKUST6156/97M), the Biotechnology Research Institute (HKUST), the Mayo Foundation, and the Istituto Pasteur Fondazione Cenci Bolognetti (E.P.). We thank Prof. X. C. Tang (Shanghai Institute of Materia Medica) for a gift of (-)-1.

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PubMed2000

The intraperitoneal Plasmodium berghei-Pasteur infection of Swiss mice is not a system that is able to detect the antiplasmodial activity in the Pothomorphe plant extracts that are used as antimalarials in Brazilian endemic areas.

The antimalarial activity of the hexane and methanol extracts derived from the Brazilian plants Pothomorphe peltata and Pothomorphe umbellata-whose leaves are popularly employed in medicinal folk remedies for the treatment of malaria-was assessed through in vivo tests with the Peters method. The extracts were delivered to Plasmodium berghei-infected mice via the oral or the subcutaneous route. A suppressive effect on the parasitemia seemed to be evident when data regarding the intraperitoneal injection of Pothomorphe umbellata extracts were analyzed. However, a definitive conclusion on an effective antimalarial activity is not possible, as two distinct-"standard" and "slow"-patterns of parasitemia occurring at similar frequencies in both treated and untreated intraperitoneally infected mice were observed. Nevertheless, the existence of two distinct profiles of parasitemia was not clear among the animals that were infected via the intravenous route. These data indicate the need for further studies on the biological features of the host/parasite interaction in the intraperitoneally P. berghei-infected Swiss mice system to standardize the model and to improve its usefulness in the screening of antimalarial compounds.

Animals↗
PubMed1988

Analysis of triazine herbicides residues in butter and pasteurized milk.

A method has been developed for the determination of atrazine, cyanazine, prometryn, simazine, and terbutryn residues in butter. The residues were extracted from the matrix with a mixture of petroleum ether/methanol (3 + 1), and from the separated water-methanol phase extraction was carried out with chloroform. The extract was cleaned up on an alumina column. Capillary glass liquid chromatography using a 15 m x 0.32 mm glass capillary column coated with OV-1 and an alcali flame ionization detector were employed for the analysis of the residues. The analyses were evaluated by the internal standard method, using metribuzin as the internal standard. The recovery of the method was 68.7%-79.8% for the individual herbicides under study at the fortification level of 0.1 mg.kg-1 and 79.2%-91.9% at the fortification level of 0.02 mg.kg-1. The determination limit of the method was 0.005 mg.kg-1. When centrifuging full milk, residues of triazines were partitioned between the water and fat phases, whereby 17%-82% of the residues were transferred to the milk fat. Samples of commercial butter were analysed and found to contain 0.005-0.023 mg.kg-1 atrazine.

Animals↗