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A novel neuropeptide precursor gene is expressed in the terrestrial snail central nervous system by a group of neurons that control mating behavior.

We report the isolation of a cDNA clone encoding a neuropeptide precursor named preproGFAD from the central nervous system (CNS) of the snail Helix lucorum. Analysis of the expression of this gene shows that it is neurospecific and expressed in several groups of CNS neurons. Most notable is the expression of preproGFAD gene in the right mesocerebrum, where the neurons controlling mating behavior are located. The expression in this particular region is observed in adult animals but not in juvenile ones. The preprohormone is 108 amino acids long and contains a hydrophobic leader peptide and eight Lys-Arg recognition sites for endoproteolysis. The post-translational processing of the prohormone may lead to the generation of seven tetrapeptides, Gly-Phe-Ala-Asp-COOH (GFAD). This peptide has the same sequence as two previously isolated peptides from a related snail, Achatina fulica. The first of them (achatin-I) contains D-Phe; the second (achatin-II) is its L-Phe-containing stereoisomer. Injection of synthetic D-GFAD in nanomolar concentrations into intact animals caused an increase of the heartbeat rate and opening of the genital atrium. In preparations containing CNS with intact innervation of reproductive organs, bath application of D-GFAD caused extensive movements of the penis but not of other reproductive organs. Intracellular activation of individual neurons expressing the preproGFAD gene also elicited penis movements. D-GFAD also suppressed activity of neurons modulating feeding behavior. Our data therefore indicate that the preproGFAD gene encodes the precursor of a neuropeptide that participates in the regulation of male mating behavior.

Amino Acid Sequence↗

Control of behavior by an establishing stimulus.

Seventeen pigeons were exposed to a three-key discrete-trial procedure in which a peck on the lit center key produced food if, and only if, the left keylight was lit. The center key was illuminated by a peck on the lit right key. Of interest was whether subjects pecked the right key before or after the response-independent onset of the left keylight. Pecks on the right key after left-keylight onset suggest control of behavior by the left keylight-an establishing stimulus. In three experiments, the strength of center-keylight onset as conditioned reinforcer for a response on the right key was manipulated by altering the size of the reduction in time to food delivery correlated with its onset. Control of pigeons' key pecks by onset of the left keylight occurred on more trials per session when the center keylight was a relatively weak conditioned reinforcer and on fewer trials per session when the center keylight was a relatively strong condtioned reinforcer. Differences across conditions in the degree of control by onset of the establishing stimulus were greatest when changes in conditioned reinforcer strength occurred relatively frequently and were signaled. The results provide evidence of the function of an establishing stimulus.

Journal Article↗

Weight-related issues and high-risk sexual behaviors among college students.

Individuals with high body mass index (BMI), negative body image, and unhealthy weight control behaviors may be more likely to engage in high-risk sexual behaviors than others. Researchers have not fully investigated these relationships in a sample of college students. The authors collected data regarding several weight-related issues and high-risk sexual behaviors (response rate = 39%) from 1,168 college students. Over half reported at least 1 high-risk sexual behavior. After the authors controlled for several demographic characteristics, they found that higher BMIs were associated with increased odds of having a casual sexual partner (odds ratio [OR] = 2.70) and being intoxicated at last intercourse (OR = 2.25). Among women, unhealthy weight control behaviors were associated with having a casual partner (OR = 2.12), using no or unreliable contraception (OR = 1.98), and being intoxicated at last intercourse (OR = 2.31). Weight-related issues were not associated with sexual behaviors among college men. The authors describe their findings within two health behavior frameworks, and they discuss implications for research and health education.

Adolescent↗

Validation studies of a multifactor cognitive-behavioral Anger Control Inventory.

Two studies are reported on a new Anger Control Inventory based on a cognitive-behavioral person-situation interaction model. In Study 1, the inventory responses of 118 clinical subjects and 190 normal subjects were correlated with an anger problem criterion based on observable signs. Factor analyses were conducted to clarify the construct base of the inventory. In Study 2, comparisons were made between the inventory responses of 100 spouse batterers and 96 normal subjects. Significant differences between the pretreatment and posttreatment scores of 65 batterers given treatment in anger control were obtained on the inventory scales, which were found to be correlated with the problem criterion and discriminating between the clinical and normal groups. The construct validity and clinical utility of the Anger Control Inventory are discussed.

Adaptation, Psychological↗

A TRS-80-based system for the control of behavioral experiments.

A TRS-80-based system is described for controlling and recording events from operant chambers. The system features a TRS-80 microprocessor with 32 K RAM, a floppy disk drive, an LVB optical interface, a printer, and a user-oriented program (Operant Protocol System, OPS). The LVB interface system is capable of up to 128 independent input/output operations for each of up to 8 boxes. The system uses assembly language routines for real-time control and event recording of up to 8 boxes simultaneously. BASIC is used to obtain user input and deliver data output in an interactive fashion. Advantages include dependable hardware, extensively documented and tested software, relative inexpensiveness, and standard-deviation-based variable schedules.

Animals↗

The critical role of nucleus accumbens dopamine systems in the mediation of fixed interval schedule-controlled operant behavior.

Microinjections of the irreversible dopamine antagonist EEDQ (N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline), into nucleus accumbens but not into dorsal striatum, markedly decreased response rates on a fixed interval schedule of reinforcement. These preferential effects in nucleus accumbens could be prevented by selectively activating either D1 or D2 receptors. These data demonstrate a critical role for mesolimbic dopamine systems in the mediation of fixed interval schedule-controlled operant behavior.

Animals↗

Psychosocial correlates of physical activity in white and African-American girls.

PURPOSE: To evaluate the relative utility of the Theory of Reasoned Action (TRA) and the Theory of Planned Behavior (TPB) in explaining intentions and physical activity behavior in white and African-American eighth-grade girls. METHODS: One-thousand-thirty white and 1.114 African-American eighth-grade girls (mean age 13.6 +/- 0.7 years) from 31 middle schools in South Carolina completed a 3-day physical activity recall and a questionnaire assessing attitudes, subjective norms, perceived behavioral control, self-efficacy, and intentions related to regular participation in moderate-to-vigorous physical activity (MVPA). RESULTS: Among Whites, 17% of the variance in intentions was contributed by subjective norms and attitude, with intentions accounting for 8% of the variance in MVPA. The addition of perceived behavioral control and self-efficacy to the TRA significantly improved the prediction of intentions and MVPA accounting for 40% and 10% of the variance, respectively. Among African-Americans, subjective norms and attitude accounted for 13% of the variance in intentions, with intentions accounting for only 3% of the variance in MVPA. The addition of perceived behavioral control and self-efficacy to the TRA significantly improved the prediction of intentions and MVPA accounting for 28% and 5% of the variance, respectively. CONCLUSIONS: The results provided limited empirical support for the TPB among white adolescent girls; however, our findings suggest that the planned behavior framework has limited utility among African-American adolescent girls. The relatively weak link between intentions and MVPA observed in both population groups suggest that constructs external to the TPB may be more important mediators of physical activity behavior in adolescent girls.

Adolescent↗

Utility of the theory of reasoned action and theory of planned behavior for predicting Chinese adolescent smoking.

One third of smokers worldwide live in China. Identifying predictors of smoking is important for prevention program development. This study explored whether the Theory of Reasoned Action (TRA) and Theory of Planned Behavior (TPB) predict adolescent smoking in China. Data were obtained from 14,434 middle and high school students (48.6% boys, 51.4% girls) in seven geographically varied cities in China. TRA and TPB were tested by multilevel mediation modeling, and compared by multilevel analyses and likelihood ratio tests. Perceived behavioral control was tested as a main effect in TPB and a moderation effect in TRA. The mediation effects of smoking intention were supported in both models (p<0.001). TPB accounted for significantly more variance than TRA (p<0.001). Perceived behavioral control significantly interacted with attitudes and social norms in TRA (p<0.001). Therefore, TRA and TPB are applicable to China to predict adolescent smoking. TPB is superior to TRA for the prediction and TRA can better predict smoking among students with lower than higher perceived behavioral control.

Adolescent↗

The evolution of neural circuits controlling feeding behavior in frogs.

Our approach to understanding motor systems is a phylogenetic, 'outside-in' approach, the goal of which is to identify behavioral transitions during phylogenesis and elucidate their neurological basis. In this paper, we review the results of recent behavioral, biomechanical and neurological studies on frog feeding behavior. These studies show that highly protrusible tongues have evolved numerous times independently among frogs, and that the biomechanics and neuromuscular control of feeding behavior have been transformed repeatedly during frog evolution. Many of the independent lineages possess unique biomechanical mechanisms for protracting their tongues and unique neural mechanisms for coordinating feeding behavior. In frogs, there has been considerable evolution at the interface between reticular central pattern generators (CPGs) associated with feeding and sensory feedback circuits that modulate feeding motor output. In particular, the roles of hypoglossal and glossopharyngeal sensory feedback appear to have been relatively plastic in their evolution. Prey-type dependence of hypoglossal sensory feedback in Rana suggests that the interaction between descending visual control and sensory feedback also may be evolutionarily plastic. Comparative studies have found that motor systems sometimes evolve conservatively across morphological and behavioral transitions (i.e., the shoulder in birds) or, alternatively, they may be subject to considerably more evolutionary change than is reflected in morphological characteristics (i.e., feeding in cichlids). We hypothesize that the CPG circuits for feeding behavior in the reticular formation may evolve conservatively because they are highly integrated, multifunctional networks which cannot be optimized for one function without compromising others. In contrast, the interfaces between the CPG, sensory feedback and descending control should be less constrained. When changes in motor patterns occur during evolution, it is likely that sensory feedback or descending control may be involved.

Animals↗

Locomotor behavior and control in human and non-human primates: comparisons with cats and dogs.

For many years the cat has been the accepted mammalian model for investigations on the neural control of locomotion. The results from such studies, and also similar studies on dogs, have been assumed to represent the typical mammalian condition. The primary purpose of this review is to evaluate this assumption relative to human and non-human primates. A second purpose is to acquaint investigators of mammalian locomotor behavior and control with the large amount of data available on this topic for non-human primates. The analysis shows that non-human primates are different from carnivores in footfall patterns, gaits, gait transitions, relative stride length, limb angular excursions, weight support, mechanisms of propulsion, spinal vs. supraspinal control of stepping, and possible EMG patterns. Humans exhibit more similarities with other primates than with cats or dogs, but also appear to be unique in many ways. Thus, it is clear that extrapolations of results based on cat or dog experiments may not be applicable to non-human or human primates. Furthermore, although non-human primates unquestionably make a better experimental model than cats or dogs for understanding human locomotor control mechanisms, exactly how much better remains to be determined.

Animals↗

Predicting intentions to obtain a Pap smear among African American and Latina women: testing the theory of planned behavior.

OBJECTIVE: To determine the empirical adequacy of the theory of planned behavior (TPB) to explain Pap smear use intentions in African American and Latina women. METHOD: A correlational design was used, and a convenience sample of 108 African American and 96 Latina adult women were recruited from urban community-based agencies located in a large mid-Atlantic metropolitan area. The Pap Smear Questionnaire (PSQ) was designed and used. The Demographic Assessment Survey collected demographic information (age and socioeconomic status for both groups; and level of acculturation for the Latinas). RESULTS: Direct relationships between attitude and perceived behavioral control and intention to obtain an annual Pap smear were found for African American and Latina women. The subjective norm did not significantly predict intention. Attitude (beta = .58; p < .001) provided the best explanation of intention among African American women to obtain an annual Pap smear, followed by perceived behavioral control (beta = .30; p < .001). Among Latinas, the findings reflected those of the African American sample. However, attitude (beta = .40; p < .001) and perceived behavioral control (beta = .35; p < .001) were weighted similarly. The external variables of age and income had indirect effects on intention for African American and Latina women, respectively. CONCLUSION: The study findings did not support the empirical adequacy of the TPB for either of the ethnic groups. Future studies should test a modified version of the TPB that includes measures of both social support and subjective norms. Direct measure items of subjective norm, group-specific measures of perceptions of control, and other measures of acculturation should be added to the PSQ and further tested.

Adolescent↗

Cognitive mediators of the social influence-exercise adherence relationship: a test of the theory of planned behavior.

The purpose of this study was to examine cognitive constructs from the theory of planned behavior (i.e., attitude, perceived behavioral control, and intention) as potential mediators of the relationship between selected social influence constructs (i.e., subjective norm, social support, and cohesion) and adherence to structured exercise classes. Sixty-two participants completed self-administered questionnaires during the fourth week (social influence constructs) and eighth week (cognitive constructs) of a 12-week exercise program. Exercise adherence was monitored during weeks 9 through 12 using perceived intensity and attendance. Pearson correlations indicated that social support correlated with perceived behavioral control, whereas cohesion correlated with attitude. Path analysis supported two distinct paths from social influence to exercise adherence: (a) social support-->perceived behavioral control-->intention-->exercise adherence, and (b) cohesion-->attitude-->intention-->exercise adherence. Discussion focuses on the theoretical importance of these findings, conceptual and measurement issues regarding subjective norm, and suggestions for future research.

Adult↗

Maternal and paternal parenting, acculturation, and young adolescents' psychological adjustment in Korean American families.

PROBLEM: Maintaining healthy psychological adjustment may be more complicated for Korean American young adolescents than for their European American counterparts. Korean American families live in two cultures and the issue of acculturation challenges their parenting, which is a critical factor for young adolescents' psychological adjustment. METHODS: Hierarchical multiple regressions were used to analyze 106 family data (young adolescents, mothers, and fathers) on parenting (acceptance-rejection and behavioral control), acculturation (integration, assimilation, separation, and marginalization), and young adolescents' psychological adjustment. FINDINGS: Only young adolescents' reports of maternal and paternal acceptance-rejection were related to their report of psychological adjustment. However, maternal acculturation moderated the relation between mothers' reports of maternal acceptance-rejection and behavioral control and young adolescents' report of their psychological adjustment. CONCLUSIONS: Parental acceptance-rejection is related to young adolescents' psychological adjustment when adolescents perceive themselves as accepted or rejected by parents. The relation between mothers' report of maternal acceptance-rejection and behavioral control and adolescents' psychological adjustment needs to be considered within the context of maternal acculturation.

Acculturation↗

Does dieting lead to weight gain? A four-year longitudinal study of middle school girls.

OBJECTIVE: A majority of the published longitudinal research on children has reported that dieting is related to weight gain at a later point in time. The purpose of this study was to look at weight control behaviors and patterns of weight gain and loss, specifically whether dieting is related to weight gain. RESEARCH METHODS AND PROCEDURES: Baseline data were collected from 1358 female students in grades 6 to 9 from schools in Hayward, CA, and Tucson, AZ. Data were obtained annually over a 4-year period. Paper-and-pencil questionnaires and height and weight were obtained during the students' regular classroom periods. Dieting was measured both with the single item, "In the past year, how often have you been on a diet to lose weight?" scored from "never" to "always," and with a Dieting Behavior Scale including five items on weight control behaviors. Changes in BMI z-scores were analyzed. RESULTS: On average, girls who reported "never" dieting were most likely to have an increased BMI z-score at the next measurement, and those who reported "always" dieting were most likely to have a decreased BMI z-score. The same pattern was true for the Dieting Behavior Scale. DISCUSSION: Our finding that dieting and weight gain were not related, independently of initial BMI, does not mean that dieting to lose weight is appropriate, especially among young girls. Additional research is needed both to examine this relationship and to determine exactly what behaviors children are engaging in when they report that they are dieting.

Adolescent↗

Stimulus control of behavioral history.

Pigeons were exposed to two different reinforcement schedules under different stimulus conditions in each of two daily sessions separated by 6 hr (Experiments 1 and 2) or in a single session (Experiment 3). Following this, either a fixed-interval (Experiment 1) or a variable-interval schedule (Experiments 2 and 3) was effected in both stimulus conditions. In the first two experiments, exposure to fixed-ratio or differential-reinforcement-of-low-rate schedules led to response-rate, but not pattern, differences in subsequent performance on fixed- or variable-interval schedules that persisted for up to 60 sessions. The effects of reinforcement-schedule history on fixed-interval schedule performance generally were more persistent. In Experiment 3, a history of high and low response rates in different components of a multiple schedule resulted in subsequent response-rate differences under identical variable-interval schedules. Higher response rates initially occurred in the component previously correlated with high response rates. For 3 of 4 subjects, the differences persisted for 20 or more sessions. Previous demonstrations of behavioral history effects have been confined largely to between-subject comparisons. By contrast, the present results demonstrate strong behavioral effects of schedule histories under stimulus control within individual subjects.

Animals↗

Trial number and compound stimuli temporal relationship as joint determinants of second-order conditioning and conditioned inhibition.

Two conditioned lick suppression experiments with rats used feature-negative training (A-footshock trials intermixed with nonreinforced XA presentations) to analyze the role of the number of XA compound presentations and the temporal relationship of the elements within the compound (simultaneous or serial) as determinants of the resulting behavioral control. Second-order conditioning (i.e., excitatory behavioral control by X) was observed to decline as the number of XA compound trials was increased. This decline was more rapid if X and A were presented simultaneously, as opposed to serially (i.e., X before A; Experiment 1). Conditioned inhibition to X, as assessed by a summation test (Experiment 1) and a retardation test (Experiment 2), increased with the number of XA trials and did so more quickly for simultaneous than for serial pairings of X and A. The results help to clarify previously discrepant findings regarding factors that promote excitation versus inhibition with this protocol.

Animals↗

Can the theory of planned behavior predict the maintenance of physical activity?

This study tested the ability of the theory of planned behavior to predict actual participation in physical activity and explored the development of activity habits in a 12-week longitudinal study. People enrolling in a gymnasium (N = 94) completed standard theory of planned behavior measures at baseline and follow-up; behavior was monitored objectively in the intervening period. The data were analyzed by using both standard and repeatable events survival analysis. Results showed that (a) perceived behavioral control was significantly predictive of intentions and actual behavior, (b) stable exercise habits developed in the first 5 weeks of the study, and (c) successful prior performance enhanced perceptions of behavioral control. The implications for developing theory-based interventions that promote the maintenance of health behavior are discussed.

Adult↗

The theory of planned behavior as related to intention to exercise and exercise behavior.

In a previously reported study we sought to assess the usefulness of the theory of planned behavior in explaining the exercise behavior of 73 adults enrolled in an exercise program over a 5-mo. period. The correlation between scores on Intention to Exercise and Exercise Behavior was moderate and inverse, conflicting with a primary tenet of the Theory of Planned Behavior. In this analysis of the data we sought to explain that finding by partitioning participants into groups based on a median split of their scores on Intention to Exercise and Exercise Behavior. Using the four Intention by Behavior groups as independent variables and scale scores as dependent variables, post hoc tests for Fitness Attitude showed significant differences only when High Intention conflicted with Low Intention, irrespective of Exercise Behavior. Perceived Behavioral Control showed opposite tendencies, i.e., differences were significant when High Behavior conflicted with Low Behavior, irrespective of Intention to Exercise. We conclude that Perceived Behavioral Control defines one's ultimate Exercise Behavior over a 5-mo. period while scores on Fitness Attitude define Intentions to Exercise.

Adult↗