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At least 613 records · Page 34Linked to original sources
PubMed1980

Application of gel permeation chromatography and nonaqueous reverse phase chromatography to high pressure liquid chromatographic determination of retinyl palmitate in fortified breakfast cereals.

A high pressure liquid chromatographic (HPLC) method was developed for determing retinyl palmitate in cereals. Retinyl palmitate is fractionated from other methylene chloride-soluble components by using high pressure gel permeation chromatography (HP-GPC) followed by quantitation with nonaqueous reverse phase (HPLC (RP-HPLC). HP-GPC fractionation was accomplished using 2 microStyragel (100A) columns connected in series on sample extracts in methylene chloride with methylene chloride as the mobile phase. A valve designed to facilitate the collection of the vitamin A fraction was installed in-line between the refractive index and absorbance detectors. RP-HPLC quantitation was achieved on microBondapak C18 (10 micrometers) using methylene chloride-acetonitrile (30 + 70). Based on 23 repetitive analyses, recovery of vitamin A as retinyl palmitate in cereal products was 95.4 +/- 4.2% with spiking levels between 21.4 and 140 micrograms/g. Vitamin A in 15 cereals, representing bran, corn, oats, rice, and wheat, ranged from 34 to 194% of the declared level. The HPLC procedure was compared with the AOAC official colorimetric method and with an ultraviolet spectrophotometric method.

Chromatography, Gel↗
PubMedDate not supplied

The cholesterol-lowering effect of a breakfast cereal containing psyllium fibre.

OBJECTIVE: To determine the cholesterol-lowering effect of ready-to-eat cereal containing soluble fibre as psyllium (86%), oatmeal and barley on the plasma lipids of otherwise healthy men with mild hypercholesterolaemia, who were already eating a diet low in saturated fats. DESIGN: Double-blind crossover trial, lasting 12 weeks, in volunteers eating each cereal for six weeks at home, in Sydney and Newcastle (New South Wales). PARTICIPANTS: Eighty-one men (average age 50 years; range, 31-69 years) who had initial plasma (total) cholesterol concentrations of 5.8-8.8 mmol/L, but did not have major illness, obesity or diabetes, and were not on special diets or taking stool bulkers. INTERVENTION: One box of cereal was eaten each day: control, 60 g wheat/wheat bran (2 g soluble fibre); or test, 50 g of product containing psyllium/oats/barley (12 g soluble fibre). Subjects were allocated at random, when established on a low saturated fat diet, to wheat followed by psyllium cereal or psyllium followed by wheat cereal. MAIN OUTCOME MEASURES: Plasma total cholesterol, high density lipoprotein cholesterol, triglyceride and low density lipoprotein cholesterol concentrations measured twice, four to seven days apart before the start of the trial and then after six weeks of eating each cereal. RESULTS: Compliance was excellent with both cereals. There were no significant differences in subjects' macronutrient intake or body weight between the two six-week periods. Total cholesterol and low density lipoprotein cholesterol concentrations fell significantly on psyllium cereal, relative to wheat cereal, in both periods at both centres (mean -3.2% and -4.4%, respectively). There were no consistent changes in triglyceride or high density lipoprotein cholesterol concentrations. CONCLUSIONS: This type of product, which is easy to consume on a daily basis, is a useful adjunct to the dietary management of mild hypercholesterolaemia.

Adult↗
PubMed1977

Breakfast in bed?

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