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Effect of cortisol on luteinizing hormone release during sexual behavior in the boar.

The effect of inhibiting the rise in cortisol concentrations that occurs at copulation upon luteinizing hormone release was studied in seven adult boars. Plasma samples were collected for assay of luteinizing hormone, testosterone and cortisol on a control day and before, during and after exposure to an estrous sow. The area under the curve was used to evaluate hormone production and treatment effects were evaluated by a paired Student's t-test. The 11 beta-hydroxylase inhibitor, metyrapone, was used to suppress glucocorticoid hormone production. Cortisol concentrations increased significantly (p less than 0.05) after breeding compared to values on the control day while treatment with metyrapone prior to breeding prevented the cortisol increase (p greater than 0.05). Although luteinizing hormone production increased significantly after copulation in both breeding experiments, metyrapone pretreatment resulted in a reduction of luteinizing hormone secretion (p less than 0.05). Testosterone production was also reduced in boars pretreated with metyrapone. The results suggest that the increased levels of cortisol occurring at copulation may enhance luteinizing hormone release in boars.

Animals↗

Influence of sex on the resistance of sheep lambs to an experimental Haemonchus contortus infection.

36 intact male and 69 female lambs of two breeds (n = 63 Merinoland; n = 42 Rhön) aged 12 weeks were orally infected with 5000 infective-stage larvae, L3, of the nematode Haemonchus contortus. After 4 and 8 weeks faecal egg counts (FEC), haematocrit values and plasma testosterone levels were determined. All lambs were slaughtered at 20 weeks of age. The gastrointestinal tracts were examined for the presence of adult stages of H. contortus. Male lambs showed significantly higher log FEC (p < 0.001), higher mean establishment rates (p < 0.05), higher worm burdens (p < 0.01) and lower haematocrit values (p < 0.001) when compared with female lambs. Correlations between economically important traits (body weight, daily weight gain) and parasitological parameters were significantly higher in male animals. Testosterone level was 4 weeks after infection significantly positive correlated with worm burden. The results suggest that female lambs are more resistant against an experimental H. contortus infection when compared with male lambs. Testosterone seems to play an important role in resistance. This approach can be of importance if parasite resistance is incorporated into breeding programs and the estimated breeding values for rams are only based on male offspring information. Therefore male breeding values are probably not representative for the whole population.

Animals↗

The value of embryo transfer to cattle breeding in Britain.

An analysis is made of the maximum expenditure which could be justified in embryo transfer in cattle is used to: increase the rate of genetic improvement of dairy or beef cattle; increase the frequency of twin-pregnancies; and expedite a change of breed. Estimates of maximum justifiable expenditure have been compared with an estimate of the cost of non-surgical transfer. Embryo transfer should be used in elite beef herds to increase selection intensity, particularly if bulls from such herds can be used for artificial insemination. Other commercial applications will not be economically justifiable until the cost of transfer has fallen by 50 to 80 per cent.

Animals↗

Cyclical changes in the pituitary gonadotrophs (GTH cells) in relation to the testicular cycle in Puntius sarana (Hamilton).

The pituitary gland of Puntius sarana is of the leptobasic type and has three subdivisions--a proximal pars distalis (PPD), a rostral pars distalis (RPD) and a pars intermedia (PI) arranged vertically one below the other. The gonadotrophs (GTH cells) of the PPD were identified by their PAS, ATh, AF, aniline blue and alcian blue positivity and by their immunoreactivity to ovine anti-LH beta-subunit, anti-salmon GTH and anti-silver carp GTH. The testes of Puntius sarana have been classified as the "unrestricted type". The morphological, histological and histometric findings indicate that the testing passes through four distinct stages--preparatory, fully mature, spawning and spent. Spermatogenesis begins in January and continues up to July. The spawning season lasts from late July until October. Cyclical changes in the gonadotrophs correlate well with changes in the testes. The histometric data show that the GTH cells attain maximum values (size and number) during the breeding season. The peak values for testicular weight, lobule diameter, the gonadosomatic index (GSI) and the sperm concentration in the testes, and the number, size and amount of secretory material in the cytoplasm of the gonadotrophs, coincide with the time of maximum environmental temperature and the longest days.

Animals↗

Endocrine and testicular changes in a short-day seasonally breeding bird, the emu (Dromaius novaehollandiae), in southwestern Australia.

Seasonal changes in testicular morphology and blood plasma concentrations of LH, testosterone, and prolactin are described for captive male emus in southwestern Australia. Testicular mass and testicular testosterone did not differ between the non-breeding (spring-summer) and the breeding (autumn-winter) seasons. Nevertheless, the testes obtained in the breeding season (May and August) were nearly two fold greater in mass than those collected in the non-breeding season (October and February). The highest testicular concentrations of testosterone were observed in February and lowest in October, while the values during the breeding season were intermediate. The patterns of histological changes in the testes also indicate that emus breed over the autumn-winter months. Tubule diameter was larger in the breeding season than in the non-breeding season, whereas the relative volume of the interstitium was larger in the non-breeding and smaller in the breeding season. Moreover, during the autumn and winter months, plasma LH and testosterone concentrations were high. Outside this period, in spring and summer, the concentrations of these hormones were low. Prolactin concentrations rose around the winter solstice, after the initial increases in plasma LH and testosterone. The end of the breeding season, in early spring, was marked by a gradual decrease in plasma LH concentrations but a rapid fall in testosterone concentrations. Prolactin concentrations continued to increase and peaked near the spring equinox, several weeks after the breeding season ended, and then decreased to reach baseline values by mid-summer. These testicular and endocrine changes are consistent with observations that the emu is a short-day breeder in southwestern Australia. Reproductive activity in the male begins soon after the summer solstice, well in advance of the development of suitable breeding conditions, and is then terminated in spring before food resources become limited by the onset of the dry season.

Animals↗

Genetic variation of Polish endangered Biłgoraj horses and two common horse breeds in microsatellite loci.

Genetic variation of endangered Biłgoraj horses and two common Polish horse breeds was compared with the use of 12 microsatellite loci (AHT4, AHT5, ASB2, HMS2, HMS3, HMS6, HMS7, HTG4, HTG6, HTG7, HTG10, VHL20). Lower allelic diversity was detected in all investigated populations in comparison to other studies. Large differences in the frequencies of microsatellite alleles between Biłgoraj horses and two other horse breeds were discovered. In all polymorphic loci all investigated breeds were in the Hardy-Weinberg equilibrium. Mean Fis values and the results of a test for the presence of a recent bottleneck were non-significant in all studied populations. Comparable values of observed and expected gene diversity indicate no substantial loss of genetic variation in the Biłgoraj population and two other breeds. The lowest variability observed in the investigated group of Thoroughbred horses was confirmed. About 10% of genetic variation are explained by differences between breeds. Values of pairwise Fst and two measures of genetic distance demonstrated that Biłgoraj horses are distantly related to both common horse breeds.

Animals↗

Simulated influence of postweaning production system on performance of different biological types of cattle: I. Estimation of model parameters.

Breed parameters for a computer model that simulated differences in the composition of empty-body gain of beef cattle, resulting from differences in postweaning level of nutrition that are not associated with empty BW, were estimated for 17 biological types of cattle (steers from F1 crosses of 16 sire breeds [Hereford, Angus, Jersey, South Devon, Limousin, Simmental, Charolais, Red Poll, Brown Swiss, Gelbvieh, Maine Anjou, Chianina, Brahman, Sahiwal, Pinzgauer, and Tarentaise] mated to Hereford and Angus dams). One value for the maximum fractional growth rate of fat-free matter (KMAX) was estimated and used across all breed types. Mature fat-free matter (FFMmat) was estimated from data on mature cows for each of the 17 breed types. Breed type values for a fattening parameter (THETA) were estimated from growth and composition data at slaughter on steers of the 17 breed types, using the previously estimated constant KMAX and breed values for FFMmat. For each breed type, THETA values were unique for given values of KMAX, FFMmat, and composition at slaughter. The results showed that THETA was most sensitive to KMAX and had similar sensitivity to FFMmat and composition at slaughter. Values for THETA were most sensitive for breed types with large THETA values (Chianina, Charolais, and Limousin crossbred steers) and least sensitive for breed types with small THETA values (purebred Angus, crossbred Jersey, and Red Poll steers).(ABSTRACT TRUNCATED AT 250 WORDS)

Aging↗

[Ecological surveillance on breeding ground for Oncomelania hupensis snails in the areas prevalent with islet-type schistosomiasis using remote sensing technology].

OBJECTIVE: To probe rational indices suitable for ecological surveillance on breeding ground for Oncomelania hupensis snails in areas prevalent with islet-type schisitosomiasis using remote sensing technology. METHODS: Three adjacent islets, prevalent with islet-type schistosomiasis, along the Yangtze River within the boundaries of Dongzhi County, Anhui Province were selected as study field for remote sensing analysis. Multi-spectral data were composed and non-supervisedly classified in computer with Idisi software for remote sensing analysis. Values of the normalized difference vegetation index (NDVI), green vegetation index (GVI), bright index (BI), which reflect the greenness and brightness of landscape, were also calculated. Finally, all the results were comprehensively analyzed, combined with data from the field investigation. RESULTS: NDVI, GVI and BI could depict characteristics of the landscape quantitatively. Values of NDVI, BI and GVI were varied in different types of landscapes, and 95% confidence interval of these values suitable for breeding of snails was 0.0522 approximately 0.3566, 2.4162 approximately 28.2672 and 29.3404 approximately 40.3135, respectively. Classification of NDVI showed that type 5 anf type 6 were main breeding ground for snails, and type 4 with values of NDVI from 0 to 0.1 was potential areas for snail propagation. Classification of GVI showed that types 5, 6 and 7 were main breeding ground for snails, and also type 4 with values of GVI from 2 to 10 was potential areas for snail propagation. Both NDVI and GVI showed type 2 and type 3 were temporarily not suitable for snail breeding. CONCLUSION: Index figures of NDVI and GVI re-formed by reasonable classification could reflect not only breeding ground for snails and range of the areas for snail propagation in islets, but also their evolving rules, i.e., status of new marshland formation and vegetation growth.

Animals↗

Plasmatic profiles of pregnancy-associated glycoprotein and progesterone levels during gestation in Churra and Merino sheep.

This study was carried out to determine ovine pregnancy-associated glycoprotein (oPAG) and progesterone (P4) levels in the serum of Churra and Merino ewes throughout gestation and the first month post partum. The oPAG levels were determined with an heterologus RIA using bovine PAG as standard and tracer and rabbit antiserum against oPAG, sensitivity was 4.0 ng/ml. The P4 levels were measured with a radioimmunological procedure, including a specific extraction step with petroleum ether (bp 60-80 degrees C) with a sensitivity of less than 0.1 ng/ml. There were no differences (P<0.10) in the oPAG profile between breeds from Weeks 1 to 18. From Week 18 to lambing, oPAG concentrations increased rapidly in Churra ewes (on average, from 250 to 650 ng/ml) while remaining relatively constant in the Merino ewes (around 250 ng/ml). No significant differences (P>0.05) were observed for mean weekly P4 levels between the 2 breeds. In both breeds, P4 increased throughout the whole length of gestation, with the highest level measured at Weeks 19-20, then declined 2 wk before parturition. No correlation was found between P4 and oPAG concentrations during gestation in either of the breeds. After lambing, oPAG and P4 levels decreased rapidly in 4 wk to basal values. In both breeds the oPAG concentrations at Weeks 19, 20 and 21 of gestation in ewes carrying male fetuses were higher than in those carrying female fetuses. From the results, we conclude that the breed and sex of the fetus could influence the production of oPAG.

Journal Article↗

Breed distribution of dogs with diabetes mellitus admitted to a tertiary care facility.

OBJECTIVE: To determine which dog breeds are at low and high risk for developing diabetes mellitus (DM). DESIGN: Cohort study. ANIMALS: Hospital population of 221 dogs with DM and 42,882 dogs without DM during 5.5 years. PROCEDURE: 165 breeds (including a mixed-breed category) were represented in the hospital population. Breed-specific expected numbers of dogs with DM were calculated by multiplying the proportion of all dogs admitted to the hospital that were determined to have DM during the study period by the breed-specific totals during the study period. Breeds or breed groups evaluated in the analysis (n = 20) were restricted to those that had a combined observed and expected count > 5 to document breeds at low and high risk for developing DM. Proportionate changes in the risk of developing DM by breed were calculated and presented using exact odds ratios, 95% confidence intervals, and P values. Mixed-breed dogs were chosen as the reference breed. RESULTS: Samoyeds, Miniature Schnauzers, Miniature Poodles, Pugs, and Toy Poodles were at high risk for developing DM. Dog breeds found to be at low risk for developing DM were German Shepherd Dog, Golden Retriever, and American Pit Bull Terrier. CONCLUSION AND CLINICAL RELEVANCE: The finding that certain dog breeds are at low or high risk for developing DM suggests that some genetic defects may predispose dogs to development of DM, whereas other genetic factors may protect dogs from development of DM.

Animals↗

Differences in whole-body protein turnover between Iberian and Landrace pigs fed adequate or lysine-deficient diets.

The capacity for protein deposition in Iberian pigs is lower than in modern (e.g., Landrace) pig breeds, and the reasons for this remain unknown. The hypothesis tested in this work is that under similar nutritional and physiological conditions, whole-body protein turnover as well as the protein synthesis to protein deposition ratio differs between Iberian and Landrace breeds, resulting in dissimilar protein deposition rates. As a main objective, these variables were compared at different protein and Lys intakes in growing gilts. The study examined the effect of Lys deficiency because this is the prevalent condition during the fattening period of the Iberian pig in the Mediterranean forest, where the main feed source is oak acorn, which provides approximately one-third of the available Lys present in an ideal protein. Three diets were tested within each breed: 2 diets with an optimal essential AA pattern, containing 12 or 16% CP as-fed, or a Lys-deficient diet (35% of the recommended Lys content). This diet was supplied at 12% CP for the Iberian and 16% CP for the Landrace pigs, respectively. The contrasts made were breed x dietary protein concentration and breed x AA pattern (adequate vs. inadequate Lys content). Cumulative urinary (15)N excretion over 60 h after receiving an oral dose of [(15)N]-glycine was used to calculate N flux. Mean BW for Landrace and Iberian pigs were 25.8 +/- 0.55 kg and 30.8 +/- 0.74 kg, respectively. Protein deposition (g of N/(kg(0.75).d) was lower in the Iberian than in the Landrace gilts (4 to 16%; P = 0.002) and increased with dietary protein content. In contrast, protein synthesis and degradation [g of N/(kg(0.75).d)] were greater for the Landrace breed (16 to 18 and 23%, respectively, for the 2 dietary protein contents studied; P < 0.05), but no breed differences were detected in fractional protein synthesis and degradation rates. The ratio of protein synthesis:protein deposition (S/PD) did not change with dietary protein concentration or breed and achieved a mean value of 5.4. Irrespective of breed, Lys deficiency had a strong negative effect on N balance (P < 0.001) and increased the ratio of S/PD (P = 0.012). The greater rates of protein deposition, synthesis, and degradation in Landrace pigs than in Iberian pigs fed optimal AA-pattern diets were then attributed to differences in body protein mass. Consequently, these results validate the hypothesis of unequal synthesis and degradation, but not of unequal S/PD, between breeds.

Animal Feed↗

Serum biochemistry of healthy Yucatan miniature pigs.

Sera collected from 24 (12 boars and 12 gilts) healthy, fasted 28-43 week-old Yucatan miniature pigs were analyzed for 21 clinical chemistry parameters. The mean, standard deviation, median, and observed range for each parameter are presented as reference values for the normal blood chemistry for this breed. Comparison with published values reveals that the Yucatan miniature pig has a blood chemistry profile comparable to that of domestic pigs and other breeds of miniature swine.

Animals↗

Use of a sperm quality analyser on semen of turkey breeders to monitor storage time effects and age-related changes during a reproductive cycle.

1. A relatively new instrument known as a Sperm Quality Analyzer (SQA) offers a rapid assessment of sperm quality and quantity by providing a sperm quality index (SQI). The SQA measures the intensity of sperm activity and motile concentration by determining the number and amplitude of sperm movements per second in a capillary tube as detected through light beam interference. 2. The objectives of the current study were to determine if the SQA could accurately reflect changes in semen quality that occur with prolonged storage of semen and to determine the variation and change in SQI values among individual breeding male turkeys during their semen production cycle. 3. The effect of storage time on SQI values was evaluated by diluting semen with extender and placing the semen on an oscillating shaker at 4 degrees C for 8 h. The SQI values and sperm viability, expressed as % dead sperm, were recorded hourly. The SQI readings declined linearly with increased storage time while % dead sperm increased linearly with increased semen storage. 4. Semen from 220 individual males was analysed monthly for 9 months. Semen diluted 50-fold with saline had lower SQI values during pre- and post-peak phases of production (months 1, 7, 8, and 9 as compared with months 2 to 6 of semen production). The highest SQI values occurred during months 2 to 6. The largest variation in SQI values occurred during months 1 (CV = 26%) and 9 (CV = 31%) with a CV that averaged 16% for the remaining months. 5. Correlation analysis of SQI values for each bird averaged over 9 months with individual male SQIs for each month showed monthly correlation coefficients that ranged from 0.22 to 0.63. 6. These results indicate that the SQA accurately assessed the decline in sperm quality that occurs with prolonged storage of turkey semen and reflected age-related changes in semen quality and quantity that occurred during a semen production cycle of turkey breeders. In addition, the semen quality rank of some turkey breeders in a population changed with age.

Aging↗

[Values of acido-basic balance in the blood of the beagle breed dog in comparison with the German shepherd dog].

The values of acidobasic balance were examined in 101 dogs of the Beagle breed and in 20 German Sheep-Dogs. The Beagle dogs were divided into five age groups. A difference was found between the values obtained in the group of the youngest dogs and those in older age groups. In comparison with Beagle dogs, the values were higher in German Sheep-Dogs (pH, base excess). A figure is attached representing the deviations of the values of acidobasic balance of blood in the two breeds.

Acid-Base Equilibrium↗

Additive and heterosis effects on preweaning traits, maternal ability and reproduction from crossing of the Angus and brown Swiss breeds in Florida.

Angus (A), Brown Swiss (S) and A X S reciprocal F1 (AS) dams were mated to A, S and AS (also reciprocal F1) sires resulting in nine breed groups of progeny with varying proportions of Angus and Brown Swiss breeding. Breed group of dam and of sire significantly influenced birth weight, preweaning daily gain, weaning weight, 205-d weight, condition score and frame size. The means for birth weight and weaning weight were 33 and 213 kg, respectively. Brown Swiss bulls sired calves with the heaviest birth and weaning weights. Calves produced by S dams likewise were heavier at birth and weaning. Pregnancy rates were influenced significantly by year, age and breed of dam and averaged 79, 95 and 92% for S, AS and A cows, respectively. Survival rate averaged 97% and was not influenced significantly by any of the effects examined. Because survival rates were similar for all breed groups, the results for weaning rate paralleled those for pregnancy rate. Genetic influences on preweaning growth traits and survival rate were partitioned into additive breed differences (B) and heterosis (H) effects for direct (d) and maternal (m) components. Pregnancy and weaning rates were examined using similar analyses except that genotype of service sire of dam replaced that of the offspring for the direct additive breed and direct heterosis components. The Bd values indicated that the Angus breed was inferior (P less than .01) to the Brown Swiss breed for all preweaning growth traits except for condition score, in which the Angus breed surpassed (P less than .01) the Brown Swiss. The Bm values also showed an advantage for the Brown Swiss breed for all preweaning growth traits. The additive maternal effect (the genotype of the females exposed), Bm, was important for pregnancy rate and weaning rate (P less than .001 and P less than .05) but not for survival rate (P greater than .10). The direct additive breed effect was not important for any reproductive trait. Direct heterosis did not affect any of the preweaning or reproduction traits; however, maternal heterosis (Hm) significantly affected all traits except birth weight, frame score and survival rate. The Hm estimates were 12.0 and 8.4 kg for weaning weight and 205-d weight, respectively. The Hm estimates for pregnancy rate, survival rate and weaning rate were 10, 2 and 13%, respectively.

Animals↗

Iberian origins of New World horse breeds.

Fossil records, archaeological proofs, and historical documents report that horses persisted continuously in the Iberian Peninsula since the Pleistocene and were taken to the American continent (New World) in the 15th century. To investigate the variation within the mitochondrial DNA (mtDNA) control region of Iberian and New World horse breeds, to analyze their relationships, and to test the historical origin of New World horses, a total of 153 samples, representing 30 Iberian and New World breeds, were analyzed by sequencing mtDNA control region fragments. Fifty-four haplotypes were found and assigned to seven haplogroups. Reduced levels of variation found for the Menorquina, Sorraia, and Sulphur Mustang breeds are consistent with experienced bottlenecks or limited number of founders. For all diversity indices, Iberian breeds showed higher diversity values than South American and North American breeds. Although, the results show that the Iberian and New World breeds stem from multiple origins, we present a set of genetic data revealing a high frequency of Iberian haplotypes in New World breeds, which is consistent with historical documentation.

Americas↗

Effects of percentage Brahman and Angus breeding, age-season of feeding and slaughter end point on meat palatability and muscle characteristics.

Steers (n = 125) of known percentage Angus (A) and Brahman (B) breeding (A = 31, 3/4A:1/4B = 32, 1/2A:1/2B = 31, 1/4A:3/4B = 31) were slaughtered after being fed as calves during the cool period of the year or fed as yearlings during the warm period of the year. Steers were slaughtered at equivalent outside fat thickness as monitored visually and with real-time ultrasound. Warner-Bratzler shear (WBS) force increased and sensory panel tenderness decreased as percentage Brahman increased. Loin muscle characteristics indicated that differences in tenderness between breed groups were not attributed to cold shortening effects or differences in amount or integrity of connective tissue. Fragmentation values suggested that breed group tenderness differences probably resulted from differences in the muscle fiber component. A 10-d postmortem aging study revealed a differential breed group response to postmortem aging, suggesting that breed groups differed in amount and(or) activity of naturally occurring proteolytic enzymes in muscle tissue.

Age Factors↗

Testosterone and survival: a cost of aggressiveness?

Free-living male brown-headed cowbirds were captured during the breeding season, implanted with testosterone-filled Silastic tubing, and released. Radioimmunoassay verified that the implants maintained circulating plasma testosterone values at maximal breeding season levels well beyond the normal time of decline. Survival to the following year of these implanted males was compared with survival of unimplanted birds captured in other years, and also with survival of males given empty implants. Androgen-implanted male cowbirds exhibited significantly reduced survival to the following year compared with either of the control groups, and also exhibited severe injuries not seen in other years. It is suggested that the increased risks associated with prolonged high testosterone levels act as a selective force to maintain reduced androgen levels except during the period of aggressive intrasexual interactions that characterizes the reproductive season. Further, it is proposed that the nature and importance of the different risks vary with a species' mating system.

Aggression↗