SEARCH · PubMed Health

Results for “Condensates”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 613 records · Page 34Linked to original sources

PubMed1999

Orbital Interactions and Solvent Effects Determining the Stability of Condensed Cyclopentadienides in Solution.

The structure and the stability of various cyclopentadienides, which involve 6pi, 10pi, 14pi, and 22pi electrons, are investigated from computations at various levels of theory as well as from orbital interaction analyses. The reason that some of the cyclopentadienides are stabilized and others are destabilized by the introduction of aromatic rings is discussed in terms of absolute hardness and orbital interaction. Cyclopentadienide, a special 6pi-electron system, has the largest value of absolute hardness among the condensed cyclopentadienides investigated; thus this carbanion resists both oxidation and reduction most strongly. The absolute hardness decreases when aromatic rings are introduced to cyclopentadienide to form condensed cyclopentadienides, depending on the way they are connected. Computed values of the ionization potential and oxidation potentials measured in solution have a linear correlation within isomers of the same size, but are not in agreement for different sets of isomers. Solvent effects on the ionization potential are assessed by performing self-consistent reaction field calculations, the results being in excellent agreement with experiments. It is demonstrated that the solvent effects are significant in small cyclopentadienides of 6pi- and 10pi-electron systems, compared to larger ones and that addition of condensed aromatic rings intrinsically stabilizes the formed condensed cyclopentadienides with respect to ionization potential.

Journal Article↗

PubMed2005

Comparison among four different ways to condense the Fukui function.

Four different ways to condense the Fukui function are compared. Three of them perform a numerical integration over different basins to define the condensed Fukui function, and the other one is the most traditional Fukui function using Mulliken population analysis. The basins are chosen to be the basins of the electron density (AIM), the basins of the electron localization function (ELF), and the basins of the Fukui function itself. The use of the last two basins is new and presented for the first time here. It is found that the last three methods yield results which are stable against a change in the basis set. The condensed Fukui function using the basins of the ELF is not able to give information on the reactivity of an acceptor molecule. In general, the condensed Fukui function using the basins of the density or the basins of the Fukui function describe the reactivity trends well. The latter is preferred, because it only contains information about the Fukui function itself and it gives the right information for donor as well as acceptor centers.

Journal Article↗

PubMed2005

Condensed-phase effects on the structural properties of C6H5CN-BF3 and (CH3)3CCN-BF3: IR spectra, crystallography, and computations.

Condensed-phase effects on the structure and bonding of C(6)H(5)CN-BF(3) and (CH(3))(3)CCN-BF(3) are illustrated by a variety of results, and these are compared to analogous data for the closely related complex CH(3)CN-BF(3). For the most part, the structural properties of C(6)H(5)CN-BF(3) and (CH(3))(3)CCN-BF(3) are quite similar, not only in the gas phase but also in the solid state and in argon matrices. However, the structures do change significantly from medium to medium, and these changes are reflected in the data presented below. Specifically, the measured crystallographic structure of C(6)H(5)CN-BF(3) (s) has a B-N distance that is 0.17 A shorter than that in the equilibrium gas-phase structure obtained via B3LYP calculations. Notable differences between calculated gas-phase frequencies and measured solid-state frequencies for both C(6)H(5)CN-BF(3) and (CH(3))(3)CCN-BF(3) were also observed, and in the case of (CH(3))(3)CCN-BF(3), these data implicate a comparable difference between solid-state and gas-phase structure, even in the absence of crystallographic results. Frequencies measured in argon matrices were found to be quite similar for both complexes and also very near those measured previously for CH(3)CN-BF(3), suggesting that all three complexes adopt similar structures in solid argon. For C(6)H(5)CN-BF(3) and (CH(3))(3)CCN-BF(3), matrix IR frequencies differ only slightly from the computed gas-phase values, but do suggest a slight compression of the B-N bond. Ultimately, it appears that the varying degree to which these systems respond to condensed phases stems from subtle differences in the gas-phase species, which are highlighted through an examination of B-N distance potentials from B3LYP calculations. The larger organic substituents appear to stabilize the potential near 1.8 A, so that the structures are more localized in that region prior to any condensed-phase interactions. As a result, the condensed-phase effects on the structural properties of C(6)H(5)CN-BF(3) and (CH(3))(3)CCN-BF(3) are much less pronounced than those for CH(3)CN-BF(3).

Journal Article↗

PubMed2006

Effects of condensing agent and nuclease on the extent of ejection from phage lambda.

We have recently demonstrated, that DNA ejection from bacteriophage lambda can be partially or completely suppressed in vitro by external osmotic pressure. This suggests that DNA ejection from phage is driven by an internal mechanical force consisting of DNA bending and DNA-DNA electrostatic repulsion energies. In the present work we investigate the extent to which DNA ejection is incomplete at zero osmotic external pressure when phage is opened with its receptor in vitro. The DNA fragment remaining in the capsid and the tail that is no longer bent or compressed -and hence for which there is no internal driving force for ejection- is shown not to be ejected. We also demonstrate that DNA can be "pulled" out from the capsid by DNase I acting as a DNA binding protein or spermine acting as a DNA condensing agent. In particular, cryo electron microscopy and gel electrophoresis experiments show the following: (i) DNA ejection from bacteriophage lambda incubated in vitro with its receptor is incomplete at zero external osmotic force, with several persistence lengths of DNA remaining inside the phage capsid, if no nuclease (DNase I) or DNA condensing agent (spermine) is present in the host solution; (ii) in the presence of both DNase I and spermine in the host solution, 60% (approximately 29 kbp) of wild-type lambda DNA (48.5 kbp) remains unejected inside the phage capsid, in the form of an unconstrained toroidal condensate; (iii) with DNase I added, but no spermine, the ejection is complete; (iv) with spermine, but without DNase I added, all the DNA is again ejected, and organized as a toroidal condensate outside.

Bacteriophage lambda↗

PubMed2006

Condensation on ultrahydrophobic surfaces and its effect on droplet mobility: ultrahydrophobic surfaces are not always water repellant.

The condensation of water was studied on topography-based ultrahydrophobic surfaces containing hydrophobized silicon pillars. Optical microscopy showed that water nucleated and grew both on top of and between the pillars. As condensation progressed, water between the pillars became unstable and was forced upward to the surface. Macroscopic water droplets on top of the pillars coalesced with condensed water that remained between the pillars, pinning the droplets at their three-phase contact line. Dynamic contact angle measurements on ultrahydrophobic surfaces wet with condensation revealed a dramatic increase in hysteresis compared to that on dry surfaces, leading to a corresponding decrease in water drop mobility.

Journal Article↗

PubMed2006

Dropwise condensation: experiments and simulations of nucleation and growth of water drops in a cooling system.

Dropwise condensation of water vapor from a naturally cooling, hot water reservoir onto a hydrophobic polymer film and a silanized glass slide was studied by direct observation and simulations. The observed drop growth kinetics suggests that smallest drops grow principally by the diffusion of water adsorbed on the substrate to the drop perimeter, while drops larger than about 50 microm in diameter grow principally by direct deposition from the vapor onto the drop surface. Drop coalescence plays a critical role in determining the drop-size distribution and stimulates the nucleation of new, small drops on the substrates. Simulations of drop growth incorporating these growth mechanisms provide a good description of the observed drop-size distribution. Because of the large role played by coalescence, details of individual drop growth make little difference to the final drop-size distribution. The rate of condensation per unit substrate area is especially high for the smallest drops and may help account for the high heat transfer rates associated with dropwise condensation relative to filmwise condensation in heat exchange applications.

Cold Temperature↗

PubMed2004

Single molecule fluorescence imaging and its application to the study of DNA condensation.

Single molecule fluorescence imaging incorporated with optical tweezers and a laminar flow cell has been used to monitor the kinetic process of DNA condensation induced by spermidine. It was found that at least two steps were involved in the condensation process of the hydrodynamically-stretched linear DNA; a lag period followed by a rapid collapse of DNA. The lag time increased with the flow speed and the collapse time remained short within the range of the flow speed studied. The effect of salt concentration on the condensation process was examined, and the results suggest that the longer lag time observed in the higher salt buffer probably results from the displacement of bound cations and rearrangement of spermidine on the DNA. The flow-speed dependence of the lag time suggests that a nucleation event at the free end of the DNA, i.e. formation of a loop, may play a vital role in the kinetic process of condensation.

DNA↗

PubMed2000

Measurement of inflammatory markers in the breath condensate of children with cystic fibrosis.

Identifying noninvasive markers of pulmonary inflammation would be useful in assessing new therapies in children. Breath condensate is a simple and potentially acceptable sample medium even in small children. The technique has previously been used in adults, but not children with cystic fibrosis. The technique was assessed in 36 children with cystic fibrosis (mean age 10.4 yrs) and 17 control subjects, analysing samples for nitrite, interleukin(IL)-8 and salivary and nasal contamination. Correlations were made between levels of the inflammatory markers and forced expiratory volume in one second/forced vital capacity, chest radiograph score and use of inhaled steroids. On samples without significant contamination (<10 u x L(-1) amylase) nitrite was detected in 93% of samples at a median concentration of 3.0 microM compared with 50% of control samples at a median of 0.5 microM. Condensate amylase levels did not correlate with the nitrite value obtained (r=0.31). IL-8 was detected in 33% of CF samples. Breath condensate is an acceptable method of sample collection in children. Nitrite was raised in breath condensate from patients with cystic fibrosis when compared with control subjects.

Breath Tests↗

PubMedDate not supplied

The condensing vacuole of exocrine cells is more acidic than the mature secretory vesicle.

A number of intracellular, membrane-bound compartments in both the endocytic and exocytic pathways of eukaryotic cells have an acidic internal pH. In endocrine cells, the mature secretory vesicle has an acidic pH; secretory vesicles isolated from exocrine cells, however, appear to have a neutral pH. Recently we have used a newly developed immunocytochemical technique to map low-pH compartments in insulin-secreting islet cells with the electron microscope and find that during the maturation of the secretory vesicle there is a progressive acidification of these vesicles that begins as soon as the trans Golgi condensing vacuoles form. Now we have used this technique to examine two exocrine cells: the pancreatic acinar cell and the parotid serous cell. In both cell types, the trans Golgi condensing vacuoles are acidic and accumulate the low-pH probe to the same extent as condensing vacuoles of insulin-secreting islet cells. Unlike insulin-secreting cells, however, maturation of the granules is accompanied by a return of luminal pH to near neutrality. Therefore, although the pH of storage granules in exocrine and endocrine cells is different, the pH of the condensing vacuoles in both cells is acidic.

Amylases↗

PubMed2002

Cnd2 has dual roles in mitotic condensation and interphase.

Chromosome condensation requires condensin, which comprises five subunits. Two of these subunits--both being structural maintenance of chromosome (SMC) proteins-are coiled-coils with globular terminal domains that interact with ATP and DNA. The remaining three, non-SMC subunits also have essential, albeit undefined, roles in condensation. Here we report that Cnd2 (ref. 6), a non-SMC subunit of fission yeast similar to Drosophila Barren and the budding yeast protein Brn1 (refs 8, 9), is required for both interphase and mitotic condensation. In cnd2-1 mutants, ultraviolet-induced DNA damage is not repaired, and cells arrested by hydroxyurea do not recover. A definitive defect of interphase is abolishment of Cds1 (a checkpoint kinase) activation in the presence of hydroxyurea in both cnd2-1 mutant cells and in cells where other condensin subunits have been genetically disrupted. In the absence of hydroxyurea, a G2 checkpoint delay occurred in cnd2-1 mutants in a manner dependent on Cds1 and ATM-like Rad3, but not Chk1 (refs 10-13), before the mitotic condensation defect. Furthermore, cnd2-1 was synthetic-lethal with mutations of excision repair, RecQ helicase and DNA replication enzymes. These interphase and mitotic defects provide insight into the mechanistic role of non-SMC subunits that interact with the globular SMC domains in the heteropentameric holocomplex.

Cell Cycle Proteins↗

PubMed1977

Fractionation of mouse skin carcinogens in cagarette smoke condensate.

The results of a series of mouse-skin paintings are given for fractions prepared by two schemes designed to concentrate the polycyclic aromatic hydrocarbons (PAH) and their heterocyclic analogues (HETC) present in cigarette smoke condensate into single fractions. It is demonstrated that, for each group, a single index of tumour response, the "Weibull risk parameter" (WRP), can be calculated which, considered in conjunction with two other parameters common to all the groups, adequately describes the pattern of tumour incidence in that group. These indices can be used to calculate for each fraction a further statistic, the "tumorigenic ratio" (TR), which conveniently measures the activity of the fraction relative to whole-smoke condensate on a weight-for-weight basis. From the analyses it is shown that the separation processes can successfully concentrate all types of mouse-skin carcinogenic material, irrespective of the type of condensate used, and that a combination of processes prepares an active concentrate representing 2% by weight of the original condensate.

Animals↗

PubMed2002

Collapse and revival of the matter wave field of a Bose-Einstein condensate.

A Bose-Einstein condensate represents the most 'classical' form of a matter wave, just as an optical laser emits the most classical form of an electromagnetic wave. Nevertheless, the matter wave field has a quantized structure owing to the granularity of the discrete underlying atoms. Although such a field is usually assumed to be intrinsically stable (apart from incoherent loss processes), this is no longer true when the condensate is in a coherent superposition of different atom number states. For example, in a Bose-Einstein condensate confined by a three-dimensional optical lattice, each potential well can be prepared in a coherent superposition of different atom number states, with constant relative phases between neighbouring lattice sites. It is then natural to ask how the individual matter wave fields and their relative phases evolve. Here we use such a set-up to investigate these questions experimentally, observing that the matter wave field of the Bose-Einstein condensate undergoes a periodic series of collapses and revivals; this behaviour is directly demonstrated in the dynamical evolution of the multiple matter wave interference pattern. We attribute the oscillations to the quantized structure of the matter wave field and the collisions between individual atoms.

Journal Article↗

PubMed2003

Bose-Einstein condensation of the triplet states in the magnetic insulator TlCuCl3.

Bose-Einstein condensation denotes the formation of a collective quantum ground state of identical particles with integer spin or intrinsic angular momentum. In magnetic insulators, the magnetic properties are due to the unpaired shell electrons that have half-integer spin. However, in some such compounds (KCuCl3 and TlCuCl3), two Cu2+ ions are antiferromagnetically coupled to form a dimer in a crystalline network: the dimer ground state is a spin singlet (total spin zero), separated by an energy gap from the excited triplet state (total spin one). In these dimer compounds, Bose-Einstein condensation becomes theoretically possible. At a critical external magnetic field, the energy of one of the Zeeman split triplet components (a type of boson) intersects the ground-state singlet, resulting in long-range magnetic order; this transition represents a quantum critical point at which Bose-Einstein condensation occurs. Here we report an experimental investigation of the excitation spectrum in such a field-induced magnetically ordered state, using inelastic neutron scattering measurements of TlCuCl3 single crystals. We verify unambiguously the theoretically predicted gapless Goldstone mode characteristic of the Bose-Einstein condensation of the triplet states.

Journal Article↗

PubMed2006

Spontaneous symmetry breaking in a quenched ferromagnetic spinor Bose-Einstein condensate.

A central goal in condensed matter and modern atomic physics is the exploration of quantum phases of matter--in particular, how the universal characteristics of zero-temperature quantum phase transitions differ from those established for thermal phase transitions at non-zero temperature. Compared to conventional condensed matter systems, atomic gases provide a unique opportunity to explore quantum dynamics far from equilibrium. For example, gaseous spinor Bose-Einstein condensates (whose atoms have non-zero internal angular momentum) are quantum fluids that simultaneously realize superfluidity and magnetism, both of which are associated with symmetry breaking. Here we explore spontaneous symmetry breaking in 87Rb spinor condensates, rapidly quenched across a quantum phase transition to a ferromagnetic state. We observe the formation of spin textures, ferromagnetic domains and domain walls, and demonstrate phase-sensitive in situ detection of spin vortices. The latter are topological defects resulting from the symmetry breaking, containing non-zero spin current but no net mass current.

Journal Article↗

PubMed2002

The structure of VibH represents nonribosomal peptide synthetase condensation, cyclization and epimerization domains.

Nonribosomal peptide synthetases (NRPSs) are large, multidomain enzymes that biosynthesize medically important natural products. We report the crystal structure of the free-standing NRPS condensation (C) domain VibH, which catalyzes amide bond formation in the synthesis of vibriobactin, a Vibrio cholerae siderophore. Despite low sequence identity, NRPS condensation enzymes are structurally related to chloramphenicol acetyltransferase (CAT) and dihydrolipoamide acyltransferases. However, although the latter enzymes are homotrimers, VibH is a monomeric pseudodimer. The VibH structure is representative of both NRPS condensation and epimerization domains, as well as the condensation-variant cyclization domains, which are all expected to be monomers. Surprisingly, despite favorable positioning in the active site, a universally conserved histidine important in CAT and in other C domains is not critical for general base catalysis in VibH.

Acyltransferases↗

PubMed1983

Inhibition of the condensing component of chicken liver fatty acid synthase by iodoacetamide and 5,5'-dithiobis-(2-nitrobenzoic acid).

Chicken liver fatty acid synthase is inhibited by the thiol-modifying reagents 5,5'-dithiobis-(2-nitrobenzoic acid) and iodoacetamide. Total inactivation of the activity for fatty acid synthesis requires the modification of about 8 of the nearly 50 freely accessible thiol groups per molecule. The differential binding of iodo[14C]acetamide to phenylmethylsulphonyl fluoride-modified enzyme in the absence and in the presence of excess acetyl-CoA shows complete modification of one cysteine-SH site of the condensing enzyme and partial modification of the pantetheine-SH site for a total of approx. 1.4 mol of iodoacetamide bound per mol of enzyme. The reaction of the enzyme with 5,5'-dithiobis-(2-nitrobenzoic acid) generates disulphide cross-links for each molecule of the reagent added, but 95% of these cross-links are intrasubunit. Both the iodoacetamide- and 5,5'-dithiobis-(2-nitrobenzoic acid)-modified species catalyse all the component partial reactions of fatty acid synthesis except the condensation reaction. The results obtained with iodoacetamide show that in the dimeric fatty acid synthase modification of one cysteine-SH condensing site and/or one pantetheine-SH site per dimer is sufficient to affect inhibition of condensing activity and the activity for fatty acid synthesis, and are in accord with a recently proposed model for the mechanism of action of animal fatty acid synthases [Kumar (1982) J. Theor. Biol. 95, 263-283].

Acetyl Coenzyme A↗

PubMed2004

Correlation of nitrites in breath condensates and lung function in asthmatic children.

We aimed to evaluate the value of exhaled breath condensates in monitoring airway inflammation in childhood asthma before and after high altitude climate therapy. Forty-eight asthmatic children on regular anti-asthma treatment with a normal FEV1 and positive skin prick test for house dust mites were recruited. All children had been referred to an alpine clinic for high altitude climate therapy, because of persistent asthmatic symptoms despite use of daily anti-inflammatory treatment. Subjects were assessed on their arrival and before departure from the alpine clinic. Spirometry, bronchial provocation tests and measurements of nitrites in breath condensates were performed. Median levels of nitrites were significantly higher before than after high altitude climate therapy (1.27 vs. 0.93 microm; p = 0.008). In addition, MEF50 improved significantly (p < 0.0005). There was a significant correlation between nitrites in breath condensates and MEF50 (r = -0.63, p < 0.0001), symptoms (r = 0.47, p = 0.0007) and airway hyper-reactivity (AHR) (r = -0.41, p = 0.004). In summary, we found a reduction in nitrites in breath condensates after a high altitude climate therapy. Significant correlations were found between nitrites and MEF50, AHR and symptoms. We conclude that the measurement of nitrites may be feasible to objectively assess airway inflammation in asthmatic children in order to detect ongoing inflammation in children with normal FEV1 but persistent symptoms.

Adolescent↗

PubMed2000

Window pane condensation and high indoor vapour contribution - markers of an unhealthy indoor climate?

OBJECTIVE: The aim of this study was to investigate whether window pane condensation and indoor vapour contribution >/= 3 g/m3 could be used as indicators of defective air change rate, high indoor humidity and high mite allergen concentration in mattress dust. METHODS: Actual ventilation rate, indoor temperature, air humidity (AIH/RH) and concentrations of mite allergen were measured in 59 houses and compared with received outdoor temperatures and air humidity. Indoor vapour contribution defined as the difference between the indoor and the outdoor vapour concentration was calculated. Sensitivity, specificity, predictive values and accuracy were calculated for window pane condensation and high vapour contribution (>/= 3 g/m3), as indicators of defective ventilation (< 0.5 ACH), high indoor humidity (>/= 7 g/kg and >/= 45% RH) and high mite allergen concentration in mattress dust (>/= 2 microg/g). RESULTS: All houses with high humidity and high mite allergen concentrations were positive for the two indicators (high sensitivity), but with a specificity of only 50% so that half of the houses with reported condensation and high vapour contribution turned out to be low pollution houses with adequate high ventilation levels. Both indicators had high negative predictive values and absence of the two indicators almost certainly (97-100%) excluded high indoor pollution with high humidity and high mite concentrations. Overall more than 70% of the dwellings were correctly classified by the two indicators. CONCLUSION: Absence of window pane condensation on double-glazed windows and low indoor vapour contribution (< 3 g/m3) during the winter are true markers of a dwelling without high indoor air humidity and without high mite allergen concentrations in mattress dust in houses in a cold temperate climate with subzero outdoor temperatures. The presence of the two indicators is associated with a 18-45% risk of high humidity and mite allergen concentrations so in this latter group further measurements are needed for correct classification.

Air Pollution, Indoor↗