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At least 613 records · Page 34Linked to original sources
PubMed1976

Immunofluorescent detection of adenovirus antigen in epidemic keratoconjunctivitis.

An immunofluorescent technique was used to demonstrate soluble adenoviral antigens in epithelial cells on conjunctival scrapings of patients with epidemic keratoconjunctivitis (EKC). Conjunctival scrapings for immunofluorescence and viral isolation studies were performed on 79 patients suspected of having EKC or other acute follicular or papillary conjunctivitides. Of 41 patients with clinical findings consistent with a diagnosis of EKC and three patients with pharyngoconjunctional fever, 43 were positive by immunofluorescence. All 39 patients with adenovirus isolation had positive immunofluorescence studies. The five remaining cases had other documented evidence of EKC. No false-positive responses were encountered in relation to the clinical diagnoses. The fluorescent staining was predominantly found in the cytoplasm. Speckled nuclear fluorescence was also noted. The cytoplasmic fluorescence is compatible with the adenovirus cellular replication cycle. The immunofluorescent technique was found to be a reliable, sensitive, specific, and rapid diagnostic technique for detection of group-reaction adenoviral antigens in conjunctival scrapings.

Adenoviridae↗
PubMed1991

[Sexually transmitted diseases and mother-infant transmission (epidemiology and prevention)].

Gonococcal ophthalmia neonatorum may be severe with a high risk of blindness; therefore prophylaxis is mandatory. Chlamydial ophthalmia is less severe and prevention remains less effective. Both microorganisms may be responsible of preterm labour and perinatal deaths. Congenital syphilis is an important public health problem in developing countries. Some recent papers underline the decrease of infestation when mothers may be screened during pregnancy (at least two times in initially seronegative mothers) and correctly treated by beta-lactamines.

Chlamydia trachomatis↗
PubMed1977

[Morphogenesis and features of the pathogenesis of adenovirus and chlamydial infections of the conjunctiva].

Morphogenesis of adenovirus (epidemic keratoconjunctivitis) and chlamydia (paratrachoma) infections was studied on the material consisting of scrapings of the conjunctiva and biopsy specimens taken from 410 patients, by histological, cytological, histo- and cytochemical electron-microscopic and immunohistochemical methods. The investigations conducted showed that the morphogenesis of adenovirus and chlamydia infection of the conjunctiva was underlaid by a complex of pathological disorders including changes in the epithelium, where the cytopathogenic effect of the pathogene was realized, and changes in the conjunctiva tissue proper, the latter being transformed in these infections into the organ of immunogenesis.

Adenoviridae Infections↗
PubMed1991

[Neonatal conjunctivitis after the abolition of compulsory Credé prophylaxis].

The prescript requiring application of silver nitrate eye drops (0.66% AgNO3) to the conjunctivae of the newborn within two hours after delivery was revoked in March 1985. The present study comprises a prospective investigation of the occurrence of microorganisms in specimens of eye secretion from neonates received during the period February-April 1986, and a review of findings of Neisseria species and B. catarrhalis in 3,485 specimens of ocular secretions and of C. trachomatis in 1,240 conjunctival scrapes received at the Neisseria Department 1986-1988. The numbers of cases of conjunctivitis neonatalis caused by the following microorganisms were: N. gonorrhoeae 8, N. cinerea 6, other Neisseria species 8, B. catarrhalis 49 and C. trachomatis 92. During the period 1984-1988, the total number of neonates with gonococcal conjunctivitis neonatalis was 18. The gonococcal infection was diagnosed within the first week of life in 50% of the cases but could occur as late as in the fourth week of life. Similarly, infection with B. catarrhalis was most common in neonates less than one week old (49%), whereas chlamydial infection was most common in the second week of life (39%). It is concluded that the eyes of neonates should be carefully observed for at least four weeks and that microbiological examinations for gonococci and chlamydia are indicated if signs of infection appear.

Conjunctivitis, Bacterial↗
PubMed1990

Culture, ELISA and immunofluorescence tests for the diagnosis of conjunctivitis caused by Chlamydia trachomatis in neonates and adults.

The relative value of culture, direct specimen antigen detection tests, i.e., enzyme-linked immunosorbent assay (ELISA) and immunofluorescence (IF) tests in the diagnosis of Chlamydia trachomatis infection was studied in 125 newborns and 121 adults with signs of conjunctivitis. Eye and nasopharyngeal samples were tested by culture using cycloheximide-treated or irradiated McCoy cells, ELISA (i.e., Chlamydiazyme, Abbott) and IF tests (i.e., Chlamyset, Orion and MicroTrak, Syva). Of the neonates, 70 (35 boys and 35 girls) and 54 (33 males and 21 females) of the adults were positive in one or both eyes in one or more tests: 191 (39%) in cultures, 173 (35%) in ELISA and 160 (33%), 176 (36%) in each of the IF tests. Using culture as standard reference, the sensitivities of ELISA and the IF tests were 88%, 81% and 87%, while the corresponding specificities were 99%, 98% and 97%, respectively. The predictive values for a negative test (PVN) were 93%, 89% and 92% and for a positive test (PVP) 98%, 96% and 94%. Of the 124 cases chlamydia-positive in the eyes, 67 (54%), 76 (61%), 64 (52%) and 70 (57%) were positive in nasopharyngeal samples in one or more of culture, ELISA and the two IF tests, respectively. The sensitivities of ELISA and the IF tests in nasopharyngeal samples were 87%, 78% and 81%, while the corresponding specificities were 90%, 93% and 91%, respectively. The predictive values for a negative (PVN) test were 95%, 92% and 93%, and for a positive test (PVP) 76%, 81%, and 77%. Nasopharyngeal swabs were more often positive in cases with 2 or more weeks' duration of symptoms than in those with shorter duration.

Adolescent↗
PubMed1986

Assessment of neonatal conjunctivitis with a direct immunofluorescent monoclonal antibody stain for Chlamydia.

Chlamydial conjunctivitis was diagnosed by direct immunofluorescent monoclonal antibody staining of conjunctival smears in 46 of 100 consecutive neonates with conjunctivitis. The remaining 54 infants had bacterial conjunctivitis most commonly caused by Staphylococcus, Streptococcus, and Haemophilus species. A comparison of the direct test with chlamydial cultures demonstrated a sensitivity of 100% and a specificity of 94%, whereas Giemsa stain had a sensitivity of 42% and a specificity of 98%. Nineteen percent of infants treated with oral erythromycin for chlamydial infections in accordance with Centers for Disease Control guidelines had clinical and laboratory evidence of persistent chlamydial conjunctivitis. We conclude that Chlamydia trachomatis is a major cause of neonatal conjunctivitis that can be effectively and rapidly diagnosed by direct immunofluorescent monoclonal antibody staining of conjunctival smears. Further evaluation of treatment regimens appears to be warranted.

Antibodies, Bacterial↗
PubMedDate not supplied

[Prevalence of Chlamydia trachomatis infection in infants & children].

To understand the prevalence of Chlamydial infection in children in the central area of Taiwan, 220 cases were surveyed in the hospital nursery and pediatric ward from September 1985 to June 1986. In these 10 months, using the enzyme immunoassay (EIA) method, the patients were divided into two groups. Group A included 98 newborn cases, in the nursery, whose conjunctival secretions were collected within two hours after birth. All cases were then treated with erythromycin ophthalmic ointment. Group B included 122 cases, from the pediatric ward, who suffered from symptoms of respiratory tract disease; aspirated secretions from the trachea-were studied. The antigen positive rate in Group A was 12.2% (12 cases), male to female ratio was 1:5. However, in Group B the antigen positive ratio was 26.2% (32 cases), with male to female ratio of approximating 5:3. Children under two years old had the highest incidence of infection, around 77.2% (34 cases). In conclusion, the younger is the case, the higher the positive rate is. However, most of them probably are carriers. Newborns weighed more than 3 Kg and infants older than one month were not treated by erythromycin, but with supportive methods, the clinical symptoms and signs still improved gradually. However in newborn with symptoms of respiratory problem antibiotic treatment is usually indicated, and at present erythromycin is very effective.

Antigens, Bacterial↗