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PubMed1997

Comparison of energy expenditure elevations after submaximal and supramaximal running.

Although exercise intensity has been identified as a major determinant of the excess postexercise oxygen consumption (EPOC), no studies have compared the EPOC after submaximal continuous running and supramaximal interval running. Eight male middle-distance runners [age = 2.1 +/- 3.1 (SD) yr; mass = 67.8 +/- 5.1 kg; maximal oxygen consumption (VO2max) = 69.2 +/- 4.0 ml.kg-1.min-1] therefore completed two equated treatments of treadmill running (continuous running: 30 min at 70% VO2max; interval running: 20 x 1-min intervals at 105% VO2max with intervening 2-min rest periods) and a control session (no exercise) in a counter-balanced research design. The 9-h EPOC values were 6.9 +/- 3.8 and 15.0 +/- 3.3 liters (t-test:P = 0.001) for the submaximal and supramaximal treatments, respectively. These values represent 7.1 and 13.8% of the net total oxygen cost of both treatments. Notwithstanding the higher EPOC for supramaximal interval running compared with submaximal continuous running, the major contribution of both to weight loss is therefore via the energy expended during the actual exercise.

Adult↗

PubMed2001

Consumer-run service participation, recovery of social functioning, and the mediating role of psychological factors.

OBJECTIVE: This study examined the relationship between participation in consumer-run services and recovery of social functioning among persons diagnosed as having serious mental illness. It also assessed the role of psychological factors in mediating this relationship. METHODS: Research questions investigated were whether involvement in consumer-run services is positively associated with recovery when premorbid and demographic factors are controlled for, whether psychological factors are positively associated with recovery irrespective of involvement in consumer-run services, and whether the relationship between involvement in consumer-run services and recovery is mediated by the psychological factors. The factors examined were self-efficacy, hopefulness, and active coping strategies. Sixty participants with a past or present diagnosis of schizophrenia, schizoaffective disorder, or bipolar disorder and at least one past psychiatric hospitalization were recruited from a community mental health center and two consumer-run programs. Data were collected on hopefulness, self-efficacy, coping strategies, social functioning, and premorbid and demographic characteristics. RESULTS: Findings indicated that participants involved in consumer-run services had better social functioning than those involved only in traditional mental health services, that psychological variables were significantly associated with social functioning, and that the relationship between involvement in consumer-run services and social functioning was partly mediated by the use of more problem-centered coping strategies. Premorbid and demographic factors did not account for the relationship between psychosocial variables and social functioning, although education was a significant predictor of social functioning. CONCLUSIONS: The findings support the view that psychosocial factors may play a role in facilitating good community adjustment for individuals diagnosed as having serious mental illness.

Adaptation, Psychological↗

PubMed2002

Brainstem lateral line responses to sinusoidal wave stimuli in still and running water.

The fish lateral line consists of superficial and canal neuromasts. In still water, afferent fibers from both types of neuromast respond equally well to a sinusoidally vibrating sphere. In running water, responses to a vibrating sphere of fibers innervating superficial neuromasts are masked. In contrast, responses of fibers innervating canal neuromasts are barely altered. It is not known whether this functional subdivision of the peripheral lateral line is maintained in the brain. We studied the effect of running water on the responses to a 50 Hz vibrating sphere of single units in the medial octavolateralis nucleus (MON) in goldfish Carassius auratus. The MON is the first site of central processing of lateral line information. Three types of units were distinguished. Type I units (N=27) were flow-sensitive; their ongoing discharge rates either increased or decreased in running water, and as a consequence, responses of these units to the vibrating sphere were masked in running water. Type II units (N=7) were not flow-sensitive; their ongoing discharge rates were comparable in still and running water, so their responses to the vibrating sphere were not masked in running water. Type III units (N=7) were also not flow-sensitive, but their responses to the vibrating sphere were nevertheless masked in running water. Although interactions between the superficial and canal neuromast system cannot be ruled out, our data indicate that the functional subdivision of the lateral line periphery is maintained to a large degree at the level of the medial octavolateralis nucleus.

Animals↗

PubMed2007

Effect of running training on DMH-induced aberrant crypt foci in rat colon.

PURPOSE: We examined the effects of treadmill-running training on the induction of aberrant crypt foci (ACF), which is the first step of colon cancer induction, in the colonic mucosa of rats injected with 1,2-dimethylhydrazine (DMH). METHODS: Four-week-old F344 rats (N = 38) were randomly assigned to training (19 rats) and control (19 rats) groups. After a week, all rats were given DMH (20 mg.kg(-1) body weight) once a week for 2 wk. Running training was started at age 7 wk (speed: 10 m.min(-1), 0% grade, 120 min.d(-1), 5 d.wk(-1)). After 4 wk of training, the rats were sacrificed and the colon was removed, opened, and counted for ACF with 0.2% methylene blue staining. RESULTS: Running training resulted in lower body- (P < 0.01) and adipose fat weight (P < 0.05). The numbers of ACF and total AC were significantly lower in the running training group than in the control group (P < 0.05). The occurrences of one, three, and five aberrant crypts per focus were also significantly lower in the running training group than in the control group (P < 0.05). The ratios of total AC/ACF did not significantly differ between the running training and control groups. CONCLUSIONS: The results of the present investigation suggest that low-intensity running training inhibits the DMH-induced initiation of colon ACF development.

Animals↗

PubMed2004

A genomic basis for the evolution of vertebrate transcription factors containing amino Acid runs.

We have previously shown that polyAla (A) tract-containing proteins frequently present runs of glycine (G), proline (P), and histidine (H) and that, in their ORFs, GC content at all codon positions is higher than that in the rest of the genome. In this study, we present new analyses of these human proteins/ORFs. We detected striking differences in codon usage for A, G, and P in and out of runs. After dividing the ORFs, we found that 5' halves were richer in runs than 3' halves. Afterward, when removing the runs, we observed that the run-rich halves (grouped irrespectively of their 5' or 3' position) had a marked statistical tendency to have more homo- and hetero-dicodons for A, G, P, and H than the run-poor halves. This suggests that, in addition to the necessary GC-rich genomic background, a specific codon organization is probably required to generate these coding repeats. Homo-dicodons may indeed provide primers for run formation through polymerase slippage. The compositional analysis of human HOX genes, the most polyAla-rich family, and their comparison with their zebrafish homologs, support these hypotheses and suggest possible effects of genomic environment on ORF evolution and organismal diversification.

Amino Acid Sequence↗

PubMed1980

Parallel effects of light signals on the circadian rhythms of running activity and ovulation in rats.

Continuous monitoring of wheel-running activity and determination of the time of ovulation in rats by serial laparotomies revealed that ovulation followed the onset of running at prooestrus by approximately 9 h (range 7--1 h). This temporal relationship held in rats in which the period of the circadian rhythm had been modified (entrained) by daily exposure to 14 h photoperiods, and in rats in dim continuous light whose rhythms were non-entrained (free-running). Knowledge of this temporal relationship between the two rhythms made it possible to give bright light signals at known points in the circadian cycle of the rat and to observe the effects on the timing of running and ovulation in subsequent cycles. Giving daily light signals near the onset of running (i.e. at subjective dusk) delayed, whereas giving signals near the end of running (i.e. at subjective dawn) advanced, the time of running and ovulation in subsequent cycles. These results indicate that in rats exposed to the usual laboratory photoperiod the delaying effect of dusk light and the advancing effect of dawn light balance one another; thus the preovulatory surge of LH occurs at a relatively consistent time at prooestrus.

Activity Cycles↗

PubMed2000

Effects of a hexameric deoxyriboguanosine run conjugation into CpG oligodeoxynucleotides on their immunostimulatory potentials.

CpG oligodeoxynucleotides (ODNs) are promising immunomodulatory agents for treating human diseases and vaccine development. Phosphodiester CpG ODNs were demonstrated to have poor immunostimulatory potentials for cytokine production. However, the conjugation of consecutive deoxyriboguanosine residues, called a dG run, at the 3' terminus of phosphodiester CpG ODNs significantly enhanced TNF-alpha and IL-12 production from mouse splenic dendritic cells (DCs). The optimal induction of cytokine production was achieved by the addition of a hexameric dG (dG6) run. In contrast, the existence of a dG6 run either at the 5' terminus of phosphodiester CpG ODNs or at the 3' terminus of phosphorothioate CpG ODNs diminished CpG-mediated cytokine induction, suggesting that the effects of a dG run depend on its location and the chemical property of the ODN backbone, respectively. In addition, we provided the evidence that the conjugation of a dG6 run caused the structural transformation of CpG ODNs, which facilitates their targeting into mouse APCs such as splenic DCs, B cells, and peritoneal macrophages with a scavenger receptor type A ligand specificity. Among primary APCs, DCs were the most potent for CpG ODN-mediated IL-12 production. Furthermore, we demonstrated that the conjugation of a dG6 run into the 3' terminus of phosphodiester CpG ODNs was crucial for their ability to generate Th1 immunity in vivo. Thus, the conjugation of a dG6 run into phosphodiester CpG ODNs would be an alternative way to optimize their immunostimulatory potentials in vitro and in vivo.

3' Untranslated Regions↗

PubMed1999

Addiction-prone Lewis but not Fischer rats develop compulsive running that coincides with downregulation of nerve growth factor inducible-B and neuron-derived orphan receptor 1.

We have examined the effects of chronic voluntary running for 30 d on the levels of nerve growth factor inducilble-B (NGFI-B) and neuron-derived orphan receptor 1 (NOR1) mRNAs in Fischer and Lewis rats. The aim was to compare the addiction-prone Lewis rat strain to the Fischer strain in a plausible model for natural reward. The Lewis strain ran markedly more than the Fischer strain, as indicated by the length of running per day when given free access to running wheels. Both strains progressively increased their amount of daily running. By day 14, Lewis rats had reached a maximal level corresponding to 10 km/d, which slowly decreased to approximately 8 km/d. Fischer rats ran considerably less, averaging approximately 1. 5 km/d by day 30. After 30 d of running, levels of mRNA encoding NGFI-B and Nor1 were decreased in cerebral cortex in Lewis but not Fischer rats. The downregulation of NGFI-B mRNA in Lewis rats could not be attenuated by the opioid receptor antagonist naloxone. Instead, naloxone by itself downregulated NGFI-B in striatum and cerebral cortex in both strains. In contrast, naloxone had no effect on Nor1 mRNA levels, although the running-induced downregulation of Nor1 was, in most cases, attenuated by naloxone. Data from the present study suggest that the same genetic factors contributing to the drug addiction-prone behavior of Lewis rats also control the excessive running behavior and that this coincides with downregulation of transcription factors of the NGFI-B family.

Animals↗

PubMed2002

Delta FosB regulates wheel running.

DeltaFosB is a transcription factor that accumulates in a region-specific manner in the brain after chronic perturbations. For example, repeated administration of drugs of abuse increases levels of DeltaFosB in the striatum. In the present study, we analyzed the effect of spontaneous wheel running, as a model for a natural rewarding behavior, on levels of DeltaFosB in striatal regions. Moreover, mice that inducibly overexpress DeltaFosB in specific subpopulations of striatal neurons were used to study the possible role of DeltaFosB on running behavior. Lewis rats given ad libitum access to running wheels for 30 d covered what would correspond to approximately 10 km/d and showed increased levels of DeltaFosB in the nucleus accumbens compared with rats exposed to locked running wheels. Mice that overexpress DeltaFosB selectively in striatal dynorphin-containing neurons increased their daily running compared with control littermates, whereas mice that overexpress DeltaFosB predominantly in striatal enkephalin-containing neurons ran considerably less than controls. Data from the present study demonstrate that like drugs of abuse, voluntary running increases levels of DeltaFosB in brain reward pathways. Furthermore, overexpression of DeltaFosB in a distinct striatal output neuronal population increases running behavior. Because previous work has shown that DeltaFosB overexpression within this same neuronal population increases the rewarding properties of drugs of abuse, results of the present study suggest that DeltaFosB may play a key role in controlling both natural and drug-induced reward.

Animals↗

PubMed2002

Loss of photic entrainment and altered free-running circadian rhythms in math5-/- mice.

Mammalian free-running circadian rhythms are entrained to the external light/dark cycle by photic signaling to the suprachiasmatic nuclei via the retinohypothalamic tract (RHT). We investigated the circadian entrainment and clock properties of math5-/- mutant mice. math5 is a critical regulator of retinal ganglion cell development; math5-/- mice show severe optic nerve hypoplasia. By anterograde cholera toxin B tracing, we find that math5-/- mice do not develop an identifiable RHT pathway. This appears to be attributable to agenesis or dysgenesis of the majority of RHT-projecting retinal ganglion cells. math5-/- mice display free-running circadian rhythms with a period approximately 1 hr longer than B6/129 controls (24.43 +/- 0.10 vs 23.62 +/- 0.19 hr; p < 0.00001). The free-running period of heterozygote mice is indistinguishable from that of controls. math5-/- mice show no entrainment to light/dark cycles, whereas heterozygote mice show normal entrainment to both 12 hr light/dark cycles and to a 1 hr skeletal photoperiod. math5-/- mice show reduced ability to entrain their rhythms to the nonphotic time cue of restricted running wheel access but demonstrate both free-running behavior and entrained anticipation of wheel unlocking in these conditions, suggesting the presence of a second diurnal oscillatory system in math5-/- animals. These results demonstrate that retinal ganglion cell input is not necessary for the development of a free-running circadian timekeeping system in the suprachiasmatic nucleus but is important for both photic entrainment and determination of the free-running period.

Animals↗

PubMed2005

Treadmill running promotes functional recovery and decreases brain-derived neurotrophic factor mRNA expression following sciatic crushed nerve injury in rats.

AIM: Peripheral nerve injuries are commonly encountered clinical problems and often result in severe functional deficits. In the present study, the effects of treadmill running on the recovery rate of locomotor function and the expression of brain-derived neurotrophic factor (BDNF) mRNA following sciatic crushed nerve injury in rats were investigated. METHODS EXPERIMENTAL DESIGN: Comparative investigation was performed over 14 days. SETTING: Experimental animal laboratory. PARTICIPANTS: 24 male Sprague-Dawley rats weighing 200+/-10 g. Animals were randomly assigned into 3 groups: the sham-operation group, the sciatic nerve injury group, and the sciatic nerve injury and running group. INTERVENTIONS: The right sciatic nerve was crushed for 30 s using a surgical clip. Rats of the running group were made to run on a treadmill for 30 min once a day for 12 consecutive days. MEASURES: Functional recovery was analyzed using a walking track analysis which can be quantified with the sciatic function index (SFI) and BDNF mRNA expression was analyzed using reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Sciatic crushed nerve injury showed characteristic gait changes showing decrease of SFI value and treadmill running significantly enhanced the SFI value. The level of BDNF mRNA expression was increased following sciatic crushed nerve injury and treadmill running significantly suppressed the sciatic nerve injury-induced increment of BDNF mRNA expression. CONCLUSIONS: It can be suggested that treadmill running after peripheral nerve injury is effective in the functional recovery by inhibition on the over-expression of BDNF mRNA.

Animals↗

PubMed1990

[The relationship between free-running period and monoamines in the SCN].

Free-running period of blinded rats that had kept in a cage with a running wheel varied markedly between 23.4-24.7 h. The free-running period correlated negatively with motor activity (r = -0.61, P less than 0.005). In the SCN, 5-HT concentration correlated positively with motor activity (r = 0.70, P less than 0.005), consequently correlated negatively to the free-running period (r = -0.79, P less than 0.005). Similarly, 5-HIAA content showed weakly negative correlation to the free-running period (r = -0.45, P less than 0.05) but such correlation was never observed in other monoamines and their metabolites examined (NE, DA, DOPAC and HVA). Further, no correlation was seen between free-running period and contents of amines and their metabolites, including 5-HT and 5-HIAA, in the areas tested other than SCN. These facts suggest that motor activity affects the activity of serotonergic system in SCN, resulting in the change in the free-running period.

Animals↗

PubMed1995

High-fat foods overcome the energy expenditure induced by high-intensity cycling or running.

OBJECTIVE: To examine the effects of two types of vigorous exercise [cycling (CYC) and running (RUN)] and diet composition on appetite control. DESIGN: Two studies using separate groups of subjects were used for the two forms of exercise. The studies used a 2 x 2 design with the factors being exercise and diet composition. Therefore both studies had four treatment conditions and used a repeated measures design. SETTING: Both studies took place in the Human Appetite Research Unit at Leeds University. SUBJECTS: Twenty-four lean, healthy males were recruited from the student staff population of Leeds University. INTERVENTIONS: For both studies a control (no-exercise) and a vigorous exercise session (70% VO2 max) was followed by a free-selection lunch comprising high-fat/low-carbohydrate foods or low-fat/high-carbohydrate foods, during which energy and macronutrient intake was monitored. Motivation to eat was measured by visual analogue scales and by the latency to volitional onset of eating. Energy intake for the remainder of the day (outside of laboratory) was monitored by providing the subjects with airline-style food boxes. RESULTS: Both CYC and RUN produced similar effects on appetite responses. Both CYC and RUN induced a transitory suppression of hunger (P < 0.01 and P < 0.05) and a delay to the onset of eating (P < 0.001). Exercise (whether CYC or RUN) had no significant effect on the total amount of food eaten, but there was a significant effect of lunch type. When provided with the high-fat/low-carbohydrate foods energy intake was significantly elevated (CYC: P < 0.001; and RUN: P < 0.0001). Both types of exercise induced a short-term negative energy balance when followed by the low-fat/high-carbohydrate foods (P < 0.001), which was completely reversed (positive energy balance) when subjects ate from the high-fat/low carbohydrate foods. CONCLUSIONS: These results indicate that eating high-fat foods can prevent exercise inducing any (short-term) negative energy balance. Therefore, in order for exercise to have a significant impact on weight control, it is important to consider the energy density of the accompanying diet. Despite the different physiological aspects of cycling and running, they did not display different effects on appetite.

Adult↗

PubMed1993

Oxygen cost of treadmill running in 24-month-old Fischer-344 rats.

The oxygen cost of treadmill running is well documented in young adult rats. However, to our knowledge there are no detailed reports concerning the oxygen cost of treadmill running in senescent rats. Young adult (4 months; N = 8) and senescent (24 months; N = 8) female Fischer-344 rats were tested for O2 cost of treadmill running. One-half of the animals tested in each age group had completed 10 wk of endurance training. Oxygen cost of treadmill running, using a flow-through system, was measured on a per minute basis from 7-10 min of each work bout and averaged. Separate work bouts were performed at 0%, 5%, and 10% grade at 15, 20, 25, and 30 m.min-1. Oxygen cost of treadmill running for trained rats for all speeds and grades were not significantly different (P = 0.77) from untrained animals; thus, data were pooled for oxygen cost of running. Results revealed significantly lower (P < 0.05) VO2 (expressed as ml.kg-1.min-1) for senescent rats vs young adult rats rest and at all comparable treadmill grades and speeds except for 5% grade, 25 m.min-1 and 10% grade, 20 m.min-1. In addition, resting VO2 was significantly lower (P < 0.05) in old rats when compared with young rats. Thus, we conclude that the oxygen cost of treadmill running is lower for 24-month-old rats than in 4-month-old rats except at higher work levels.

Aging↗

PubMed1997

Duplex assessment of run-off before femorocrural reconstruction.

BACKGROUND: This study was a prospective evaluation of colour duplex imaging for the assessment of distal run-off before femorocrural reconstruction. METHODS: Patients with critical ischaemia who required a distal bypass underwent preoperative run-off assessments using dependent Doppler, arteriography and duplex imaging by a vascular surgeon, radiologist and technologist respectively; each was blinded to the findings of the others. Preoperative data were compared with intraoperative clinical findings and completion flow studies/ arteriograms. RESULTS: Forty-three consecutive patients (33 men, ten women; mean age 78 (range 53-95) years; 12 diabetic) undergoing 44 femorocrural reconstructions for critical ischaemia were assessed. The 30-day primary cumulative graft patency for the series was 86 per cent. Dependent Doppler correctly predicted a suitable run-off vessel in 21 limbs but was indeterminate in four and unrecordable in 19. Arteriography correctly predicted a suitable run-off vessel in 32 cases, but was indeterminate in six and failed to demonstrate run-off in three patients. Arteriography suggested an inferior vessel in three cases. Duplex correctly predicted a suitable run-off vessel for all 44 grafts. CONCLUSION: Duplex imaging is superior to arteriography for preoperative assessment of distal run-off for femorocrural reconstruction.

Aged↗

PubMed1994

Effects of free mobilization and low- to high-intensity treadmill running on the immobilization-induced bone loss in rats.

After an immobilization period of 3 weeks, the effects of free remobilization (8 weeks) as well as low- and high-intensity treadmill running on the bone mineral content (BMC) and density (BMD) of the hindlimbs of Sprague-Dawley rats (n = 70) were studied using a dual-energy x-ray absorptiometric scanner. In the low-intensity running program, the rats were allowed to move freely in the cage for 1 week, after which they started to run on a treadmill twice a day for 7 weeks. The speed of the treadmill was 20 cm/s, with an uphill inclination of 10 degrees. The running time was gradually increased from 20 minutes per session to 45 minutes per session. In the high-intensity group, the program was similar, with the exception that the speed of the treadmill was 30 cm/s, with an uphill inclination of 30 degrees. Immobilization for 3 weeks produced a significant BMC and BMD loss in the immobilized left femur and tibia (mean loss 9.6%, p < 0.001) but did not affect the right free limbs. Both low- and high-intensity running restored mineral content in the immobilized limb; however, an average 5% difference (p < 0.05) in mineral content of the right and left limb bones persisted. In the running groups, the values for the immobilized left limbs were at the same level or exceeded (range 3.8-11.6%, p < 0.05-0.01) and those of the free right limbs exclusively exceeded (range 5.3-15.9%, p < 0.05-0.01) the corresponding values of the age-matched control rats.(ABSTRACT TRUNCATED AT 250 WORDS)

Absorptiometry, Photon↗

PubMed1996

beta-Endorphin and adrenocorticotrophin after incremental exercise and marathon running--female responses.

Investigations of exercise-induced increases in beta-endorphin, adrenocorticotropic hormone (ACTH) and cortisol concentration have been carried out mainly in men. Data concerning the female reaction are sparse and less clear. In a comparison between incremental exercise and marathon running 14 experienced female marathon runners volunteered to run to exhaustion according to an incremental treadmill protocol. They ran a marathon 4 weeks later. Blood was analysed for beta-endorphin, ACTH and cortisol concentration immediately prior to the laboratory treadmill test, 3, 30 and 60 min later, as well as prior to the marathon, after 60 min and 120 min of running and 3, 30 min, and 24 h after completion of the run. At each blood collection, lactate concentration, heart frequency and perceived exertion were determined. The mean marathon running time was 3.22 h. Baseline concentrations for beta-endorphin of 22 pmol.l-1 before the marathon and 19 pmol.l-1 before the treadmill exercise increased 1.4-fold 30 min after the marathon and 1.9-fold after the treadmill exercise; for ACTH the baseline of 4.7 and 4.0 pmol.l-1 was increased by 8.3- and 10.3-fold, respectively. Cortisol concentration rose exponentially from a baseline 17 micrograms.dl-1 and peaked at 2.2-fold 30 min after the run, when the maximal concentration also had been reached after the treadmill test, increasing 1.3-fold from a baseline of 21 micrograms.dl-1. The maximal values for cortisol concentration after both exercises differed from each other, while the maxima of ACTH and beta-endorphin concentrations were similar. The ACTH and beta-endorphin concentration declined more slowly during the recovery after the marathon than after the treadmill. Cortisol concentration was below baseline 24 h later. In comparison with men studied earlier, female marathon runners showed higher baseline concentrations and lesser increases in beta-endorphin and lower baseline concentrations and larger increases in ACTH concentration after both types of exercise. The delayed decrease in concentration of the hormones after the marathon was similar in male and female runners.

Adrenocorticotropic Hormone↗

PubMed1982

A maximal multistage 20-m shuttle run test to predict VO2 max.

In order to validate a maximal multistage 20-m shuttle run test for the prediction of VO2 max, 91 adults (32 females and 59 males, aged 27.3 +/- 9.2 and 24.8 +/- 5.5 year respectively and with mean VO2 max (+/- SD) of 39.3 +/- 8.3 and 51.6 +/- 7.8 ml . kg-1 . min-1 respectively) performed the test and had VO2 max estimated by the retroextrapolation method (extrapolation to time zero of recovery of the exponential least squares regression of the first four 20-s recovery VO2 values). Starting at 8 km . h-1 and increasing by 0.5 km . h-1 every 2 min, the 20-m shuttle run test enabled prediction of the VO2 max (y, ml . kg-1 . min-1) from the maximal speed (x, km . h-1) by means of the following regression equation: y = 5.857x - 19.458; r = 0.84 and SEE = 5.4. Later, the multistage protocol was slightly modified to its final version, in which the test started at stage 7 Met and continued with a 1 Met (3.5 ml O2 . kg-1 . min-1) increment every 2 min. Twenty-five of the 91 subjects performed the 20-m shuttle test twice, once on a hard, low-friction surface (vinyl-asbestos tiles) and another time on a rubber floor, as well as a walking maximal multistage test on an inclined treadmill. There was no difference between the means of these tests or between the slopes of the VO2max - maximal speed regressions for the two types of surfaces. The 20-m shuttle run test and another maximal multistage field test involving continuous track running gave comparable results (r = 0.92, SEE = 2.6 ml O2 . kg-1 . min-1, n = 70). Finally, test and retest of the 20-m shuttle run test also yielded comparable results (r = 0.975, SEE = 2.0 ml O2 . kg-1 . min-1, n = 50). It is concluded that the 20-m shuttle run test is valid and reliable test for the prediction of the VO2 max of male and female adults, individually or in groups, on most gymnasium surfaces.

Adult↗