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Results for “AGGLUTININS AND AGGLUTINATION”

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At least 631 records · Page 35Linked to original sources
PubMed1986

Pulmonary reaction associated with transfusion of plasma containing anti-5b.

A patient developed an acute respiratory distress syndrome with lung infiltrates during a transfusion of plasma. The transfused plasma was shown to contain a potent leucocyte agglutinating antibody of IgG type with anti-5b specificity, reactive with the patient's cells. A review of the literature on similar transfusion-related syndromes showed an association with more of less well-defined white cell antibodies, mainly in the donors' plasma. The majority of donors were multiparous women. The anti-5b in our donor was demonstrated only by granulocyte agglutination technique, as previously described antibodies in the five system. A long incubation procedure (20-25 h) seemed to be optimal for granulocyte agglutination.

Aged↗
PubMed1976

Ectogalactosyltransferase. Presence of enzyme and acceptors on the rat lymphocyte cell surface.

Experiments are described to demonstrate the existence of ectogalactosyltransferase activity on the lymphocyte surface. The procedures described enable us to exclude the possibility of misleading results due to precursor hydrolysis and intracellular utilization of the free galactose. This depicted transferase is able to catalyse the transfer of a galactosyl residue from UDP-galactose to a nonphagocytosable exogenous acceptor and to endogenous membrane acceptors. The cells galactosylated in this way acquired new agglutinating properties with soybean agglutinin, which proves the external position of the galactosyl residues incorporated on the cell surface.

Animals↗
PubMed1984

Monoclonal antibodies to the h1 agglutinogen from Staphylococcus aureus 17A. Serological testing with type strains.

Four monoclonal antibodies to the h1 agglutinogen were produced by conventional means, and slide agglutination of S. aureus type strains was performed with protein A affinity purified IgG1 antibodies. In accordance with Oeding's serotype system the type strains 17A and 670 were strongly and consistently agglutinated. In addition, however, several of the remaining twelve type strains investigated showed varying reaction patterns. Our results indicate that the h1 agglutinogen may be more widely distributed among S. aureus strains than previously assumed.

Agglutination Tests↗
PubMed1975

A mechanism for prozone formation in the complement fixation test for bovine brucellosis.

Complement fixation (CF) by bovine IgG1 or IgM antibodies to Brucella abortus was inhibited by specific non-complement-fixing antibodies of the IgG2 subclass. This inhibition may account for the appearance of prozones in CF titrations of some antiserums, and for the occurrence of serums which are positive to the Rose Bengal test, but negative to the CF test.

Agglutination Tests↗
PubMed1974

Antibody responses in rabbits to injections of whole cell, flagella, and flagellin preparations of cholera and noncholera vibrios.

Sera prepared by the injection of living cells, killed cells, flagella, or flagellins of vibrios into rabbits were examined for antibodies which cross-reacted with cholera and noncholera vibrio strains. Antisera to Vibrio cholerae strains agglutinated heterologous V. cholerae strains. They did not agglutinate strains of noncholera vibrios. Sera to noncholera strains agglutinated homologous strains only. There was no evidence that a common antigen was present in the organisms tested. The proposal for a "cholera group of vibrios" based upon sharing of an H antigen between cholera and some noncholera vibrios is held as untenable. It is indicated, however, that the use of serotyping of vibrios could be used as an added tool for better identification or description of vibrios.

Agglutination Tests↗
PubMed1974

Immunological study of typhoid: immunoglobulins, C3, antibodies, and leukocyte migration inhibition in patients with typhoid fever and TAB-vaccinated individuals.

The development of humoral and cell-mediated immune responses to Salmonella typhi antigens and immunoglobulin and C(3) levels were determined in patients suffering from typhoid fever, TAB-vaccinated individuals, and appropriate controls. In 45 patients with typhoid, a significant elevation of immunoglobulin M (IgM) level was noted from the first week of illness onwards. Eighteen TAB-vaccinated persons also showed a significant elevation of IgM levels. In typhoid sera, the anti-O and anti-H antibodies were mostly 2-mercaptoethanol (2-ME) sensitive. The rise of IgM level correlated well with the 2-ME-sensitive anti-O and anti-H antibodies seen in typhoid patients. The anti-O antibodies in the TAB-vaccinated group were almost entirely 2-ME sensitive, but both 2-ME-sensitive and -resistant anti-H antibodies were detected in the TAB group. A marked increas in C(3) level was also noted in patients with typhoid. The cell-mediated immunity (CMI), as measured by leukocyte migration inhibition tests, was demonstrable in 15 of 22 patients with typhoid. On the other hand, only 8 of the 20 normal subjects, 5 of the 16 fever control cases, and 6 of the 18 TAB-vaccinated individuals gave a positive CMI. The latter three groups were comparable with each other but were significantly different from the typhoid patients. It was concluded that TAB-vaccination did not induce CMI even though it induced the development of antibodies, the latter being comparable with those of the patients with typhoid. The significance of these findings is discussed.

Agglutination Tests↗
PubMed1979

Surface mucopolysaccharides of Treponema pallidum.

The viscous mucoid fluid that accumulates within syphilitic lesions may be due to breakdown of host tissue during infection, or may be synthesized by Treponema pallidum. Experiments were performed to investigate the acidic mucopolysaccharides that occur at the surface of T. pallidum (Nichols strain). These mucopolysaccharides were demonstrated by reaction with acidified bovine serum albumin and by agglutination with wheat germ agglutinin and soybean agglutinin. The polycations ruthenium red and toluidine blue influenced treponemal survival. Concentrations of both compounds at 200 mug/ml inhibited survival, whereas concentrations at 0.1mug/ml enhanced survival. The mucopolysaccharide concentration within the mucoid fluid that accumulates during intratesticular infection was determined by reaction with acidified bovine serum albumin; it ranged from 10,000 mug/ml to less than 8 mug/ml. The addition of this mucoid fluid to treponemal suspensions resulted in differing effects on T. pallidum survival. Some preparations were inhibitory, and others were stimulatory. Commercial preparations of hyaluronic acid and chondroitin sulfate at 400, 200, 100, and 50 mug/ml were detrimental to treponemal survival. The organisms exhibited pronounced clumping in the presence of the higher concentrations of hyaluronic acid. These clumps of treponemes were comprised of mucopolysaccharides as shown by acidified bovine serum albumin and toluidine blue reactions and by hyaluronidase degradation. Results are discussed in terms of the derivation and potential role of acidic mucopolysaccharides at the surface of T. pallidum.

Animals↗
PubMed1983

Modulation of Bordetella pertussis by nicotinic acid.

Growth of Bordetella pertussis in a high concentration of nicotinic acid (NA) had a modulating effect on several properties and activities of the bacteria. Compared with normally grown cells, those grown in a high concentration of NA had reduced capacity for taking up both NA and nicotinamide (ND); they had reduced adenylate cyclase activity and showed loss of agglutinogen factors 2 and 3, but an increase in factor 1. By contrast, cells grown in a high concentration of ND showed only a slightly decreased capacity for uptake of ND and none of the other changes. Modulation of B. pertussis by NA varied with the strain and culture conditions and appeared to be distinct from the antigenic modulation induced by high Mg2+ in the culture medium. Evidence is presented for the association of a small proportion of the extracytoplasmic adenylate cyclase with the outer membrane of B. pertussis.

Adenylyl Cyclases↗
PubMed1974

Enhancement activity of homologous anti-staphylococcal sera in experimental staphylococcal synovitis of chicks: a possible role of immune adherence antibodies.

Homologous anti-staphylococcal sera passively potentiated the development of experimental staphylococcal synovitis infection of chickens. These antisera obtained from chickens hyperimmunized with live Staphylococcus aureus were selected according to their immune adherence and agglutinating properties. While the agglutinins rose steadily for 11 weeks during the hyperimmunization schedule, the immune adherence antibody titers reached a peak at 8 weeks and dropped to almost zero 2 to 4 weeks later. The enhancement activity of these antisera was associated with a relatively high level of immune adherence antibodies and seemed not to be correlated with their agglutinin titers.

Animals↗
PubMed1977

Leprosy and the serodiagnostic test for tuberculosis.

Sera from patients with leprosy agglutinated killed H37Rv Mycobacterium tuberculosis to varying degrees. Higher titres were found in association with active disease but there was no difference between patients at the lepromatous or at the tuberculoid ends of the clinical scale. A sharp rise in titre occurred during the active phase of an episode of erythema nodosum leprosum.

Agglutination Tests↗