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PubMed2001

Survival of Coxiella burnetii within free-living amoeba Acanthamoeba castellanii.

OBJECTIVE: To determine whether Coxiella burnetti is able to survive within free-living amoeba. METHODS: When C. burnetii was co-cultivated with the free-living amoeba species Acanthamoeba castellanii. RESULTS: Viable bacteria were detected for 18 days. Microscopic studies confirmed the presence of bacteria within vacuoles, some of which appeared to be spore-like forms. CONCLUSION: These results indicate that free-living amoebae could provide an intracellular niche for the spore formation and survival of C. burnetii in the environment.

Acanthamoeba↗
PubMed1993

Purification of Coxiella burnetii antigen by gel-chromatography for use in enzyme-linked immunosorbent assay (ELISA).

Cell wall antigens from Coxiella burnetti were extracted by heat, ultrasonication, phenol, SDS or sodium deoxycholate. These antigens were subsequently purified by gel-chromatography yielding molecular weights of 700 and 270 KD. Using gel-chromatographical purification of antigens extracted by heat, ultrasonication or phenol Coxiella antigens nearly devoid of contaminations could be obtained. Of these highly purified antigens heat extracted antigen was treated with proteases, lysozyme or metaperiodate exemplarily. These treatments did neither destroy nor change its antigenic activity, except for the treatment with metaperiodate reducing the antigenic activity significantly. In further experiments heat extracted and gel-chromatographically purified Coxiella antigen was used for testing bovine sera in ELISA.

Animals↗