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At least 631 records · Page 35Linked to original sources

PubMed2005

A large-scale screen for artificial selection in maize identifies candidate agronomic loci for domestication and crop improvement.

Maize (Zea mays subsp mays) was domesticated from teosinte (Z. mays subsp parviglumis) through a single domestication event in southern Mexico between 6000 and 9000 years ago. This domestication event resulted in the original maize landrace varieties, which were spread throughout the Americas by Native Americans and adapted to a wide range of environmental conditions. Starting with landraces, 20th century plant breeders selected inbred lines of maize for use in hybrid maize production. Both domestication and crop improvement involved selection of specific alleles at genes controlling key morphological and agronomic traits, resulting in reduced genetic diversity relative to unselected genes. Here, we sequenced 1095 maize genes from a sample of 14 inbred lines and chose 35 genes with zero sequence diversity as potential targets of selection. These 35 genes were then sequenced in a sample of diverse maize landraces and teosintes and tested for selection. Using two statistical tests, we identified eight candidate genes. Extended gene sequencing of these eight candidate loci confirmed that six were selected throughout the gene, and the remaining two exhibited evidence of selection in the 3' portion of each gene. The selected genes have functions consistent with agronomic selection for nutritional quality, maturity, and productivity. Our large-scale screen for artificial selection allows identification of genes of potential agronomic importance even when gene function and the phenotype of interest are unknown.

Agriculture↗

PubMed2006

Chloroplast diversity in the genus Malus: new insights into the relationship between the European wild apple (Malus sylvestris (L.) Mill.) and the domesticated apple (Malus domestica Borkh.).

To unravel the relationship between the European wild apple, Malus sylvestris (L.) Mill., and its domesticated relative M. domestica Borkh., we studied chloroplast DNA variation in 634 wild and 422 domesticated accessions originating from different regions. Hybridization between M. sylvestris and M. domestica was checked using 10 nuclear microsatellites and a Bayesian assignment approach. This allowed us to identify hybrids and feral plants escaped from cultivation. Sixty-eight genotypes belonging to 12 other wild Malus species, including 20 M. sieversii (Ledeb.) Roem. accessions were also included in the analysis of chloroplast diversity. Marker techniques were developed to type a formerly described duplication and a newly detected transversion in the matK gene. Chloroplast DNA variation was further investigated using PCR-RFLP (Polymerase Chain Reaction-Random Fragment Length Polymorphism), and haplotypes were constructed based on all mutational combinations. A closer relationship than presently accepted between M. sylvestris and M. domestica was established at the cytoplasmic level, with the detection of eight chloroplast haplotypes shared by both species. Hybridization between M. sylvestris and M. domestica was also apparent at the local level with sharing of rare haplotypes among local cultivars and sympatric wild trees. Indications of the use of wild Malus genotypes in the (local) cultivation process of M. domestica and cytoplasmic introgression of chloroplast haplotypes into M. sylvestris from the domesticated apple were found. Only one of the M. sieversii trees studied displayed one of the three main chloroplast haplotypes shared by M. sylvestris and M. domestica. This is surprising as M. sieversii has formerly been described as the main maternal progenitor of the domesticated apple. This study hereby reopens the exciting discussion on the origin of M. domestica.

Base Sequence↗

PubMed1997

Domestic mites from an acarologic perspective.

Knowledge of the taxonomic, physiologic, biologic, and ecologic characteristics of allergenic domestic mites contributes to the understanding of the causation of many cases of allergic asthma, and to the measures to be taken to control excessive mite occurrence in homes. Domestic mites, i.e., the combined group of storage mites living in the home environment and of pyroglyphid house-dust mites, belong to the subclass of the acari. This comprises also many other mites of medical and economic importance, together with thousands of free-living mites. Because of some species-specific properties of their allergenic products, it is important that the producing mite is always correctly identified. In spite of their small size (approx. 0.5 mm), domestic mites have well-developed and elaborate systems of respiration, digestion, and water balance, enabling them to live and survive in the various habitats of the home environment. Knowledge of the effects of the various and changing ecologic conditions in the home environment is helpful in developing strategies to prevent the establishment and growth of large populations of allergenic domestic mites.

Animals↗

PubMed1998

Domestic violence among patients at two rural health care clinics: prevalence and social correlates.

Despite a growing body of knowledge concerning family abuse, there is little research focusing on domestic violence in rural settings. Likewise, there is a paucity of research on family abuse among Hispanics. This study examined the prevalence of spousal abuse among predominately low income Hispanic patients of two rural health care clinics. The correlation of certain social factors to domestic violence also was examined. Survey data was collected from 155 adult female patients. Findings revealed a 19% prevalence rate. This rate corresponds to recent measures of domestic abuse nationwide. A bivariate analysis showed a significant positive relationship between spousal abuse and drug/alcohol use by victims' intimate partners. Abuse also was significantly related to the community location of the health care clinics. The article concludes by discussing implications of the study for rural domestic violence intervention, treatment, and research.

Adult↗

PubMed1999

History of domestic violence among male patients presenting to an urban emergency department.

OBJECTIVE: To establish the prevalence of domestic violence committed by women against male patients presenting to an urban ED for any reason. METHODS: This was a prospective survey in which male patients of legal age presenting to the ED over a 13-week period were interviewed. Patients answered a series of six questions adapted from the George Washington University Universal Violence Prevention Screening Protocol. Patients who could not speak English, those refusing to participate, those unable to give informed consent, and those meeting regional criteria for major trauma were excluded. RESULTS: Of 866 male patients interviewed, 109 (12.6%) had been the victims of domestic violence committed by a female intimate partner within the preceding year. Victims were more likely to be younger, single, African American, and uninsured. The most common forms of assault were slapping, grabbing, and shoving (60.6% of victims). These were followed by choking, kicking, biting, and punching (48.6%), or throwing an object at the victim (46.8%). Thirty-seven percent of cases involved a weapon. Seven percent of victims described being forced to have sex. Nineteen percent of victims contacted the police; 14% required medical attention; 11% pressed charges or sought a restraining order; and 6% pursued follow-up counseling. CONCLUSIONS: Almost 13% of men in this sample population had been victims of domestic violence committed by a female intimate partner within the previous year. Further attention to the recognition and management of domestic violence committed by women against men may be warranted.

Adolescent↗

PubMed2002

Domestic violence: what do nurse practitioners think?

PURPOSE: To examine factors that influence nurse practitioners' (NPs) ability to incorporate universal domestic violence screening practices (e.g., asking, identifying, referring and reporting) into their practices. DATA SOURCES: A stratified random survey of certified NPs in New York state was conducted in 1999. There were 118 family, women's health, OB/GYN, and adult NPs in the survey. Chi-square and ANOVA were used to analyze the data. CONCLUSIONS: There were significant differences in the domestic violence screening practices among women's health, OB/GYN, adult, and family NPs. Women's health and OB/GYN NPs were more likely to ask screening questions and identify victims of domestic violence than their other NP counterparts. IMPLICATIONS FOR PRACTICE: There is a need to identify strategies that encourage all NPs to incorporate universal domestic violence screening behaviors into their practices.

Adolescent↗

PubMed2006

Rice domestication by reducing shattering.

Crop domestication frequently began with the selection of plants that did not naturally shed ripe fruits or seeds. The reduction in grain shattering that led to cereal domestication involved genetic loci of large effect. The molecular basis of this key domestication transition, however, remains unknown. Here we show that human selection of an amino acid substitution in the predicted DNA binding domain encoded by a gene of previously unknown function was primarily responsible for the reduction of grain shattering in rice domestication. The substitution undermined the gene function necessary for the normal development of an abscission layer that controls the separation of a grain from the pedicel.

Alleles↗

PubMed2006

Long hours in paid and domestic work and subsequent sickness absence: does control over daily working hours matter?

OBJECTIVES: To explore the associations of working hours (paid, domestic, commuting, and total) with sickness absence, and to examine whether these associations vary according to the level of employee control over daily working hours. METHODS: Prospective cohort study among 25 703 full-time public sector employees in 10 towns in Finland. A survey of working hours and control over working hours was carried out in 2000-01. The survey responses were linked with register data on the number of self-certified (< or =3 days) and medically certified (>3 days) sickness absences until the end of 2003. Poisson regression analyses with generalised estimating equations were used to take into account the fact that the employees were nested within work units. Adjustments were made for work and family characteristics and health behaviour. The mean follow-up period was 28.1 (SD 8.1) months. RESULTS: Long domestic and total working hours were associated with higher rates of medically certified sickness absences among both genders. In contrast, long paid working hours were associated with lower rates of subsequent self-certified sickness absences. Long commuting hours were related to increased rates of sickness absence of both types. Low control over daily working hours predicted medically certified sickness absences for both the women and men and self-certified absences for the men. In combinations, high control over working hours reduced the adverse associations of long domestic and total working hours with medically certified absences. CONCLUSIONS: Employee control over daily working hours may protect health and help workers successfully combine a full-time job with the demands of domestic work.

Cohort Studies↗

PubMed1985

Campylobacter infection in domestic dogs.

Campylobacter jejuni, a recently recognised human enteric pathogen, was recovered from the faeces of 21.7 per cent of domestic dogs with diarrhoea as compared with only 3.1 per cent of normal healthy dogs. The recovery rate from non-diarrhoeic, but unhealthy, domestic dogs was 6.7 per cent. The differences in the incidence of C jejuni between breeds was not statistically significant. The majority of isolations were from puppies between birth and six months old. The incidence of C jejuni in domestic dogs did not appear to be sex specific. Domestic canine infections with C jejuni could possibly be an important source of human infection, especially to those in close contact.

Animals↗

PubMed2005

Efficacy of an inactivated feline panleucopenia virus vaccine against a canine parvovirus isolated from a domestic cat.

Canine parvovirus type 2a (CPV-2a) and type 2b (CPV-2b) have recently been isolated from cats throughout the world, and CPV-2b strain FP84 has been reported to be virulent in domestic cats. Although live feline panleucopenia virus (FPLV) vaccines protect domestic cats from CPV infection, the efficacy of inactivated FPLV vaccines has not been established. In this study, two domestic cats were vaccinated with a commercial inactivated FPLV vaccine and challenged with CPV-2b strain FP84 isolated from a domestic cat. The cats were protected against CPV-2b strain FP84 infection and their clinical signs were suppressed, although the two unvaccinated cats showed the typical clinical signs of parvovirus infection.

Animals↗

PubMed2003

AFLP data and the origins of domesticated crops.

Amplified fragment length polymorphism (AFLP) datasets have been used to construct neighbor-joining trees from which monophyletic origins for crops such as einkorn wheat, barley, and emmer wheat have been inferred. We simulated several different multiple domestication scenarios for an imaginary cereal crop and examined the resulting domesticated populations. The simulations showed that the population biology aspects of the domestication process can result in independently domesticated populations merging in such a way that a monophyletic origin is erroneously inferred when the resulting population is examined by AFLP genotyping a nd neighbor-joining analysis. The resultsbring into question the use of this method to infer the origins of real crops.

Cluster Analysis↗

PubMed2000

Differential stress coping in wild and domesticated sea trout.

Offspring of wild and sea-ranched (domesticated) sea trout (Salmo trutta) originating from the same river, were reared under identical hatchery conditions from the time of fertilization. At one year of age individual fish were exposed to two standardized stressors; transfer to a novel environment, with or without a simultaneous predator exposure. Blood plasma concentrations of glucose and cortisol were analyzed along with brain levels of dopamine (DA), 3,4-hydroxyphenylacetic acid (DOPAC, a major DA metabolite), serotonin (5-hydroxytryptamine, 5-HT), and 5-hydroxyindoleacetic acid (5 HIAA, a major 5-HT metabolite). Transfer to a novel environment, alone as well as in combination with predator exposure, resulted in elevated plasma concentrations of glucose and cortisol. Moreover, exposure to these stressors resulted in elevated brain levels of 5-HT and 5-HIAA, as well as elevated brain 5-HIAA/5-HT and DOPAC/DA ratios. Wild trout displayed significantly higher post stress plasma glucose levels than domesticated fish. Similarly, following stress, brain 5-HIAA/5-HT and DOPAC/DA ratios were significantly higher in wild than in domesticated fish. These differences were not caused by differences in brain levels of 5-HIAA and DOPAC, but instead by differences in brain 5-HT and DA concentrations. These results suggest that domestication results in attenuated stress responses in trout, and that alterations in brain monoamine neurotransmission are part of this effect.

3,4-Dihydroxyphenylacetic Acid↗

PubMed1997

Sensitivity and behavioral responses to the pheromone androstenone are not mediated by the vomeronasal organ in domestic pigs.

Based largely on results of studies of laboratory rodents, the vomeronasal or accessory olfactory system is believed to function mainly in social communication, mediating the effects of stimuli such as urine or glandular secretions on the behavior or endocrine response of conspecifics. In the domestic pig (Sus scrofa), the steroid androstenone has been identified as a pheromone that facilitates expression of both attraction to the male and a receptive mating stance in estrous females. Though the domestic pig is one of the few vertebrate species in which the identity of a compound that functions as a pheromone is known, the role of the vomeronasal system in domestic pigs has never been investigated. We have examined the role of the vomeronasal organ in mediating the pheromonal effects of androstenone in pigs. In addition, we have examined the structure of the vomeronasal organ at the gross and light-microscopic levels. The vomeronasal organ appears functional, with sensory epithelium lining the medial wall, and has access to stimuli from both the oral and nasal cavities. To determine whether the vomeronasal organ is necessary for androstenone detection or attraction or receptive behavior in female pigs, access to the vomeronasal organ was blocked with surgical cement, and androstenone detection threshold and sexual behavior were measured. Experimental animals did not differ significantly in androstenone sensitivity, measured behaviorally, from untreated controls. Vomeronasal organ-blocked animals also did not differ from untreated controls in either androstenone-mediated receptive standing behavior or attraction to the odor of androstenone. We conclude that in the domestic pig, the vomeronasal organ is not necessary for androstenone detection or androstenone-mediated sexual behavior in estrous females.

Animals↗

PubMed1997

A hospital-based domestic violence group.

OBJECTIVE: This study of a public-hospital-based drop-in group for women who were victims of domestic violence sought to determine whether the group attracted clients from the target population of patients in the medical system, to identify characteristics of the battered women attending the group, and to examine whether the group shared the same characteristics as battered women who were evaluated in other contexts within the medical system. METHODS: Fifty-nine clients attending a domestic violence group at an urban public hospital completed questionnaires on referral sources, demographic characteristics, needs, and satisfaction. Included for comparison were clinical data on referral sources and demographic characteristics for 224 battered women evaluated by clinical social workers at the hospital and affiliated clinics. RESULTS: Referral patterns differed for the two groups: the majority of the social work cases were referred from the emergency room, and the majority of the referrals to the domestic violence group were from outside agencies, informal sources, and the hospital's inpatient units. A greater proportion of women attending the group were white, divorced or separated, and no longer living with their partners. Among the social work cases, the women were more likely to be ethnic minorities, single, and still living with their partners. CONCLUSIONS: The domestic violence group intervention attracted a different subgroup of battered women than did the social work intervention, which was likely due to differences in readiness to initiate change and to cultural barriers to group participation.

Adult↗

PubMed1997

Regulation and resistance: strategies of migrant domestic workers in Canada and internationally.

This article "is divided into three parts. The first part locates the Canadian migrant domestic/live-in caregiver program in the global context of domestic worker migration, and provides data on its scope. The second part addresses the obstacles to protection of domestic worker rights in the regulatory scheme composed of policies and laws of sending and receiving societies. The third part discusses some of the strategies employed by migrant domestic workers and their advocates to resist exploitation, and to attempt to provide meaningful rather than merely symbolic rights for migrant domestics."

Americas↗

PubMed2000

Dysfunctional domesticity: female insanity and family relationships among the West Riding poor in the mid-nineteenth century.

"Dysfunctional domesticity" contributes to the growing reevaluation of the importance of the history of the family to understanding the history of insanity. Using patient case histories from the West Riding Pauper Lunatic Asylum, this article examines representations of family life among poor in England in the 1830s and 1840s. Among the so-called moral causes of insanity, family relationships held a prominent place. Female patients more than male patients had their mental illnesses attributed to their domestic circumstances: the poverty of their home lives, grief over a death of friends and family, love and marital relationships gone wrong, and violence in their homes. The case histories reveal that poor women experienced many pressures in the domestic sphere, and insanity may have been one way to escape dysfunctional domesticity.

England↗

PubMed2005

Culture and domestic violence: transforming knowledge development.

Cultural competence continues to receive limited attention in domestic violence service provision from research to the evaluation of programs. Yet with changing demographics reflecting larger numbers of people of color and increasing needs for more effective responses, it is critical that we change the way we think about domestic violence. Using a feminist framework, this article examines how knowledge has been developed in domestic violence and its consequence related to service provision and perceptions. The article ends with recommendations as to how to better include culturally competent responses in domestic violence.

Black or African American↗

PubMed2005

"For us it is like living in the dark": Ethiopian women's experiences with domestic violence.

This article discusses the experiences of domestic violence among Ethiopian refugees and immigrants in the United States. A subset (n=18) of the larger study sample (N=254) participated in three focus groups with Amharic-speaking survivors of domestic violence who were currently in or had left abusive relationships. The research was conducted through a public health department, University, and community agency partnership. Findings show domestic violence as taking place within a context of immigration, acculturation, and rapid changes in family and social structure. Participants expressed a need for language and culture-specific domestic violence support and advocacy as well as education programs regarding U.S. laws and resources.

Adult↗