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Concomitant oral findings in children after cardiac transplant.

Heart transplantations have been performed in the Centre for Paediatric Heart Surgery at the Justus Liebig University in Giessen (Germany) since 1988. For further consultation and therapy, some affected children subsequently present at the Polyclinic for Paediatric Dentistry. In all these cases problematic oral findings were diagnosed. It was the aim of this study to describe the different findings and to create a concept for avoiding them in the future. Altogether, 10 children with cardiac transplants (three girls, seven boys) were examined and, where necessary, treated. At the time they first presented, they were between 23 and 119 months old (average = 72.2) and had all been operated on (organ transplant) in the first 6 months of life. Each child showed evidence of gingival hyperplasia in the area of the front teeth, which was because of ongoing immunosuppression. In three patients, reactive mucosal caps avoided the age-related penetration of a permanent incisor in the upper jaw. In addition, the primary teeth of six children were affected by caries; in two of these cases, the damage was because of the nursing-bottle syndrome. The results show that any infant with cardiac transplant has to be subjected to careful dental examination and offered comprehensive medical care in cooperation with the competent centre for child cardiac surgery. Furthermore, the parents of such children should be informed prior to transplantation about the possibility of subsequent findings and how these findings can be either avoided or, if necessary, adequately treated.

Child↗

Extraction as a treatment alternative follows repeated trauma in a severely handicapped patient.

Handicapped patients with protruding maxillary incisors are prone to repeated dental trauma. A 13-year-old girl with cerebral palsy, severe mental retardation and seizure disorder was referred to our department for restoring the traumatized anterior teeth. Despite drug combination, the frequency of seizure attack was around 10 times a month. The oral examination showed multiple caries, gingival hyperplasia, class II malocclusion with 14 mm overjet and deep overbite. During the first 3 years of a 7-year follow-up period, six episodes of anterior tooth trauma due to seizure attack occurred. The trauma-related treatment performed included endodontic therapy, multiple composite restorations, apical repositional flap, and finally extraction of all four upper incisors with fabrication of a semi-fixed band-retained denture. The denture restored normal overbite and overjet with improved esthetics. For 4 years following the fabrication of denture, no trauma occurred to the anterior teeth in later seizure attacks. Considering inadequate control of seizure disorder, little ability of the patient to receive comprehensive orthodontic treatment, poor prognosis of restorations, and possible future injuries, the removal of non-functional, nonesthetic, trauma-susceptible incisor teeth can be justified as an alternative to tooth preservation.

Adolescent↗

Alkaline phosphatase activity in cultured cells of human healthy and hyperplastic gingivae: a preliminary study.

The present study compared the alkaline phosphatase (ALP) expression in cultured cells derived from different types of gingival hyperplasia. Tissue biopsies were obtained from patients with clinically non-inflamed, enlarged gingivae of idiopathic gingivofibromatosis (IGF) and phenytoin-induced hyperplasia (PHG). Fibroblasts obtained from the gingivae or periodontal ligaments (PDL) of the same or different healthy subjects were used as controls. Fibroblasts were plated and the ALP activity and total amount of DNA per well were studied in each isolated cell type at 4, 7 and 9 days of culture. The results revealed that fibroblasts derived from IGF tissue synthesized a large amount of ALP by day 7 and day 9 of culture. The amount of ALP activity was cell density-dependent and the strongest expression of enzyme activity was noted after cell confluence. In contrast, only very low ALP activity was detected in cells derived from normal gingivae and PHG. Cytochemical examination of these cells revealed similar observations. The fact that fibroblasts derived from PHG tissue did not exhibit ALP in vitro suggests that there was a shift of cell phenotype in this tissue. The increased expression of ALP in IGF cells may imply a developmental trend toward osteoblastic phenotypes.

Alkaline Phosphatase↗

Effects of cyclosporin A on alveolar bone: an experimental study in the rat.

BACKGROUND: There have been several investigations on the role of cyclosporin A (CSA) in gingival hyperplasia in both animals and humans. However, less attention has been given to the drug's effect on alveolar bone. This study used light microscopy to histologically and histometrically evaluate the effects of CSA on alveolar bone in the rat. METHODS: Sixty, 6-week-old Sprague-Dawley rats were separated into test and control groups. Animals in the test group received CSA in mineral oil (30 mg/kg body weight) daily by gastric feeding over the 6-week study. Control animals received only mineral oil. Ten animals from each group were sacrificed at weeks 2, 4, and 6. After histologic processing, the labial crest of the alveolar bone in the anterior mandible was evaluated by light microscopy. RESULTS: A distinct pattern of increased osteoclasia and reduced bone formation was observed in the CSA-exposed animals compared to the controls. Increased osteoclasia was observed in periodontal sites and decreased bone formation was observed in symphyseal sites. CONCLUSIONS: The results suggest that CSA has distinct effects on alveolar bone.

Alveolar Bone Loss↗

[Hypertrichosis and gingival hypertrophy regression in renal transplants following the substitution of cyclosporin by tacrolimus].

Gingival Hyperplasia (GH) and hypertrichosis (HT) are two sides effects associated with the usage of cyclosporine (CyA) but not with tacrolimus (FK 506). The aim of this study is to evaluate the efficacy and security of the conversion from CsA to FK 506 to treat those two complications. From August 1996 to May 1997, 15 patients (9 males, 6 females) aged from 23 to 63 years old (38 +/- 14, mean +/- SD) were switched from CsA to FK 506, 12 for GH, 2 for HT and one for combined presentation. FK 506 was first initiated at a dose of 0.15 mg/kg/day and then adjusted to a level target of 8 ng/ml. The conversion was done on an out patient basis at average 35 (5-83) months after transplantation. Patients were followed prospectively for 12 months. There was a significant reduction in GH in all patients within 3 months. Five out 13 patients had a complete resolution of GH within three months of conversion, 9/12 within 6 months and all by 12 months. HT resolved completely within 6 months. No rejection episode occurred and the serum creatinin remain stable over one year post conversion. Conversion from CsA to FK 506 is thus a safe and valid option to treat CsA induced GH and HT.

Adult↗

Candidate loci for Zimmermann-Laband syndrome at 3p14.3.

A male with 46,XY,t(3;17)(p14.3;q24.3) presented with gingival hyperplasia, hypertrichosis, unusually large ears and marked hypertrophy of the nose, characteristic of the Zimmermann-Laband syndrome (ZLS). Other features include large facial bones and mandibles, large protruding upper lip, enlarged fingers and toes, strabismus, and enlarged phallus. Knowledge of a 46,XX,t(3;8)(p21.2;q24.3) reported previously in a mother and daughter with ZLS suggests that the 3p14.3-p21.2 region may contain a gene responsible for ZLS. We have reassessed the chromosome 3 breakpoint region of the t(3;8) and revised its breakpoint location to 3p14.3, based upon an updated human genome sequence assembly. Using fluorescence in situ hybridization (FISH) with BAC clones, we have also identified a breakpoint spanning clone at 3p14.3 in our t(3;17) patient, thereby narrowing the breakpoint to a region of approximately 200 kb. These data suggest that the gene responsible for ZLS is located in 3p14.3 and implicates four likely candidate genes in this region: CACNA2D3, encoding a voltage-dependent calcium channel, LRTM1, a gene of unknown function embedded within CACNA2D3, WNT5A, encoding a secreted signaling protein of the WNT family, and ERC2, which codes for a synapse protein.

Adaptor Proteins, Signal Transducing↗

[Juvenile hyaline fibromatosis].

BACKGROUND: Juvenile hyaline fibromatosis is a sporadic hereditary disease with autosomal recessive mode of inheritance, characterized by the presence of nodules and tumours in the skin and soft tissues and gingival hyperplasia. The majority of patients are growth retarded, suffer from joint disorders, contractures, osteolytic lesions and have a positive family history. The disease most frequently occurs in children, but may be diagnosed also in adults. Since 1873, when the disease was described by Murray, only a few cases were introduced in the literature. THE AIM AND THE BASIS OF THE STUDY: To present the clinical pattern of a patient diagnosed at the age of 28 and describe also the histopathological, immunohistological and electron-microscopic findings of excisions from the lesions. METHODS: The pathologist in cooperation with clinicians from several disciplines established the diagnosis on the basis of typical histopathological picture and additional immunohistological tests not yet introduced in the literature. RESULTS: Tumorous lesions contained hyaline structureless matrix often with chondroid or even osteoid metaplasia, calcium salts. The matrix contained numerous fibroblastoid-like cells with eosinophilic cytoplasm, oval nucleus and often pericytoplasmic halo. ELMI investigation revealed dilated cisternae of rough endoplasmic reticulum and hypertrophic Golgi apparatus. Sporadically were particles with calcium salts density detected. Immunohistochemical tests revealed the expression of vimentin, alfa1-antitrypsin and alfa1-antichymotripsin by the tumorous cells. CONCLUSION: The authors presented an extremely sporadic childhood disease in an adult. The results are in accordance with the data from the literature. Immunohistochemical picture of "cementicles" has not yet been introduced in the available literature. (Fig. 12, Ref. 25.)

Adult↗

Telescope retainers for removable partial dentures.

Telescope crowns used in removable partial dentures reduce the destructive horizontal and rotational occlusal forces by directing them more axially and less traumatically than other retainers. These properties are combined with excellent conditions for cross-arch and multiple abutment splinting. If one of the abutment teeth needs extracting, the denture can be repaired with acrylic resin simply by remaking the secondary crown into a pontic. The single copings can be easily cleaned due to the good accessibility around their gingival margins. They also protect the abutment teeth against caries and thermal irritation. Soldering of the primary copings or their connection with accessory bars is not necessary in rigidly constructed telescope crowns, because the secondary crowns incorporated in the superstructure provide sufficient rigidity. In addition, the splinted copings often cause inflammatory gingival hyperplasia because the solder joints are too close to the gingival margins, preventing proper hygiene maintenance. The taper configuration or vertical height of the coping, which determines the amount of load transferred to each abutment, can be planned to suit each tooth's condition and designated function in the restoration. In a fixed restoration, the load is always evenly distributed among all the abutment teeth regardless of their condition, because the crowns are permanently cemented to them. In the telescope denture, the amount of force can be individually regulated by modifying the form and leverage of the retainers, according to their position, bone support, and occlusal conditions.

Crowns↗

Extensive papillomatosis of the palate exhibiting epithelial dysplasia and HPV 16 gene expression in a renal transplant recipient.

We report a unique case of extensive papillomatosis of the palate in a renal transplant recipient. The condition resembled inflammatory papillary hyperplasia; it exhibited severe epithelial dysplasia and concurred with generalized gingival hyperplasia. We document and discuss the probable multifactorial etiology of the lesions, including evidence for human papillomavirus (HPV) type 16 expression, as detected by in situ reverse transcription polymerase chain reaction. This report illustrates the need for careful clinical investigation and follow-up of immunosuppressed individuals presenting with apparently benign, common oral lesions.

Focal Epithelial Hyperplasia↗

Oral manifestations of acute myelomonocytic leukemia: a case report and review of the classification of leukemias.

BACKGROUND: Oral signs and symptoms may indicate a serious underlying systemic disease. The most frequently observed oral findings of leukemia are mucosal bleeding and ulceration, petechiae, and gingival hyperplasia. This case report describes a 53-year-old male who presented with gingival enlargement and bleeding, fatigue, and recent weight loss as initial manifestations of acute myelomonocytic leukemia. METHODS: A gingival biopsy was performed, revealing the presence of a hypercellular infiltrate of atypical myeloid and monocytic cells. Further work-up consisted of a complete blood count, bone marrow biopsy, and immunohistochemical and histochemical analysis of biopsy material and flow cytometry of peripheral blood. RESULTS: Flow cytometry results confirmed that the infiltrate was of a myelomonocytic origin, and a diagnosis of acute myelomonocytic leukemia was rendered. The patient responded well to a chemotherapeutic induction regimen of cytosine arabinoside and idarubicin hydrochloride, with regression of gingival enlargement and remission of disease. The patient continued with consolidation chemotherapy and an autologous bone marrow transplant, but eventually died 22 months after initial diagnosis. CONCLUSIONS: Oral health care professionals, especially periodontists, must recognize that gingival enlargement may represent an initial manifestation of an underlying systemic disease. Acute myelogenous leukemia is a hematological disorder with a predilection for gingival involvement.

Antineoplastic Combined Chemotherapy Protocols↗

The transmandibular implant: a 12-year follow-up study.

A clinical study of the use of the transmandibular implant for reconstructing severely atrophic mandibles in preparation for an implant-borne dental prosthesis was performed. This study included 368 patients with a mean height of the mandible of 10 mm. They were examined clinically, radiographically, and by means of a questionnaire. The follow-up period for 185 patients was 5 years or more, and 55 were followed 10 years or more. Removable dentures were made for 350 patients, and fixed bridges for 18 patients. Forty-three patients had reversible complications, one patient had unilateral hypesthesia, and six patients had their implant removed. Reversible complications of infection, gingival hyperplasia, loading of individual posts, and fenestration of threads were controlled without deleterious effects on the gingival, mucosal, or osseous tissues. The transmandibular implant system was shown to successfully restore function of the severely atrophic mandible in a more predictable manner than augmentation procedures.

Adult↗

Prevalence of periodontal disease in the primary dentition of children with cerebral palsy.

PURPOSE: The aim of this study was to evaluate periodontal disease prevalence in the primary dentition of children with cerebral palsy (CP). METHODS: The experimental group consisted of 100 children with CP and the control group, 100 healthy children. The indices used in the oral examination were: debris index, calculus index, gingival index, Simplified Oral Hygiene Index (OHI-S), and gingival hyperplasia index. RESULTS: It was observed that the mean values for the debris index, OHI-S, and gingival index were higher in the children with CP than in the control group. In the CP group, the percentage of children who had their teeth brushed by their parents or another person was greater than in the control group. CONCLUSIONS: From this study, it was concluded that children with CP had greater prevalence of periodontal disease in the primary dentition than children in the control group.

Anticonvulsants↗

[Behaviour of gingival elastic constituents in periodontal disease].

Disorganization of gingival elastic constituents, including oxytalan fibers, elaunin fibers, and mature elastic fibers, is a feature of periodontal disease. In early stages where inflammation is moderate, this disorganization involves only oxytalan fibers. As the disease progresses, fragmentation and lamination of elaunin fibers and mature elastic fibers is seen. In cyclosporin A-induced gingival hyperplasia, there is a substantial increase in the amount of elastic fibers as compared with healthy gingiva. In gingival fragments from edentulous elderly individuals the elastic constituents form dense granules.

Adult↗

Oral health in children with renal disease.

Thirty-eight children (aged 2-16 years) attending a regional kidney unit had a full clinical and radiological dental examination. Twenty had previously undergone a renal transplant, 11 had chronic renal failure and 7 had other renal diseases. Periodontal disease was uncommon The presence of gingival hyperplasia (gum overgrowth), as recorded in 22 of the children, did not show any relationship with the use of immunosuppressant therapy. However, gingival overgrowth was so excessive in 2 patients that surgical removal was required. The prevalence of dental caries was low. Enamel defects were common, and of an unusual pattern, with a much higher prevalence of diffuse opacities and enamel hypoplasia than in the normal child population, 83% and 22%, respectively. This increased prevalence is probably due to disordered calcium and phosphate metabolism. The prevalence of these defects may reflect an early onset of renal disease, since there were a number of very young children in the programme. Dental and medical care should be closely integrated for children with renal disease to avoid the undesirable dental sequelae of, in particular, gingival overgrowth, carcinoma and enamel hypoplasia.

Adolescent↗

Clinical evaluation of the efficacy and safety of an ultrasonic toothbrush system in an elderly patient population.

Twelve dentulous patients, age 65 or older, with no medical or pharmacologic predisposition to gingival hyperplasia were enrolled in a controlled clinical trial to evaluate the effect of the unaided use of an ultrasonic toothbrush on supragingival plaque and gingival bleeding. Subjects used the ultrasonic toothbrush in lieu of their conventional toothbrushes for a 30-day period. Clinical measurements of the plaque index and bleeding index were made at baseline and at 15 and 30 days. At the end of the 30-day test period, the use of the ultrasonic toothbrush resulted in significant reductions in plaque score, proportion of high plaque surfaces, and bleeding index. The reduction of the bleeding index indicates significant improvement in oral health. In this study, the use of an ultrasonic toothbrush effectively improved oral hygiene and health in a population that often exhibits decreased manual dexterity. Strategies for oral health care assessment and education for guidance of effective interventions are included in checklist form; level of care classification, cost in both time involved and equipment, and purchase instructions also are discussed. Further study is warranted to evaluate this instrument for cognitively impaired elders, who often rely on caregivers for oral hygiene procedures.

Aged↗

Human papillomavirus infection in cyclosporin-induced gingival overgrowth in renal allograft recipients.

BACKGROUND: Host immunity plays an important role in the development of human papillomavirus (HPV)-associated disease. The HPV infection in oral cyclosporin-induced gingival overgrowth in renal transplant recipients has not been investigated previously. The aim of this study was to establish the HPV infection of cyclosporin-induced gingival hyperplasia in renal transplant recipients through morphological changes and use of the in situ hybridization technique. METHODS: We examined 13 renal transplant recipient biopsies with gingival overgrowth lesions and 4 healthy mucosa samples of these patients. The histopathological diagnoses were established on the basis of widely accepted criteria, and the pathologist was not aware of the HPV result. An in situ molecular hybridization was carried out under low stringent conditions to detect HPV species with mixed biotin-labeled probes of HPV 6 and HPV 11, and under high stringent conditions with HPV 6, HPV 11, HPV 16, and HPV 18 probes for HPV typing. RESULTS: The HPV prevalence among the 13 samples studied was 92.31% (12/13), of which 4 tested positive for HPV 6-11 and 1 for HPV 16. The 4 biopsies of normal mucosa from gingival overgrowth patients were also reactive for HPV DNA. In 11/12 (91.7%) HPV-positive cases, koilocytotic atypia was found. CONCLUSIONS: The suppression of T-cell function by cyclosporin therapy can result in an increase of HPV infection, adding to the proliferative activity of cyclosporin in the oral mucosa.

Adolescent↗

Gingival response to removable orthodontic appliances.

The gingival state of twenty-four young patients (mean age 9.4 years) wearing simple removable orthodontic appliances was assessed and followed at monthly intervals for 4 months. In nine of the patients intentional relief of the appliance was made in the upper right area. The plaque index levels were similar in both "relief" and "no-relief" groups and reached a similar score at 4 months. The gingival index was significantly higher in the "relief" group, and all five cases that showed gingival hyperplasia of the experimental area belonged to this group.

Adolescent↗