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PubMed1989

Changes in release of N-acetyl-beta-D-glucosaminidase, gamma-glutamyl transpeptidase and leucine aminopeptidase from renal tubular cells under anaerobic conditions.

Although the urinary excretion of NAG and gamma-GTP has been employed as an index of renal injury, the mechanism of release of these enzymes remains unclear. In the case of renal ischemic injury, we recently reported that u-NAG and u-gamma-GTP were not correlated well with the degree of renal damage especially in the late phase of ischemic injury [1]. In order to elucidate the relationship between release of these enzymes and renal ischemic injury, we devised an in vitro model of anoxic injuries to renal tubular cells and measured the time-course release of NAG (a lysosomal enzyme), gamma-GTP and LAP (brush border enzymes) at 37 degrees C under five different aerobic and anaerobic conditions (95% O2, 20% O2, 10% O2, 3% O2 and 0% O2). The ATP and lactate levels in the system were also measured to estimate the metabolic state of the tubular cells. In the 3% O2, 10% O2 and 20% O2 groups, NAG, gamma-GTP and LAP were released into the media at a similar rate to that in the 95% O2 group, and anaerobic damage to the renal tubular cells was not observed. In the 0% O2 group, the NAG in the medium increased from 60 to 180 min at a significantly higher rate than that of the 95% O2 group, and a linear relationship was observed between NAG concentration and incubation time (r = 0.73), although NAG did not increase significantly at 30 min.(ABSTRACT TRUNCATED AT 250 WORDS)

Acetylglucosaminidase↗
PubMedDate not supplied

[Leucine aminopeptidase activity levels in various brain regions in the rat. Effect of the acute administration of lithium].

Lithium is widely accepted as an effective treatment in acute mania. However, the neurochemical mechanism of action of the ion still remains relatively obscure. In this work, the effect of acute lithium administration on Leu-aminopeptidase activity in discrete areas of the rat brain is described. Decreases in enzyme activity in the striatum and pituitary gland were observed. It is suggested that this enzyme plays a part in the mechanism of action of lithium through regulation of the activity of some neuroactive peptides.

Animals↗
PubMedDate not supplied

[Leucine aminopeptidase crystals from the bovine pancreas].

Crystals of leucinaminopeptidase from bovine pancreas were obtained. Space group (P6322), parameters of the cell a-b-132 A, c = 122 A and distribution of spot intensities on precessional X-ray patterns were in full agreement with corresponding parameters for leucinaminopeptidase crystals from bovine eye lens. A conclusion is drawn about similarity between the spatial structures of leucinaminopeptidase from bovine pancreas and eye lens.

Animals↗