PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “Thymus Extracts”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 649 records · Page 36Linked to original sources
PubMed1994

[Immunotherapy in the combined treatment of chronic destructive pulmonary tuberculosis].

The paper provides the results of treatment of chronic pulmonary tuberculosis patients by using immunoactive drugs (thymalin and T-activin) and prednisolone, which has been performed by monitoring immunological parameters. The patients with fibrous and cavernous tuberculosis display a combination of antituberculous antibody hyperproduction and specific cell-mediated response inhibition. Immunological hormone therapy allows normalization of the immunological status (an increase in the count and functional activity of T-lymphocytes) and relative stabilization of the process with cessation of bacterial isolation in shorter periods than in the controls, which is favourable to the reduction in hospital treatment periods.

Adjuvants, Immunologic↗
PubMed1993

Pharmacokinetic investigation with 125I labeled T-actemodulin (TAM).

The aim of the study was to label TAM with radioactive iodine and to follow its distribution in different organs of rats. T-actemodulin is a thymic hormone isolated from calf thymus by a new technology. TAM was labeled according to the method of Greenwood and Hunter. Two major peaks were observed on the gel chromatography: The first peak contained the labeled TAM; the second peak contained 125I which was not bound to the organic compound. Additional purification of the first peak was performed on a Sephadex G-75 column, and the eluate was designated as purified peak I. After a radioimmune testing it could be concluded that TAM was labeled successfully and the peak I contained 125I-TAM. Peak I, purified peak I and the peak II were applied to young male Wistar rats. The radioactivity of pancreas, kidney, spleen, adrenals, liver, thymus, bone marrow, testis and thyroid were measured after killing the animals 10 min, 60 min, and 24 h after the injections. The pharmacokinetic study showed that all organs, except for the thyroid have a higher affinity for labeled TAM than for the free 125I. Higher accumulation of labeled TAM was observed in the pancreas, kidney, adrenals and spleen. A specific uptake of labeled TAM in these organs can be suggested. Functional interrelations between thymus and pancreas, spleen, adrenals are discussed.

Animals↗
PubMedDate not supplied

[The formation of T-suppressors that suppress the activity of alloreactive T-cells after lymphocyte interaction with 2 different thymus-derived mediators].

The data are presented on simultaneous interaction of thymocytes and T-cells from lymph nodes with two humoral factors produced by macrophages and epithelial cells, main stromal elements of the thymus. Such contact of T-cells with two mediators resulted in the formation of T-suppressors capable of inhibiting the activity of syngeneic allospecific T-lymphocytes. These T-suppressors did not alter the response to another antigen but inhibited proliferation of donor cells. Involvement of such mechanism of T-suppressor formation in regulation of the immune status is discussed.

Animals↗
PubMedDate not supplied

[The role of thymic peptides in regulating ovarian morphogenesis].

Morphological analysis of the postnatal ovary development in mice treated with diverse T-activine doses during first 48 hours has shown that change of the thymic peptides content during neonatal period (single injection of the drug in doze 1,0-25,0 micrograms/l g body weight) results in negative ovariotropic effect, manifestations of which are doze-depending. In the case of administration of 5,0, 10,0, 25,0 micrograms/l kg of T-activine ovaritropic effect is limited with transitory disturbances of follicle genesis and therefore, with the delay of pubescence with the following (by the 10th month) reproductive function rehabilitation. After administration of T-activine 1 micrograms/l g in doze ovary dysgenesis with the regular reproductive disturbances was observed. Morphological and functional manifestations of the ovarian pathology in the case under discussion were similar with that of genetically non-thymus and neonatally thymus-ectomized animals, which, probably, may be considered to be the proof of the suggestion that thymic peptides dysbalanse on the early ontogenesis stages plays an important role in pathogenesis of the ovarian dysfunction in non-thymus animals.

Adjuvants, Immunologic↗
PubMed1996

[Evaluation of the effectiveness of immunomodulators in the treatment of patients with postoperative suppurative-septic complications].

The influence of immunoactive medicines on the cell immunity have been studied. There were differences in reaction to T-activin and Thymalin between various patients with septic postoperative complications. Thymalin stimulated T-cells while acting with immunoglobulins or immune plasmas only. T-activin led to the increase in the number of T-lymphocytes and B-lymphocytes independently of it's cooperation with the vaccines. The stimulating effect of myelopid demonstrated itself by increase in the number of B-lymphocytes and immunoglobulins M and G after 14 days of treatment. The positive clinical effect was more impressive when immunomodulators were used in combinations with the vaccines; still it did not correlate completely with the dynamics of the cell immunity parameters.

Activins↗
PubMed1996

[The histophysiological characteristics of ovarian development with an altered level of thymic peptides at infancy in mice].

Thymic peptides effect on the female gonad development was studied: consequences of these substances dysbalance in infancy (T-activine administration of 0.1 microgram/g of body weight in dose since 8 to 21 postnatal day) was analysed. Morphofunctional diagnostics of the ovarian state is based on the spontaneous organism reaction to the drug administration (to 21-days-old animals) and on the distant consequences of its activity (in 1-, 2-, 3-months female animals). Thymic peptides dysbalance in infancy promotes only transitory disorders of the ovarian histophysiology, expressing in transitory inhibition of the follicle genesis with the simultaneous atretic process activation, which results in puberty delay with the following complete restoration of reproductive function. Taking into account that in the early neonatal period gonad reacts to T-activine administration with the development of strong ovarian pathology, the results obtain allow to conclude that the reactivity to thymic peptides content oscillations reduces so as the significance of the factors cited in the ovarian morphogenesis regulation in proportion with the animals' maturity.

Adjuvants, Immunologic↗
PubMed1995

Identification of cells secreting a thymostimulin-like substance and examination of some histoenzymatic pathways in aging avian primary lymphatic organs: I. thymus.

Thymi of adult chickens (White Leghorn strain), ages of 6, 18, 45, days and 1, 3, 6, months, were studied by histological and histochemical stainings, histoenzymatic reactions (LDH, SDH, alpha-GPDH, NADH, NADPH, Ca++ dependent ATPase, pH 8.5) and by anti-thymostimulin immunoreaction. Positive reactions for thymostimulin-like substance, mucopolysaccharides and enzymatic activities were located in different epithelial compartments of the cortical and medullary zones. In 3 and 6 month-old chickens, there was a progressive reduction of the cortical area and of the number of cortical and medullary epithelial cells, and, in the thymi of the 6 month-old chickens, a partial decline in the immunological reactivity to anti-TS, but the intensity of the histochemical and enzymatic reactivities, in comparison with the thymi of the youngest chickens, was not decreased. This may suggest that the cortical and medullary epithelial cells still present in these thymi are capable of secreting and expressing activities and, thus, may maintain their functions.

Adjuvants, Immunologic↗
PubMed1996

Clinical significance of specific autoantibodies in juvenile dermatomyositis.

OBJECTIVE: To determine the prevalence and clinical association of myositis specific antibodies in an unselected group of patients with juvenile dermatomyositis (DM). METHODS: The sera of 42 subjects, representing an unselected group of patients from a single center, with juvenile DM and 7 others with idiopathic inflammatory myopathy (IIM) were examined for the presence of myositis specific antibodies by immunodiffusion against calf thymus extract and immunoprecipitation with HeLa extract. RESULTS: Of the subjects with juvenile DM, only 2 had evidence of antibodies specific to myositis (anti-Mi2). Three other patients with juvenile DM had defined autoantibodies not usually considered to be specific to myositis. Two of the 3 subjects had anti-PM-Scl; both developed features of scleroderma after the juvenile DM remitted. The 5 subjects with defined autoantibodies did not differ clinically from the remainder of the subjects with the exception of the late development of scleroderma features in 2. Fourteen other subjects with juvenile DM had unidentified bands on immunoprecipitation, which may represent as yet undiscovered myositis specific antibodies. No myositis specific antibodies were detected in any of the 7 subjects with other IIM syndromes. CONCLUSION: Based on our findings, we do not recommend routine clinical testing for these antibodies in children with typical juvenile DM. Further study of the unidentified bands seen in our subjects may lead to better understanding of the clinical groupings and etiopathogenesis of childhood myositis.

Adolescent↗
PubMedDate not supplied

[Immunological indicators in children with laryngeal papillomatosis and possible ways of immunocorrection].

Clinicoimmunological characteristics were followed up in the course of laryngeal papillomatosis (LP) in 96 children. It was found that they had suppressed T-cell immunity, phagocytosis which still worsen with the disease aggravation and recurrences. A decrease in nonspecific resistance, properdin, lysozyme, complement and secretory IgA indicated impairment of nasopharyngeal and laryngeal local immunity. A scheme of combined treatment is proposed incorporating basic therapy with reaferon, systemic immunomodulators (thymalin, T-activin) and local immunocorrection. Such approach reduced the time of basic treatment and prolonged remission 2.5-3-fold.

Adjuvants, Immunologic↗
PubMedDate not supplied

[The effect of tactivin and the intraduodenal administration of drug mixtures on the efficacy of the therapy of duodenal peptic ulcer].

Incorporated into the complex of therapeutic measures to treat duodenal ulcer associated with Helicobacter pylori (H. p.) were tactivin and intraduodenal administration of drug mixtures made up of pharmaco- and phytopreparations. Effectiveness of treatment was to be evidenced by healing of the ulcer defect, elimination of H. p. from the mucous membrane, and length of clinico-endoscopic remission. The greatest benefit from treatment occurred when tactivin was used in combination with intraduodenal administration a mixture of rotocan, olazol, proposol, methiluracil, and trychopol.

Adjuvants, Immunologic↗
PubMed1998

[The characteristics of the development of the immune function in the transplanted spleen of newborn mouse pups in recipients of different ages. 2. The effect of the thymus on the functional development of the transplant].

The effect of the neonatal thymus grafting or "Thymostimulin" administration on the cellularity, cell composition, immune, response to SRBC and proliferative activity of T- and B-cells in vitro were determined in neonatal spleen grafted CBA/Ca female mice of different ages. Analysis of the thymus graft effect on the T- and B-cells content in the spleen transplant from the adult and old recipients demonstrated no differences. The neonatal thymus grafting led to the essential increase of the immune response, spleen cellularity and to the diametrically opposed changing from negative to positive of the sign of the correlation coefficient between the T-cells content and the cellularity of the neonatal spleen in the old recipients. The similar effect of the neonatal thymus grafting was revealed in respect of correlative connection between content of the T-cells and PFCs in spleen grafted to the old recipients too. The "Thymostimulin" injection led only to the increase of the spleen transplant cellularity. These results suggests that the young thymic microenvironment is essential for the normal T-cells differentiation and for its normal function in the neonatal spleen transplant.

Adjuvants, Immunologic↗
PubMed1998

[Primary sclerosing cholangitis--tests with ubiquitin treatment. Case report].

Treatment of chronic liver disease, including primary sclerosing cholangitis (CSP) is a difficult and still not fully solved problem. Both monotherapy and combined pharmacological therapy have shown little effect in inhibiting the disease process and preventing complications. The objectives of ubiquitin biotherapy were restitution of the immune system and inhibition of the disease process along with the stimulation of regenerative processes of the liver. In 1994 in Gastroenterological Clinic attempts at ubiquitin biotherapy were made with the use of thymus extract (TFX-JELFA ini.), which proved to be the ubiquitin preparation that was not only active in the immune system but also played a significant role in regenerative and adjuvant processes. It has been shown that ubiquitins play an essential role in proteolysis of proteins, their intracellular elimination and in the apoptosis. The early results of the clinical observations and laboratory tests indicate a gradual improvement and inhibition of the disease process the patient with CSP.

Adult↗
PubMed1975

Thymosin activity in patients with cellular immunodeficiency.

An extract of calf thymus, thymosin, induces an increase in percentage of T-cell rosettes when incubated in vitro with sheep erythrocytes and lymphocytes from patients with primary immunodeficiency diseases or viral illness. Precursor lymphocytes are required for this increase to occur. The percentage of T-cell rosettes, when they are normal, is not increased further upon incubation with thymosin. A patient with thymic hypoplasia and immunoglobulin synthesis was selected to receive thymosin in vivo when her T-cell rosettes had increased from 15 to 48 per cent after in vitro incubation with thymosin. During therapy, she clinically improved, the percentage of T-cell rosettes gradually increased to normal, and positive delayed hypersensitivity skin tests developed. Thymosin may be useful clinically for partial reconstitution of cellular immunity. An increased percentage of T-cell rosettes after incubation with thymosin in vitro may predict which patients will respond to thymosin therapy in vivo.

Adult↗
PubMed1992

Isolation and characterization of a DNA helicase from cytosolic extracts of calf thymus.

A DNA helicase has been isolated from calf thymus tissue. The enzyme was enriched from crude cytosolic extracts by batchwise chromatography on phosphocellulose, followed by 35% ammonium sulfate precipitation, and subsequent chromatography on phenyl-Sepharose, single-stranded DNA cellulose, and AcA 44 gel filtration. The DNA helicase had a Stokes' radius of about 45 A and a sedimentation coefficient of 4.3 S. The most purified fractions contained three polypeptides with apparent molecular weights of 110, 65, and 34 kDa. UV crosslinking with radioactive dATP stained all three major polypeptides. The helicase catalyzed the unwinding of a DNA primer from a single-stranded DNA template in an ATP- or dATP-dependent manner. DNA unwinding was also observed with CTP or dCTP, but with reduced efficiency. The helicase translocated from 3' to 5' on the single-stranded template it was bound to. Relationships between this DNA helicase and other calf thymus helicases will be discussed.

Adenosine Triphosphate↗