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Ultrasonic measurement of enamel thickness: a tool for monitoring dental erosion?

OBJECTIVES: Wear of dental hard tissues, e.g. dental erosion, is reported to be a growing problem. A non-destructive measurement of enamel layer thickness would provide the opportunity for both early diagnosis, and longitudinal measurement of progressive enamel loss. It was the aim of this study to investigate the potential of ultrasonic pulse-echo measurements for the enamel thickness measurement. METHODS: Nine extracted human incisor teeth were selected and stored in physiological saline. Mesial and distal tooth parts were removed, resulting in a central tooth slice of about 2 mm thickness. Where possible three buccal, and one palatal measuring sites were selected and indicated by pencil marks on one of the section planes. Ultrasonic pulse-echo measurements were made at each site using a Panametrics 25DL thickness gauge (Panametrics, Waltham, MA, USA), using a perspex delay line transducer (15 MHz) and glycerine coupling medium. Ultrasonic measurements were validated by measuring the thickness of the enamel layer at the marked side of the tooth slices with a light stereomicroscope at 120 x magnification. Two observers performed independent measurements. RESULTS: Limits of agreement for measurements by two observers (n = 42) were -0.09 and 0.09 mm. Measurements performed at 21 degrees C and 34 degrees C were not significantly different, as analysed by paired Student's t-test (p = 0.19). Pearson's correlation coefficient between ultrasonic and microscopic measurements was 0.90. Analysis of all measurements from both observers at both temperatures yielded a sound velocity in enamel of 6.5 x 10(3) m/s (standard error 0.1 x 10(3) m/s). CONCLUSIONS: It was concluded that the ultrasonic measurement of the enamel thickness is feasible without enamel preparation.

Dental Enamel↗

The laboratory assessment of enamel erosion: a review.

OBJECTIVES: To review the various methods and techniques available to assess enamel erosion in vitro. DATA: Peer reviewed scientific articles. SOURCES: Medline and Web of Science searches and manual searching. STUDY SELECTION: Laboratory based assessments only included. CONCLUSIONS: A number of macroscopic and microscopic techniques have been used to assess enamel erosion in vitro and in situ. This review examines techniques which are either well established or comparatively novel techniques that are being explored for their potential.

Dental Enamel↗

Immediate erosive potential of cola drinks and orange juices.

Little is known about the erosive potential of soft drinks within the first minutes of exposure to teeth, and about the potentially protective role of salivary proteins. We hypothesized that the erosive potential is determined primarily by pH and decreases in the presence of salivary proteins. To investigate this, we first added uncoated hydroxyapatite crystals and, second, salivary-protein-coated hydroxyapatite crystals to 20 commercially available cola drinks and orange juices simultaneously, with pH recordings every 15 sec for 3 min. The amount of apatite lost per liter of soft drink per sec was calculated from titratable acidity values to each pH obtained by crystal addition. The erosive potential within the first minutes of exposure was determined solely by the pH of the drink, and the erosive potential was ten-fold higher in cola drinks compared with juices. However, salivary proteins reduced the erosive potential of cola drinks by up to 50%.

Beverages↗

The degree of enamel erosion by five different kinds of fruit.

It could be expected that the degree of enamel erosion by different fruits containing different kinds of organic acids, or different ratios of acids, and other different chemical components, will vary. The aim of this study was to investigate the degree of enamel erosion by different kinds of fruits (by determining the depths of enamel removed at different stages) over a period of 40 minutes. Statistically significant differences (p less than 0.05) were found among the slopes of apricot and that of grape, guava, apple, and orange. The results demonstrated that the degree of enamel erosion (etch depth) over a six-minute period had the following sequence: apricot more than grape and guava more than apple and orange; while over a 40-minute period the degree of etch depth was grape greater than apricot greater than guava greater than apple greater than orange. The degree of enamel erosion initiated by a fruit juice, as marketed, was about 5-8 times higher than that of the fruit (minced fruit juice). It also became clear that the degree of enamel erosion by different fruits depended on a combination of factors, such as the pH, amounts, and ratios, as well as the types of organic acids and other chemical components present in the fruits.

Citrus↗

Human enamel dissolution in citric acid as a function of pH in the range 2.30< or =pH< or =6.30--a nanoindentation study.

The objective of this study was to investigate the dissolution of human enamel in citric acid solutions over a wide range of pH. The in vitro conditions are considered to be relevant to soft drink-induced enamel erosion. Nanoindentation was used to investigate changes in the nanomechanical properties of polished enamel surfaces after exposure to citric acid solutions. Solutions used had 38.1 mmol l-1 citric acid and pH greater than 2.3 but less than 6.3 (2.30 < or = pH < or = 6.30). Samples were exposed to rapidly stirred, constant composition solutions for 120 s. Statistically significant changes in enamel hardness and reduced elastic modulus were observed after exposure to all solutions. There was an approximately linear dependence of enamel hardness on solution pH for 2.90 < or = pH < or = 6.30. Below pH 2.90, enamel is thought to have reached the lowest possible hardness value. The reduction in enamel dissolution caused by an increase in pH of a soft drink is likely to be small. Product modification to reduce the erosive potential of drinks may require additional methods such as addition of calcium salts.

Citric Acid↗

Geographic comparisons of the incidence of dental erosion: a two centre study.

This study revealed that the premolars in both arches are most commonly afflicted with erosion. The same results were obtained in an earlier study conducted by Sognnaes, Wolcott & Xhonga (1972) and Xhonga, Wolcott & Sognnaes (1972) evaluating the erosion pattern on extracted teeth. These findings should be helpful in further investigation into the aetiology and treatment. Educational, epidemiological and preventive concerns derive from a study establishing the dental erosion frequency on a large population sample (approximately 600 individuals and 1500 teeth). The comparative results for both locations used will provide a more significant basis for future studies of a similar nature.

Adolescent↗

Occupational exposure to acidic chemicals and occupational dental erosion.

OBJECTIVE: This study investigated the association between occupational acidic chemicals (ACs) exposure and occupational dental erosion. METHODS: A cross-sectional study was conducted in which three dentists surveyed 951 subjects from 42 factories using five types of ACs below Korean Threshold Limit Values (K-TLVs). Subjects agreed to participate by a written consent; 519 were acid exposed workers and 431 were non-exposed. The modified ten Bruggen Cate's criteria was used to classify erosion. Length and type of exposure to ACs were assessed using questionnaires. Logistic regression analysis including interaction terms was applied. RESULTS: ACs exposure was associated with erosion severity. Multiple exposures to ACs were found to be strongly associated with severe erosion. Interaction between wearing masks and AC exposure was significant. CONCLUSIONS: This study showed a clear association between AC exposure below K-TLVs and erosion. Hence, the authors propose to lower K-TLVs of five types of ACs.

Acids↗

Caries trends 1996-2002 among 6- and 12-year-old children and erosive wear prevalence among 12-year-old children in The Hague.

In 2002 a dental survey amongst 6- and 12-year-old schoolchildren (n = 832) in The Hague was carried out. The caries findings were compared with findings from earlier studies in The Hague. Caries prevalence (% of caries-free children) and caries experience (mean dmfs scores) among 6-year-old children had not changed significantly in the period 1996-2002. However, a significant increase of caries-free 12-year-old children of low socio-economic status was found in the period 1996-2002. The proportions of caries-free 12-year-old Dutch, Turkish and Moroccan children of low socio-economic status were 88, 69 and 78%, respectively, in 2002. The average DMFT score of 12-year-olds reached a minimum of 0.2. In 2002, 24% of the 12-year-olds exhibited signs of erosion, indicating that the presence of erosive wear was high among youngsters in The Hague.

Analysis of Variance↗

Oral signs and symptoms in the diagnosis of bulimia.

Bulimia is an eating disorder disease that presents difficulties in its diagnosis, primarily because of the secretive nature of the person with the disease. The disease is most common in young females, and there is an average of a 4-year delay between the onset of the disease and the patient's self-referral for treatment. Objective oral signs and symptoms of bulimia do exist and their presence can facilitate a diagnosis of bulimia during routine examination. The realization that five oral signs and symptoms of bulimia--enamel erosion, salivary gland enlargement, xerostomia, oral mucosal erythema, and cheilosis--are associated with the disease can eliminate some laboratory tests as well as facilitate an earlier diagnosis of bulimia.

Adolescent↗

[Studies on the causative factors of cervical wedge-shaped defect of human teeth from three dimensions].

OBJECTIVE: To investigate causative factors of cervical wedge-shaped defect of human teeth and the significance of methods for measurement of the defect. METHODS: Experimental cervical defects on 9 pairs of premolars were created by cross-brushing, erosion, or fatigue of occlusion force separately or combinedly and measured in depth and area of profile as well as in volume. RESULTS: 1. Profile depth of defects caused by three factors separately showed no statistical difference, but the profile depth caused by combining factors was much more than that caused by each individual factor; 2. The hardness of enamel and dentin in defect area was lower than that in normal area of tooth samples; 3. Measurement from one-dimension (depth) and two-dimension (area) showed only low relation in profile depth, however, measured three-dimensionally (volume) there existed moderate relationship. CONCLUSION: For study of cervical defect, the profile depth is feasible provided that is a large sample group and without equipment for volume measuring; if the sample size is limited and there is sophiscated instrument for volume measuring, the 3-dimensional measurement is the most sensitive one.

Adolescent↗

Wear in dentistry--current terminology.

There is some confusion in the dental literature concerning the phenomenon of wear which is usually considered in terms of its clinical manifestations rather than its underlying processes. The parent discipline for the study of these processes is tribology. The terms attrition, abrasion and erosion are common to both dentistry and tribology; however their meanings are different. Even within the various disciplines of dentistry there are differences in the understanding of the same terms. This article relates the dental appreciation of wear to the underlying processes.

Humans↗

Human enamel erosion in constant composition citric acid solutions as a function of degree of saturation with respect to hydroxyapatite.

The objective of this study was to investigate human enamel erosion under constant composition conditions, as a function of solution degree of saturation (DS) with respect to hydroxyapatite. The experimental conditions were relevant to the initial stages of enamel erosion by soft drinks. Nanoindentation was used to compare enamel surface softening caused by a control mineral water and two citric acid solutions with DS = 0.000 and DS = 0.032, both having pH 3.30. Enamel hardness and reduced elastic modulus were measured after 0, 30, 60, 120, 300 and 600 s exposure. A statistically significant change in enamel hardness was detected after 30 s exposure to both citric acid solutions, indicating that nanoindentation is extremely sensitive to the initial stages of erosion. There was a statistically significant difference between the mechanical properties of enamel exposed to the two citric acid solutions after 30, 60 and 120 s. At these times, the solution with DS = 0.000 caused twice as much enamel softening as that with DS = 0.032. This demonstrates that it may be possible to design a soft drink with a low erosive potential and a good taste by a small change in DS, at a typical drink pH.

Beverages↗

Experimental erosion of dentin.

Experimental erosion in human dentin was investigated with scanning electron microscopy. Erosion was caused by solutions containing either 0.030 mekV/g malic acid (pH 3.4), 0.034 mekV/g phosphoric acid (pH 2.6), or 0.038 mekV/g citric acid (pH 2.8). Test specimens prepared from the coronal parts of erupted third molars were immersed in the solutions from 30 s to 60 min. When compared with controls, exposure of dentin tubules was observed in all the specimens already after 30 s immersion, irrespective of the acid used. Mineral loss was seen to progress at the interface between peritubular and intertubular dentin in specimens immersed for 60 s in the acidic solutions. Longer times of immersion resulted in hollowing of the tubular openings by complete destruction of the peritubular dentin. Surface roughness and porosities were seen also at the intertubular areas. Although based on a simplified study model, our results may explain why so many patients with erosions suffer from painful sensitivity of their teeth: the acids causing erosion can expose inner dentin structures to outer stimuli by significant enlargement of the dentinal tubules.

Acid Etching, Dental↗

Erosive effect of a new sports drink on dental enamel during exercise.

PURPOSE: To compare the potential erosive effect of a prototype carbohydrate-electrolyte drink (PCE) with a neutral control (water) and a commercially available carbohydrate-electrolyte drink (CCE) during exercise. METHODS: : Nineteen healthy adults (male, N = 16; female, N = 3) took part in this single blind, three-way crossover study. Subjects were given each of the three drinks according to a randomization schedule, approximately balanced for first-order carryover effects. At the beginning of each of the three study periods, the volunteers were fitted with an intraoral appliance containing two human enamel blocks. During each study period, volunteers exercised for 75 min.d(-1) (5 repetitions of 15 min of exercise, with 5-min rests between exercise repetitions), 5 d.wk(-1) for 3 wk. Each day, drink aliquots of 200 mL were consumed during a 5-min period before exercise and after every 15-min bout of exercise, followed by a final 400-mL aliquot ingested over a 10-min period: a total of 1400 mL per study day. Dental erosion was measured as tissue loss from the enamel blocks by profilometry at the end of each study period. RESULTS: Water, PCE, and CCE produced 0.138 microm (SD 0.090 microm), 0.138 microm (SD 0.038 microm), and 4.238 microm (SD 3.872 microm) of enamel loss, respectively. A Wilcoxon t statistic showed a significant statistical difference between the PCE and CCE drinks (P < 0.001), whereas no significant difference could be detected between the PCE drink and water (P = 0.740). CONCLUSION: The PCE solution showed minimal erosion compared with the commercially available drink, and was statistically indistinguishable from water under the conditions of this study. Use of CE solutions formulated to minimize erosion during exercise may provide significant dental benefits.

Adult↗

[Influence of teas with fruit aroma and ice teas on pH and buffer capacity of saliva].

A number of teas with fruit aroma and ice teas has been tested on erosivity. The teas with a fruit taste have at their drinking temperature (45 degrees C) a pH between 6.2 to 7.4. Even teas with a citron or citrus flavour are neutral. The most acidic tea tested has a mango-peach-taste, but contains no buffer system. After mixing 1 ml of total saliva up to 10 ml tea or by rinsing the oral cavity with tea the pH of oral fluid was hardly lowered (minimum 6.3). In contrast, ice teas are rather acidic (pH < 4) and have a strong buffer capacity. Rinsing the oral cavity with 5 ml ice tea resulted in a decrease of the pH of the oral fluid to 4.1-4.9. However, within 2 minutes the pH in the oral fluid was already increased to 5.9-7.1 and after 5 minutes to 6.3-7.1. In other words, the teas with fruit aroma, based on organic taste components do not have an erosive potential. On the other hand, ice teas decrease the pH of the oral fluid drastically to a level that they become erosive. Therefore, from a dental point of view, teas with only a taste of fruit can be advised to replace drinking of soft drinks, but ice teas not.

Beverages↗

Erosion of dental enamel among competitive swimmers at a gas-chlorinated swimming pool.

In September 1982, two Charlottesville, Virginia, residents were found by their dentists to have general erosion of dental enamel consistent with exposure to acid. Both patients were competitive swimmers at the same private club pool. No other common exposure could be determined. An epidemiologic survey was made of 747 club members. Symptoms compatible with dental enamel erosion were reported by 3% of nonswimmers (9/295), 12% of swimmers who were not members of the swim team (46/393), and 39% of swim team members (23/59). All four swimmers with clinically verified dental enamel erosion had trained regularly in the pool for competitive swimming meets, compared with one of eight matched swimmers without enamel erosion. Examination of the implicated swimming pool revealed a gas-chlorinated pool with corrosion of metal fixtures and etching of cement exposed to the pool water. A pool water sample had a pH of 2.7, i.e., an acid concentration approximately 100,000 times that recommended for swimming pools (pH 7.2-8.0). A review of pool management practices revealed inadequate monitoring of pool water pH. Acid erosion of dental enamel--"swimmer's erosion"--is a painful, costly, irreversible condition which can be caused by inadequately maintained gas-chlorinated swimming pools.

Adolescent↗

The prevalence of dental erosion in the maxillary incisors of 14-year-old schoolchildren living in Tower Hamlets and Hackney, London, UK.

The purpose of this study was to assess the prevalence of dental erosion in the maxillary incisors of a sample of 14-year-old schoolchildren and to explore the aetiological factors responsible for that erosion. The cross-sectionally design study took place in secondary schools in inner-city London, UK and involved 525, 14-year-old schoolchildren selected at random in a clinical examination and a self-completed questionnaire. The outcomes measures for dental erosion were; the prevalence, the area and depth of lesions and the risk factors. The prevalence of labial and palatal erosion was 16.9 per cent and 12 per cent respectively. Risk factors and behaviours including daily frequency of ingestion of acidic fruits and drinks, food vomiting, toothbrushing frequency, and swimming habits were not shown to have any relationship with the presence of erosion. It was concluded that the prevalence of erosion in the maxillary incisors of this sample was higher labially and lower palatally than in previously reported national figures. The risk factors which were investigated were not shown to have any relationship with the presence of erosion. Further investigations of these issues are necessary to establish whether or not dental erosion is a public health problem in the UK.

Acids↗