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At least 667 records · Page 37Linked to original sources
PubMed2000

Pulpal reactions to two experimental bonding systems for pulp capping procedures.

This study evaluated the pulpal responses induced by application of two types of bonding system to the exposed dental pulp. One consisted of the following steps: etching with neutralized EDTA, application of an experimental water-based photocuring bonding agent, and restoration with a commercially available photocuring resin composite (EDTA etching system). The other was treatment with an experimental water-based self-etching primer, application of a commercially available bonding agent, and restoration with a commercially available photocuring resin composite (self-etching system). These two systems of treatment were applied to the exposed pulp. Calcium hydroxide was used as a control for the direct pulp-capping material. The pulps in class V cavities in the anterior teeth of beagles were mechanically exposed and then filled using the etching, the self-etching, or the Ca(OH)2 system. The beagles were sacrificed on the 7th, 30th or 90th postoperative day, and pulpal responses were investigated histopathologically using light microscopy. The EDTA etching system induced severe pulp reactions at 7 days after the operation. These reactions did not completely diminish after 90 days. Reparative dentin formation was observed at day 90. The self-etching system showed moderate pulp reactions, which gradually decreased over the experimental period. Reparative dentin bridge formation was observed at day 90. No necrosis of the pulp was observed at any time. Calcium hydroxide induced both moderate and severe initial pulp reactions, with reparative dentin formation evident at day 30. Necrosis was observed in the superficial pulp. It is suggested that the EDTA etching agent caused not only pulpal damage but also re-bleeding because of rinsing and drying. The self-etching system is a promising system for direct pulp capping.

Acid Etching, Dental↗
PubMed2005

The comparison of a dentin adhesive with calcium hydroxide as a pulp-capping agent on the exposed pulps of human and sheep teeth.

OBJECTIVE: This study evaluated the biocompatibility of a one-step dentin bonding agent (Prime&Bond 2.1) in pulp capping compared with calcium hydroxide [Ca(OH)2]. METHOD AND MATERIALS: Thirty sheep teeth and 20 intact human premolars were used. After cavity preparation, pulp exposure was achieved with a bur (#390). Adhesive pulp capping was performed in 25 teeth (15 sheep and 10 human). In the control group (12 sheep and 10 human teeth), pulps were capped with Ca(OH)2 and all of the cavities in both groups were sealed with resin composite. Three of the sheep teeth were used as intact controls. Teeth were extracted 7 or 90 days following treatment and prepared for histological examination and bacterial detection. RESULTS: At 7 days, severe inflammatory responses underlying the bonding agent and in the coronal pulp were observed with soft tissue disorganization in both human and sheep teeth capped with Prime&Bond 2.1. All of the teeth capped with Ca(OH)2 exhibited mild inflammatory reactions limited with the perforation area. After 90 days with the bonding agent, in 3 of 9 sheep teeth, chronic inflammatory reactions were significant, while slight pulpal reactions were observed in the others and dentin bridge formation in all of the sheep teeth was found. However, in human pulps, persistent, unresolved inflammation with the lack of dentin bridge formation was observed. In the Ca(OH)2 group, pulp repair with dentin bridging was found in all of the teeth, both sheep and human. No correlation was found between the presence of inflammation and bacterial staining using Spearman rank correlation test (P > .05). CONCLUSION: Prime&Bond 2.1 facilitates enhanced pulp healing and bridge formation in sheep teeth, but in human teeth it was not as successful as Ca(OH)2 as a pulp capping agent.

Acetone↗
PubMed2000

Immunohistochemistry of bone sialoprotein and osteopontin during reparative dentinogenesis in vivo.

OBJECTIVE: To investigate the stage-specific and tissue expression of bone sialoprotein (BSP) and osteopontin (OPN) during reparative dentinogenesis in vivo. METHODS: Direct pulp-capping with Ca(OH)2 developed a model used for the investigation of reparative dentinogenesis in the exposed dental pulp. In this model, standardized class V cavities were prepared close to the gingival margin on the buccal surface of each tooth. Animals were sacrificed 3, 7, and 10 days post-operation. Immunohistochemical staining determined the tissue-specific expression of BSP and OPN during the process. RESULTS: Odontoblast-like cells and reparative dentin reacted positively with BSP and OPN. The expression of BSP reached a peak at day 7, then gradually decreased, while the expression of OPN showed no significant difference between day 7 and day 10. CONCLUSION: BSP and OPN may play different roles in reparative dentinogenesis. BSP may serve as a nucleator of hydroxyapatite crystal formation.

Animals↗