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Influence of aerobic fitness level on measured and estimated perceived exertion during exhausting runs.

The purpose of the present investigation was 1) to study the effects of fitness level on perceived exertion (RPE) and estimated time limit (ETL) scales during exhausting runs, and 2) to predict time to exhaustion from RPE or ETL values collected during a constant run exercise. Eight high-fitness level and twelve moderate-fitness level endurance trained males performed two exhausting exercises on a 400-m running track. The first test was a graded exercise using a portable metabolic system to determine maximal oxygen uptake (V.O (2)max), the velocity associated with V.O (2)max (vV.O (2)max), the velocity at the lactate threshold (vLT) and the velocity at delta 50 (vDelta50 : the velocity halfway between vV.O (2)max and vLT). The second test was a constant run exercise at vDelta50 to determine the time to exhaustion at this intensity (tlimvDelta50). Moderate-fitness level athletes perceived exercise to be relatively more strenuous and felt that they could continue for less time than high-fitness level athletes at similar relative velocities. There was no effect of fitness level on perceived exertion for a given relative exercise duration. RPE corresponding to vLT was not statistically significantly different between the two levels groups. For the two groups, measured and predicted exhaustion time values, which were calculated from linear extrapolation of RPE and ETL values collected during the first 4 minutes of a submaximal constant run exercise, were not statistically significantly correlated. These results indicate that the aerobic fitness level seems to influence perceived exertion only during graded exercise. Consequently, if RPE is used to prescribe an exercise intensity, the prescription must be individualised regarding the aerobic fitness level of the athlete except for exercise intensities corresponding to vLT. Moreover, the perceived exertion pattern at the beginning of a submaximal constant run exercise could not be considered as a sensitive predictor of the point of self-imposed exhaustion whatever the fitness level of the athletes.

Adult↗

Effects of downhill treadmill running on uncoupling protein 3 mRNA expression.

Eccentric biased exercise has been reported to elicit more muscle injury than concentric or isometric exercise and potentially generate increased oxidative stress one to two days post exercise. Increased oxidative stress has been shown to up-regulate the expression of UCP3 mRNA. The aim of this study was to investigate the effects of downhill running on skeletal muscle UCP3 mRNA expression. Twenty-four male Sprague Dawley rats were randomly assigned to run continuously for 30 minutes (30-C, n = 6), or run six 5-minute bouts separated by rest periods of 2 minutes (2-R, n = 6), 4 minutes (4-R, n = 6), and 6 minutes (6-R, n = 6) on a 16 degree declined treadmill at a speed of 16 m. min (-1). Sham control animals (n = 8) were placed in a treadmill chamber during the 30-minute run session. Semi-quantitative RT-PCR was conducted to evaluate UCP3 mRNA levels in the plantaris, a muscle used eccentrically during downhill running and tibialis anterior, a muscle which undergoes very little eccentric muscle contraction during this exercise. The level of gene expression was normalized to 18 S ribosomal mRNA expression from the same PCR product. Results are reported as mean +/- standard error. UCP3 of the plantaris muscles from 2-R animals (2.36 +/- 0.13) was significantly greater than UCP3 of the plantaris from control animals (1.72 +/- 0.13), p < 0.05. UCP3 of the tibialis anterior from the continuous group (1.51 +/- 0.17) was significantly less than the UCP3 of the tibialis anterior of the control group (2.09 +/- 1.4), p < 0.05. These data suggest that downhill treadmill running is associated with an increase in UCP3 mRNA expression in the plantaris muscle. These results indicate that exercise which is biased toward eccentric exercise may up-regulate UCP3 mRNA during the period post exercise when muscle damage and repair is elevated.

Animals↗

The effects of muscle damage on running economy in healthy males.

Published information on aspects related to muscle damage and running economy is both limited and contradictory. To contribute to the current debate, we investigated the effects of an eccentric exercise session on selected muscle damage indices in relation to running economy using 10 (mean age 23 +/- 1 years) healthy male volunteers. The eccentric exercise session consisted of 120 (12 x 10) maximal voluntary repetitions by each randomly selected leg at the angular velocity of 1.05 rad . s (-1). Muscle damage (creatine kinase, delayed onset muscle soreness, range of movement, and eccentric, concentric and isometric [at 60 degrees and 110 degrees knee flexion] peak torque) and running economy (oxygen consumption, pulmonary ventilation, respiratory exchange ratio and breaths per minute during treadmill running at 133 and 200 m . min (-1)) indicators, were assessed pre-, 24-, 48-, 72- as well as 96-h after exercise. All muscle damage indicators revealed significant changes at almost all time-points of assessment compared to pre-exercise data (p < 0.05). However, none of the running economy parameters disclosed any significant change throughout the study (p > 0.05). It was concluded that changes in muscle damage and muscle performance as measured in this study are not reflected by concomitant alterations in running economy at submaximal intensities.

Adult↗

[Osteoarthritis from long-distance running?].

Long distance running has become a fashionable recreational activity. This study investigated the effects of external impact loading on bone and cartilage introduced by performing a marathon race. Seven beginners were compared to six experienced recreational long distance runners and two professional athletes. All participants underwent magnetic resonance imaging of the hip and knee before and after a marathon run. Coronal T1 weighted and STIR sequences were used. The pre MRI served as a baseline investigation and monitored the training effect. All athletes demonstrated normal findings in the pre run scan. All but one athlete in the beginner group demonstrated joint effusions after the race. The experienced and professional runners failed to demonstrate pathology in the post run scans. Recreational and professional long distance runners tolerate high impact forces well. Beginners demonstrate significant changes on the post run scans. Whether those findings are a result of inadequate training (miles and duration) warrant further studies. We conclude that adequate endurance training results in adaptation mechanisms that allow the athlete to compensate for the stresses introduced by long distance running and do not predispose to the onset of osteoarthritis. Significant malalignment of the lower extremity may cause increased focal loading of joint and cartilage.

Adult↗

Physiological determinants of time to exhaustion during intermittent treadmill running at vV(.-)O(2max).

Previous studies have reported large between-subject variations in the time to exhaustion during intermittent running at the velocity at V(.-)O (2max) (vV(.-)O (2max)). This study aimed to determine which physiological factors contribute to this variability. Thirteen male runners (age 38.9 +/- 8.7 years) each completed five treadmill running tests; two incremental tests to determine V(.-)O (2max), vV(.-)O (2max), the lactate threshold velocity (vLT) and the running velocity--V(.-)O (2) relationship; the third test to determine the time to exhaustion during continuous running at vV(.-)O (2max) (t (lim)cont); the fourth to determine the maximal accumulated oxygen deficit (MAOD); the fifth to determine the time to exhaustion during intermittent running at vV(.-)O (2max) (t (lim)int). Relief intervals during the intermittent test were run at 70 % vV(.-)O (2max). The vLT-vV(.-)O (2max) difference was significantly correlated with t (lim)int (r = - 0.70; p = 0.007). The correlation coefficient increased to r = - 0.83 (p < 0.001) when the difference between the relief interval velocity and the vLT was deducted from the vLT-vV(.-)O (2max) difference (theoretically representing the net depletion of the MAOD during each work/relief interval cycle). The main finding of this study was that 49 % of the variance in t (lim)int was explained by the vLT-vV(.-)O (2max) difference, compared to 74 % for t (lim)cont. However, a further 20 % of unique variance in t (lim)int could be explained with the inclusion of the relief interval velocity-vLT difference. Theoretically, runners with the largest relief interval velocity-vLT difference will replete their anaerobic capacity to a greater extent during each relief interval, thereby increasing time to exhaustion.

Adult↗

Ventilatory thresholds assessment from heart rate variability during an incremental exhaustive running test.

The present study examined whether the ventilatory thresholds during an incremental exhaustive running test could be determined using heart rate variability (HRV) analysis. Beat-to-beat RR interval, V(.-)O (2), V(.-)CO (2) and V(.-) (E) of twelve professional soccer players were collected during an incremental test performed on a track until exhaustion. The "smoothed pseudo Wigner-Ville distribution" (SPWVD) time-frequency analysis method was applied to the RR time series to compute the usual HRV components vs. running speed stages. The ventilatory equivalent method was used to assess the ventilatory thresholds (VT1 and VT2) from respiratory components. In addition, ventilatory thresholds were assessed from the instantaneous components of respiratory sinus arrhythmia (RSA) by two different methods: 1) from the high frequency peak of HRV ( FHF), and 2) from the product of the spectral power contained within the high frequency band (0.15 Hz to fmax) by FHF (HF x FHF) giving two thresholds: HFT1 and HFT2. Since the relationship between FHF and running speed was linear for all subjects, the VTs could not be determined from FHF. No significant differences were found between respective running speeds at VT1 vs. HFT1 (9.83 +/- 1.12 vs. 10.08 +/- 1.29 km x h (-1), n.s.) nor between the respective running speeds at VT2 vs. HFT2 (12.55 +/- 1.31 vs. 12.58 +/- 1.33 km x h (-1), n.s.). Linear regression analysis showed a strong correlation between VT1 vs. HFT1 (R (2) = 0.94, p < 0.001) and VT2 vs. HFT2 (R (2) = 0.96, p < 0.001). The Bland-Altman plot analysis reveals that the assessment from RSA gives an accurate estimation of the VTs, with HF x FHF providing a reliable index for the ventilatory thresholds detection. This study has shown that VTs could be assessed during an incremental running test performed on a track using a simple beat-to-beat heart rate monitor, which is less expensive and complex than the classical respiratory measurement devices.

Adult↗

[Grading of the functional sport shoe parameter "cushioning" and "forefoot flexibility" on running shoes].

On nearly every running event a heterogeneous structure of participants regarding body height and body weight can be observed. This study should figure out whether the running shoe manufacturers will consider this anthropometric variability. Moreover it should be investigated the runners needs based on different anthropometrics regarding preferred cushioning and forefoot flexibility properties. In order to check whether the running shoe manufacturers will apply a grading pattern, a dynamic material study with conventional running shoes in different sizes was conducted. In a second step a field study in Middle Europe and North America with 244 female and 227 male runners was organized. Every subject had to run and evaluate 7 different shoe modifications. Based on the material study it is to state, that the running shoe manufacturers currently do not consider a systematic grading of cushioning and forefoot flexibility properties. In contrast to this, the field study reveals the necessity to grade these properties. A shoe size dependent and a geographic grading concept are suggested. It is supposed, that the application of these grading concepts do not only provide a comfort improvement, but they also contribute to a reduction of joint loads of the lower extremities and consequently to a prevention of overuse injuries.

Adult↗

The accuracy of the critical velocity test for predicting time to exhaustion during treadmill running.

The purpose of this investigation was to determine the accuracy of the critical velocity (CV) test for predicting time to exhaustion (time limit = TL) during treadmill running. Ten adult males (mean +/- SD of age = 23 +/- 2 years) volunteered to perform a maximal treadmill test, a CV test, and five exhaustive treadmill runs at 70%, 85%, 100%, 115% and 130% of CV for the determination of actual TL. Related t-tests revealed significant (p less than 0.05) differences between the predicted and actual TL values for velocities equal to 100 and 130% of CV. The correlations between predicted and actual TL values for velocities above CV ranged from r = 0.957 to 0.980 (SEE = 0.28-0.82 minutes). At 100% of CV, the subjects maintained the running pace for an average of 16.43 +/- 6.08 minutes (range = 9.96-31.90 minutes) while, at 85% of CV, 8 of the 10 subjects were able to maintain the running pace for 60 minutes. These findings did not support the validity of the CV test for predicting the actual TL during treadmill running and indicated that, in 20% of the cases, CV overestimated the running velocity that could be maintained for 60 minutes by greater than 15%.

Adult↗

Ventilatory threshold and maximal oxygen uptake during cycling and running in female triathletes.

Maximal oxygen uptake (VO2max) and the ventilatory threshold (Tvent) were measured during cycle ergometry (CE) and treadmill running (TR) in a group of 10 highly trained female triathletes. Tvent was defined as the VO2 at which the ventilatory equivalent for oxygen increased without a marked rise in the ventilatory equivalent for carbon dioxide. Female triathletes achieved a significantly higher mean (+/- SE) relative VO2max for running (63.6 +/- 1.2 ml.kg-1.min-1) than for cycling (59.9 +/- 1.3 ml.kg-1.min-1). When oxygen uptake measured at the ventilatory threshold was expressed as a percent of VO2max, the mean value obtained for TR (74.0 +/- 2.0% of VO2max) was significantly greater than the value obtained for CE (62.7 +/- 2.1% of VO2max). This occurred even though the total training time and intensity were similar for the two modes of exercise. Female triathletes had average running and cycling VO2max values that compared favorably with maximal oxygen uptake values previously reported for elite female runners and cyclists, respectively. However, mean running and cycling Tvent values (VO2 Tvent as%VO2max) were lower than recently reported values for single-sport athletes. The physiological variability between the triathletes studied and single-sport athletes may be attributed in part to differences in training distance or intensity, and/or to variations in the number of years of intense training in a specific mode of exercise. It was concluded that these triathletes were well-trained in both running and cycling, but not to the same extent as female athletes who only train and compete in running or cycling.

Adult↗

Relationship of training and life-style to 16-km running time of 4000 joggers. The '84 Berne "Grand-Prix" Study.

To investigate running activity, life-style, and endurance capacity of joggers, all competitors of a popular 16-km race were surveyed by questionnaire. The response rate was 83.6%, yielding a study population of 4358 male runners over age 16. In univariate analysis, there were significant associations between 16-km running time and weekly training distance (average of 1 year), weekly training frequency, body mass index (BMI), age, cigarette smoking, years of regular running, and frequency of alcohol cosumption. A multiple regression analysis provided six significant, independent predictors of 16-km time, explaining 47% of its variance: weekly training distance (standardized regression coefficient = -0.46), age (0.37), BMI (0.23), years of regular running (-0.19), weekly training frequency (-0.11), and cigarette smoking (0.10). Based on laboratory treadmill testing of a subsample of runners, 16-km running times were transformed into maximum aerobic capacities (VO2 max equivalents) for all competitors. In comparison with the general population, even the slowest 5% of the runners showed a higher endurance capacity than the age-specific population mean. Application of the multivariate regression model for an estimation of the overall impact of training and life-style on endurance capacity showed that the great difference in mean endurance levels between joggers and the general population could entirely be attributed to differences in running activity, BMI, and smoking. We conclude that the joggers investigated were, on average, not selected concerning biological predisposition and genetic endowment since their behavior alone explained their high average endurance capacity.

Adolescent↗

Submaximal aerobic running economy and treadmill performance in prepubertal boys.

Although the determinants of running economy and its relationship to endurance performance have been evaluated in adults, little data are available in pediatric subjects. Twenty-eight prepubertal boys with diverse athletic abilities underwent progressive maximal treadmill testing. Running economy was defined as (1) VO2 at 9.6 kph and (2) VO2 expenditure for increasing running speed 1.6 kph (delta VO2). Treadmill running times ranged from 12.6 to 23.0 min. Mean VO2 at 6 mph was 48.7 m.kg-1.min-1 (SD 4.3) and delta VO2 averaged 6.8 m.kg-1.min-1 (SD 1.7). VO2max correlated closely to treadmill time. Treadmill endurance times were significantly related to running economy by both definitions; submaximal VO2 levels did not, however, correlate with maximal aerobic power. Delta VO2 was associated with stride frequency, but no other relationships were observed between economy and height, age, respiratory exchange ratio, ventilatory equivalent for oxygen, stride length: leg length ratio, or body surface area: mass ratio. These findings suggest that among older prepubertal boys, greater running economy is associated with superior treadmill endurance performance and that stride frequency may influence submaximal VO2 expenditure in children.

Adolescent↗

Ventilatory responses of trained and untrained subjects during running and walking.

To investigate the influence of stride frequency on ventilation in different subject populations, the ventilatory responses to walking and running at similar metabolic loads were studied in 29 males. Ten of the males were well-trained, highly fit runners (HFR), ten were well-trained, highly fit cyclists (HFC), and nine were healthy low-fit males (LFM) who did not engage in any form of regular exercise. All subjects completed two separate exercise bouts, a level run and an uphill walk, at 90% of their ventilatory threshold. Stride frequency was found to increase by 49% between the walk and run trials. Minute ventilation (VE), tidal volume (VT), breathing frequency (f), end-tibial CO2 tension (PETCO2), end-tidal O2 tension (PETO2), and inspiratory time (TI) were all significantly different (P less than 0.05) between the walk and run trials for the HFR. Minute ventilation and expiratory time (TE) did not differ significantly between the walk and run trials for the HFC; however, VT, f, PETCO2, PETO2, and TI were significantly different. Only PETCO2 and TI were found to be significantly different between the walk and run trials for the LFM. These results suggest that stride frequency affects ventilation to varying degrees dependent upon the subject population and that the mechanisms for the hyperpnea of moderate exercise operating in each of these subject populations involve a complex interaction of many factors.

Adult↗

Endurance running performance in relation to cardiovascular risk indicators in adolescents.

We evaluated endurance running performance and body mass index (BMI) in relation to biochemical cardiovascular disease (CVD) risk indicators (s-lipids, s-insulin, s-ferritin), and blood pressure in 14- and 17-year-old healthy Swedish adolescents (n = 879), also considering current dietary intake and physical activity. Endurance running performance was assessed using a 3 km running test and height, body weight, waist and hip circumference, and skinfolds were measured at clinical examination. Physical activity and dietary intake were evaluated using self-reported 7-day-records. The results showed that high endurance running performance was related to a favourable CVD risk indicator profile. Multiple regression analyses including running time, BMI, physical activity, dietary fat and iron intake, and age as independent and s-lipids, s-insulin, and s-ferritin as dependent variables revealed that, in both boys and girls, low BMI was associated to a favourable s-lipid profile and lower s-insulin values and, in boys but not in girls, also to lower s-ferritin values. There were, however, no independent associations between level of physical activity or endurance running performance on the one side and s-lipids, s-insulin, or s-ferritin values on the other. High dietary fat intake was associated to s-lipids in a non-atherogenic direction; in boys to higher HDL-C and in girls to lower TG. In conclusion the study showed that body mass seems to be the most important factor explaining the differences in s-lipid and s-insulin values between adolescents with different level of physical performance capacity. An interesting finding was, that s-ferritin, being a proposed risk factor for CVD, in the older boys related to body mass in a similar way as s-lipids and s-insulin.

Adolescent↗

Physiologic correlates to running performance in pre-pubertal distance runners.

In adults, four major variables have been shown to be associated with success in distance running performance: submaximal oxygen consumption (running economy), peak oxygen consumption (Peak VO2), ventilatory threshold (VT) and fractional utilisation (FU). The primary aim of this study was to describe the relationship between the 3000 m race times of run-trained prepubertal boys to these four variables. Thirteen male run-trained pre-pubertal boys (age 11.7 +/- 1.1 yrs, mean +/- SD), volunteered to take part in a 3000 m time trial and laboratory assessment, consisting of treadmill running at four submaximal speeds (8, 9.6, 11.2 and 12.8 km.h-1) as well as a peak VO2 test. The group demonstrated a heterogeneous array of peak VO2 data. A high level of association (p < 0.05) was found between mass-relative peak VO2 and 3000 m time trial results (r = -0.83). In addition ventilatory threshold expressed as %peak VO2, VO2 at VT and estimated velocity at VT was also highly related to 3000 m time trial (r = -0.78, -0.77 and -0.77) respectively. Fractional utilisation (%peak VO2) was significantly (p < 0.05) associated with race time at the final two submaximal running speeds only (11.2 and 12.8 km.h-1) (r = 0.61 and 0.67, respectively). Respiratory Exchange Ratio (RER) was also found to be significantly (p < 0.05) associated with 3000 m race time at 11.2 and 12.8 km.h-1. Overall peak VO2 appeared to be the single most important factor associated with success at 3000 m.

Adolescent↗

Effects of treadmill running on glycosaminoglycans in articular cartilage of rabbits.

In a study of the effects of motion load on weight-bearing articular cartilage, rabbits were made to run on a treadmill for 1-, 5-, and 30-day periods. One group ran on a level surface, and another ran uphill. In vivo 35S-sulfate bound in glycosaminoglycans (GAG) after running was used as an indicator of the synthesis rate of GAGs. The GAG content of the tibial and femoral cartilages was measured from determinations of uronic acid, galactosamine, and glucosamine in GAGs after papain proteolysis and purification. Uphill running caused a depletion of the bound 35S-sulfate incorporated in GAGs in both tibial and femoral weight-bearing cartilage (5 days: P less than 0.001 and P less than 0.01, respectively; 30 days, tibial cartilage: P less than 0.01). The concentrations of uronic acid and galactosamine were mainly unaltered, except after 1 day of running when an increased concentration of both substances was found, particularly in rabbits running on a level surface (uronic acid: P less than 0.05; galactosamine: P less than 0.001). After 30 days of running the glucosamine content was markedly reduced in GAGs of both tibial and femoral cartilage (P less than 0.05). The possible role of different factors affecting the cartilage metabolism is discussed.

Animals↗

High level runners are able to maintain a VO2 steady-state below VO2max in an all-out run over their critical velocity.

During prolonged and intense running exercises beyond the critical power level, a VO2 slow component elevates VO2 above predicted VO2-work rates calculated from exercise performed at intensities below the lactate threshold. In such cases, the actual VO2 value will increase over time until it reaches VO2max. The aims of the present study were to examine whether the VO2 slow component is a major determinant of VO2 over time when running at a speed beyond critical velocity, and whether the exhaustion latency period at such intensity correlates with the magnitude of the VO2 slow component. Fourteen highly trained long-distance runners performed four exhaustive runs, each separated by one week of light training. VO2 and the velocity at VO2max (vVO2max) were determined for each by a graded treadmill exercise. The critical velocity (86.1 +/- 1.5% vVO2max) of each runner was calculated from exhaustive treadmill runs at 90, 100 and 105% of vVO2max. During supra-critical velocity runs at 90% of vVO2max, there was no significant rise in VO2max (20.9 +/- 2.1 ml min-1 kg-1 between the third and last min of tlim 90), such that the runners reached a VO2 steady-state, but did not reach their vVO2max level over time (69.5 +/- 5.0 vs 74.9 +/- 3.0 ml min-1 kg-1). Thus, subjects' time to exhaustion at 90% of vVO2max was not correlated with the VO2max slow component (r = 0.11, P = 0.69), but significantly correlated with the lactate threshold (r = 0.54, P = 0.04) and the critical velocity (% vVO2max; r = 0.65, P = 0.01). In conclusion, the present study demonstrates that for highly trained long-distance runners performing exhaustive, supra-critical velocity runs at 90% of vVO2max, there was not a VO2 slow component tardily completing the rise of VO2. Instead, runners will maintain a VO2 steady-state below VO2max, such that the time to exhaustion at 90% of vVO2max for these runners is positively correlated with the critical velocity expressed as % of vVO2max.

Adult↗

Energy cost of running in young and adult female athletes.

Maximal oxygen uptake (VO2 max.kg-1) and energy cost of running were determined on the treadmill in groups of differently trained young and adult athletes. The VO2 max.kg-1 was in all cases higher in adults than in young athletes. These differences were significant (p < 0.05) in long-distance runners (n = 12, mean age = 24.2 +/- 2.2 vs 17.3 +/- 0.9 yrs, mean VO2 max.kg-1 = 66.9 +/- 4.2 vs 58.2 +/- 4.3 ml.min-1.kg-1), and in middle-distance runners (10, 22.9 +/- 2.8 vs 16, 16.6 +/- 0.8, 62.3 +/- 3.7 vs 56.1 +/- 2.8); in canoeists these differences were non-significant (7, 21.1 +/- 2.1 vs 16.0 +/- 2.3 vs 8, 48.2 +/- 2.6). Values of energy cost of running--coefficients of energy demand of running c, which indicates how much energy is required to transfer 1 kg of body mass on a distance of 1 m--were lower in adult athletes than in young athletes. These differences were significant (p < 0.05) only in long-distance runners (3.69 +/- 0.15 vs 3.84 +/- 0.14 J.kg-1.m-1). In middle-distance runners (3.67 +/- 0.19 vs 3.76 +/- 0.18), and in canoeists (3.84 +/- 0.14 vs 3.86 +/- 0.18) these differences were non-significant. It is concluded that the differences in energy cost of running between trained adult and young female athletes are probably associated with differences in adaptation to the running, and with the technique of movement. Differences in running speed (sports performance) between adult and young athletes are associated with differences in VO2 max.kg-1 and c.

Adolescent↗

Fluid intake in male and female runners during a 40-km field run in the heat.

To compare physiological responses, hydration status and exercise performance in similarly trained men and women in a hot, humid environment, 12 highly trained runners were studied during a simulated 40-km race. A 7% carbohydrate-electrolyte (CE) beverage was consumed prior to exercise (400 ml) and every 5 km (approximately 250 ml) during the run. The run times of the males and females did not differ significantly (173.5 +/- 8.5 and 183.8 +/- 4.2 min, respectively); nor did the rate of fluid intake relative to body mass (10.3 +/- 0.7 and 10.7 +/- 0.8 ml kg-1 h-1, respectively) or percent body mass loss (4.0 +/- 0.1% and 3.9 +/- 0.1%, respectively). During the run, %VO2 max, heart rate, concentrations of blood lactate, serum total protein and plasma osmolality were also similar for both groups. However, some significant sex differences (P < 0.05) were observed: the females had lower plasma volume losses and higher serum potassium and sodium concentrations than the males during the run. Rectal temperatures were lower in the female runners compared with the males during the last 10 km of the run (0.7 degrees C) and recovery (1.1 degrees C). Findings from this 40-km field run in hot, humid conditions suggest that CE fluid replacement at a relatively similar dosage (approximately 10 ml kg-1 h-1) may have sex-specific physiological effects. These observations warrant further investigation to assess the need for sex-specific fluid replacement guidelines.

Adult↗