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Trehalases from spores and vegetative cells of yeast Saccharomyces cerevisiae.

Trehalase (THA) activity from S. cerevisiae spores and vegetative cells could be differentiated in cell-free extracts. THA from the vegetative cells has an optimal activity at neutral pH whereas biphase pH optimum in the spores was observed. The enzyme from the spores exhibited higher thermostability than that from the vegetative cells. The presence of magnesium ions was necessary mainly for THA activity from the vegetative cells. The effect of the other metal ions studied: Hg2+, Ag2+, Cu2+, Fe3+, Ni2+, Cd2+ etc. (Table II), on THA from both sources was almost the same, however, the spores THA was resistant to Pb2+ and especially to Zn2+. Moreover, the influence of inorganic polyphosphates and polyamines was also quite dissimilar. Polyphosphates inhibited THA from the vegetative cells and to a smaller extent from the spores. On the other hand, polyamines stimulated highly THA activity from vegetative yeast cells in contrast to spores one. The effect of these ions modulators would facilitate differentiating of THA activity in the cell-free extracts from both sources. These data could be interpreted as phenotypic reflections of trehalase genes expression in the S. cerevisiae cells.

Culture Media↗

Animal-vegetal asymmetries influence the earliest steps in retina fate commitment in Xenopus.

An individual retina descends from a restricted and invariant group of nine animal blastomeres at the 32-cell stage. We tested which molecular signaling pathways are responsible for the competence of animal blastomeres to contribute to the retina. Inactivation of activin/Vg1 or fibroblast growth factor (FGF) signaling by expression of dominant-negative receptors does not prevent an animal blastomere from contributing to the retina. However, increasing bone morphogenetic protein (BMP) signaling in the retina-producing blastomeres significantly reduces their contribution. Conversely, reducing BMP signaling by expression of a dominant-negative BMP receptor or Noggin allows other animal blastomeres to contribute to the retina. Thus, the initial step in the retinal lineage is regulated by position within the BMP/Noggin field of epidermal versus neural induction. Vegetal tier blastomeres, in contrast, cannot contribute to the retina even when given access to the appropriate position and signaling fields by transplantation to the dorsal animal pole. We tested whether expression of molecules within the mesoderm inducing (activin, FGF), mesoderm-modifying (Wnt), or neural-inducing (BMP, Noggin) pathways impart a retinal fate on vegetal cell descendants. None of these, several of which induce secondary head structures, caused vegetal cells to contribute to retina. This was true even if the injected blastomeres were transplanted to the dorsal animal pole. Two pathways that specifically induce head tissues also were investigated. The simultaneous blockade of Wnt and BMP signaling, which results in the formation of a complete secondary axis with head and eyes, did not cause the vegetal clone to give rise to retina. However, Cerberus, a secreted protein that also induces an ectopic head with eyes, redirected vegetal progeny into the retina. These experiments indicate that vegetal blastomere incompetence to express a retinal fate is not due to a lack of components of known signaling pathways, but relies on a specific pathway of head induction.

Activins↗

Hematological and lipid changes in newborn piglets fed milk replacer diets containing vegetable oils with different levels of n-3 fatty acids.

To test if linolenic acid (18:3n-3) from vegetable oils would affect bleeding times and platelet counts in newborns, piglets were used as a model fed milk replacer diets containing 25% (by wt) vegetable oils or oil mixtures for 28 d and compared to sow-reared piglets. The oils tested included soybean, canola, olive, high oleic sunflower (HOAS), a canola/coconut mixture and a mixture of oils mimicking canola in fatty acid composition. All piglets fed the milk replacer diets showed normal growth. Bleeding times increased after birth from 4-6 min to 7-10 min by week 4 (P < 0.001), and were higher in pigs fed diets containing 18:3n-3, as well as in sow-reared piglets receiving n-3 polyunsaturated fatty acids (PUFA) in the milk, as compared to diets low in 18:3n-3. Platelet numbers increased within the first week in newborn piglets from 300 to 550 x 10(9)/L, and remained high thereafter. Milk replacer diets, containing vegetable oils, generally showed a transient delay in the rise of platelet numbers, which was partially associated with an increased platelet volume. The oils showed differences in the length of delay, but by the third week of age, all platelet counts were > 500 x 10(9)/L. The delay in rise in platelet counts appeared to be related to the fatty acid composition of the oil, as the effect was reproduced by a mixture of oils with a certain fatty acid profile, and disappeared upon the addition of saturated fatty acids to the vegetable oil. There were no alterations in the coagulation factors due to the dietary oils. Blood plasma, platelets and red blood cell membranes showed increased levels of 18:3n-3 and long-chain n-3 PUFA in response to dietary 18:3n-3. The level of saturated fatty acids in blood lipids was generally lower in canola and HOAS oil-fed piglets as compared to piglets fed soybean oil or reared with the sow. The results suggest that consumption of milk replacer diets containing vegetable oils rich in 18:3n-3 does not represent a bleeding risk, and that the transient lower platelet count can be counterbalanced by the addition of saturated fatty acids to the vegetable oils.

Animals↗

Analysis of spatial and temporal evolution of vegetation cover in the Spanish Central Pyrenees: role of human management.

A vegetation cover increase has been identified at global scales using satellite images and vegetation indices. This fact is usually explained by global climatic change processes such as CO(2) and temperature increases. Nevertheless, although these causes can be important, the role of socioeconomic transformations must be considered in some places, since in several areas of Northern Hemisphere an important change in management practices has been detected. Rural depopulation and land abandonment have reactivated the natural vegetation regeneration processes. This work analyses the vegetation evolution in the central Spanish Pyrenees from 1982 to 2000. The analysis has been done by using calibrated-NDVI temporal series from NOAA-AVHRR images. A positive and significant trend in NDVI data has been identified from 1982 to 2000 coinciding with a temperature increase in the study area. However, the spatial differences in magnitude and the sign of NDVI trends are significant. The role of land management changes in the 20th century is considered as a hypothesis to explain the spatial differences in NDVI trends. The role of land-cover and human land-uses on this process has been analyzed. The highest increment of NDVI is detected in lands affected by abandonment and human extensification. The importance of management changes in vegetation growth is discussed, and we indicate that although climate has great importance in vegetal evolution, land-management changes can not be neglected in our study area.

Altitude↗

Relationship between tourism development and vegetated landscapes in Luya Mountain Nature Reserve, Shanxi, China.

The relationship between tourism development and vegetated landscapes is analyzed for the Luya Mountain Nature Reserve (LMNR), Shanxi, China, in this study. Indices such as Sensitive Level (SL), Landscape Importance Value (LIV), information index of biodiversity (H'), Shade-tolerant Species Proportion (SSP), and Tourism Influencing Index (TII) are used to characterize vegetated landscapes, the impact of tourism, and their relationship. Their relationship is studied by Two-Way Indicator Species Analysis (TWINSPAN) and Detrended Correspondence Analysis (DCA). TWINSPAN gives correct and rapid partition to the classification, and DCA ordination shows the changing tendency of all vegetation types based on tourism development. These results reflect the ecological relationship between tourism development and vegetated landscapes. In Luya Mountain Nature Reserve, most plant communities are in good or medium condition, which shows that these vegetated landscapes can support more tourism. However, the occurrence of the bad condition shows that there is a severe contradiction between tourism development and vegetated landscapes.

China↗

Age at flowering differentially affects vegetative and reproductive responses of a determinate annual plant to elevated carbon dioxide.

Plant population and community dynamics may be altered by increasing atmospheric CO(2) concentrations [[CO(2)]] through intraspecific variation in the responses of vegetative and reproductive growth. Although these responses may be regulated by age at flowering, little is known about the direct effects of age at flowering on growth responses to elevated [CO(2)]. In this study, we examined the interactive effects of elevated [CO(2)] and age at flowering on absolute and relative allocation to vegetative and reproductive growth in the determinate, short-day species Xanthium strumarium L. (common cocklebur). Six cohorts were planted at 5-day intervals in chambers maintained at either 365 or 730 micro mol mol(-1) CO(2), with an 18-h photoperiod and a non-limiting nutrient supply. All plants were simultaneously induced to flower by switching the photoperiod to 12 h for 2 days, then switching back to an 18-h photoperiod for the remainder of the experiment. All plants were harvested 15 days after the onset of flowering. Total plant biomass increased 11-41% with increasing [CO(2)] and 45% from the youngest to the oldest cohort. Vegetative growth responses to elevated [CO(2)] significantly increased with increasing age at flowering, associated with increasing sink relative to source capacity. In contrast, total fruit mass decreased 32% from the youngest to the oldest cohort and was not significantly affected by CO(2) supply. Relative biomass allocation to fruit decreased 47% from the youngest to the oldest cohort, reflecting decreased numbers of fruit, and 6-28% with increasing [CO(2)], reflecting decreased mean mass per mature fruit. Our findings suggest that elevated [CO(2)] may increase vegetative growth in Xanthium without increasing reproductive biomass, and that age at flowering may influence these responses through effects on source:sink balance. Further, changes in the allometric relationship between vegetative and reproductive growth associated with growth in elevated [CO(2)] suggest that long-term population and community-level responses to elevated [CO(2)] may differ substantially from predictions based on vegetative responses.

Carbon Dioxide↗

Nutritional differences and leaf acclimation of climbing plants and the associated vegetation in different types of an Andean montane rainforest.

Climbing plants are known to play an important role in tropical forest systems, but key features for their distribution are only partly understood. Investigation was carried out to find if climbers differ from self-supporting vegetation in their adjustment of leaf parameters over a wide variety of light regimes in different forest types along an altitudinal gradient. Relative photon flux density (PFDrel) was assessed above 75 pairs of strictly linked climbers and supporting vegetation on seven plots between 2,020 and 2,700 m a.s.l. along a mountain range in South-Ecuador up to the Páramo vegetation. Leaf samples from both growth forms were analyzed for leaf area (LA), specific leaf mass (LMA), mass and area-based carbon and nitrogen concentration (C, Carea, N, and Narea) and concentrations of P, K, Ca, Mg, Mn and Al. Leaf size of climbers was independent of general light condition, whereas the leaf size of the self-supporting vegetation increased in shade. LMA increased as expected with altitude and irradiance for both growth forms, but climbers generally built smaller leaves with lower LMA. N, P, and K concentrations were higher in the leaves of climbers than in their supporters. Relationships of LMA and Narea to the light conditions were more pronounced within the climbers than within their supporters. Slope for the regression between climber's Narea and LMA was twice as steep as for the supporter leaves. Al accumulators were only found within the self-supporting vegetation. The investigated traits indicate improved adjustment towards light supply within climbers compared to self-supporting vegetation. Thus climbing plants seem to have a higher potential trade off in resource-use efficiency regarding irradiance and nutrients.

Peru↗

Selection on floral display in insect-pollinated Primula farinosa: effects of vegetation height and litter accumulation.

Grazing reduces litter thickness and vegetation height and may thereby indirectly affect reproductive success and selection on floral characters in plants with prostrate growth. Reductions in litter thickness and vegetation height should influence both the resource status of plants with leaves positioned close to the ground and the significance of inflorescence height for interactions with pollinators and seed predators. We experimentally examined how simulated grazing of surrounding vegetation affected pollen limitation, fruit predation and fecundity of short-scaped and long-scaped Primula farinosa, which differ markedly in floral display and therefore in expected attractiveness to pollinators. Litter removal and pruning of surrounding vegetation increased fruit and seed production per plant in the year of the treatment and the probability of flowering in the following year. Pollen limitation of fruit and seed production was stronger in the short-scaped morph than in the long-scaped morph, but was not significantly affected by litter removal and simulated grazing of surrounding vegetation. Supplemental hand-pollination reduced seed size in the year of the treatment and flowering probability in the second year, and these effects did not differ among scape morphs or grazing treatments. The results suggest that grazing indirectly favours seed production in P. farinosa, mainly because it increases the resource status of plants that escape damage. Contrary to expectation, there was no strong evidence that litter accumulation and tall vegetation increase the severity of pollen limitation or reduce the relative performance of the short-scaped morph.

Animals↗

The natural vegetation responses to the groundwater change resulting from ecological water conveyances to the lower Tarim River.

This paper takes the ecological water conveyance project (EWCP) that transfers water from the Bosten Lake, to Daxihaizi Reservoir, and finally to the Taitema Lake as a case study to analyze the dynamic change of the groundwater depth, the vegetation responses to the elevation of the groundwater depth as well as the relationship between the groundwater depth and the natural vegetation. The results from many years' monitoring in field indicate: (1) the groundwater depth has been elevating gradually with the increase in the times of watering and the elevation range has been expanding continuously in the lower reaches of Tarim River. Correspondingly, the natural vegetation has a favorable response to the elevation of the groundwater depth. The change of the natural vegetation has accordance with that of the groundwater depth. Such facts not only show that groundwater is a key factor to the growth of the native vegetation but also prove it is feasible that the degraded ecosystem can be restored and protected by the EWCP; (2) the results of analysis of the spatial-temporal response of the natural vegetation to watering reveals that the beneficial influence of the EWCP on the ecosystem in the lower Tarim River is a long-term process; (3) in terms of the function and structure of ecosystem after watering in the lower reaches of Tarim River, the EWCP does not still reach the goal of ecological restoration at a large spatial scale at present. Based on such monitoring results, some countermeasures and suggestions for the future restoration strategy are proposed so as to provide a theoretical basis for restoring and protecting the ecosystem in Tarim River, and meanwhile it can also provide some scientific references for implementing the similar ecological projects in other areas.

Agriculture↗

Relation between the presence of echocardiographic vegetations and the complication rate in infective endocarditis.

M-mode and two-dimensional echocardiograms of 77 patients with infective endocarditis were examined to determine if presence and/or size of vegetations on echocardiogram were predictive of morbidity and mortality. Patients with (n = 43) or without (n = 34) vegetations on echocardiogram did not differ significantly in the proportions developing congestive heart failure (23 of 43 or 53% vs 12 of 34 or 35%) or emboli (11 of 43 or 24% vs 6 of 34 or 18%), whereas a slightly lower proportion of those with vegetations required surgery (5 of 43 or 12% vs 7 of 34 or 21%) or died (3 of 43 or 7% vs 4 of 34 or 12%). No significant relationship was found between vegetation size and the frequency of complications, the need for surgery, or death. In contrast, patients whose echocardiograms demonstrated premature mitral valve closure or chordal or cusp rupture had a significantly higher incidence of heart failure (10 of 13 or 77% vs 22 of 60 or 37%, p less than 0.003) and surgery (3 of 13 or 23% vs 7 of 60 or 12%, p less than 0.05). We conclude that: the presence of vegetation on the initial echocardiogram is not predictive of the clinical course in infective endocarditis; vegetation size does not predict complications, need for surgery, or death; but valve cusp or chordal rupture and/or premature mitral valve closure are associated with congestive heart failure and the need for surgery.

Adolescent↗

Genotoxic effects of crude juices from Brassica vegetables and juices and extracts from phytopharmaceutical preparations and spices of cruciferous plants origin in bacterial and mammalian cells.

Crude juices of eight Brassica vegetables as well as juices and extracts of spices and phytopharmaceutical preparations from cruciferous vegetables were tested for induction of point mutations in Salmonella TA98 and TA100, repairable DNA damage in E.coli K-12 cells and clastogenic effects in mammalian cells. In bacterial assays, all juices caused genotoxic effects in the absence of metabolic activation, the ranking order being: Brussels sprouts > white cabbage > cauliflower > green cabbage > kohlrabi > broccoli > turnip > black radish. In experiments with mammalian cells, six juices induced structural chromosome aberrations. Brussels sprouts, white and green cabbage caused the strongest effects (800 microliters of juice induced a 5-fold increase over the background). In sister chromatid exchange assays, positive results were measured as well, but the effects were less pronounced. With all juices the genotoxic effects seen in mammalian cells were paralleled by a pronounced decrease in cell viability. Column fractionation experiments showed that 70-80% of the total genotoxic activity of the juices is found in the fraction which contains isothiocyanates and other breakdown products of glucosinolates, whereas phenolics and flavonoids contributed to a lesser extent to the overall effects. On the basis of these findings, and considering the negative results obtained with non-cruciferous vegetables (tomato, carrot and green pepper), it seems likely that the genotoxic effects of the juices are due to specific constituents of cruciferous plants such as glucosinolates and/or their breakdown products, in particular, isothiocyanates, which we found previously to be potent genotoxins in bacterial and mammalian cells. Finally, spices (mustards and horse radish paste) and phytopharmaceutical preparations were tested in bacterial assays. Mustards and horse radish caused very weak effects while most of the pharmaceutical preparations gave negative results, except cabbage tablets, which caused a strong and dose dependent induction of his revertants in Salmonella TA100. The present findings clearly indicate that cruciferous vegetables contain DNA damaging constituents. These observations are in contrast to earlier findings, which emphasized the antimutagenic effects of vegetable juices and also raise the question whether greatly increased consumption of Brassica vegetables or their concentrated constituents as a means for cancer prevention is indeed recommendable.

Animals↗

Animal-vegetal polarity in the plasma membrane of a molluscan egg: a quantitative freeze-fracture study.

Using freeze-fracture electron microscopy, the numerical particle distribution in the fertilized Nassarius egg plasma membrane has been analyzed in four areas at different positions along the animal-vegetal axis of the egg. These areas can be distinguished by distinct microvilli patterns and differences in microvilli densities. In all areas, more IMPs (intramembrane particles) are present on the P face than on the corresponding E face. The ratio of the number of IMPs present on E and P face is similar in all areas (0.48-0.55) except for the most animal part of the vegetal hemisphere, where relatively more IMPs remain attached to the exterior half of the fractured membrane (E/P ratio = 0.88). The IMP density at the vegetal pole of the egg is considerably higher than in the animal hemisphere and in the animal part of the vegetal hemisphere. This difference is due to an increased number of IMPs in all size classes (4-18 nm). In the area adjacent to the vegetal pole the density of particles is also higher than in the two more animal areas, but here the difference is exclusively due to the smaller IMP size classes (4-8 nm). Statistical analysis of our data reveals that the area adjacent to the vegetal pole patch is significantly different from the other areas with respect to the distribution of the IMPs over the different IMP size classes. These results demonstrate the polar organization of the Nassarius egg plasma membrane. The possible role of this surface heterogeneity in the spatial organization of the egg cell and the later embryo is discussed.

Animals↗

Computation of drug concentrations in endocardial vegetations in patients during antibiotic therapy.

The treatment of endocarditis often requires prolonged antibiotic therapy. Individualized drug dosage regimens have made such therapy possible even in patients with impaired renal function. However, the problem of efficacy remains. Especially for aminoglycosides, it would be a useful guide to have at least an approximate idea of the concentration of an antibiotic within an endocardial vegetation. This study was designed to develop software to model the drug concentrations at different layers within spherical vegetations to provide a guide during clinical therapy of patients with endocarditis. A general model describing the diffusion of antibiotics in spheres has now been developed and interfaced with the USC*PACK PC Clinical Programs in order to compute and plot concentrations, within the vegetation, based on the regimen given to the patient and the diffusitivity of the antibiotic into the vegetation. Some preliminary results of this research, which are still in progress, are presented. Diffusion into simulated spherical vegetations has been computed for different treatment regimens for endocarditis: amikacin or netilmicin and vancomycin were given to three elderly patients (3 women, 74, 75 and 92 years old, with initial estimated creatinine clearances of 51, 36, and 31 ml/min/1.73 m2, respectively). Although Amikacin has a low diffusivity, the concentrations, even in the center of the vegetation, appear to be effective. The effects of various regimens, including a 'once-a-day' aminoglycoside regimen, are presented.

Aged↗

Hydrocarbon and elemental carbon signatures in a tropical wetland: biogeochemical evidence of forest fire and vegetation changes.

Evidence of changing vegetation in the tropical wetland (Rawa Danau, west Java, Indonesia) over the past 7428 years is illustrated by elemental (soot) carbon (EC) and n-alkane composition of sedimentary geolipids. In this study, vegetation changes and relevant controlling factors (e.g. forest fire and climate change) were documented on a decadal to centennial scale. The n-alkane composition that changes with depth might record changes in sources of organic matter (OM) in the wetland. The presence of EC (0.01-0.24% of organic carbon: OC) during late (0-1700 cal. year BP) and mid (3500-4500 cal. year BP) Holocene (at depths 0-50 cm, and 160-210 cm) indicated that large-scale forest fires severely affected the tropical vegetation. The hydrocarbon indices (CPI: carbon preference index, MCN: mean carbon number, and HVI: hydrocarbon vegetation index) significantly correlated with one another while a comparison of EC profile with the profiles of hydrocarbon indices indicated that n-alkane composition of the geolipid in lake sediment could record signatures of changes in catchment vegetation. Forest fire and vegetation changes might be related to regional climatic shifts relating to ENSO activity as well as being influenced by human influences.

Alkanes↗

Recovery of Bacillus thuringiensis in vegetative form from the phylloplane of clover (Trifolium hybridum) during a growing season.

Two media were developed which specifically allow the cultivation of Bacillus thuringiensis while it is in the vegetative as opposed to the spore form. Using these media B. thuringiensis was shown conclusively for the first time to exist in an active form on the phylloplane. The profile of its appearance in vegetative and spore form was followed over a growing season on clover (Trifolium hybridum) in the field. Three simultaneous and sudden rises and declines of both spore and vegetative cell densities were observed. The most common other spore-former on these leaves was Bacillus cereus but the fluctuations in appearance of these two very closely related species were not co-incident. Using specific PCR primers a considerable diversity of cry toxin gene types was found in isolates that had been recovered in vegetative form ('vegetative isolates') with the majority possessing multiple delta-endotoxin genes while some had only one of those tested. Bioassays against a lepidopteran insect of purified delta-endotoxins showed that they were no more potent than those from a laboratory-adapted strain. PCR primers for an internal region of the vip3A gene produced amplification in 70% of the vegetative isolates compared to 25% of the laboratory-adapted strains tested.

Animals↗

Biomarker signature in tropical wetland: lignin phenol vegetation index (LPVI) and its implications for reconstructing the paleoenvironment.

Organic matter of a peat core (3.60 m, 7428 years BP) collected from Rawa Danau, west Java, Indonesia, was analyzed to evaluate the early diagenetic fates of lignin in a tropical wetland and to reconstruct past vegetation and climate changes. Vertical profiles of (Ad/Al)v, (Ad/Al)s, and lambda(8) show that the lignin composition is well preserved in a sub-aqueous environment under reducing conditions. The sedimentary terrigenous plant material at Rawa Danau is comprised predominantly of angiosperm wood. For this kind of tropical, diverse, and dynamic ecosystem, a new vegetation change index called lignin phenol vegetation index (LPVI): LPVI is defined using the lignin phenol composition. This index can sensitively detect terrestrial vegetation changes as well as environmental conditions forcing such changes. The LPVI of the Rawa Danau peat core provides better resolution than other lignin parameters used previously, and reveals four major vegetation change events since the mid-late Holocene. In comparison to other geochemical data (i.e. elemental carbon, isotopes, and hydrocarbons), the LPVI is more sensitive and is able to trace even minor vegetation and climate changes and thus could improve biogeochemical interpretations of peat records.

Biomarkers↗

Cardiac valvular vegetations in cancer patients: a prospective echocardiographic study of 200 patients.

PURPOSE: Nonbacterial thrombotic endocarditis can complicate various malignancies and may cause morbidity and mortality mainly as a result of systemic embolism. The antemortem diagnosis of nonbacterial thrombotic endocarditis is rare. The purpose of our study was to assess the frequency, echocardiographic characteristics, and clinical correlation of nonbacterial thrombotic endocarditis in cancer patients. PATIENTS AND METHODS: A prospective echocardiographic screening of 200 nonselected ambulatory patients with solid tumors was performed. Patients were evaluated for evidence of thromboembolic events and for plasma D-dimer levels. A cohort of 100 consecutive patients without overt heart disease referred to echocardiography for the detection of an occult arterial embolic source served as a control group. It consisted of 52 males and 48 females, median age 60 years. RESULTS: The study group included 87 women and 113 men, median age 64 years (range 21 to 91). The frequent malignancies were lymphoma (26%), carcinoma of the gastrointestinal tract (20%), and carcinoma of the lung (16%). Cardiac valvular vegetations were found in 38 patients (19%) compared with only in 2 patients in the control group (2%, P < 0.001). Vegetations were found on the mitral or on the aortic valve in 19 and 18 patients, respectively. Isolated tricuspid valve vegetation was found in 1 patient. Valvular lesions were mostly common in patients with carcinoma of the pancreas (3 of 6, 50%), carcinoma of the lung (9 of 32, 28%), and lymphoma (10 of 52, 19%). Thromboembolism was diagnosed in 22 (11%) patients (12 deep vein thrombosis, 4 emboli to extremities, 2 cerebrovascular accidents, and 4 "silent" segmental left ventricular wall motion abnormalities on echocardiography). Thromboembolism was noticed in 9 of 38 patients (24%) with vegetations compared with 13 of 162 patients without vegetations (8%; P = 0.013). Plasma D-dimer level was examined in a subgroup of 170 patients. D-dimer level was increased in 19 of 21 patients (90%) with thromboembolism compared with 76 of 149 patients without thromboembolism (51%; P = 0.001). CONCLUSIONS: This study demonstrated a high prevalence of cardiac valvular lesions in patients with solid tumors. Vegetations were associated with thromboembolism. Plasma D-dimer level was significantly increased in patients with thromboembolism.

Adult↗

Lateglacial and Early Holocene vegetation history of the northern Wetterau and the Amöneburger Basin (Hessen), central-west Germany.

The Lateglacial and Early Holocene vegetation history of the northern Wetterau and Amöneburger Basin, two intra-montane basins in Hessen, central-west Germany, is reconstructed by means of pollen and macrofossil analyses. Regional pollen assemblage zones are defined for the Lateglacial and Early Holocene. After calibration of the radiocarbon dates and establishment of age/depth relationships, the ages of the pollen zone boundaries are calculated. The regional vegetation changes correlate closely with the major fluctuations in the delta18O curve of the Greenland ice cores spanning the same time period. During the early Lateglacial, the open herbaceous vegetation with dwarf shrubs in the northern Wetterau was replaced by woodlands. Initially these woodlands consisted of birch, but after the immigration of pine, mixed forests of pine and birch developed. Soon after its immigration pine became dominant and pine woodlands largely replaced the birch forests. Only on the locally wetter substrates of the river floodplain did Betula stands persist. Gradually the importance of herbaceous communities declined and the pine woodlands lost their open character. During the Lateglacial two regressive phases in the vegetation succession are reflected in the data which are equated with the Older- and Younger Dryas biozones. At the beginning of the Younger Dryas, the forest-limit was lowered and the importance of herbaceous communities increased. Later, pine woodlands thinned and Ericales became part of the vegetation, indicating the development of more acid, nutrient-poor soils. A subdivision of the Younger Dryas biozone into a wetter, colder first part and a drier, warmer second part is suggested. At the beginning of the Early Holocene, pine woodlands became more closed and soils more stabilised. The transition between the Younger Dryas and Preboreal biozones is indicated by a lithological change to organic (-rich) deposits. Betula stands persisted on the locally wetter substrates of the fluvial plains. At the end of the early Preboreal, there was an extension of Betula stands on the river floodplains. This is interpreted as a phase in which climate was temporarily wetter, perhaps a reflection of a short-term climatic oscillation, the so-called Preboreal oscillation. During the Preboreal biozone, ferns became more important in the local vegetation of the mires, and later during the Boreal they formed the undergrowth of swamp forests. During the Boreal biozone, a major expansion of hazel took place in both areas. On the hills and slopes of the northern Wetterau and the Amöneburger Basin, open pine forests and hazel scrub developed with grasses and Pteridium in the field layer. In the river valleys, pine forests were replaced by deciduous mixed forests with oak, elm and later also with lime.

Journal Article↗