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At least 685 records · Page 38Linked to original sources
PubMed2004

[The effect of a simulated inflammation procedure in simulated body fluid on bone-like apatite formation on porous HA/beta-TCP bioceramics].

The formation of bone-like apatite on porous HA/beta-TCP bioceramics in dynamic simulated body fluid (SBF) undergoing a simulated inflammation procedure (pH = 6.5) was investigated in order to study the mechanism of osteoinduction and build a new method to choose biomaterials with better bioactivity. The results showed that the surface of porous HA/beta-TCP bioceramics which underwent a simulated inflammation procedure in dynamic SBF was more smooth. The light acidity in the simulated inflammation procedure would dissolve the fine grains and the parts possessing smaller curvature radius on the surface of porous HA/beta-TCP bioceramics, which would reduce the bioceramics solubility. Followed in normal SBF (pH = 7.4), the amount of bone-like apatite formed on the porous HA/beta-TCP bioceramics was less than that of porous HA/beta-TCP bioceramics incubation in normal SBF all along. The results also showed that the amount of bone-like apatite formed on the porous HA/beta-TCP bioceramics sintered by a microwave plasma was more than that of porous HA/beta-TCP bioceramics sintered by a conventional furnace.

Apatites↗
PubMed1994

Detection of HIV-p24 antigen in body fluids by immunotrapping on Staphylococcus aureus (Cowan 1) bacteria, gold immunolabelling and backscattered electron analysis in a scanning electron microscope.

An immunosorbent electron microscopical (ISEM) method, the Protein A-coated bacteria technique/gold, (PA-CBT/G), was developed for the detection of non-particulate soluble antigens. The method is based on immunotrapping of antigens on antibody coated, glutaraldehyde cross-linked, Protein A-rich, Staphylococcus aureus bacteria. The 'trapped' antigen is then identified by colloidal-gold immunolabelling. Gold particles are observed in a scanning/transmission electron microscope by analysis of backscattered electrons. With this method it was possible to detect the presence of p24 HIV antigens in blood, semen, saliva, crevicular and cerebrospinal fluids from HIV seropositive cases. Although the PA-CBT/G identified correctly the p24 antigen in only 80% of the ELISA HIV-antigen positive sera, it detected, more frequently than ELISA, HIV-antigen in seminal and oral fluids. The PA-CBT/G method could thus be useful to fully characterise individual HIV excretion patterns in body fluids other than sera even from patients negative for HIV-antigen by ELISA.

Body Fluids↗
PubMed1998

Body fluid volume status and the renal response to atrial natriuretic peptide in rats.

The effect of altering the volumes of different body fluid compartments on the renal response to atrial natriuretic peptide (ANP) was studied in anesthetized rats before and during administration of the peptide at 170 ng/min. Four different groups were used. In the first (De), reduction of total body water content was induced by 48 h water deprivation. In the second (De+NaCl), an acute intravenous infusion after the same 48 h dehydration was used to restore the extracellular, but not the intracellular, fluid compartment. In the third (Eu+NaCl), euvolemic rats were infused with isotonic saline at the same rate as in group De+NaCl to expand both intravascular and interstitial components of extracellular fluid. In the fourth group (Eu+BSA) an infusion of hyperoncotic (6%) bovine serum albumin in isotonic saline was used to expand the intravascular volume while contracting the interstitial volume. Excretion of water and salt was predictably reduced in the De group compared with the others. This reduction was associated with increased tubular reabsorption, both upstream from the medullary collecting duct and in the duct itself. Administration of ANP did not significantly affect diuresis and saluresis, or tubular transport. By contrast, there were marked and similar diuretic and natriuretic responses to ANP in groups De+NaCl and Eu+NaCl, associated with transport inhibition primarily in the medullary collecting duct. Surprisingly, the rats infused with hyperoncotic solution (Eu+NaCl) also failed to show marked excretory or duct transport responses to ANP. According to the study design, the two nonresponding groups had, respectively, a decreased or a normal intracellular compartment, and a decreased or increased plasma volume. The common feature of both nonresponding groups was a decreased interstitial fluid compartment, whereas the two responding groups had normal or increased interstitial volume. We suggest, therefore, that a replete interstitial fluid compartment is essential for the renal response to ANP.

Animals↗
PubMed1995

Analysis of the components of Lycopus europaeus L. in body fluids during metabolism studies. Comparison of capillary electrophoresis and high-performance liquid chromatography.

During pharmacokinetic studies with extracts obtained from medicinally used plants, analysis in body fluids is mainly performed by HPLC, an established separation method. In this paper high-performance capillary electrophoresis (HPCE) is investigated for its ability to separate such complex extracts. Crude extracts of Lycopus europaeus L. (Lamiaceae) are traditionally used against mild forms of hyperthyroidism. The metabolism of a 70% ethanolic extract with respect to some of its individual main components (rosmarinic and caffeic acid, luteolin-7-glucoside) and a mixture of the pure compounds were investigated using isolated perfused rat liver. After solid-phase extraction metabolites were determined using HPCE and HPLC separation techniques. A buffer solution composed of 0.05 mol l-1 Na2HPO4 at pH 7.0 with 30% acetonitrile was found to be the most suitable electrolyte for HPCE separation. The best mobile phase for isocratic HPLC was 0.03% TFA-acetonitrile (82:18, v/v). Data obtained with HPCE are in good accordance with those from HPLC; HPCE, however, is clearly more rapid and simple to perform.

Animals↗
PubMed2004

Activity of plasma sprayed yttria stabilized zirconia reinforced hydroxyapatite/Ti-6Al-4V composite coatings in simulated body fluid.

Hydroxyapatite (HA)/yttria stabilized zirconia/Ti-6Al-4V bio-composite coatings deposited onto Ti-6Al-4V substrate through a plasma spray technique were immersed in simulated body fluid (SBF) to investigate their behavior in vitro. Surface morphologies and structural changes in the coatings were analyzed by scanning electron microscopy, thin-film X-ray diffractometer, and X-ray photoelectron spectroscopy. The tensile bond strength of the coatings after immersion was also conducted through the ASTM C-633 standard for thermal sprayed coatings. Results showed that carbonate-containing hydroxyapatite (CHA) layer formed on the surface of composite coatings after 4 weeks immersion in SBF solution, indicating the composite coating possessed excellent bioactivity. The mechanical properties were found to decrease with immersion duration of maximum 56 days. However, minimal variation in mechanical properties was found subsequent to achieving supersaturation of the calcium ions, which was attained with the precipitation of the calcium phosphate layers. The mechanical properties of the composite coating were found to be significantly higher than those of pure HA coatings even after immersion in the SBF solution, indicating the enhanced mechanical properties of the composite coatings.

Alloys↗
PubMedDate not supplied

Hydroxyapatite coating on a titanium metal substrate by a discharging method in modified artificial body fluid.

PURPOSE: The aim of this study was to evaluate the formation of a hydroxyapatite coating on titanium plate by discharging in typical and modified artificial body fluids. MATERIALS AND METHODS: Japanese Industrial Standard Grade 2 titanium plates were used as specimens. Discharging was performed on the specimens in each solution, and the surface topography of each coating was observed with a scanning electron microscope. The Ca/P atomic ratio and surface characterization of each coating were evaluated by x-ray photoelectron spectroscopy, and crystal phases of each coating were analyzed by Fourier transform infrared spectroscopy and x-ray diffraction. RESULTS: Tricalcium phosphate formed on the titanium surface in a 1.5 Ca/P solution with high ion concentrations of calcium (2.5 mmol/L) and phosphorous (1.67 mmol/L). Crystalline HA was formed on the titanium surface in a 1.5 Ca/P solution (Hanks' balanced salt solution without organic molecules) with low ion concentrations of calcium (1.26 mmol/L) and phosphorus (0.83 mmol/L). DISCUSSION: A solution containing organic pH buffer was insufficient to form stable coatings on the titanium surface. Coating properties strongly depended on the Ca/P chemical ratio of the applied solution. A Ca/P ratio of 1.5 was appropriate for preparing a calcium phosphate coating on a titanium surface, whereas a Ca/P ratio of 2.5 was excessively high. CONCLUSION: These findings suggest that Hanks' balanced salt solution without organic molecules is the most suitable solution for forming crystalline hydroxyapatite coatings on titanium surfaces by the discharging method.

Body Fluids↗
PubMed2000

Enantioselective determination of drugs in body fluids by capillary electrophoresis.

During the past decade, chiral capillary electrophoresis (CE) emerged as a promising, effective and economic approach for the enantioselective determination of drugs in body fluids, hair and microsomal preparations. This review discusses the principles and important aspects of CE-based chiral bioassays, provides a survey of the assays developed and presents an overview of the key achievements encountered. Applications discussed encompass the pharmacokinetics of drug enantiomers, the elucidation of the stereoselectivity of drug metabolism and bioanalysis of drug enantiomers of toxicological and forensic interest.

Body Fluids↗
PubMed2006

Pharmacokinetics of clarithromycin and concentrations in body fluids and bronchoalveolar cells of foals.

OBJECTIVE: To determine pharmacokinetics of clarithromycin and concentrations in body fluids and bronchoalveolar (BAL) cells of foals. ANIMALS: 6 healthy 2-to 3-week-old foals. PROCEDURES: In a crossover design, clarithromycin (7.5 mg/kg) was administered to each foal via IV and intragastric (IG) routes. After the initial IG administration, 5 additional doses were administered IG at 12-hour intervals. Concentrations of clarithromycin and its 14-hydroxy metabolite were measured in serum by use of high-performance liquid chromatography. A microbiologic assay was used to measure clarithromycin activity in serum, urine, peritoneal fluid, synovial fluid, CSF, pulmonary epithelial lining fluid (PELF), and BAL cells. RESULTS: After IV administration, elimination half-life (5.4 hours) and mean +/- SD body clearance (1.27 +/- 0.25 L/h/kg) and apparent volume of distribution at steady state (10.4 +/- 2.1 L/kg) were determined for clarithromycin. The metabolite was detected in all 6 foals by 1 hour after clarithromycin administration. Oral bioavailability of clarithromycin was 57.3 +/- 12.0%. Maximum serum concentration of clarithromycin after multiple IG administrations was 0.88 +/- 0.19 microg/mL. After IG administration of multiple doses, clarithromycin concentrations in peritoneal fluid, CSF, and synovial fluid were similar to or lower than concentrations in serum, whereas concentrations in urine, PELF, and BAL cells were significantly higher than concentrations in serum. CONCLUSIONS AND CLINICAL RELEVANCE: Oral administration of clarithromycin at 7.5 mg/kg every 12 hours maintains concentrations in serum, PELF, and BAL cells that are higher than the minimum inhibitory concentration (0.12 microg/mL) for Rhodococcus equiisolates for the entire 12-hour dosing interval.

Administration, Oral↗
PubMedDate not supplied

Body fluid shifts in soldiers after a jogging/walking exercise in the heat: effects of water and electrolyte solution on rehydration.

OBJECTIVES: To examine the relationships between dehydration and body fluid shifts and the effects of ingesting water or oral rehydration solution or no fluid during and after exercise in the heat [mean (SE)] temperature, 40.5 (0.66) degrees C and 32 (3.7)% humidity. DESIGN: PRE and POST three hours exercise comparative study. SETTING: Zimbabwe National Army Wafa-Wafa Training Camp, Kariba, Zimbabwe. SUBJECTS: 18 male soldiers volunteered to be studied during and after a 20 km (three hour) jogging/walking exercise in full kit. MAIN OUTCOME MEASURES: Body mass, total body water, extracellular water, intracellular water, plasma osmolality, plasma sodium, and volume changes compared using paired t-test. RESULTS: Total body water decreased by 4.9 (0.38) 1 (p < 0.02) in soldiers exercising without fluid, 1.5 (0.3) 1 (oral rehydration solution), 2.4 (0.8) 1 (water). Extracellular water decreased by 3.6 (0.3) 1 (p < 0.05), 1.3 (0.2) 1, 1.7 (0.3) 1, and intracellular water decreased by 1.3 (0.1) 1, 0.2 (0.01) 1, 0.7 (0.01) 1 respectively in these groups. Plasma volume decreased by [mean (SE)] 16 (1.4)% on no fluid, three (0.3)% on oral rehydration solution, five (0.3)% on water. Plasma osmolality increased significantly from 285 (1.0) to 301 (2.3) mosmol.kg-1 (p < 0.001) in subjects exercising without fluid and from 283 (2.0) to 291 (0.7) mosmol.kg-1 (p < 0.02) in subjects taking oral rehydration solution. No significant increases were observed when taking water only. CONCLUSIONS: During dehydration, total body water loss was derived from both fluid compartments but extracellular water contributed the most. Effective rehydration depends on adequate replacement of electrolytes lost from each fluid compartment. Water alone may not provide adequate rehydration.

Adult↗
PubMed1993

Role of the sympathetic nervous system in the generation of one-minute wave in body fluid volume during upright standing.

In clarifying the role of sympathetic nerve outflow to the muscles in the genesis of one-minute waves in body fluid volume, power spectral analysis of muscle sympathetic nerve activity (MSNA) was performed. MSNA was recorded by microneurography in an upright standing position simultaneously with the measurements of body circumference, venous pressure at the dorsum pedis veins, intrathoracic fluid volume, soleus EMG, and calf fluid volume. Subjects were seven healthy male volunteers aged 18 to 32 years. Spectral analysis revealed the one-minute oscillation in MSNA as well as in other variables. Cross spectral analysis between the soleus EMG and each variable revealed a phase order of the one-minute oscillations. It was as follows: 1) an increase in calf fluid volume; 2) a decrease in intrathoracic fluid volume; 3) an increase in venous pressure at the dorsum pedis veins; 4) an increase in the soleus EMG activation; 5) an activation of MSNA; 6) a decrease in calf fluid volume; and 7) an increase in intrathoracic fluid volume. It is concluded that sympathetic nerve activity as well as cardiovascular variables have a cyclic rhythm with a duration of one minute to maintain hemodynamic homeostasis in humans in an upright standing position.

Adolescent↗
PubMed1999

Liquid chromatographic determination of ethyl alcohol in body fluids.

A high-performance liquid chromatographic technique for ethyl alcohol determination in body fluids is proposed. Ethyl alcohol is quantitatively converted into acetaldehyde-phenylhydrazone by oxidation in the presence of alcohol dehydrogenase, nicotinamide-adenine dinucleotide and phenylhydrazine. The derivative is suitable for reversed-phase liquid chromatography and ultraviolet detection at 276 nm. The limits of linearity, detection and quantification as well as accuracy and reproducibility were investigated in water, serum and whole blood. Analytical responses were linear within the 0.008 to 5 g/l range, and the limit of quantification was 0.02 g/l both in aqueous standard and in biological matrix assays. Mean analytical recovery of ethyl alcohol in blood serum averaged 98.2+/-4.2%, imprecision (CV%) at 0.80 g/l was 2.2%, and the limit of quantification was 0.02 g/l. Serum concentrations of persons that avoided alcoholic beverages for a week were less than the limit of quantification. Ethyl alcohol concentrations in serum and whole blood compared well with those obtained by headspace gas chromatography. This simple and reliable procedure, which was also used for a urine assay, could be suitable for validation of the screening procedures used to monitor ethanol abuse.

Chromatography, High Pressure Liquid↗
PubMed1974

A method for quantitating amylase and its use in the investigation of various body fluids.

A plate-diffusion technique for the quantitative assay of amylase in body fluids is described which is dependent on the incorporation of a soluble amylopectin-dye complex into agar. The technique correlates well with other established amylase assays, and has a coefficient of variation of 1.45 for amylase levels within the normal range for serum. Normal levels of amylase in serum and urine are reported which agree with those recorded by previous workers, and in addition values of amylase in semen, saliva, and tears are quoted.

Amylases↗