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Recombinant human osteogenic protein-1 induces dentin formation: an experimental study in miniature swine.

It was the aim of the present study to investigate the induction of dentin formation by recombinant human osteogenic protein-1 (hOP-1). In 4 adult miniature pigs a total of 16 teeth with artificially exposed dental pulps were capped with 3 mg of a complex of recombinant hOP-1 in collagen matrix (2.5 micrograms/mg), collagen matrix alone, or calcium hydroxide paste. Teeth were removed in block section after a healing period of 5 weeks. Decalcified sections were processed for light microscopy and histomorphometric analysis. In hOP-1 treated teeth substantial amounts of hard tissue formation (osteodentin and tubular dentin) had consistently led to a complete bridging of the defects. Less dentin formation was seen after calcium hydroxide application. In control defects collagen matrix alone failed to form complete dentin bridges. Recombinant human osteogenic protein-1 in a collagen carrier matrix appeared to be suitable as a bio-active capping agent for surgically exposed dental pulps.

Animals↗

The CO2 laser as an aid in direct pulp capping.

Two hundred direct pulp capping procedures were conducted in the present study. One hundred of them were performed with the CO2 laser, and 100 were conducted conventionally as a control by using a calcium hydroxide preparation. Follow-up examinations were performed after 1 wk and monthly for 12 months after treatment. Thermal tests were used for vitality assessments and laser Doppler flowmetry for direct measurement of pulpal blood. In the group of pulps treated with the CO2 laser, the last recall examination at 12 months demonstrated that 89 teeth remained vital, corresponding to a success rate of 89%. In the control group, the success rate was considerably lower (68%). Exposure sizes and mean patient age were nearly identical in both groups. The CO2 laser seems to be a valuable aid in direct pulp capping.

Adolescent↗

Overviews of pediatric-endodontics.

Pediatric-endodontics is an expanding part of pediatric dental practice. Emphasis is centered on the retention of primary teeth until the normal exfoliation time utilizing indirect pulp therapy, pulpotomy, pulpectomy and root canal fillings. The preservation of vitality in young permanent teeth via pulp capping and pulpotomy is paramount until root formation is completed. When pulpless, these young teeth may be salvaged by apexification procedures.

Child↗

Feline endodontics.

One misconception in veterinary medicine is that fractured teeth do not need to be treated, or treatment should consist of extraction only. Two common causes of endodontic disease in the cat are fractures and secondary to cervical line lesions. Endodontic technique can be useful in returning a diseased tooth to normal, pain-free function. Conventional endodontics can be used to treat fractured canine teeth or partially destroyed molars. Surgical endodontics is useful in cases in which an apical seal cannot be established by conventional methods. Pulp capping can preserve a vital tooth as an alternative to extraction.

Animals↗

New restoration and direct pulp capping systems using adhesive composite resin.

There have been many arguments on the irritating mechanisms of the composite resin on the dental pulp. While the direct irritative effect of the resin has been preferred, some authors considered that the marginal microleakage and the resulting bacterial infection play a more important role in inducing the complicating pulp irritation. We developed a new filling technique, called the direct inlay restoration method, which could prevent the marginal leakage associated with the polymerization shrinkage of the adhesive composite resin. In this study, we tried to apply our method clinically. None of the 440 cases which were filled with the adhesive composite resin and 60 cases out of 64 cases in which the pulps were directly capped with the adhesive composite resin developed any signs and symptoms of pulp irritation. The other 4 cases developed signs of pulp irritation. Two of those 4 cases were pulpectomized due to spontaneous pain and the other 2 cases turned out to be well after re-restoration. With the informed consent of the patients, the direct pulp capping using the adhesive composite resin was experimentally performed on 6 caries-free 3rd molars and the histopathological examination of these capped molars revealed that neither significant degenerative nor inflammatory changes were brought about in the dental pulp. These clinical and histopathological observation suggest that the dental pulp irritation after resin filling is not induced by the composite resin itself.

Adolescent↗

Placement of cast restorations over direct pulp capping procedures: a decision analytic approach.

A review of the literature shows a significant amount of controversy regarding the success of pulp capping procedures, with the average clinically evaluated pulp capping success being 81%, and 75% when evaluated histologically. This study evaluated the costs and benefits of a pulp cap versus endodontic therapy for a tooth that is to receive a cast restoration, and determined what level of success is needed for pulp capping to be the preferred treatment.

Adult↗

Ultrastructure of initial calcification on exposed human pulp applied with autogenous dentin fragments.

Initial calcification in human dental pulp has been studied with electron microscopy in 20 human teeth over a period of 21 days following pulp exposure and capping with autogenous dentin fragments. Five days after operation, dentin fragments were surrounded by degeneration cells and inflammatory cells. Occasionally a macrophage that had phagocytosed a dentin fragments was observed. Fourteen days after operation, osmiophilic needle-like crystalline structures were found in spherical bodies in the collagen fibrils or on the dentin surface. Ca and P were detected in these needle-like crystalline structures by energy dispersive X-ray analysis. They tended to increase in size and to fuse together to form mineralizing globules with the passage of time. Initial calcification around the dentin fragments developed various patterns, including spherical bodies in the collagen fibrils and direct apposition of needle-like crystals on the dentin fragments.

Adolescent↗

[Using mineral trioxide aggregate as a direct pulp-capping material in dogs].

OBJECTIVE: The purpose of this study was to compare the dental pulp responses in dogs after MTA (Mineral Trioxide Aggregate) and a calcium hydroxide (Dycal) were applied as pulp-capping materials. METHODS: Twelve cuspids in three healthy adult beagle dogs were divided into two groups randomly. MTA or Dycal was used to repair the exposed site which was created with a fissure bur in a high-speed handpiece with copious water spray at the labial cervical access. Then the cavity was filled with glass ionomer cement (GIC). Four months later, the specimens were processed for histologic examination to observe the pulpal inflammation and dentin bridge formation. RESULTS: Five of six samples capped with MTA were free of pulpal inflammation, and all of samples in this group had dentin bridge formation. In contrast, all of samples capped with Dycal showed pulpal inflammation, and dentin bridge formation occurred in only 2 samples. CONCLUSION: MTA is a biocompatible material which can promote dentin bridge formation. It can be used as a direct pulp-capping material during vital pulp therapy.

Aluminum Compounds↗

Evaluation of techniques and material used in pulpal therapy based on a review of the literature: part II.

A healthy and functional dental pulp is the goal of any treatment procedure, but at present there does not seem to be any material that will regularly induce dentinal bridge formation and healing of an exposed, inflamed pulp. The usefulness of various capping agents, including corticosteroids and isobutyl cyanoacrylates, is interpreted. In determining treatment, all the clinician's experience is needed in correlating clinical evidence, history, size of the filling in relation to the tooth crown and periodontal condition.

Adrenal Cortex Hormones↗