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At least 685 records · Page 38Linked to original sources
PubMed2000

Sperm transport in the female reproductive tract of the brushtail possum, Trichosurus vulpecula, following superovulation and artificial insemination.

This study investigated sperm transport following superovulation and artificial insemination (AI) in the common brushtail possum, Trichosurus vulpecula. Females were superovulated by treatment with 15 IU pregnant mare serum gonadotrophin (PMSG) then 4 mg luteinizing hormone (LH) 78 h later. Inseminations were performed 27 h after LH (4 million motile spermatozoa/uterus). At 1.5, 3, 6, 9 and 12 h after AI (n=5 per group), females were euthanised and reproductive tracts removed for examination and flushed for sperm. No ovulations had occurred by 1.5 h, but 20% of animals had ovulated by 3 or 6 h, and 80% by 9 or 12 h. The mean numbers of spermatozoa recovered ranged from 249 to 275x10(3) in the uterus; 16-51x10(3) in the isthmus; 8-11x10(3) in the middle segment; and 6-16x10(3) in the ampulla at 1.5, 3 and 6 h after AI. Sperm numbers in all regions decreased at later times (P<0.05) except the isthmus, where 100x10(3) sperm were recovered by 12 h. Highly motile thumbtack sperm (a putative indicator of capacitation in marsupials), were recovered from the isthmus (20%), middle segment (50%) and ampulla (90%) at all sampling times, but not from the uterus. The epithelium of the oviduct segments contained mucus-secreting and ciliated cells and peak secretory activity was observed in the ampulla at 6 h. At 3, 6 and 12 h, many spermatozoa were found in epithelial folds within the isthmus. The present study has provided basic information on sperm transport and storage events within the female reproductive tract of T. vulpecula following superovulation and AI. It is concluded that this model may be useful to better understand pre-fertilization sperm maturation events in the possum, which could facilitate the development of IVF technology.

Animals↗
PubMed2003

Timeliness and effectiveness of progeny testing through artificial insemination.

Progeny-test (PT) programs of US artificial-insemination (AI) organizations were examined to determine timeliness of sampling, PT daughter distribution, rate of return of PT bulls to widespread service, and genetic merit of PT bulls compared with AI-proven and natural-service (NS) bulls. Bull age at semen release and at birth and calving of PT daughters was documented by breed (Ayrshire, Brown Swiss, Guernsey, Holstein, Jersey, and Milking Shorthorn) for bulls that entered AI service since 1960. Mean Holstein bull age at semen release (16 mo) changed little over time, but standard deviations (SD) decreased from 4.0 mo during the 1960s to 2.4 mo during the 1990s. Most Holstein bulls (80%) had semen released by 18 mo. Mean age of Holstein bulls at birth and calving of PT daughters during the 1990s was 29 and 56 mo, respectively (a decline of 4 mo from the 1960s); SD decreased from 6 to 3 mo. Bulls of other breeds usually were older at birth and calving of PT daughters, and SD were larger. Mean Holstein bull age when 80% of PT daughters had been born declined from 36 mo during the 1960s to 31 mo during the early 1990s; for other breeds, bulls showed the same trend but at older ages. Mean Holstein bull age when 80% of PT daughters had calved declined from 65 mo during the 1960s to 59 mo during the 1990s; for other breeds, bulls were older. Percentage of herds with PT daughters has increased over time. For Holsteins, herds with five or more usable first-parity records that had PT daughters with usable records increased from 15% during 1965 to 61% during 1998; percentage of herds with from 1 to 19% PT records increased from 11 to 38%, and percentage of herds with >50% PT daughters increased from 1 to 5%. Percentage of Holstein PT bulls returned to AI service declined to about 12% for bulls with PT entry around 1990; for other breeds, 12 to 23% of most recent PT bulls were returned to service. Percentage of milking daughters that had records usable for genetic evaluation that were sired by PT bulls increased steadily from 10 to 18%, whereas percentage of daughters with usable records that were sired by NS bulls declined from 14 to 7%. Milk yield of daughters of AI-proven bulls was 107 to 200 kg greater than for daughters of PT bulls and 366 to 444 kg greater than for daughters of NS bulls for all years. More extensive and rapid sampling and increased selection intensity of PT programs have led to more rapid genetic progress. More extensive use of AI could increase US producer income by millions of dollars annually.

Aging↗
PubMed2005

Pregnancy rates and milk production in natural service and artificially inseminated dairy herds in Florida and Georgia.

Effects of artificial insemination (AI) and natural service (NS) breeding systems on pregnancy rates (PR) by stage of lactation, season, and changes in milk production over time were examined using lactation and herd DHIA records of Holstein cows in dairy herds located in Florida and Georgia. The reported genetic profile of service sires of the herd was used to determine the percentage of cows bred to natural service bulls (%NS). Two seasons were considered: winter (November-April) and summer (May-October) from 1995 to 2002 (16 periods). Herd-periods were assigned 1 of 3 breeding systems: AI (0 to 10% NS), mixed (11 to 89% NS) and NS (90 to 100% NS). Seventy percent of the herds used NS bulls as a component of their breeding system during the study period. The PR during winter (17.9%) was greater than that during summer (9.0%). During winter, PR for AI herds (17.9%) did not differ from that for mixed (17.8%) and NS herds (18.0%). During summer, PR for AI herds (8.1%) was slightly less than that for mixed (9.3%) and NS herds (9.8%). During winter, PR for cows at 71 to 91 d, 92 to 112 d, and 113 to 133 d in milk were 1.4% lower for mixed herds compared with AI and NS. Pregnancy rate for NS herds was 2.6% lower during late lactation compared with AI and mixed herds. During summer, PR for cows at 71 to 91 and 92 to 112 d in milk were 2.6 and 1.8% greater, respectively, for NS herds compared with AI. However, from 260 to 364 d in milk, PR for NS herds was less than that for AI and mixed herds. No significant interaction was detected between breeding system and lactation number. Rolling herd average milk production during the study period was less in the NS herds (7180 kg) compared with AI (8513 kg) and mixed herds (8176 kg), but the annual change in milk production was not different among breeding systems. The results indicated that use of NS bulls did not result in meaningful disadvantages in terms of PR and changes in milk production over time.

Animals↗
PubMedDate not supplied

Determination of optimal time for mating by artificial insemination with chilled semen using luteinizing hormone surge as an indicator in beagles.

Artificial insemination (AI) was conducted using the second fraction of semen, which was collected from 15 male dogs, diluted to a total sperm count of 100x10(6) for each insemination with egg-yolk Tris (eyT) citrate acid buffer and incubated at 4 degrees C for 48 hours. Luteinizing hormone (LH) surge was detected to determine the optimal time for mating using canine LH assay kits. Artificial insemination using 100x10(6) sperm was performed on the fourth and sixth days or the fifth and seventh days after the LH surge. The conception rates were 33% (4/12) and 89% (8/9), respectively; the whelping rates also showed similar results. Serum LH and follicle stimulating hormone (FSH) concentrations were measured in nine dogs, and the mean LH concentration (+/- standard deviation) at LH surge was 15.77+/-7.66 ng/ml. The time of the LH surge detected by the canine LH assay kit was very similar to that measured by radioimmunoassay (RIA).

Animals↗
PubMed2003

Seminal lead concentrations negatively affect outcomes of artificial insemination.

OBJECTIVE: To determine the relationships among seminal lead levels, acrosome status, and artificial insemination cycle fecundity (AI f) in semen donors. DESIGN: Longitudinal analysis of seminal lead levels, sperm function testing, and fecundity. SETTING: University medical center andrology and research laboratories. PATIENT(S): Semen donors (n = 15) participating in a therapeutic donor insemination program. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Seminal plasma lead levels, acrosome sensitivity to progesterone (P) and voltage-gated potassium channel inhibitors (e.g., charybdotoxin [CBTx]), and AI f. RESULT(S): Seminal plasma lead levels and AI f were strongly negatively correlated. Semen donors were divided into three groups by acrosome response to P: normal (CBTx sensitive [Rs] or CBTx resistant [Rr]: responders) and reduced (nonresponders [NR]) (Rs > Rr >> NR). Seminal lead differed among the three groups (NR > Rr > Rs). Comparison of 330 artificial insemination cycles from four Rs, four Rr, and two NR demonstrated that cycle AI f also differed significantly between groups (Rs >Rr >>NR). CONCLUSION(S): Measurements of seminal plasma lead, P-stimulated acrosome loss, and sensitivity to CBTx may provide prognostic information on the fertility status of potential donors as well as male infertility patients. Such evaluations may assist in donor acceptance, or in the case of patients, in selection of the appropriate treatment regimen.

Acrosome Reaction↗
PubMed1988

Legislation on artificial insemination, 1 May 1988.

On 1 May 1988, the governing body of the canton of Glarus, Switzerland, voted that homologous artificial insemination would be the only assisted reproduction procedure authorized to be performed in the canton. On 15 March 1989, the Swiss Bundesgericht declared prohibitions on artificial insemination by donor and homologous in vitro fertilization to be unconstitutional. This decision will be reported in Annual Review of Population Law, Vol. 16, 1989.

Constitution and Bylaws↗
PubMed1976

A revised artificial insemination schedule for broiler breeder hens.

Broiler type hens maintained in individual cages were artificially inseminated with either 0.023, 0.035, or 0.047 ml. of pooled semen. Insemination intervals were nine, nine, and ten days on a repetitive basis. This schedule was adopted because it allowed an extension of the conventional insemination interval and yet remained compatible with a five-day industrial work-week. The results demonstrated that maintaining broiler breeder hens in cages and utilizing artificial insemination as a means of obtaining fertile eggs were feasible. The data also indicated that extending the insemination interval to 10 days is possible providing the number of spermatozoa inseminated is increased.

Animals↗
PubMed2006

GnRH-agonist induction of fertile estrus with either natural mating or artificial insemination, followed by birth of pups in gray wolves (Canis lupus).

Although captive populations of endangered species such as the Mexican gray wolf (Canis lupus baileyi) can benefit from artificial insemination to accomplish genetic exchange, reliable techniques for timing insemination are lacking. We used the generic gray wolf (C. lupus) to test the efficacy of a short-acting GnRH-agonist implant, deslorelin, for inducing estrus. Of five females receiving implants on 17 or 18 January 2003, two mated naturally 10-17 days later, and the others were artificially inseminated using fresh semen, one on day 7 and all three on day 11. Relaxin tests revealed that one artificially inseminated female and both naturally mated females were pregnant on 1 March, and all three gave birth to healthy puppies on 4-6 April. Of the artificially inseminated females, only the one who subsequently conceived and gave birth was judged to be in cytologic estrus at the time of insemination. Two females were treated again with deslorelin on 12 January 2004, followed by collection of fecal samples for hormone analysis. One female, who was housed with a male, copulated on day 17 but did not conceive; the other was not with an adult male. Fecal progestin and estrogen profiles suggested that estrus, but not ovulation, was induced. These results indicated that deslorelin could induce fertile estrus in the gray wolf, although individual response varied. Further investigation is needed to better define and control the interval between implant insertion and ovulation for optimal timing of insemination.

Animals↗
PubMed1982

Psychological and legal aspects of artificial insemination (A.I.D.): an overview.

The psychological and psychodynamic determinants and end-products of A.I.D., both of a positive and negative nature, are reviewed. Studies do not support the contention that a decision to participate in artificial insemination, in itself, is indicative of an emotional disturbance. There is no justification for the automatic requirement of psychological or psychiatric examinations of couples requesting this procedure. Some legal issues are reviewed.

Adaptation, Psychological↗
PubMed1985

Treatment of idiopathic infertility, cervical mucus hostility, and male infertility: artificial insemination with husband's semen or in vitro fertilization?

Couples with male infertility (n = 86), idiopathic infertility (n = 68), and cervical mucus hostility (n = 48) of a long duration were treated either by in vitro fertilization (IVF) or artificial insemination with husband's semen (AIH). The incidence of pregnancy per cycle in couples in whom the male partner was infertile was significantly (P less than 0.01) higher after IVF, compared with AIH (21% versus 5%, respectively). The differences were most apparent in couples with asthenospermia (47% versus 0%, respectively); no significant difference was found when the infertility was caused by oligospermia only (11% versus 9%, respectively). More patients with idiopathic infertility became pregnant after one cycle with IVF, compared with AIH (20% versus 8%, respectively); but, because of intragroup disparity in size, this difference was not significant. A highly significant difference (P less than 0.01) was found after one attempt with IVF, compared with AIH, in patients with cervical mucus hostility (38% versus 3%, respectively).

Cervix Mucus↗
PubMed2001

An in situ hybridization study of the effects of artificial insemination on the localization of cells expressing MHC class II mRNA in the chicken oviduct.

The aim of this study was to determine the effects of artificial insemination on the localization of antigen-presenting cells expressing MHC class II mRNA in chicken oviducts. Laying hens (35 weeks old) were inseminated with fresh semen or sham-inseminated with saline daily for 3 days. In situ hybridization was performed to detect chicken MHC class II (B-LB21 major gene) mRNA on frozen sections of oviductal infundibulum, uterovaginal junction and vagina by using digoxigenin-labelled PCR probes. Cells expressing MHC class II were observed mainly in the oviductal mucosal stroma and occasionally in the mucosal epithelium. After 24 h, the population of cells expressing MHC class II in the infundibulum was significantly higher in laying hens inseminated with fresh semen than in the control hens sham-inseminated with saline (P < 0.05). However, there was no significant difference in the population of cells expressing MHC class II in the uterovaginal junction and vagina between the artificially inseminated and control hens. These results indicate that anti-sperm immune responses, including the influx of cells expressing MHC class II and enhanced MHC class II mRNA expression, probably occur in the infundibulum after artificial insemination.

Animals↗
PubMed1981

[Menstrual cycle changes during consecutive artificial insemination therapy and their treatment].

Emotional stress-induced problems generated by the application of artificial insemination therapy by donor (AID) are considerable. After 6 months of frustrane insemination treatment, 54% of the women exhibited irregular or anovulatory cycles. Especially the persistent late ovulation often leads to a failure of the mechanisms of conception. An early ovulation timing with high dosages of synthetic LH-releasing hormone or a stimulating LH-RH-analogue (HOE 766) lead to an increasing pregnancy rate after application in the third insemination cycle from 34,0% untreated to 51,1% treated. The early application of ovulation inducing or timing substances is underlined in a program of artificial insemination therapy to eliminate possible stress-induced failures at an early point of treatment. As the spontaneous pregnancy rate is high in the first treatment cycles, LH-RH or its analogue should be administered after two frustrane insemination cycles.

Anxiety↗
PubMed1983

Detection of Campylobacter fetus in artificial insemination bulls with a transport enrichment medium.

One hundred and five bulls from an artificial insemination unit were tested for the presence of Campylobacter fetus subspecies venerealis. The method involved the inoculation of preputial samples into a new transport enrichment medium prior to culture and immunofluorescence tests. Seventeen bulls (16%) were found to be either positive or suspected carriers of C. fetus at one or more sampling times. The average age of these 17 bulls was about two years greater than the average age of all the bulls in the unit. A combined treatment of vaccination and dihydrostreptomycin sulfate injection suppressed or eliminated the organism from carrier bulls. The use of transport enrichment medium has increased our capability and effectiveness to monitor the presence of C. fetus in artificial insemination bulls.

Age Factors↗
PubMed1991

[Artificial insemination from a Catholic viewpoint].

The Roman Catholic Church understands the human propagation as a common act of body and soul, which should not be destroyed either by contraception or artificial insemination. This thesis is based on the natural order and meaning of the unity of man and woman. Within its demands on the state, the church differentiates between artificial insemination of married persons and others.

Catholicism↗