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Mother-child interaction in autistic and nonautistic children: characteristics of maternal approach behaviors and child social responses.

The nature of mother-child interaction in autism and the maternal approach characteristics that elicit social response in children with autism were examined in two studies. Mother-child play sessions of 24 preschool children with autism and 24 typically developing preschoolers were compared in Study 1, and play sessions of 9 mothers with their autistic child and with their nonautistic child were compared in Study 2. Mother-child interactions were coded using the Approach Withdrawal Interaction Coding System to quantify maternal approach behaviors and child responses. Results of Study 1 indicate that, although the quantity of approaches did not differ between mothers with their autistic children and mothers with their nonautistic children, there were qualitative differences. Mothers used more physical contact, more high-intensity behaviors, and fewer social verbal approaches with autistic children. Results of Study 2 replicated these findings with mothers showing a similar pattern of approach toward their autistic children but not their nonautistic children. Although autistic children displayed lower contingency to maternal approaches in general, they showed greater responsiveness to approaches involving increased physical proximity and/or containing nonverbal object use. Mothers socially engaged both autistic and nonautistic children. The implications for parent training and intervention are discussed.

Adult↗

Oxytocin antagonism alters rat dams' oral grooming and upright posturing over pups.

Studies involving intracerebral administration of antiserum or antagonists have demonstrated that central oxytocin (OT) plays a prominent role in initiating but not maintaining postpartum maternal behavior in rats. There has been little investigation, however, of OT's influence on the levels of maternal behavior exhibited during the maintenance phase. We measured rat dam behavior during the 105-min observation periods preceding and beginning 2 h after intracerebroventricular infusion of the selective OT antagonist (OTA) (1 microg), or normal saline (NS) vehicle (5 microl) on postpartum days 2-3 and 6-7. Compared to NS, OTA significantly decreased pup licking as a proportion of dams' total oral grooming, increased self-grooming, decreased the frequency of elevated upright posture over pups and increased the frequency of lying prone on pups. Quiescent, kyphotic nursing was also significantly lower in OTA-treated dams. Other components of maternal behavior were not significantly affected by OTA or NS treatment. These findings suggest that central OT may shift the focus of the dams' oral grooming from self to pups and may also facilitate elevation of dams' upright posture over pups. Acute stress responses, maternal behavior and central OT receptor binding in adult rats have been linked to the amount of maternal licking and arched back, upright nursing received during infancy. OT activity in dams' brains may influence these developmental outcomes in their offspring by selectively regulating their pup licking and crouching posture.

Analysis of Variance↗

Oxytocin links mothering received, mothering bestowed and adult stress responses.

We review recent discoveries that implicate oxytocin in the intergenerational transmission of similar levels of maternal behavior and acute stress responses in female rats. First, ICV-infused oxytocin antagonist decreased the display by nursing dams of pup-licking (PL) and arched-back nursing (ABN), but not other components of maternal behavior, and increased maternal self-grooming suggesting that oxytocin may shift the balance of oral grooming by dams away from themselves and toward pups. Second, oxytocin receptor concentrations in areas of the adult brain where oxytocin stimulates maternal behavior or diminishes anxiety and adrenal axis responses to acute stress were positively related to PL-ABN received during infancy. Third, oxytocin and oxytocin antagonist treatments of pups on postnatal days 2-10, respectively increased and decreased PL by the treated rats when adult and themselves nursing dams. This indicates that oxytocin activity in female pups, which may be regulated by PL-ABN received from their mothers, influences their adult levels of PL. These three lines of evidence suggest that oxytocin selectively enhances PL-ABN by rat dams, which then increases oxytocin activity in female pups and, thereby, facilitates their expression of central oxytocin receptors (and perhaps other aspects of central oxytocin systems) and, consequently, their adult PL-ABN frequencies and acute stress responses.

Animals↗

Effects of oxytocin microinjected into the central amygdaloid nucleus and bed nucleus of stria terminalis on maternal aggressive behavior in rats.

The central effect of oxytocin (OT) on the aggressive behavior of lactating rats was studied. Female rats are more aggressive than nonlactating resident females, vigorously attacking conspecific intruder male or females. This behavior is considered important for pup protection against infanticide. The present work aimed to test the effects on maternal aggressive behavior of OT infused into the central amygdaloid nucleus (CeM) or bed nucleus of stria terminalis (BNST). The surgeries for bilateral cannula implantation were performed between the 2nd and 4th postpartum day. Three days after the surgery, saline or OT was infused and 5 min later a male intruder was placed in the home-cage and the behaviors were videotaped for 10 min. The frequency of the aggressive behaviors and the duration of locomotion during the aggressive behavior test were measured. The latency to retrieve the pups was also evaluated. The results showed that OT injected into CeM (10 and 20 ng/nucleus) decreased frequency of biting and frontal attack while in the BNST (10 and 20 ng/nucleus) decreased the frequency of biting. No significant change on retrieval activity was detected. OT in CeM and BNST has an inhibitory effect on the aggressive behavior of lactating female rats.

Aggression↗

Does traditional birth attendant training improve referral of women with obstetric complications: a review of the evidence.

This narrative and meta-analytic review of the effectiveness of traditional birth attendant (TBA) training to improve access to skilled birth attendance for obstetric emergencies produced mixed results. Among 16 studies that fit the inclusion criteria, there is a medium, positive, non-significant association between training and TBA knowledge of risk factors and conditions requiring referral; and small, positive, significant associations between TBA referral behavior and maternal service use. These results cannot be causally attributed to TBA training because of the overall quality of studies; moreover, in several studies TBA training was a component of integrated intervention packages. The effort and expense of more rigorous research focusing on TBA training to improve access to emergency obstetric care are difficult to justify. The referral process is complex; the real effects of TBA training on TBA and maternal behavior are likely to be small; and while the proportion of TBA-attended births worldwide varies, it is, on average, quite low. The behavioral determinants and logistical barriers to care seeking for emergency obstetric care are generally well known. We suggest a more promising research agenda would reposition the questions surrounding referral into a broader ecological perspective.

Evidence-Based Medicine↗

Perinatal complications and child abuse in a poverty sample.

OBJECTIVE: Perinatal medical illness has been associated with child maltreatment. Using a Child Protective Service (CPS) report as the defining event, this study explores to what extent perinatal morbidity is a risk factor for maltreatment. METHOD: Medical charts of 206 children ages 0-3 years were reviewed. Data regarding birth history were collected and analyzed in three groups of children: children whose medical record indicated a report to CPS based on prenatal findings (Early Maternal Inadequacy group [EMI]), children whose medical record indicated a report to CPS based only on postnatal findings (Child Maltreatment group [CM]), and a control group without CPS report (NM). RESULTS: Compared to the CM and the NM groups, children in the EMI group showed significantly lower birth weight and higher neonatal morbidity as measured by Apgar scores, frequency of oxygen requirement and intubation at birth, frequency of admission to Neonatal Intensive Care unit, and frequency of neonatal medical problems. There was no significant difference between the CM and the NM groups in birth weight, gestational age, and other measures of morbidity. CONCLUSION: The results of the study suggest that perinatal complications are associated with prenatal maltreatment. Previously reported strong associations between neonatal morbidity and child abuse are more likely a result of antecedent prenatal maternal behaviors (early maternal inadequacy). Early maternal inadequacy, a clinically and demographically distinct phenomenon, is important due to serious health, development and financial implications and deserves further exploration.

Birth Injuries↗

Didelphid behavior.

There are approximately 75 species of American opossums (didelphids). Systematic behavioral research has been reported for only a small number of species, namely, Monodelphis domestica and Didelphis virginiana. Observational studies and field reports on several other didelphid species have also been published. The current review covers behavioral studies on didelphids including research on territoriality, reproductive behaviors, parturition, maternal behaviors, nest-building, vocalizations, scent-marking, circadian rhythms, swimming, locomotion, discrimination learning, maze learning, operant learning, problem box learning, exploration, death-feigning, predatory behavior, and prey manipulation. Didelphid behavior is compared in several contexts with eutherian mammals. Other than death-feigning, which has only been reported for one or two didelphid species, the behaviors observed in didelphids are qualitatively similar to those of eutherian mammals. Nevertheless, differences can be seen in prey manipulation and spatial maze behavior. Social interactions appear to be sparser in opossums than in most eutherian mammals. However, the scarcity of systematic research on didelphid behavior still prevents firm conclusions regarding these ancient and interesting mammals. Futher research on didelphids is warranted, particularly since they provide a natural comparison group for small generalist eutherians.

Animals↗

Brain substrates of infant-mother attachment: contributions of opioids, oxytocin, and norepinephrine.

The aim of this paper is to review recent work concerning the psychobiological substrates of social bonding, focusing on the literature attributed to opioids, oxytocin and norepinephrine in rats. Existing evidence and thinking about the biological foundations of attachment in young mammalian species and the neurobiology of several other affiliative behaviors including maternal behavior, sexual behavior and social memory is reviewed. We postulate the existence of social motivation circuitry which is common to all mammals and consistent across development. Oxytocin, vasopressin, endogenous opioids and catecholamines appear to participate in a wide variety of affiliative behaviors and are likely to be important components in this circuitry. It is proposed that these same neurochemical and neuroanatomical patterns will emerge as key substrates in the neurobiology of infant attachments to their caregivers.

Animals↗

[Transmission of binding by mothers and their children in the preschool age].

A new method is presented in order to assess attachment representations in preschoolers. Using the symbolic medium of doll play and story completions the internal representations of attachment in children beyond infancy can be assessed. Additionally the thesis of transmission of attachment across generations was tested empirically in a sample of 28 German families from middle class socioeconomic background as part of longitudinal study. Transmission of attachment means that parents transmit their different attachment representations to preschool-aged children. Secure and insecure attachment representation in mothers assessed with the Adult Attachment Interview, are associated systematically with their childrens' attachment representations coded from the doll play. In the present study the Adult Attachment Interview was conducted with mothers during the fifth year post delivery. Their first-born children, 17 boys and 11 girls with a mean age of 6;4 years participated in the story completion session. Using a two-fold distinction (secure and insecure) as well as a converted 4-level scale of attachment security, results revealed a correspondence between maternal and child attachment representations. According to psychometric indicaters the story completion procedure demonstrates satisfying reliability and convergent validity with the Separation Anxiety Test. Finally, reasons for the high match of mothers' and childrens' attachment representations are discussed. Maternal sensitive behavior, maternal reflection of their own childhood triggered by the observation of their own child, and various learning processes may operate as mediating factors in this case.

Adult↗

Commentary: is maternal stimulation the mediator of the handling effect in infancy?

In a recent report, a series of studies is described showing that individual differences in rat maternal licking and grooming are correlated with their offsprings' later adrenal response to a stressor (Liu et al., 1997). Pups that received more maternal stimulation in infancy had lower ACTH, corticosterone, and CRH mRNA, while they had greater amounts of GR mRNA. Liu et al. also compared maternal behavior in litters where rat pups were handled daily to maternal behavior of nonhandled litters. They found that mothers of handled pups licked and groomed their young significantly more often than did mothers of control litters. In their discussion, Liu et al. proposed that their data support Levine's (1975) thesis that handling effects in infancy are mediated via the mother. Levine had proposed that handling of pups will modify the mother--pup interaction, thereby causing a change in maternal behavior. The purpose of this commentary is to summarize data obtained with mice and rabbits which, in part, support and, in part, challenge the conclusions of Liu et al.

Adaptation, Physiological↗

MBR: a computer program to record and analyze parental behavior in rodents.

The Maternal Behavior Recorder (MBR) is a microcomputer program designed to record and analyze data from research on parental behavior of rats and other rodents. It is used to record the specific, characteristic patterns of maternal care in rodents: nest building, grooming, licking, crouching, and retrieval of pups. Moreover, it analyzes these events in terms of frequency and duration, allowing any events erroneously recorded to be corrected. The MBR can also simultaneously control observations made by one or two experimenters, and it calculates a set of reliability measures between them.

Animals↗

Vaginal stimulation: an important determinant of maternal bonding in sheep.

The immediate induction of the full complement of maternal behavior in nonpregnant ewes primed with estrogen and progesterone has been obtained after 5 minutes of vaginal-cervical stimulation. A similar period of such stimulation given to recently parturient ewes, after the development of selective bonding to their own lambs, reversed their rejection behavior of alien lambs and produced a state of plasticity in maternal behavior, such that ewes receiving vaginal stimulation would accept and adopt alien lambs. These findings implicate vaginal-cervical stimulation as playing a role in the onset of maternal behavior.

Animals↗

Atlas of the neurons that express mRNA for the long form of the prolactin receptor in the forebrain of the female rat.

Prolactin has a variety of important physiological effects on peripheral tissue and on the brain. The behavioral effects of prolactin include the induction of maternal behavior and increased food intake. Prolactin acts via its cognate receptors which have two forms, a short and a long form. The long form of the receptor is predominant in the preoptic area-hypothalamus and is positioned to support maternal behavior since this form is regulated across pregnancy and lactation (Nagano and Kelly [1994] J. Biol. Chem. 269:13337-13345; Sugiyama et al. [1994] J. Endocrinol. 141:325-333). By using in situ hybridization with [33P] labelled cRNA probe specific for the long form of the receptor mRNA(L-PRL mRNA) we have mapped, in brains from 2- and 21-day-old pregnant females, the neuroanatomical distribution of neurons expressing the long form of the receptor. Many neurons with high expression of L-PRL mRNA were located in the anteroventral periventricular nucleus, the medial preoptic area (MPO), specific subdivisions of the paraventricular and supraoptic nuclei, and in the arcuate and ventromedial nuclei. Labelled neurons were also found in limbic system structures such as the bed nucleus of stria terminalis (BST) and the medial nucleus of the amygdala, in a few thalamic nuclei, and in the central gray. All cells throughout the choroid plexus expressed high levels of L-PRL mRNA. The levels of L-PRL mRNA were higher in females on day 21 of pregnancy in the MPO and in the choroid plexus, than in females on day 2 of pregnancy; levels in the ventromedial nucleus of the hypothalamus (VMH) were unchanged across pregnancy. The neuroanatomical distribution of neurons expressing L-PRL mRNA may have special relevance for the mediation of maternal behavior, lactation, sexual behavior, and feeding.

Animals↗

Tendency to display spontaneous incubation does not affect maternal responsiveness in the domestic hen.

Induction of maternal behavior was performed in 63-week-old hens of the JV15 (or "Vedette") strain which had previously exhibited brief incubating phases during the preceding egg-laying period to discover if their maternal behavior differed from that induced in hens who had no previous record of incubating. The degree of maternal behavior exhibited showed large variation among individuals. However, hens with a history of one or more spontaneous, brief incubation bouts and hens without such histories showed similar responsiveness toward chicks and similar levels of general activity. In most of the subjects, the establishment of maternal behavior was accompanied by a significant long-term fall in the plasma levels of luteinizing hormone whereas the plasma levels of prolactin appeared to be unaffected.

Animals↗

[Sensory stimuli in the reproductive function].

There is very ancient evidence that sensory systems and specific stimuli affect reproductive functions. Studies of the reproductive cycles and behavior led to the concept that simuli acting through the nervous system could affect the endocrine system. The event which normally induces the milk ejection reflex is stimulation of the teat; however under certain conditions the reflex can be provoked by other sensory stimuli as the sound of other rats nursing their litters. The endocrine mechanisms involved in the termination of pregnancy in rats seem to be essential for the occurrence of parturition, the expression of maternal behavior and lactation. Ovariectomy performed on day 20 of pregnancy impaired parturition, maternal behavior and lactation. Treatment with estrogen permitted normal delivery, maternal response and lactation only when the hormone was injected immediately after ovariectomy. Estrogen administered the day after was not effective. The administration of progesterone to ovariectomized pregnant rats prevented parturition. Maternal behavior did not occur in ovariectomized-progesterone treated rats when delivery was induced by Caesarean section on day 22 of pregnancy. The association of a progesterone decrease and a simultaneous estrogen secretion is an important step for normal parturition, maternal behavior appearance and normal lactation. After parturition a progressive increase in circulating progesterone seems to "adapt" the C.N.S. to the steroid which may modulate the release of prolactin induced by suckling. An acute increase of the progesterone level by s.c. silastic implant or injection during the first 3 or 6 days following parturition impairs the normal growth of the litter probably by impairing milk secretion and milk ejection. Prolactin release was significantly diminished by the progesterone treatment. Pretreatment with an antiestrogen potentiates the inhibitory effect of progesterone on litter growth.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Effects of naloxone on newborn infant behavior after maternal analgesia with pethidine during labor.

Infants born to mothers receiving 100 mg of pethidine during labor, were randomly given either 100 micrograms of naloxone (n = 14) or 0.25 ml 0.9% NaCl (n = 13) one hour post partum. Infant behavior was assessed with the Brazelton Neonatal Behavioral Assessment Scale (BNBAS) and the Broussard Neonatal Perception Inventory (NPI). No differences in cluster scores on the BNBAS were found between the two groups. Both groups improved scores over time in 4 out of 7 clusters. On the NPI, mothers assessed naloxone infants as having less optimal behavior than did the control mothers. The results of this study on the effects of naloxone on infant behavior and maternal perception of newborn behavior do not warrant administration of naloxone after maternal analgesia with pethidine in the absence of clinical evidence of respiratory depression in the newborn.

Adult↗

FOS and FOSB expression in the medial preoptic nucleus pars compacta of maternally active C57BL/6J and DBA/2J mice.

C57BL/6J and DBA/2J inbred mice differ in aspects of maternal behavior and in the morphology of the medial preoptic nucleus (MPO), suggesting a possible association. DBA/2J mice have a compact subnucleus in the MPO, the MPOpc, that is sexually dimorphic and absent in C57BL/6J mice. To determine whether MPOpc cells are activated by maternal behavior, FOS and FOSB immunohistochemistry was performed on brain sections of C57BL/6J and DBA/2J mothers following the return of their pups after a separation of 2 days. In both light and dark phases of the daily cycle, stimulation of DBA/2J mothers evoked an increase in FOS- and FOSB-immunoreactivity in the MPOpc. Stimulated C57BL/6J mice, which lack the MPOpc, did not show an increase in cellular activity in the corresponding MPO region. Cells immediately lateral to the MPOpc were activated by pup stimulation, in both strains. These results suggest that MPOpc cells are active during maternal behavior, and that strain differences in maternal behavior are related to anatomical differences in the MPO.

Animals↗

Opioid receptor antagonism during early lactation results in the increased duration of nursing bouts.

High levels of mu opioid receptor activation during the postpartum period result in the disruption of ongoing maternal behavior. The role of physiological levels of endogenous opioids on the mediation of maternal behavior in postpartum females, however, has not been closely examined. The purpose of the present experiments was to examine the function of endogenous opioids during early and mid-lactation by treating postpartum females with the opioid antagonist naloxone and monitoring their behavioral interactions with pups. Although this treatment did not lead to any qualitative differences in the maternal behaviors measured (pup retrieval and grooming, nest building, grouping of pups, or crouching over pups), there was a quantitative difference in the amount of time the females spent with pups on the nest and actively nursing pups. Naloxone, given either systemically or centrally (intracerebroventricularly), resulted in prolonged nursing and nesting bouts. This effect, however, was only observed during the early lactation time point (postpartum days 5-7). Females tested later in lactation (postpartum days 10-12 or 12-14) did not display the increased nursing or nesting bouts in response to the antagonist. These data indicate that central opioids play a role in the duration of nursing bouts during early lactation.

Animals↗