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PubMed1996

Lipid peroxidation in workers exposed to manganese.

OBJECTIVES: The following hypothesis was tested: plasma manganese concentration is associated positively with the product of lipid peroxidation, and lipid peroxidation is associated negatively with the activities of antioxidants in workers exposed to manganese. METHODS: The plasma manganese concentration of 22 manganese-exposed workers and 45 referents was determined by graphite furnace atomic absorption spectrophotometry. Malondialdehyde, the product of lipid peroxidation, was determined by high-performance liquid chromatography, and the activities of protective enzymes were measured by ultraviolet-visible spectrophotometry. RESULTS: The activities of superoxide dismutase, glutathione peroxidase, and catalase spread widely among the referents. The activity of superoxide dismutase and the concentrations of malondialdehyde and manganese were significantly higher in the manganese workers than in the referents. The concentration of malondialdehyde in the exposed workers was correlated strongly with the manganese level of plasma. CONCLUSIONS: Malondialdehyde can be used as an index of lipid peroxidation induced by manganese exposure.

Antioxidants↗
PubMed1982

Simple monitoring of low salicylate concentrations in capillary blood. An appraisal of ultraviolet spectrophotometric methods.

The differential ultraviolet spectrophotometric determination of salicylate according to Williams et al. (11) and the single extraction ultraviolet spectrophotometric method of Stevenson (9) are subject to interference by serotonin. Probably as a result of this interference, the results are erratic when low concentrations of salicylate are monitored in capillary blood. Owing to a peculiarity of the ultraviolet absorbance spectrum of serotonin, there is no appreciable interference by serotonin, if the double extraction method of Stevenson (9) is used, in which the spectrophotometry is carried out in phosphate buffer of pH 6.86. With due precautions and standardization, reliable results are obtained, so that this comparatively simple and rapid method can be recommended wherever capillary blood sampling (finger tip, the heel in pediatrics) is preferable to venous blood sampling. Pharmacokinetical results, obtained by this method, are given for three human subjects after a single oral dose of 500 mg aspirin.

Adult↗