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At least 685 records · Page 38Linked to original sources
PubMed2005

Laboratory colonization of Aedes lineatopennis.

Aedes lineatopennis, a species member of the subgenus Neomelaniconion, could be colonized for more than 10 successive generations from 30 egg batches [totally 2,075 (34-98) eggs] of wild-caught females. The oviposited eggs needed to be incubated in a moisture chamber for at least 7 days to complete embryonation and, following immersion in 0.25-2% hay-fermented water, 61-66% of them hatched after hatching stimulation. Larvae were easily reared in 0.25-1% hay-fermented water, with suspended powder of equal weight of wheat germ, dry yeast, and oatmeal provided as food. Larval development was complete after 4-6 days. The pupal stage lasted 3-4 days when nearly all pupae reached the adult stage (87-91%). The adults had to mate artificially, and 5-day-old males proved to be the best age for induced copulation. Three to five-day-old females, which were kept in a paper cup, were fed easily on blood from an anesthetized golden hamster that was placed on the top-screen. The average number of eggs per gravid female was 63.56 +/- 22.93 (22-110). Unfed females and males, which were kept in a paper cup and fed on 5% multivitamin syrup solution, lived up to 43.17 +/- 12.63 (9-69) and 15.90 +/- 7.24 (2-39) days, respectively, in insectarium conditions of 27 +/- 2 degrees C and 70-80% relative humidity.

Aedes↗
PubMedDate not supplied

[Distribution of trehalose between the cells and the rehydration medium in dehydrated Saccharomyces cerevisiae].

The work was concerned with studying the balance of trehalose distribution between the rehydration medium and Saccharomyces cerevisiae cells grown in a chemically defined medium and dehydrated using the convective technique. A direct linear correlation between the viability of populations and the overall residual trehalose content in the cells and in the medium after the rehydration of dry yeast cells was shown to be most important. An inverse correlation was established between the viability of yeast cells and the amount of trehalose mobilised by the cells in the process of rehydration.

Culture Media↗