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Effect of calcium hydroxide in powder or in paste form on pulp-capping procedures: histopathologic and radiographic analysis in dog's pulp.

The pulps of seventy dog teeth were evaluated histopathologically and radiographically following intentional exposure and treatment with either a calcium hydroxide powder or a calcium hydroxide paste prepared with distilled water. The results were recorded after periods of 2, 30, 70, and 120 days. No differences were detected in the pulpal responses to direct pulp capping achieved with a calcium hydroxide paste and with a calcium hydroxide powder.

Animals↗

Pulp-capping with recombinant human insulin-like growth factor I (rhIGF-I) in rat molars.

The aim of this study was to explore pulp healing and reparative dentinogenesis following pulp-capping by using recombinant human insulin-like growth factor I (rhIGF-I). Exposures were made through the mesial pulp horn in first upper molars in two-month-old Wistar rats. The pulp was covered with one dose of sterile 4% methylcellulose gel containing either 400 ng rhIGF-I or saline in contralateral controls. The exposure site was closed with sterile Teflon membrane, and the cavity was filled with IRM cement. Additional molars were capped with Dycal as controls. After 3, 7, or 28 days, animals were anesthetized and fixed by intravascular glutaraldehyde perfusion. Molars were decalcified and processed for histological analysis and cut with membrane and residual methacrylate from IRM in situ. Only specimens with acceptable pulp sealing according to blinded microscopy control were included. On day 3, identical inflammatory responses in the upper pulp were observed in molars with rhIGF-I gel or control gel. On day 7, granulation tissue ingrowth had partly replaced inflammatory infiltration in both groups. After 28 days, complete dentin bridging and tubular dentin formation were observed more frequently and closer to the test substance containing rhIGF-I. The reparative dentin response to capping with rhIGF-I was similar to that after the use of Dycal. In conclusion, microscopic control of membrane sealing in situ gives valid information on the more subtle pulp effects of growth factors. The observations suggest that pulp-capping of rat molars by means of rhIGF-I enhances reparative dentinogenesis in comparison with vehicle controls.

Animals↗

The diagnosis, classification and treatment of acute pulpal pain by UK general dental practitioners: results of a survey.

There are several problems related to the diagnosis and treatment of the acute painful tooth associated with a deep carious lesion, including the fact that there is little or no correlation between the clinical signs and symptoms and also that the diagnostic tools are not reliable. It is important to understand the problems experienced by dental practitioners and how they routinely make diagnosis and formulate treatment plans. The way general dental practitioners diagnosed, categorised and treated teeth with acute pain was ascertained by a questionnaire completed by practitioners attending postgraduate courses. The results indicate that dental radiographs and the dental history were most frequently used and that few practitioners had, and fewer used electric pulp testers. The concept of using simple clinical categories which correspond to treatment categories does not seem to be in widespread use, though the generally accepted role of calcium hydroxide as an indirect and direct capping material is widespread. The use of corticosteroid materials is not as widespread in younger practitioners as in their older colleagues and the use of emergency pulpotomy is infrequent.

Acute Disease↗

Dens evaginatus from an orthodontic perspective: report of several clinical cases and review of the literature.

The anomaly of dens evaginatus manifests itself as an innocuous-looking tubercle of enamel on the occlusal surface of a premolar tooth. Problems can arise when the tubercle is either worn, ground, or fractured off, resulting in pulpal exposure and possible loss of vitality of the tooth. Orthodontists should be particularly aware of this dental anomaly, which occurs in at least 2% of the Asian and Native Indian population. Premolar extraction cases should be planned to include extraction of the anomalous premolars instead of the normal ones. In addition, the orthodontist should be wary of occlusal changes during treatment or occlusal equilibration that might jeopardize the vitality of teeth with dens evaginatus. Pulp-capping or partial pulpotomy has been postulated as the most reliable form of treatment to prevent loss of vitality of the affected teeth and to allow continued root maturation where necessary.

Adolescent↗

Advantages of a pulsed CO2 laser in direct pulp capping: a long-term in vivo study.

BACKGROUND AND OBJECTIVE: A previous study [Moritz et al., Z Stomatol 1996; 93:451-454] had shown that favourable results in direct pulp capping could be achieved using a continuous wave CO2 laser in addition to the conventional calcium hydroxide dressing technique. In this study, these results are compared to those achieved using a CO2 laser working in superpulsed mode. STUDY DESIGN/MATERIALS AND METHODS: A total of 260 direct pulp capping procedures were carried out; 130 were performed with a superpulsed CO2 laser, followed by a calcium hydroxide dressing, and 130 conventionally by applying only a calcium hydroxide preparation. Recall examinations were performed after 1 week and monthly for 18 months after treatment. A final examination was carried out 2 years after treatment. Thermal tests were used for vitality assessments and laser Doppler flowmetry for direct measurement of pulpal blood flow. RESULTS: In the group of pulps treated with the superpulsed CO2 laser, the last recall examination at 2 years revealed that 93% of the teeth had remained vital. In the control group, the success rate was considerably lower (66.6%). Exposure site sizes and average patient age were nearly identical in both groups. CONCLUSION: The CO2 laser seems to be a valuable aid in direct pulp capping; the efficiency of laser treatment can be increased by using a pulsed CO2 laser.

Adolescent↗

[Clinical, radiological and histological results after vital treatment of exposed pulps].

The authors present the clinical and radiological results of their long-time controls of 210 permanent teeth after direct capping of the exposed pulp and describe some histological findings. The over-all evaluation of the results permits to conclude that the method of direct pulp capping is more successful only if the indication is established discriminately. The patient's age is of no or minor importance to the choice of treatment.

Adolescent↗

Identification of hard tissue after experimental pulp capping using dentin sialoprotein (DSP) as a marker.

The purpose of this study was to identify the hard tissue formed early in experimental pulp exposures capped with mineral trioxide aggregate (MTA) or bone morphogenetic protein (BMP)-7 using dentin sialoprotein (DSP) as a marker. The pulps of 35 maxillary first, second, and third molar teeth from 10 male rats were experimentally exposed. The pulps were capped with MTA alone as a pulp-capping agent and final restoration or with BMP-7 followed by restoration with MTA. Five teeth with class I occlusal preparations, no exposure, and no restoration served as positive controls. Five teeth that received pulp exposures and no restoration served as negative controls. Five untreated third molars served as additional controls. The animals were killed at 2 weeks. The specimens were prepared and evaluated histologically and with immunohistochemistry using polyclonal antibodies raised against rat DSP. Pulps capped with MTA formed hard tissue that demonstrated significantly more immunostaining for DSP compared with BMP-7 (p = 0.0031). MTA-capped pulps also showed significantly more complete bridge formation compared with BMP-7 (p = 0.0008). Pulps capped with BMP-7 demonstrated a hard tissue that was bone-like in appearance and devoid of DSP staining.

Aluminum Compounds↗

[Acute effect of calcium hydroxide-containing material in the rat's dental pulp microcirculation].

Experimental stimulation and clinical procedures applied on the crown of the tooth cause vascular reaction in the dental pulp. Laser-Doppler flowmetry is a good method for determining the blood flow of the dental pulp. The aim of these experiments was to study the acute effect of a calcium hydroxide-containing pulp-capping material (Dycal, DeTrey) on blood flow of the dental pulp after the application into a deep test-cavity. Two groups of male Sprague-Dawley rats (308 g +/- 50 S.E.) were used: control- and test-group, n = 10. A standardised deep class five cavity was prepared in the left lower incisor of each rat. Laser-Doppler flowmeter was used to measure the vascular reaction of the pulp. The levels of blood flow were recorded prior to (0 min.) and after the application (1, 5, 15, 30, 60 min.) of Dycal. Results were evaluated with one-way ANOVA. Significantly higher pulpal blood flow was found only in the first minute after the application of Dycal. In any other time no significant difference was found between the results before and after (5, 15, 30, 60 min.) the application in test- and control-group.

Analysis of Variance↗

Octacalcium phosphate-based cement as a pulp-capping agent in rats.

OBJECTIVE: The purpose of this study was to evaluate the pulpal response to an octacalcium phosphate (OCP)-based cement used as a pulp-capping material. STUDY DESIGN: The pulps of 60 maxillary first molars of male Sprague-Dawley rats were exposed and then capped directly by using either OCP-based cement or a calcium hydroxide slurry (control). Histologic examinations were performed at 1, 2, and 5 weeks after the surgical procedure, and the results were analyzed statistically by using the Mann-Whitney U test (P<.05). RESULTS: One week after pulp capping, the initial formation of reparative dentin in the exposed areas was more notable in the calcium hydroxide group than in the OCP-based cement group. At 2 weeks, reparative dentin covered by a layer of odontoblast-like cells was observed in both groups. However, at 5 weeks, reparative dentin consisting of regular dentinal tubules was observed more frequently in the OCP-based cement group. CONCLUSION: OCP-based cement allowed favorable healing processes to occur in the dental pulp.

Animals↗

Indirect pulp capping: a survey.

This study addresses the acceptance of the clinical practice of indirect pulp capping. State and regional dental boards and postgraduate dental education programs throughout the United States were surveyed. Results indicate that no clear consensus exists for the acceptance of this clinical procedure.

Dental Pulp Capping↗