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PubMed1977

[Clinical and bacteriological study on Flavobacterium meningosepticum (author's transl)].

The present study concerns in vitro observations on the susceptibility to antibiotics and chemotherapeutic agents of the strains of F. meningosepticum isolated from various clinical specimens at the Clinical Laboratory of Juntendo University Hospital and the assessment of their clinical significance of the patient, when they are isolated from clinical specimens. 1) Fifty-two % of the strains was isolated from sputum, 20% from urine and 8% from pus and exudate. Only 2 strains were obtained from blood and one strain from cerebro-spinal fluid. 2) The patients with F. meningosepticum from sputum had respiratory distress due to the basic disorder of central nervous system, respiratory system or cardiovascular system. An endotracheal tube or a tracheal canule was placed in most of the patients to assure a patient airway, and they received massive antibiotic therapy. Most of the patients with infected urine had functional and structural abnormality of the urinary tract, as well as a history of instrumentation, and had previously received antibiotic therapy. The bacteremia due to F. meningosepticum occurred after major operation in 2 patients, and one of them suffered from hydrocephalus associated with meningitis, moreover, in whom the organism was isolated from cerebro-spinal fluid. The route of infection with F. meningosepticum cannot be assessed certainly, but the correlation with catheterization or canulation strongly suggests that the instrumentation is required for the organism to become established. 3) In vitro test for sensitivity to 19 chemotherapeutic agents were done with the strains of F. meningosepticum isolated at the laboratory from August 1974 through July 1976. A large number of the strains were highly resistant to SB-PC, CB-PC, DKB, LCM, CL and NF, and resistant to AB-PC, CEZ and AMK. All strains were highly sensitive to MINO, DOTC and CLDM in this order, and were sensitive to EM, PA and NA. The relative sensitivity was found to TC, CP, GM and PPA.

Adult↗
PubMed2003

Vitellibacter vladivostokensis gen. nov., sp. nov., a new member of the phylum Cytophaga-Flavobacterium-Bacteroides.

A novel heterotrophic, yellow-orange-pigmented, non-motile, asporogenic, strictly aerobic, Gram-negative, oxidase and catalase-positive bacterium KMM 3516(T) was isolated from the holothurian Apostichopus japonicus collected from Troitsa Bay in the Gulf of Peter the Great (Sea of Japan) during November 1997. 16S rDNA sequence analysis revealed that strain KMM 3516(T) was a member of the family FLAVOBACTERIACEAE: The DNA G+C content of KMM 3516(T) was 41.3 mol%. Major respiratory quinone was MK-6. Predominant fatty acids were i15 : 0 and alpha15 : 0 (68.8 and 8.4 %, respectively). On the basis of phenotypic, chemotaxonomic, genotypic and phylogenetic characteristics, the novel bacterium has been designated Vitellibacter vladivostokensis gen. nov., sp. nov. The type strain is KMM 3516(T) (=NBRC 16718(T)).

Base Composition↗
PubMed2004

Aquiflexum balticum gen. nov., sp. nov., a novel marine bacterium of the Cytophaga-Flavobacterium-Bacteroides group isolated from surface water of the central Baltic Sea.

A bacterial isolate from the Baltic Sea, BA160(T), was characterized for its physiological and biochemical features, fatty acid profile, G+C content and phylogenetic position based on 16S rRNA gene sequences. The strain was isolated from the surface water of the central Baltic Sea during the decay of a plankton bloom. Phylogenetic analyses of the 16S rRNA gene sequence revealed a clear affiliation with the family 'Flexibacteraceae', and showed the closest phylogenetic relationship with the species Belliella baltica and Cyclobacterium marinum. The G+C content of the DNA was 38.4 mol%. The strain was red-coloured due to carotenoids, Gram-negative, rod-shaped, and catalase- and oxidase-positive. Growth was observed at salinities from 0 to 6 %, with an optimum around 1.5 %. Temperature for growth ranged from 4 to 40 degrees C, with an optimum around 30 degrees C. The fatty acids were dominated by branched-chain fatty acids (>87 %), with a high abundance of iso-C(15 : 0) (23 %) and anteiso-C(15 : 0) (19 %). According to its morphology, physiology, fatty acid composition, G+C content and 16S rRNA gene sequence, strain BA160(T) is considered to represent a new genus of the family 'Flexibacteraceae'. Due to its aquatic origin, the name Aquiflexum balticum gen. nov, sp. nov. is suggested for the type species (type strain, BA160(T)=DSM 16537(T)=LMG 22565(T)=CIP 108445(T)) of the new genus.

Bacterial Typing Techniques↗