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PubMed2002

Evaluation of the plug formation process of silicified microcrystalline cellulose.

To investigate the powder plug formation process of silicified microcrystalline cellulose (SMCC) under compression forces consistent with automatic capsule-filling machines, a single-ended saw-tooth wave was used to make powder plugs with different heights (6, 8, 12 mm), at two different punch speeds (1 and 50 mm/s) on a tablet compaction simulator. SMCC was compared to Starch 1500, anhydrous lactose (direct tableting grade), and microcrystalline cellulose. Heckel analysis showed that 'apparent mean yield pressures' (AMYP) of all tested materials increased with an increase in the plug height and punch speed. AMYP appeared to depend on the material type and punch speed. Not all materials fit the Shaxby-Evans relationship at such low compression forces (less than 250 N). Only SMCC 90, SMCC HD90 and anhydrous lactose data fit the equation at both punch speeds. Due to poor axial load transmission, the R values of all tested materials decreased with an increase in the plug height. The experimental data fit the Kawakita equation quite well. Overall, Kawakita's b values were inversely related to AMYP values. The maximum breaking force (MBF) of a 12 mm plug formed at a punch speed of 50 mm/s correlated well with the work of compaction, except for SMCC HD90 and SMCC X, which exhibited very high MBF values. This research demonstrated that several grades of SMCC produced plugs having higher MBF than anhydrous lactose and Starch 1500 under similar compression conditions. The apparently higher compactability of these materials at low plug formation forces may be beneficial in developing direct fill formulations for automatic capsule filling machines.

Capsules↗

PubMed1991

The generation and characterization of silicon carbide whiskers (fibers) for inhalation toxicology studies.

Atmospheres of silicon carbide whiskers (wh) were required for a repeated dose lung deposition study and a subchronic inhalation toxicity study, each involving exposure to three concentrations of whiskers for 6 hr/day, 5 days/week for 1 or 13 weeks. Target concentrations were 0, 500, 1500, and 7500 wh/cc. By using the mean fiber length (10 microns), diameter (0.555 micron), and density (3.2 g/cc) of the bulk material, target mass concentrations were approximately 0, 4, 12, and 60 mg/m3. The Pitt III generator, developed for cotton fiber dispersion at the University of Pittsburgh, was selected. This instrument consists of a vertical 18-in. long cylinder, which is closed at each end with rubber dams and coupled at the base to a loudspeaker. The sonic energy from the loudspeaker fluidizes the test material and the whiskers in the air column are carried out with the exhaust air. The output of the Pitt III generator was altered by changing the sound energy input, the rate of introduction of the test material into the fluidizing cylinder, or the airflow through the cylinder. Separate generation systems were used for each inhalation chamber. Chamber atmospheres were characterized gravimetrically and samples were analyzed by scanning electron microscopy. Mean mass and number concentrations for the subchronic study were 0.09, 3.93, 10.7, and 60.5 mg/m3 and 0, 630, 1746, and 7276 wh/cc, respectively. Weighted mean values for whisker diameters, lengths, and aspect ratios were 0.560 micron, 4.53 microns, and 8.62, respectively. Although whisker lengths were less than half those of the bulk material, nearly 30% of the whiskers were greater than 5 microns long.

Administration, Inhalation↗

PubMed2005

A technique for field measurement of knife sharpness.

Knife sharpness can affect the productivity of meatpacking operations as well as the forces to which meat cutters are exposed. This report describes the development and evaluation of a "sharpness tester" designed to meet the criteria that the system be portable for field use at meatpacking plants, requires no special skills to operate, provides a non-destructive test of the entire blade edge, and incorporates a test motion that is representative of meat cutting. The system was bench tested for sensitivity to incremental changes in blade sharpness; suitability of the test material as a surrogate for red meat; and ability to detect variations in sharpness along the length of the blade. A graphical presentation showed that changes in cutting forces measured with the system correspond to changes in blade sharpness. Measurements made with both the test material and with red meat showed good correlation (r = 0.89). The system demonstrated the capacity to detect differences in sharpness in regions of a knife edge as well as providing visual evidence of defects along the edge of a knife blade.

Equipment Design↗

PubMed2001

Fine thread versus coarse thread. A comparison of the maximum holding power.

For osteosynthesis in the epiphyseal region of long bones, specialized screw types are available to achieve a better purchase in cancellous bone. Typically, the cancellous bone screw has a large outer thread diameter with a relatively small core diameter and a great pitch. Due to its dimensions, this screw type is not suited for the cancellous bone areas in hand and foot surgery, respectively. Fine machine screws are better adapted to the bone dimensions in this part of the skeleton. To compare the holding power of fine machine screws with the holding power of other screw types available in the market, a biomechanical study was undertaken. Different screw types were compared: 4 mm cancellous screw, 3.5 and 2.7 mm cortical screws and three different diameters of a fine machine screw (2.2, 1.6 and 1.2 mm). Both cortical screws were tested in pretapped and self-tapping version. Polyurethane foam and bovine cancellous bone were tested. The screws were applied according to the manufacturer's instructions and an axial extraction force was applied. The 4 mm cancellous bone screws were superior to all the other screws in both test materials (P<0.05). The 3.5 mm cortical screw showed a significant difference compared to the 2.7 mm cortical screw in both materials. A significant difference between the self-tapping and pretapped screws of the same diameter was only found in the 3.5 mm cortical screws in polyurethane foam (P<0.05). Interestingly, the maximum holding power of the 2.2 mm fine machine screws in homogeneous test material (polyurethane foam) was as high as the holding power of the 2.7 mm cortical screws (P<0.05). In natural bovine cancellous bone, the result was even more striking. There was no significant difference between the 2.2 mm fine machine screws and the 3.5 mm cortical screws (P<0.05). Relative to their thread diameter, the fine machine screws were superior to conventional cancellous and cortical screws in cancellous bone. The high holding power of this screw type is at least partly due to the impaction effect of the drilling debris between the thread and the abutting spongy bone structure.

Animals↗

PubMed1991

[The effect of adhesive agents on the bonding strength of ceramic-composite. An in-vitro comparative study with 6 "porcelain primers" and a control group].

Orthodontic brackets were bonded to a total of 240 Biodent porcelain surfaces pretreated with a number of different porcelain primers. In a control group, 40 surfaces were pumiced, etched, rinsed and dried, and then bonded to similar brackets without the use of a primer. The test objects of group 1 (n = 140) and those of group 2 (n = 40) were tested for bond shearing strength in a material testing machine (Zwick 1441) after prior incubation in a corrosive fluid at a pH of 2.3 and a temperature of 37 degrees C for 24 hours and 21 days, respectively. The fracture sites were inspected and analysed. Incubation in corrosive fluid reduces the strength of the porcelain-composite bonding, the shearing strength and the number of fractures in the bonding decreasing. Damage to the porcelain was seen to occur after the use of Lee-Ortho-Prime and Bond-Por primers. All the primers tested can be used, although a bond shearing strength of less than 2 N/mm2 is at the lower limit of what can be accepted under the usual conditions of daily practice.

Ceramics↗

PubMed1993

Influence of ambient temperature on true digestibility of protein and amino acids of rapeseed and soybean meals in broilers.

The effect of high ambient temperature (32 versus 21 C) between 4 and 6 wk of age on true digestibility of protein (TDP), amino acids (TDAA), and AME value of rapeseed and soybean meals was investigated in broilers. At 6 wk of age, 96 male and female birds were deprived of feed for 24 h and then force-fed with moistened diet (approximately 90 g). Excreta were collected during the two subsequent 24-h periods after force-feeding. The results of this experiment showed that the ambient temperature had no effect on AME values of the raw materials tested for both sexes of broilers. However, high ambient temperature significantly decreased (P < .001) AMEn values of the two rapeseed meals, which could be related to the nitrogen balance. Moreover, TDP and TDAA of two rapeseed and soybean meals tested in this experiment were decreased as the ambient temperature increased from 21 to 32 C. A 12% reduction in TDP value was observed with the rapeseed meals, whereas the diminution was only 5% with the soybean meal. Sex had no effect on TDP of the raw materials tested. However, for TDAA values, female birds appeared to be more sensitive to high ambient temperature than male chickens when fed rapeseed meals.

Amino Acids↗

PubMed2001

Fatigue testing median sternotomy closures.

OBJECTIVE: Sternal dehiscence is commonly due to wire cutting through bone. With a biological model, we measured the rate of cutting through bone, of standard steel wire closure, peristernal steel wire, figure-of-eight closure, polyester and sternal bands sternotomy closure techniques. METHODS: Polyester, figure-of-eight, peristernal and sternal band closures were tested against standard closure eight times using adjacent paired samples, to eliminate biological variables. Fatigue testing was performed by a computerized materials-testing machine, cycling between loads of 1 and 10 kg. The displacements at maximum and minimum loads were measured during each cycle. Cutting through, manifested by the displacement at the maximum load between the 1st and 150th cycles was measured. The percentage cut-through of each closure method versus standard closure was calculated. RESULTS: The differences in the displacement between each of the polyester (1.01 mm), figure-of-eight (0.52 mm), peristernal (0.72 mm) and sternal band (0.66 mm) groups versus standard closure (0.22, 0.22, 2.1, 3.2 mm) in the paired samples were statistically significant (Student's paired t-test; P<0.01). There were statistically significant differences in the percentage cut-through of polyester, figure-of-eight, peristernal and sternal bands (ANOVA, P<0.001), versus standard closure. CONCLUSIONS: In our sheep sternum model, we have quantified the differing rate of cutting through bone of five types of median sternotomy closure techniques. We have controlled for bone variables by testing each closure versus standard closure using paired adjacent bone samples. Peristernal and sternal band closure techniques are significantly superior to standard closure. The use of polyester and figure-of-eight closures requires caution.

Analysis of Variance↗

PubMed2005

Rotational stability of Seidel nail distal locking mechanism.

The intramedullary distal locking mechanism in the Seidel nail uses interference fit, avoiding the need of a screw traversing soft tissue and the risk of neurovascular injury. However, there is concern for its rotational stability. This study was designed to test the rotational stability of the distal locking mechanism under the least stable conditions. A transverse osteotomy was performed 10 cm proximal to the trochlea in 10 cadaveric humeri, followed by insertion of a 9 mm diameter Seidel nail and distally locked. The proximal humeral segments were removed. Rotational test to failure was performed with a material testing machine. The initial medullary diameter and the amount of flange spread measured 9.95 mm and 11.28 mm, respectively. Most of the flanges were found to be incompletely deployed. Failure occurred by slippage of the flanges. The average failure torque and torsional stiffness of the nail bone construct tested were 0.262 N m and 0.070 N m/ degrees . The Seidel nail distal locking mechanism has low rotational stability on mechanical testing. However, the low failure torque tested in vitro does not always preclude successful fracture healing and a good clinical outcome. Whether the presence of rotational micro motion would be beneficial to bone healing would need further investigation.

Adult↗

PubMed1993

Patch test reactions to mite antigens: a GERDA multicentre study. Groupe d'Etudes et de Recherches en Dermato-Allergie.

We performed patch tests with Dermatophagoides pteronyssinus (Dp) antigens from 2 different sources in 355 non-randomly selected patients with atopic dermatitis (AD) and 398 subjects of a control group. The study demonstrated that contact sensitization to mites occurred in an appreciable % of AD cases (20.8%), using commonly available assay products. The differences recorded between the 2 materials tested were related to the concentration of P1 antigen. Non-atopic patients rarely showed positive reactions to Dp (0.75%), when strict criteria for readings were applied and if 2 readings were performed. Patients with positive patch tests did not necessarily show positive immediate skin tests. It would be useful to carry out tests systematically in atopic patients, even if it is not yet known what modern treatment would be best for the patient. Laboratories still do not provide standardized house dust mite preparations--measuring and codifying their biological activity--for use in patch tests. It is to be hoped that the extension of this type of test will lead to the production of better test materials, in syringes with homogeneous dispersion and concentration.

Administration, Inhalation↗

PubMed1990

A predictive mouse ear-swelling model for investigating topical photoallergy.

The photoallergic potential of various compounds was assessed using a mouse ear-swelling model, which offers the advantage of being quantifiable and more objective than models based on subjective evaluation of erythematous skin reactions. Cyclophosphamide pretreated BALB/c mice were induced by topical treatment of the dorsal skin surface on 3 consecutive days and challenged on the ears 5 days after the last induction. For each induction and challenge treatment, mice were consecutively irradiated with ultraviolet (UV) A (10 J/cm2) and UVB (45 mJ/cm2) radiation 30 min to 1 hr after test material application. The photoallergic response to musk ambrette, a known human photoallergen, was significantly augmented when three consecutive induction exposures were used as compared with one or two inductions. The photoallergic potential of nine other known human photoallergens (tetrachlorosalicylanilide, bithionol, 6-methylcoumarin, chlorpromazine, sodium omadine, bisphenol A, sulphanilamide, fentichlor and p-aminobenzoic acid) was successfully detected using the mouse model. In each experiment, the ear thickness changes observed in the photoallergy test mice were significantly greater than the changes observed in the contact allergy, vehicle/radiation and phototoxicity control mice. Coumarin and homosalate, two agents not traditionally associated with causing photoallergy in humans or animals, did not demonstrate contact photoallergy using this model. With three of the photoallergens, musk ambrette, bithionol and tetrachlorosalicylanilide, the ear swelling response obtained was due to photoallergy alone and not due to the co-existence of both contact photoallergy and contact allergy. In addition, irradiating mice 24 hr, rather than 1 hr, following application of the test material during the induction phase resulted in a significantly reduced photoallergic response with both musk ambrette and tetrachlorosalicylanilide. These results indicate that the mouse ear-swelling model is a potentially useful model for investigative and predictive photoallergy testing.

Administration, Topical↗

PubMed2006

A mechanical study of ligature security of commercially available pre-tied ligatures versus hand tied ligatures for use in equine laparoscopy.

OBJECTIVE: To determine a hand-tied ligature knot configuration, suture size, and suture type that would be an acceptable substitute to commercially available ligature knots for use in equine laparoscopic surgery. STUDY DESIGN: Three-factor ANOVA with the fixed effects being suture type, suture size, and knot type. The dependent variable was ligature security (load to failure). METHODS: Commercially available Endoloop and 4 hand-tied slipknot ligatures were compared with a 4-layer square knot. The hand tied ligature knots tested were the 4S modified Roeder knot, the Brooks knot, the modified Roeder knot, and the Weston knot. Two suture sizes (0, 2) of each of 2 suture types (polydioxanone, polyglactin 910) were tested with each hand tied knot. Two types (polydioxanone, polyglactin 910) of size 0 Endoloop were tested. Twenty repetitions of each knot suture type were tested for force to yield on a materials testing machine. Statistical analysis consisted of a 3-way ANOVA with individual comparisons made using the Bonferroni method. Significance was P< or = .05. RESULTS: Only the 4S modified Roeder knot in 2 polydioxanone (103 N) was comparable with the breaking strength of the square knots (157 N). All other knot suture combinations tested were significantly weaker than the square knot (range, 2-18 N). CONCLUSION: For equine laparoscopy, a 2 polydioxanone 4S modified Roeder knot would be an acceptable alternative to the commercially available Endoloop. CLINICAL RELEVANCE: Laparoscopic slip knots are commonly used in equine laparoscopic surgery. Various knot configuration and suture size and type combinations will affect the security of the slip knot.

Analysis of Variance↗

PubMed1995

The effect of adhesive luting agent-dentinal surface interactions on compressive strength of two types of all-ceramic crowns.

This research compared the compressive strength of two types of all-ceramic crown (Hi-Ceram and Duceram) as affected by selected luting cements (Zn phosphate, glass ionomer and composite resin cement). Thirty crowns of similar size and shape were constructed (15 crowns of each tested material) to fit a standard posterior tooth preparation. Five crowns from each material were cemented by one of the tested cements. The cemented crowns were loaded until catastrophic failure. A two-way analysis of variance was performed and showed that the type of utilized cement had a significant effect on the compressive strength being that Panevia Ex. resin cement the most effective one followed by glass ionomer and then finally zn phosphate cement. Statistical analysis also showed that Hi-Ceram crowns were more resistant to occlusal load than Duceram.

Aluminum Oxide↗

PubMed1993

Extraction strength of cannulated cancellous bone screws.

The purpose of this study was to determine whether the alterations in the relative dimensions of thread and core diameter that accompany cannulation of cancellous bone screws affect their holding strength. The screws were inserted in bovine femoral cancellous bone and mounted in the load train of a materials testing system. They were then extracted under servo-hydraulic displacement control. The tests were repeated in synthetic cancellous material. From the corresponding load-displacement histories, the maximum force values attained were determined as the screws' holding strengths. The results of tests from 17 matched pairs of bovine femora, and of 15 tests of each screw in synthetic material, were recorded. No significant differences in extraction strength between 7.0-mm cannulated and 6.5-mm standard cancellous bone screws were found. Based on tests in the bovine femur and synthetic cancellous material, this study showed that the extraction strength of cancellous bone screws is not altered by the changes in thread-to-core ratio accommodating cannulation.

Animals↗

PubMed2005

Zone I flexor digitorum profundus repair: an ex vivo biomechanical analysis of tendon to bone repair in cadavera.

PURPOSE: Biomechanical studies of standard flexor digitorum profundus (FDP) tendon to bone repairs show ultimate strengths greater than the applied loads of early motion rehabilitation protocols. Strain data, however, indicate the potential for significant repair site gapping under these physiologic loads. Gaps in excess of 3 mm have been shown to prevent the time accrual of strength in midsubstance tendon repairs and may prevent the restoration of the normal architecture of the tendon-bone interface. Improving the time-zero tensile properties of FDP insertion site repairs may help obviate these issues and improve clinical outcomes. The purpose of this study was to evaluate the ex vivo biomechanical properties of 2 new repair techniques in comparison with the standard FDP tendon to distal phalanx cortical surface repair. METHODS: Thirty human cadaver FDP tendons were released from their insertion sites by sharp dissection and repaired to bone using 1 of 3 repair techniques. Load to failure testing was performed with a servohydraulic materials-testing system (model 8500R; Instron, Canton, MA) analyzing ultimate force, strain at 20 N, rigidity, force to 2-mm gap formation, and displacement at failure. RESULTS: The results of the failure tests indicate that repairs performed with the addition of a peripheral suture had a greater ultimate force, had increased resistance to gap formation, and had increased rigidity and decreased strain at 20 N compared with the tunnel-only and volar cortical surface to the distal phalanx repairs. Although there were no statistically significant differences in ultimate force or rigidity between the tunnel-only and volar cortical surface repairs, the tunnel-only repairs showed lower strain values and increased values for resistance to 2-mm gap formation when compared with the volar cortical surface repairs. There were no differences among any of the repair groups with regard to the magnitude of tendon displacement from the repair site at failure. CONCLUSIONS: The addition of a peripheral suture to the FDP tendon to bone tunnel repair construct improves the time-zero tensile properties as evidenced by statistically significant increases in ultimate force, rigidity, and resistance to gap formations of 2 mm. In comparison with a volar cortical surface repair, the bone tunnel-only repairs were effective at decreasing the amount of repair site strain during applied loads of 20 N. If these improved time-zero tensile properties persist during the early stages of healing, they may help decrease the incidence of repair-site gap formation associated with the forces of early motion rehabilitation protocols.

Aged↗

PubMed1999

Hepatic pyridine nucleotides content in rat--a better indicator for determining available niacin values of food.

After 10 days depletion period with niacin free diet, weanling rats were repleted with either reference diets containing niacin at four levels (2, 4, 8 and 12 mg/kg diet) or test diets containing test material at two levels as source of niacin. Gains in body weight and hepatic pyridine nucleotides content increased with increase in niacin intake. The correlation coefficient for hepatic pyridine nucleotides content and niacin intake was 0.98, whereas for weight gain and niacin intake was 0.89. Dried skim milk and pearl millet were taken as test materials. Dried skim milk with most of its niacin in free form showed 98% niacin equivalent to be bioavailable whereas in pearl millet niacin was in bound form and bioavailability equivalent was only 48 per cent.

Animals↗

PubMed2004

Study of effect of adsorptive building material on formaldehyde concentrations: development of measuring methods and modeling of adsorption phenomena.

UNLABELLED: In this paper, a method is developed to assess the performance of adsorptive building materials that are used for reducing indoor pollutant concentrations. Mass transfer has a great influence on the materials' performance. To control the mass transfer rate precisely in the performance test, the authors have developed the Boundary-Layer-Type Small Test Chamber in which airflow along the test materials can be controlled precisely. A new index of adsorption performance, the equivalent ventilation rate (Q(ads)), is defined that corresponds to the mass transfer coefficient when the surface pollutant concentration is zero. Modeling and experimental verification of adsorption were done, demonstrating the pollutant concentration decrease caused by adsorptive building materials. The pollutant reduction phenomena were modeled, including pollutant degradation by chemical reaction and adsorption in building materials. Adsorption tests of gypsum board containing a substance that decomposes HCHO within the board are reported. The adsorption rate of the gypsum board predicted by numerical analysis (CFD, Computational Fluid Dynamics) corresponds well with experimental results. PRACTICAL IMPLICATIONS: Development and verification of a method to measure the decrease in indoor pollutant concentration caused by an adsorptive building material are reported. Mass transfer has a great influence on the material's performance. The equivalent ventilation rate (Q(ads)) of the adsorption performance is defined as a new index that corresponds to the mass transfer coefficient. The equivalent ventilation rate (Q(ads)) can be used directly to compare the effect of pollutant concentration decrease via adsorption with the effect of ventilation.

Adsorption↗

PubMed1993

Toxicity of L-ascorbic acid to L929 fibroblast cultures: relevance to biocompatibility testing of materials for use in wound management.

Fibroblast cultures are often used to evaluate materials intended for medical use, cytotoxicity being taken as an indicator of bioincompatibility. Such an approach has previously been taken with ascorbic acid in determining its value in wound healing. We have now reexamined the toxicity of L-ascorbic acid to L929 fibroblast cells in culture. Concentrations of ascorbic acid between 0.5 mM and 11 mM were tested. At concentrations above 2 mM, ascorbic acid was found to inhibit cell proliferation, with cell viability decreasing as the concentration was increased. This effect could be prevented by the addition of either superoxide dismutase or catalase to the culture medium. Assays of glutathione and glutathione disulfide were carried out on 8 day old cultures exposed for 24 h to the same concentrations of ascorbic acid. A dose-related depletion of glutathione occurred whilst glutathione disulfide levels remained essentially constant. Lactate dehydrogenase and glucose-6-phosphate dehydrogenase activities were induced by ascorbic acid at all concentrations tested but the ratio of NADP to NADPH nevertheless increased as the concentration of ascorbic acid increased. Finally, ATP in cells from 8-day-old cultures became depleted in the presence of ascorbic acid at concentrations in excess of about 5 mM when assayed after 24 h incubation. These biochemical changes and the concomitant cytostatic/cytotoxic effects may be ascribed to the reactive oxygen species produced by the autoxidation of ascorbic acid in the culture medium. Ascorbic acid breakdown products appeared not to be directly involved. In addition, our results suggested that superoxide acted cooperatively with hydroxyl to elicit these effects on the fibroblasts. It is evident from this study that the microenvironment surrounding fibroblasts in culture may differ fundamentally from that surrounding fibroblasts in a healing wound, making it impossible to extrapolate directly to an in vivo situation and hence to make any recommendations from these results concerning the use of ascorbic acid in wound healing.

Adenosine Triphosphate↗

PubMed2004

Anthelmintic efficacy of genistein, the active principle of Flemingia vestita (Fabaceae): alterations in the free amino acid pool and ammonia levels in the fluke, Fasciolopsis buski.

The crude root-peel extract of Flemingia vestita, its active principle genistein and the reference flukicide oxyclozanide were tested against Fasciolopsis buski, the giant intestinal trematode. The amino acid composition of F. buski was demonstrated using HPLC and it was observed that the free amino acid (FAA) pool of the control worm consisted of aspartate, threonine, serine, glutamic acid, glutamine, proline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, lysine, histidine, arginine, phosphoserine, taurine, citrulline, ornithine, beta-alanine, and gamma-amino butyric acid (GABA). Of the amino acids detected valine was found to be the maximum in quantitative analysis. In qualitative analysis the FAA pool of the parasites under various treatments remained same as that of the control; however, quantitatively the level of various FAAs in the parasite was significantly affected. The treated parasites showed a marked decrease in the levels of arginine, ornithine, tyrosine, leucine, isoleucine, valine, alanine, glycine, proline, serine, threonine, and taurine following treatment with 20 mg/ml of crude peel extract, 0.5 mg/ml of genistein and 20 mg/ml of the reference drug, though an increase in the levels of glutamic acid, glutamine, phosphoserine, citrulline and GABA was noticeable. Enhanced levels of GABA and citrulline under the influence of genistein may be implicated in alterations of nitric oxide release and consequent neurological change (e.g. paralysis) in the parasite. Ammonia in the tissue homogenate as well as in the incubation medium showed a quantitative increase compared to the controls after treatment with the various test materials. The ammonia level increased by 40.7%, 66.4% and 18.16% in treatments with F. vestita, genistein and oxyclozanide, respectively, at the mentioned dosages. The changes in the levels of the amino acids and nitrogen components post treatment suggest that the amino acid metabolism in the parasite may have been altered under the influence of the test materials.

Amino Acids↗