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At least 703 records · Page 39Linked to original sources

The effect of laser microsurgery on cytoplasmic strands and cytoplasmic streaming in isolated plant protoplasts.

Cytoplasmic strands of actively streaming isolated callus derived protoplasts of higher plants were subjected to laser microsurgery. Typically, the irradiated strand retracted and cytoplasmic streaming stopped in the entire cell. At the same time, all cytoplasmic strands disintegrated, and formerly not totally spherically shaped protoplasts became spherical. This result indicates tension as a stability factor of cell shape and the cytoplasmic make-up and demonstrates that the nonspherical shape of these protoplasts is not due to cell wall residues. Cytoplasmic streaming as well as the strands were quickly reestablished and often the irregular shape of the protoplast appeared again. Treatment of unirradiated cells with cytochalasin B showed similar effects. The results are discussed with respect to the involvement of cytoskeletal elements in cytoplasmic streaming and organization.

Cytochalasin B↗

Anastomosis of the freshly divided uterine horns of rats with the CO2 laser vs. microsurgery.

We used the CO2 laser (group 1) and conventional microsurgery (group 2) for anastomosis of the freshly divided uterine horns of rats and compared the two methods. Each group was then compared with a control group in whom only exploration was carried out at laparotomy. Comparison was done regarding the clinical and histologic results. In addition, serum levels and tissue concentrations of alkaline phosphatase (ALP) and lactic dehydrogenase (LDH) were measured, and the three groups were compared. No significant difference was found between the mean adhesion scores of groups 1 and 2; however, when the control group was compared with the other groups, the differences were statistically significant. The tubal patency rates in groups 1 and 2 and the control group were 83.3%, 79.2% and 100%, respectively, and the pregnancy rates in those groups were 54.5% (6/11), 45.5% (5/11) and 100% (10/10). The differences in tubal patency and pregnancy rates between groups 1 and 2 were not significant, but when each was compared with the control group, the differences were significant. The mean scores for mucosal regeneration and disruption of the muscularis layer in group 1 were significantly lower than those in group 2. Serum levels and tissue concentrations of ALP and LDH in the control group were lower than in groups 1 and 2, and the differences between the control group and each of the other groups were significant; however, no significant difference was found between groups 1 and 2.(ABSTRACT TRUNCATED AT 250 WORDS)

Anastomosis, Surgical↗

[Transanal endoscopic microsurgery (TEM): indications and limitations].

Sessile polyps of the proximal and middle part of the rectum are difficult and sometimes impossible to remove by snaring techniques. The conventional transanal approach to this level results in inappropriate survey and security. Parasacral techniques and low anterior resection require disproportionate measures to treat these adenomas. Transanal endoscopic microsurgery (TEM) is a relatively new operative technique to remove sessile polyps of the entire rectum, developed by Buess et al. [1] in Germany. The system uses a special endorectal scope connected to a stereoscopic angulated optical system for visualization. A continuous pressure-controlled insufflation of carbon dioxide keeps the rectum open for exposure. The excision is done by electrocautery in the submucosal plane or as full thickness excision in the submuscular plane. The defect is closed with an intrarectal running suture. Our report demonstrates the indications and limits of this technique and compares it to conventional techniques mentioned above. 12 patients with different kinds of tumor have been selected and serve as basis for our discussion. TEM offers excellent survey in the entire rectum and permits removal of villous adenomas and incidental low risk and early rectal cancers. High risk patients tolerate this procedure well. Unsuitable are circular and long-distance adenomas and transmural growing benign tumors of the proximal rectum.

Adenoma, Villous↗

Motorized teleguided stereotactic micromanipulator for vitreous microsurgery.

A recently developed motorized stereotactic micromanipulator enhances the precision of the procedure, the comfort of the surgeon, and the safety of the patient during vitreous surgery. It can accommodate almost any type of vitreous surgical instrument by means of special adapters. The system is moved by the action of electrical motors remotely controlled by the surgeon operating a joystick on a separate control unit. Electrical reversal of motion, obtained by flipping a switch, allows observation through an altered version of the Panfundoscope that provides a 160 degrees inverted fundus view during all phases of the operation. In addition to improving many aspects of present vitreous surgical procedures, this stereotactic system may initiate the development of still finer surgical methods that cannot be performed with the free hand.

Fluorescein Angiography↗

Evaluation of a telerobotic system to assist surgeons in microsurgery.

A tool was developed that assists surgeons in manipulating surgical instruments more precisely than is possible manually. The tool is a telemanipulator that scales down the surgeon's hand motion and filters tremor in the motion. The signals measured from the surgeon's hand are transformed and used to drive a six-degrees-of-freedom robot to position the surgical instrument mounted on its tip. A pilot study comparing the performance of the telemanipulator system against manual instrument positioning was conducted at the University of Southern California School of Medicine. The results show that a telerobotic tool can improve the performance of a microsurgeon by increasing the precision with which he can position surgical instruments, but this is achieved at the cost of increased time in performing the task. We believe that this technology will extend the capabilities of microsurgeons and allow more surgeons to perform highly skilled procedures currently performed only by the best surgeons. It will also enable performance of new surgical procedures that are beyond the capabilities of even the most skilled surgeons.

Evaluation Studies as Topic↗

Usefulness of microsurgery to isolation of recurrent laryngeal nerve and parathyroid during thyroidectomy operations.

The authors report their experience of the application of microsurgical techniques in total and subtotal thyroidectomy operations from January 1984-January 1997. Four hundred and eighty-six patients with thyroid diseases were operated upon with both traditional and microsurgical techniques. The comparison of results shows that microsurgical skills are effective in reducing the dangers and important complications in both laryngeal nerves and parathyroids.

Adolescent↗