Trialkyltins and oxidative phosphorylation. The [32P]phosphate-adenosine triphosphate-exchange reaction.
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Homogenates were prepared of the mucous membrane of the small intestine from dogs previously treated with high doses of reserpine. The granular material sedimented from these homogenates was centrifuged over a density gradient. The dense fraction usually rich in adenosinetriphosphate and 5-hydroxytryptamine contained little amine and no nucleotide; on the other hand, the less dense material, believed to consist mainly of mitochondria, contained amounts of adenosinetriphosphate similar to those found in animals not treated with reserpine.
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Under standard conditions of temperature, humidity, and (artificial) diet, loss in flight ability is paralleled by a decline of up to 66(2/3) percent in activity of enzymes dephosphorylating organophosphorous compounds in the thoracic flight muscle. Concomitantly, the content of adenosine triphosphate in the flight muscle increases five times, while the content of adenosine monophosphate correspondingly diminishes.
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