The use and limitations of the fluorescent antibody technic in the identification of bacteria in body fluids and exudates, and from cultures.
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Antigen, diffusing into converging edges of an antibody depot, inhibits the precipitation unless the reaction is completed before an inhibiting antigen excess is established. Since the rate of the increase of antigen concentration depends on the size of the angle, relative avidities of similar preparations of antibody may be estimated from the angle just producing observable inhibition.
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